(Circular) (Six-base) MAPSORT of: pFN21AB3427.seq Check: 4036 from: 1 to: 5980 pFN21AB3427 5980 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3790 278 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2458 3323 1937 Size: 521 865 4594 Fragments arranged by size: 4594 865 521 Acc65I G'GTAC_C Cuts at: 2603 3301 2603 Size: 698 5282 AclI AA'CG_TT Cuts at: 1563 2251 2515 2911 4107 4480 1563 Size: 688 264 396 1196 373 3063 Fragments arranged by size: 3063 1196 688 396 373 264 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3102 4043 5427 5695 1632 Size: 1470 941 1384 268 1917 Fragments arranged by size: 1917 1470 1384 941 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5783 AflIII A'CryG_T Cuts at: 1203 4879 5856 1203 Size: 3676 977 1327 Fragments arranged by size: 3676 1327 977 AgeI A'CCGG_T Cuts at: 4811 4811 Size: 5980 AhdI GACnn_n'nnGTC Cuts at: 4709 4709 Size: 5980 AleI CACnn'nnGTG Cuts at: 1166 2328 1166 Size: 1162 4818 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5980 AlwNI CAG_nnn'CTG Cuts at: 2972 3723 5295 2972 Size: 751 1572 3657 Fragments arranged by size: 3657 1572 751 ApaLI G'TGCA_C Cuts at: 2279 2789 4037 5193 2279 Size: 510 1248 1156 3066 Fragments arranged by size: 3066 1248 1156 510 ApoI r'AATT_y Cuts at: 1493 2108 3289 3515 3667 5776 1493 Size: 615 1181 226 152 2109 1697 Fragments arranged by size: 2109 1697 1181 615 226 152 AseI AT'TA_AT Cuts at: 160 1032 4534 160 Size: 872 3502 1606 Fragments arranged by size: 3502 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 AvaI C'yCGr_G Cuts at: 1870 1943 3305 1870 Size: 73 1362 4545 Fragments arranged by size: 4545 1362 73 BamHI G'GATC_C Cuts at: 3310 3677 3310 Size: 367 5613 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2603 3301 4756 618 Size: 325 217 627 816 698 1455 1842 Fragments arranged by size: 1842 1455 816 698 627 325 217 BanII G_rGCy'C Cuts at: 1391 3299 1391 Size: 1908 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5009 BbvCI CC'TCA_GC Cuts at: 2238 2238 Size: 5980 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4171 4205 1903 Size: 34 2234 34 3678 Fragments arranged by size: 3678 2234 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3234 3872 5082 3234 Size: 638 1210 4132 Fragments arranged by size: 4132 1210 638 BclI T'GATC_A Cuts at: 1548 1548 Size: 5980 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4591 136 Size: 107 122 71 631 605 337 2582 1525 Fragments arranged by size: 2582 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5975 5975 Size: 5980 BlpI GC'TnA_GC Cuts at: 3397 3397 Size: 5980 Bme1580I G_kGCm'C Cuts at: 1790 2283 2793 4041 5197 1790 Size: 493 510 1248 1156 2573 Fragments arranged by size: 2573 1248 1156 510 493 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5980 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3711 4669 442 Size: 416 53 83 399 24 2294 958 1753 Fragments arranged by size: 2294 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2775 1056 Size: 1719 4261 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2034 2231 2537 3201 4640 1239 Size: 795 197 306 664 1439 2579 Fragments arranged by size: 2579 1439 795 664 306 197 Bpu10I CC'TnA_GC Cuts at: 2238 2442 2238 Size: 204 5776 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2813 2854 4105 4970 5268 2813 Size: 41 1251 865 298 3525 Fragments arranged by size: 3525 1251 865 298 41 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4643 882 Size: 617 3144 2219 Fragments arranged by size: 3144 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2278 3771 493 Size: 711 1074 1493 2702 Fragments arranged by size: 2702 1493 1074 711 BsaBI GATnn'nnATC Cuts at: 5974 5974 Size: 5980 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3787 4169 4804 275 Size: 53 83 186 936 2254 382 635 1451 Fragments arranged by size: 2254 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2112 4413 4811 5085 5232 5643 2112 Size: 2301 398 274 147 411 2449 Fragments arranged by size: 2449 2301 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5755 Fragments arranged by size: 5755 