(Circular) (Six-base) MAPSORT of: pFN21AB3413.seq Check: 9641 from: 1 to: 5722 pFN21AB3413 5722 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3532 278 Size: 53 83 186 936 1996 2468 Fragments arranged by size: 2468 1996 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3065 1937 Size: 1128 4594 Acc65I G'GTAC_C Cuts at: 3043 3043 Size: 5722 AclI AA'CG_TT Cuts at: 1563 3849 4222 1563 Size: 2286 373 3063 Fragments arranged by size: 3063 2286 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2196 3033 3785 5169 5437 1632 Size: 564 837 752 1384 268 1917 Fragments arranged by size: 1917 1384 837 752 564 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5525 AflIII A'CryG_T Cuts at: 1203 4621 5598 1203 Size: 3418 977 1327 Fragments arranged by size: 3418 1327 977 AgeI A'CCGG_T Cuts at: 4553 4553 Size: 5722 AhdI GACnn_n'nnGTC Cuts at: 4451 4451 Size: 5722 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5722 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5722 AlwNI CAG_nnn'CTG Cuts at: 2629 3465 5037 2629 Size: 836 1572 3314 Fragments arranged by size: 3314 1572 836 ApaI G_GGCC'C Cuts at: 2347 2347 Size: 5722 ApaLI G'TGCA_C Cuts at: 3779 4935 3779 Size: 1156 4566 ApoI r'AATT_y Cuts at: 1493 3031 3257 3409 5518 1493 Size: 1538 226 152 2109 1697 Fragments arranged by size: 2109 1697 1538 226 152 AseI AT'TA_AT Cuts at: 160 1032 4276 160 Size: 872 3244 1606 Fragments arranged by size: 3244 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5722 AvaI C'yCGr_G Cuts at: 1870 1943 2306 2614 3047 1870 Size: 73 363 308 433 4545 Fragments arranged by size: 4545 433 363 308 73 BamHI G'GATC_C Cuts at: 2848 2860 3052 3419 2848 Size: 12 192 367 5151 Fragments arranged by size: 5151 367 192 12 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2478 3043 4498 618 Size: 325 217 627 691 565 1455 1842 Fragments arranged by size: 1842 1455 691 627 565 325 217 BanII G_rGCy'C Cuts at: 1391 2347 2496 2803 3041 1391 Size: 956 149 307 238 4072 Fragments arranged by size: 4072 956 307 238 149 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4751 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3913 3947 1903 Size: 34 1976 34 3678 Fragments arranged by size: 3678 1976 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3614 4824 3614 Size: 1210 4512 BclI T'GATC_A Cuts at: 1548 1548 Size: 5722 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4333 136 Size: 107 122 71 631 605 2661 1525 Fragments arranged by size: 2661 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5717 5717 Size: 5722 BlpI GC'TnA_GC Cuts at: 3139 3139 Size: 5722 Bme1580I G_kGCm'C Cuts at: 1790 2138 2347 3783 4939 1790 Size: 348 209 1436 1156 2573 Fragments arranged by size: 2573 1436 1156 348 209 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5722 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2563 2715 3453 Size: 416 53 83 399 24 1146 152 738 Cuts at: 3453 4411 442 Size: 958 1753 Fragments arranged by size: 1753 1146 958 738 416 399 152 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5722 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4382 1239 Size: 3143 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2594 2743 3847 4712 5010 2594 Size: 149 1104 865 298 3306 Fragments arranged by size: 3306 1104 865 298 149 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4385 882 Size: 617 2886 2219 Fragments arranged by size: 2886 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3513 493 Size: 711 2309 2702 Fragments arranged by size: 2702 2309 711 BsaBI GATnn'nnATC Cuts at: 5716 5716 Size: 5722 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2633 2668 3529 3911 Size: 53 83 186 936 1100 35 861 382 Cuts at: 3911 4546 275 Size: 635 1451 Fragments arranged by size: 1451 1100 936 861 635 382 186 83 53 35 BsaWI w'CCGG_w Cuts at: 2115 4155 4553 4827 4974 5385 2115 Size: 2040 398 274 147 411 2452 Fragments arranged by size: 2452 2040 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2036 2066 1178 Size: 30 165 30 633 30 4834 Fragments arranged by size: 4834 633 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5722 BseYI C'CCAG_C Cuts at: 2026 4925 5421 2026 Size: 2899 496 2327 Fragments