Restriction map for
pFN21AB3413
(Linear) (Six-base) MAPSORT of: KIBB3413.seq Check: 338 from: 1 to: 1017
KIBB3413.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 1017
Size: 191 826
AlwNI CAG_nnn'CTG
Cuts at: 0 624 1017
Size: 624 393
ApaI G_GGCC'C
Cuts at: 0 342 1017
Size: 342 675
AvaI C'yCGr_G
Cuts at: 0 301 609 1017
Size: 301 308 408
Fragments arranged by size:
408 308 301
BamHI G'GATC_C
Cuts at: 0 843 855 1017
Size: 843 12 162
Fragments arranged by size:
843 162 12
BanI G'GyrC_C
Cuts at: 0 473 1017
Size: 473 544
BanII G_rGCy'C
Cuts at: 0 342 491 798 1017
Size: 342 149 307 219
Fragments arranged by size:
342 307 219 149
Bme1580I G_kGCm'C
Cuts at: 0 133 342 1017
Size: 133 209 675
Fragments arranged by size:
675 209 133
BmrI ACTGGGnnnn_n'
Cuts at: 0 558 710 1017
Size: 558 152 307
Fragments arranged by size:
558 307 152
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 738 1017
Size: 589 149 279
Fragments arranged by size:
589 279 149
BsaHI Gr'CG_yC
Cuts at: 0 628 663 1017
Size: 628 35 354
Fragments arranged by size:
628 354 35
BsaWI w'CCGG_w
Cuts at: 0 110 1017
Size: 110 907
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 31 61 1017
Size: 31 30 956
Fragments arranged by size:
956 31 30
BseYI C'CCAG_C
Cuts at: 0 21 1017
Size: 21 996
BsiEI CG_ry'CG
Cuts at: 0 111 1017
Size: 111 906
BsiHKAI G_wGCw'C
Cuts at: 0 491 1017
Size: 491 526
Bsp1286I G_dGCh'C
Cuts at: 0 133 342 491 798 1017
Size: 133 209 149 307 219
Fragments arranged by size:
307 219 209 149 133
BsrDI GCAATG_nn'
Cuts at: 0 687 770 1017
Size: 687 83 247
Fragments arranged by size:
687 247 83
BssSI C'ACGA_G
Cuts at: 0 96 1017
Size: 96 921
BstYI r'GATC_y
Cuts at: 0 149 843 855 1017
Size: 149 694 12 162
Fragments arranged by size:
694 162 149 12
BtgI C'CryG_G
Cuts at: 0 77 1017
Size: 77 940
BtsI GCAGTG_nn'
Cuts at: 0 972 1017
Size: 972 45
DraIII CAC_nnn'GTG
Cuts at: 0 590 1017
Size: 590 427
EaeI y'GGCC_r
Cuts at: 0 359 615 800 1017
Size: 359 256 185 217
Fragments arranged by size:
359 256 217 185
EarI CTCTTCn'nnn_
Cuts at: 0 401 533 1017
Size: 401 132 484
Fragments arranged by size:
484 401 132
EcoICRI GAG'CTC
Cuts at: 0 489 1017
Size: 489 528
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 1017
Size: 191 826
EcoO109I rG'GnC_Cy
Cuts at: 0 83 339 1017
Size: 83 256 678
Fragments arranged by size:
678 256 83
HaeII r_GCGC'y
Cuts at: 0 624 1017
Size: 624 393
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 780 812 835 867 1017
Size: 310 32 438 32 23 32 150
Fragments arranged by size:
438 310 150 32 32 32 23
HincII GTy'rAC
Cuts at: 0 918 1017
Size: 918 99
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 1017
Size: 700 317
MscI TGG'CCA
Cuts at: 0 361 617 802 1017
Size: 361 256 185 215
Fragments arranged by size:
361 256 215 185
MslI CAynn'nnrTG
Cuts at: 0 314 1017
Size: 314 703
MspA1I CmG'CkG
Cuts at: 0 53 79 146 704 786 1002 1017
Size: 53 26 67 558 82 216 15
Fragments arranged by size:
558 216 82 67 53 26 15
NspI r_CATG'y
Cuts at: 0 466 701 1017
Size: 466 235 316
Fragments arranged by size:
466 316 235
PflMI CCAn_nnn'nTGG
Cuts at: 0 590 624 1017
Size: 590 34 393
Fragments arranged by size:
590 393 34
PpuMI rG'GwC_Cy
Cuts at: 0 83 1017
Size: 83 934
PspOMI G'GGCC_C
Cuts at: 0 338 1017
Size: 338 679
PstI C_TGCA'G
Cuts at: 0 967 1017
Size: 967 50
PvuII CAG'CTG
Cuts at: 0 146 786 1017
Size: 146 640 231
Fragments arranged by size:
640 231 146
SacI G_AGCT'C
Cuts at: 0 491 1017
Size: 491 526
SacII CC_GC'GG
Cuts at: 0 80 1017
Size: 80 937
SanDI GG'GwC_CC
Cuts at: 0 83 1017
Size: 83 934
SfcI C'TryA_G
Cuts at: 0 963 1017
Size: 963 54
SmlI C'TyrA_G
Cuts at: 0 301 568 753 1017
Size: 301 267 185 264
Fragments arranged by size:
301 267 264 185
SphI G_CATG'C
Cuts at: 0 701 1017
Size: 701 316
StuI AGG'CCT
Cuts at: 0 1013 1017
Size: 1013 4
StyI C'CwwG_G
Cuts at: 0 938 1017
Size: 938 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 491 1017
Size: 491 526
TatI w'GTAC_w
Cuts at: 0 733 1017
Size: 733 284
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 31 63 1017
Size: 31 32 954
Fragments arranged by size:
954 32 31
XhoI C'TCGA_G
Cuts at: 0 301 1017
Size: 301 716
Enzymes that do cut:
AcuI AlwNI ApaI AvaI BamHI BanI BanII Bme1580I
BmrI BpuEI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
Bsp1286I BsrDI BssSI BstYI BtgI BtsI DraIII EaeI
EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII MmeI
MscI MslI MspA1I NspI PflMI PpuMI PspOMI PstI
PvuII SacI SacII SanDI SfcI SmlI SphI StuI
StyI TaqII TatI TstI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI
BplI BpmI Bpu10I BsaI BsaAI BsaBI BseRI BsgI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DrdI EagI EciI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI Tth111I XbaI XcmI XmaI XmnI ZraI