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3230 1186 Size: 2044 3936 BseYI C'CCAG_C Cuts at: 5183 5679 5183 Size: 496 5484 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2475 3218 1163 Size: 573 739 743 3925 Fragments arranged by size: 3925 743 739 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3430 4191 5219 1540 Size: 275 186 1429 761 1028 2301 Fragments arranged by size: 2301 1429 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2283 2793 4041 4126 5197 2283 Size: 510 1248 85 1071 3066 Fragments arranged by size: 3066 1248 1071 510 85 BsmI GAATG_Cn' Cuts at: 3120 3491 3584 3120 Size: 371 93 5516 Fragments arranged by size: 5516 371 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2283 2793 3299 4041 4126 5197 1391 Size: 399 493 510 506 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 510 506 493 399 85 BspEI T'CCGG_A Cuts at: 2112 5643 2112 Size: 3531 2449 BspHI T'CATG_A Cuts at: 3869 5599 3869 Size: 1730 4250 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2400 2589 3335 844 Size: 1556 189 746 3489 Fragments arranged by size: 3489 1556 746 189 BsrBI CCG'CTC Cuts at: 1394 1823 2046 3867 1394 Size: 429 223 1821 3507 Fragments arranged by size: 3507 1821 429 223 BsrDI GCAATG_nn' Cuts at: 65 1248 4475 4649 65 Size: 1183 3227 174 1396 Fragments arranged by size: 3227 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4624 4811 1700 Size: 110 2814 187 2869 Fragments arranged by size: 2869 2814 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5980 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5980 BssSI C'ACGA_G Cuts at: 4040 5052 4040 Size: 1012 4968 BstAPI GCAn_nnn'nTGC Cuts at: 794 3133 794 Size: 2339 3641 BstBI TT'CG_AA Cuts at: 4784 4784 Size: 5980 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5980 BstYI r'GATC_y Cuts at: 1972 2536 2548 3018 3310 3677 4062 4079 5531 Size: 564 12 470 292 367 385 17 1452 Cuts at: 5531 5617 5629 5975 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 564 470 385 367 346 292 86 17 12 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3168 513 Size: 552 939 1164 3325 Fragments arranged by size: 3325 1164 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2891 487 Size: 683 56 1665 3576 Fragments arranged by size: 3576 1665 683 56 BtsI GCAGTG_nn' Cuts at: 2293 3496 4290 4310 2293 Size: 1203 794 20 3963 Fragments arranged by size: 3963 1203 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5980 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5945 DraI TTT'AAA Cuts at: 3284 3284 Size: 5980 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4987 809 Size: 507 3671 1802 Fragments arranged by size: 3671 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2290 3027 3144 Size: 54 1425 50 275 18 460 737 117 Cuts at: 3144 3165 3427 4316 5945 8 Size: 21 262 889 1629 43 Fragments arranged by size: 1629 1425 889 737 460 275 262 117 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 3427 1537 Size: 275 1615 4090 Fragments arranged by size: 4090 1615 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2238 3207 3910 1353 Size: 885 969 703 3423 Fragments arranged by size: 3423 969 885 703 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2159 4567 4941 5087 387 Size: 1064 138 570 2408 374 146 1280 Fragments arranged by size: 2408 1280 1064 570 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2034 2231 2537 3102 3201 4043 4640 Size: 393 402 197 306 565 99 842 597 Cuts at: 4640 5427 5695 1239 Size: 787 268 1524 Fragments arranged by size: 1524 842 787 597 565 402 393 306 268 197 99 EcoRI G'AATT_C Cuts at: 3289 3667 3289 Size: 378 5602 FspI TGC'GCA Cuts at: 4486 4486 Size: 5980 HaeII r_GCGC'y Cuts at: 1678 5127 1678 Size: 3449 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2186 2218 2521 2553 2697 Size: 32 135 32 614 32 303 32 144 Cuts at: 2697 2729 4621 4653 4695 4727 1373 Size: 32 1892 32 42 32 2626 Fragments arranged by size: 2626 1892 614 303 144 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3324 3570 669 Size: 988 281 1386 246 3079 Fragments arranged by size: 3079 1386 988 281 246 HindIII A'AGCT_T Cuts at: 748 2405 748 