arranged by size: 2899 2327 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5149 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2116 3172 3933 4961 1540 Size: 275 186 115 1056 761 1028 2301 Fragments arranged by size: 2301 1056 1028 761 275 186 115 BsiHKAI G_wGCw'C Cuts at: 2496 3783 3868 4939 2496 Size: 1287 85 1071 3279 Fragments arranged by size: 3279 1287 1071 85 BsmI GAATG_Cn' Cuts at: 3233 3326 3233 Size: 93 5629 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2138 2347 2496 2803 3041 3783 3868 Size: 399 348 209 149 307 238 742 85 Cuts at: 3868 4939 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 348 307 238 209 149 85 BspEI T'CCGG_A Cuts at: 5385 5385 Size: 5722 BspHI T'CATG_A Cuts at: 3611 5341 3611 Size: 1730 3992 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3077 844 Size: 2233 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3609 1394 Size: 429 1786 3507 Fragments arranged by size: 3507 1786 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2692 2775 4217 4391 65 Size: 1183 1444 83 1442 174 1396 Fragments arranged by size: 1444 1442 1396 1183 174 83 BsrFI r'CCGG_y Cuts at: 1700 1810 4366 4553 1700 Size: 110 2556 187 2869 Fragments arranged by size: 2869 2556 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5722 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5722 BssSI C'ACGA_G Cuts at: 2101 3782 4794 2101 Size: 1681 1012 3029 Fragments arranged by size: 3029 1681 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5722 BstBI TT'CG_AA Cuts at: 4526 4526 Size: 5722 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5722 BstYI r'GATC_y Cuts at: 1972 2154 2848 2860 3052 3419 3804 3821 5273 Size: 182 694 12 192 367 385 17 1452 Cuts at: 5273 5359 5371 5717 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 694 385 367 346 192 182 86 17 12 12 BtgI C'CryG_G Cuts at: 513 1065 2082 513 Size: 552 1017 4153 Fragments arranged by size: 4153 1017 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4983 Fragments arranged by size: 4983 683 56 BtsI GCAGTG_nn' Cuts at: 2977 3238 4032 4052 2977 Size: 261 794 20 4647 Fragments arranged by size: 4647 794 261 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5722 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5687 DraI TTT'AAA Cuts at: 3026 3026 Size: 5722 DraIII CAC_nnn'GTG Cuts at: 2595 2595 Size: 5722 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4729 809 Size: 507 3413 1802 Fragments arranged by size: 3413 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2364 2620 2805 Size: 54 1425 50 275 18 534 256 185 Cuts at: 2805 3169 4058 5687 8 Size: 364 889 1629 43 Fragments arranged by size: 1629 1425 889 534 364 275 256 185 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3169 1537 Size: 275 1357 4090 Fragments arranged by size: 4090 1357 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2406 2538 3652 1353 Size: 1053 132 1114 3423 Fragments arranged by size: 3423 1114 1053 132 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4309 4683 4829 387 Size: 1064 138 2720 374 146 1280 Fragments arranged by size: 2720 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2494 2494 Size: 5722 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2196 3033 3785 4382 5169 5437 1239 Size: 393 564 837 752 597 787 268 1524 Fragments arranged by size: 1524 837 787 752 597 564 393 268 EcoO109I rG'GnC_Cy Cuts at: 2088 2344 2088 Size: 256 5466 EcoRI G'AATT_C Cuts at: 3031 3409 3031 Size: 378 5344 FspI TGC'GCA Cuts at: 4228 4228 Size: 5722 HaeII r_GCGC'y Cuts at: 1678 2629 4869 1678 Size: 951 2240 2531 Fragments arranged by size: 2531 2240 951 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2315 2347 2785 2817 2840 Size: 32 135 32 743 32 438 32 23 Cuts at: 2840 2872 4363 4395 4437 4469 1373 Size: 32 1491 32 42 32 2626 Fragments arranged by size: 2626 1491 743 438 135 42 32 32 32 32 32 32 32 23 HincII GTy'rAC Cuts at: 669 1657 1938 2923 3066 3312 669 Size: 988 281 985 143 246 3079 Fragments arranged by size: 3079 988 985 281 246 143 HindIII A'AGCT_T Cuts at: 748 748 Size: 5722 HpaI GTT'AAC Cuts at: 3312 3312 Size: 5722 KpnI G_GTAC'C Cuts at: 3047 3047 Size: 5722 MfeI C'AATT_G Cuts at: 