Restriction map for
pFN21AB3422
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# NO CUT ENZYMES ( 189 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AvaII AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DdeI DpnII DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HphI KasI KpnI KspI
MamI MboI MfeI MflI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NcoI NdeI NdeII
NgoMIV NheI NlaIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacII SalI
Sau3AI Sau96I SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tsp509I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AfaI 211 GTAC GT^AC
Asp700I 253 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 7 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 94 CCCGAG C^[CT]CG[GA]G
BcnI 72 CCGGG CC^[GC]GG
BseRI 346 GAGGAG GAGGAG(10/8)
BsgI 239 CTGCAC GTGCAG(16/14)
BsoBI 94 CCCGAG C^[CT]CG[GA]G
BspHI 80 TCATGA T^CATGA
BstAPI 232 GCAAACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 69 CATCC GGATG(2/0)
BstNI 114 CCAGG CC^[AT]GG
DsaI 143 CCACGG C^C[GA][CT]GG
EaeI 134 CGGCCA [CT]^GGCC[GA]
EarI 84 GAAGAG CTCTTC(1/4)
EcoRII 114 CCAGG ^CC[AT]GG
EcoT14I 137 CCAAGG C^C[AT][AT]GG
FokI 69 CATCC GGATG(9/13)
HapII 72 CCGG C^CGG
HinfI 388 GACTC G^A[ACGT]TC
HpaII 72 CCGG C^CGG
Ksp632I 84 GAAGAG CTCTTC(1/4)
MaeII 8 ACGT A^CGT
MaeIII 371 GTGAC ^GT[ACGT]AC
MseI 320 TTAA T^TAA
MslI 187 CACGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 40 CAGCGG C[AC]G^C[GT]G
MspI 72 CCGG C^CGG
MvaI 114 CCAGG CC^[AT]GG
NciI 72 CCGGG CC^[GC]GG
PflFI 7 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 388 GACTC GAGTC(4/5)
RcaI 80 TCATGA T^CATGA
RsaI 211 GTAC GT^AC
SapI 84 GAAGAGC GCTCTTC(1/4)
ScaI 210 AGTACT AGT^ACT
SfcI 180 CTACAG C^T[GA][CT]AG
StyI 137 CCAAGG C^C[AT][AT]GG
Tru9I 320 TTAA T^TAA
Tsp45I 371 GTGAC ^GT[GC]AC
Tth111I 7 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 253 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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BanII 87 GAGCTC G[GA]GC[CT]^C
148 GAGCTC
BbvI 39 GCAGC GCAGC(8/12)
54 GCTGC
BfaI 166 CTAG C^TAG
299 CTAG
BsiYI 65 CCCGCATCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
66 CCGCATCCGGG
BslI 65 CCCGCATCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
66 CCGCATCCGGG
BssKI 72 CCGGG ^CC[ACGT]GG
114 CCAGG
FauI 65 CCCGC CCCGC(4/6)
32 GCGGG
HaeIII 135 GGCC GG^CC
141 GGCC
MaeI 166 CTAG C^TAG
299 CTAG
MwoI 45 GCTCATCTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
232 GCAAACACTGC
NlaIII 81 CATG CATG^
376 CATG
SacI 87 GAGCTC GAGCT^C
148 GAGCTC
ScrFI 72 CCGGG CC^[ACGT]GG
114 CCAGG
SfaNI 68 GCATC GCATC(5/9)
107 GCATC
TseI 39 GCAGC G^C[AT]GC
54 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
AciI AluI AspHI BmyI BsaJI
BsiHKAI Bsp1286I Fnu4HI ItaI MboII
MnlI TspRI