Size: 1657 4323 HpaI GTT'AAC Cuts at: 3570 3570 Size: 5980 KpnI G_GTAC'C Cuts at: 2607 3305 2607 Size: 698 5282 MfeI C'AATT_G Cuts at: 3579 3579 Size: 5980 MluI A'CGCG_T Cuts at: 5856 5856 Size: 5980 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2329 5094 5278 1305 Size: 1024 2765 184 2007 Fragments arranged by size: 2765 2007 1024 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2292 3146 3167 10 Size: 54 1425 343 460 854 21 2823 Fragments arranged by size: 2823 1425 854 460 343 54 21 MslI CAynn'nnrTG Cuts at: 518 1166 2328 3161 3167 3938 4297 4456 518 Size: 648 1162 833 6 771 359 159 2042 Fragments arranged by size: 2042 1162 833 771 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2128 2237 2497 3396 4073 5221 5466 1606 Size: 522 109 260 899 677 1148 245 2120 Fragments arranged by size: 2120 1148 899 677 522 260 245 109 NcoI C'CATG_G Cuts at: 513 1065 2004 3168 513 Size: 552 939 1164 3325 Fragments arranged by size: 3325 1164 939 552 NdeI CA'TA_TG Cuts at: 387 3241 387 Size: 2854 3126 NheI G'CTAG_C Cuts at: 1052 2771 1052 Size: 1719 4261 NotI GC'GGCC_GC Cuts at: 3427 3427 Size: 5980 NspI r_CATG'y Cuts at: 1132 1229 3338 4883 1132 Size: 97 2109 1545 2229 Fragments arranged by size: 2229 2109 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5980 PciI A'CATG_T Cuts at: 4879 4879 Size: 5980 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5980 PfoI T'CCnGG_A Cuts at: 2190 2190 Size: 5980 PmeI GTTT'AAAC Cuts at: 3284 3284 Size: 5980 PmlI CAC'GTG Cuts at: 2278 2278 Size: 5980 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4049 4081 5562 5594 4049 Size: 32 1481 32 4435 Fragments arranged by size: 4435 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2945 1613 Size: 1332 4648 PsiI TTA'TAA Cuts at: 3550 3550 Size: 5980 PstI C_TGCA'G Cuts at: 830 2314 2634 2921 3072 3332 830 Size: 1484 320 287 151 260 3478 Fragments arranged by size: 3478 1484 320 287 260 151 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5980 PvuII CAG'CTG Cuts at: 2128 2237 2497 2128 Size: 109 260 5611 Fragments arranged by size: 5611 260 109 SalI G'TCGA_C Cuts at: 1936 3322 1936 Size: 1386 4594 SbfI CC_TGCA'GG Cuts at: 3332 3332 Size: 5980 ScaI AGT'ACT Cuts at: 2757 4228 5940 2757 Size: 1471 1712 2797 Fragments arranged by size: 2797 1712 1471 SfcI C'TryA_G Cuts at: 826 1045 2310 2630 2917 2960 3068 3328 4463 Size: 219 1265 320 287 43 108 260 1135 Cuts at: 4463 5144 5335 826 Size: 681 191 1471 Fragments arranged by size: 1471 1265 1135 681 320 287 260 219 191 108 43 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5980 SmaI CCC'GGG Cuts at: 1872 3307 1872 Size: 1435 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2828 2869 4084 4985 5247 820 Size: 197 926 885 41 1215 901 262 1553 Fragments arranged by size: 1553 1215 926 901 885 262 197 41 SnaBI TAC'GTA Cuts at: 493 3771 493 Size: 3278 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5980 SphI G_CATG'C Cuts at: 1132 3338 1132 Size: 2206 3774 SspI AAT'ATT Cuts at: 5 52 3904 5 Size: 47 3852 2081 Fragments arranged by size: 3852 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5980 StyI C'CwwG_G Cuts at: 513 1065 2004 2326 3168 3419 513 Size: 552 939 322 842 251 3074 Fragments arranged by size: 3074 939 842 552 322 251 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4007 4024 4177 4362 4807 325 Size: 1255 2427 17 153 185 445 1498 Fragments arranged by size: 2427 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2755 4226 5938 Size: 274 80 33 51 1443 778 1471 1712 Cuts at: 5938 96 Size: 138 Fragments arranged by size: 1712 1471 1443 778 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3078 3110 5940 5972 3078 Size: 32 2830 32 3086 Fragments arranged by size: 3086 2830 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5980 XbaI T'CTAG_A Cuts at: 3316 3316 Size: 5980 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5980 XmaI C'CCGG_G Cuts at: 1870 3305 1870 Size: 1435 4545 XmnI GAAnn'nnTTC Cuts at: 4109 4109 Size: 5980 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3788 276 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI