Restriction map for
pFN21AB3406
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BglI BlnI
BmyI BpuAI BsaBI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I Cac8I CfoI
Cfr10I ClaI CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HpaI HpaII KasI
KpnI KspI MamI MfeI MluI
MroI MspI MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SphI Sse8387I SspBI SspI StuI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 24 CGCG CG^CG
AfaI 802 GTAC GT^AC
AflII 91 CTTAAG C^TTAAG
AlwNI 340 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 565 ATTAAT AT^TAAT
AsnI 565 ATTAAT AT^TAAT
BalI 176 TGGCCA TGG^CCA
BfaI 871 CTAG C^TAG
BfrI 91 CTTAAG C^TTAAG
BglII 851 AGATCT A^GATCT
BlpI 106 GCTAAGC GC^T[ACGT]AGC
BpmI 939 CTCCAG CTGGAG(16/14)
Bpu1102I 106 GCTAAGC GC^T[ACGT]AGC
BsaAI 662 TACGTA [CT]AC^GT[GA]
BsaI 1018 GGTCTC GGTCTC(1/5)
BsmAI 1019 GTCTC GTCTC(1/5)
BsrDI 405 CATTGC GCAATG(2/0)
BstUI 24 CGCG CG^CG
CelII 106 GCTAAGC GC^T[ACGT]AGC
DraI 997 TTTAAA TTT^AAA
EaeI 176 TGGCCA [CT]^GGCC[GA]
EarI 615 GAAGAG CTCTTC(1/4)
EcoRI 283 GAATTC G^AATTC
EcoRV 775 GATATC GAT^ATC
EcoT14I 374 CCAAGG C^C[AT][AT]GG
HincII 231 GTCAAC GT[CT]^[GA]AC
HindII 231 GTCAAC GT[CT]^[GA]AC
HindIII 109 AAGCTT A^AGCTT
Ksp632I 615 GAAGAG CTCTTC(1/4)
MaeI 871 CTAG C^TAG
MluNI 176 TGGCCA TGG^CCA
MscI 176 TGGCCA TGG^CCA
MvnI 24 CGCG CG^CG
NlaIII 966 CATG CATG^
PshBI 565 ATTAAT AT^TAAT
PvuII 677 CAGCTG CAG^CTG
RsaI 802 GTAC GT^AC
SapI 615 GAAGAGC GCTCTTC(1/4)
SfcI 674 CTACAG C^T[GA][CT]AG
SmlI 91 CTTAAG C^T[CT][GA]AG
SnaBI 662 TACGTA TAC^GTA
SpeI 870 ACTAGT A^CTAGT
StyI 374 CCAAGG C^C[AT][AT]GG
SwaI 996 ATTTAAAT ATTT^AAAT
Tsp45I 655 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AciI 822 CCGC CCGC(-3/-1)
25 GCGG
AlwI 336 GATCC GGATC(4/5)
348 GGATC
AvaII 154 GGTCC G^G[AT]CC
944 GGACC
BsaJI 354 CCCAGG C^C[ACGT][ACGT]GG
374 CCAAGG
BsmFI 465 GGGAC GGGAC(10/14)
857 GGGAC
BsmI 506 GCATTC GAATGC(1/-1)
620 GCATTC
BsrI 900 CCAGT ACTGG(1/-1)
922 CCAGT
HphI 187 GGTGA GGTGA(8/7)
819 TCACC
MaeII 663 ACGT A^CGT
1063 ACGT
MslI 405 CATTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
690 CATTGATGTG
MspA1I 677 CAGCTG C[AC]G^C[GT]G
822 CCGCTG
PleI 51 GACTC GAGTC(4/5)
657 GACTC
TaqI 117 TCGA T^CGA
195 TCGA
TfiI 639 GATTC G^A[AT]TC
972 GATTC
TthHB8I 117 TCGA T^CGA
195 TCGA
XcmI 761 CCATTGAGGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
941 CCAGGACCACAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AcsI AluI ApoI BanI BbvI
BsiYI BslI BssKI BstF5I BstNI
BstYI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MaeIII MboI MboII MflI MnlI
MseI MvaI MwoI NdeII NlaIV
PflMI Sau3AI Sau96I ScrFI SfaNI
Tru9I TseI Tsp509I TspRI Van91I
XhoII