3321 3321 Size: 5722 MluI A'CGCG_T Cuts at: 5598 5598 Size: 5722 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2705 4836 5020 1305 Size: 1400 2131 184 2007 Fragments arranged by size: 2131 2007 1400 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2366 2622 2807 10 Size: 54 1425 343 534 256 185 2925 Fragments arranged by size: 2925 1425 534 343 256 185 54 MslI CAynn'nnrTG Cuts at: 518 1166 2319 3680 4039 4198 518 Size: 648 1153 1361 359 159 2042 Fragments arranged by size: 2042 1361 1153 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2058 2084 2151 2709 2791 3007 3138 3815 Size: 452 26 67 558 82 216 131 677 Cuts at: 3815 4963 5208 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 558 452 245 216 131 82 67 26 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5170 NdeI CA'TA_TG Cuts at: 387 387 Size: 5722 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5722 NotI GC'GGCC_GC Cuts at: 3169 3169 Size: 5722 NspI r_CATG'y Cuts at: 1132 1229 2471 2706 3080 4625 1132 Size: 97 1242 235 374 1545 2229 Fragments arranged by size: 2229 1545 1242 374 235 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5722 PciI A'CATG_T Cuts at: 4621 4621 Size: 5722 PflMI CCAn_nnn'nTGG Cuts at: 1108 2595 2629 1108 Size: 1487 34 4201 Fragments arranged by size: 4201 1487 34 PmeI GTTT'AAAC Cuts at: 3026 3026 Size: 5722 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3791 3823 5304 5336 3791 Size: 32 1481 32 4177 Fragments arranged by size: 4177 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2088 2088 Size: 5722 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5722 PsiI TTA'TAA Cuts at: 3292 3292 Size: 5722 PspOMI G'GGCC_C Cuts at: 2343 2343 Size: 5722 PstI C_TGCA'G Cuts at: 830 2972 3074 830 Size: 2142 102 3478 Fragments arranged by size: 3478 2142 102 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5722 PvuII CAG'CTG Cuts at: 2151 2791 2151 Size: 640 5082 SacI G_AGCT'C Cuts at: 2496 2496 Size: 5722 SacII CC_GC'GG Cuts at: 2085 2085 Size: 5722 SalI G'TCGA_C Cuts at: 1936 3064 1936 Size: 1128 4594 SanDI GG'GwC_CC Cuts at: 2088 2088 Size: 5722 SbfI CC_TGCA'GG Cuts at: 3074 3074 Size: 5722 ScaI AGT'ACT Cuts at: 3970 5682 3970 Size: 1712 4010 SfcI C'TryA_G Cuts at: 826 1045 2968 3070 4205 4886 5077 826 Size: 219 1923 102 1135 681 191 1471 Fragments arranged by size: 1923 1471 1135 681 219 191 102 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5722 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5722 SmaI CCC'GGG Cuts at: 1872 3049 1872 Size: 1177 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2306 2573 2758 3826 4727 4989 Size: 197 926 363 267 185 1068 901 262 Cuts at: 4989 820 Size: 1553 Fragments arranged by size: 1553 1068 926 901 363 267 262 197 185 SnaBI TAC'GTA Cuts at: 493 3513 493 Size: 3020 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5722 SphI G_CATG'C Cuts at: 1132 2706 3080 1132 Size: 1574 374 3774 Fragments arranged by size: 3774 1574 374 SspI AAT'ATT Cuts at: 5 52 3646 5 Size: 47 3594 2081 Fragments arranged by size: 3594 2081 47 StuI AGG'CCT Cuts at: 1517 3018 1517 Size: 1501 4221 StyI C'CwwG_G Cuts at: 513 1065 2943 3161 513 Size: 552 1878 218 3074 Fragments arranged by size: 3074 1878 552 218 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2496 3749 3766 3919 4104 4549 325 Size: 1255 916 1253 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1253 916 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2738 3968 5680 Size: 274 80 33 51 1443 761 1230 1712 Cuts at: 5680 96 Size: 138 Fragments arranged by size: 1712 1443 1230 761 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2036 2068 5682 5714 2036 Size: 32 3614 32 2044 Fragments arranged by size: 3614 2044 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5722 XbaI T'CTAG_A Cuts at: 3058 3058 Size: 5722 XhoI C'TCGA_G Cuts at: 1943 2306 1943 Size: 363 5359 XmaI C'CCGG_G Cuts at: 1870 3047 1870 Size: 1177 4545 XmnI GAAnn'nnTTC Cuts at: 3851 3851 Size: 5722 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3530 276 Size: 53 83 186 936 1996 2468 Fragments arranged by size: 2468 1996 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SapI SexAI SfiI SfoI SrfI SwaI XcmI