Restriction map for
pFN21AB3406
(Linear) (Six-base) MAPSORT of: KIBB3406.seq Check: 8259 from: 1 to: 1101
KIBB3406.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AflII C'TTAA_G
Cuts at: 0 91 1101
Size: 91 1010
AlwNI CAG_nnn'CTG
Cuts at: 0 345 1101
Size: 345 756
ApoI r'AATT_y
Cuts at: 0 283 828 878 994 1101
Size: 283 545 50 116 107
Fragments arranged by size:
545 283 116 107 50
AseI AT'TA_AT
Cuts at: 0 566 1101
Size: 566 535
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 312 345 1101
Size: 312 33 756
Fragments arranged by size:
756 312 33
BanI G'GyrC_C
Cuts at: 0 328 370 789 1101
Size: 328 42 419 312
Fragments arranged by size:
419 328 312 42
BglII A'GATC_T
Cuts at: 0 851 1101
Size: 851 250
BlpI GC'TnA_GC
Cuts at: 0 107 1101
Size: 107 994
BmrI ACTGGGnnnn_n'
Cuts at: 0 916 1101
Size: 916 185
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 924 1101
Size: 924 177
Bpu10I CC'TnA_GC
Cuts at: 0 12 1101
Size: 12 1089
BsaI GGTCTCn'nnnn_
Cuts at: 0 1024 1101
Size: 1024 77
BsaAI yAC'GTr
Cuts at: 0 664 1101
Size: 664 437
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 742 772 1101
Size: 742 30 329
Fragments arranged by size:
742 329 30
BsmI GAATG_Cn'
Cuts at: 0 506 620 1101
Size: 506 114 481
Fragments arranged by size:
506 481 114
BsrDI GCAATG_nn'
Cuts at: 0 404 1101
Size: 404 697
BstYI r'GATC_y
Cuts at: 0 335 348 851 1101
Size: 335 13 503 250
Fragments arranged by size:
503 335 250 13
BtsI GCAGTG_nn'
Cuts at: 0 235 416 1101
Size: 235 181 685
Fragments arranged by size:
685 235 181
DraI TTT'AAA
Cuts at: 0 999 1101
Size: 999 102
EaeI y'GGCC_r
Cuts at: 0 176 1101
Size: 176 925
EarI CTCTTCn'nnn_
Cuts at: 0 610 1101
Size: 610 491
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 924 1101
Size: 924 177
EcoRI G'AATT_C
Cuts at: 0 283 1101
Size: 283 818
EcoRV GAT'ATC
Cuts at: 0 777 1101
Size: 777 324
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 704 736 742 774 901 933 1101
Size: 704 32 6 32 127 32 168
Fragments arranged by size:
704 168 127 32 32 32 6
HincII GTy'rAC
Cuts at: 0 233 1101
Size: 233 868
HindIII A'AGCT_T
Cuts at: 0 109 1101
Size: 109 992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1046 1101
Size: 1046 55
MscI TGG'CCA
Cuts at: 0 178 1101
Size: 178 923
MslI CAynn'nnrTG
Cuts at: 0 409 694 1101
Size: 409 285 407
Fragments arranged by size:
409 407 285
MspA1I CmG'CkG
Cuts at: 0 679 824 1101
Size: 679 145 277
Fragments arranged by size:
679 277 145
PflMI CCAn_nnn'nTGG
Cuts at: 0 139 345 1065 1101
Size: 139 206 720 36
Fragments arranged by size:
720 206 139 36
PfoI T'CCnGG_A
Cuts at: 0 156 344 940 1101
Size: 156 188 596 161
Fragments arranged by size:
596 188 161 156
PvuII CAG'CTG
Cuts at: 0 679 1101
Size: 679 422
SapI GCTCTTCn'nnn_
Cuts at: 0 610 1101
Size: 610 491
SfcI C'TryA_G
Cuts at: 0 674 1101
Size: 674 427
SmlI C'TyrA_G
Cuts at: 0 91 1101
Size: 91 1010
SnaBI TAC'GTA
Cuts at: 0 664 1101
Size: 664 437
SpeI A'CTAG_T
Cuts at: 0 870 1101
Size: 870 231
StyI C'CwwG_G
Cuts at: 0 374 1101
Size: 374 727
SwaI ATTT'AAAT
Cuts at: 0 999 1101
Size: 999 102
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 768 948 1101
Size: 768 180 153
Fragments arranged by size:
768 180 153
Enzymes that do cut:
AflII AlwNI ApoI AseI BaeI BanI BglII BlpI
BmrI BpmI Bpu10I BsaI BsaAI BsaXI BsmI BsrDI
BstYI BtsI DraI EaeI EarI Eco57MI EcoRI EcoRV
Hin4I HincII HindIII MmeI MscI MslI MspA1I PflMI
PfoI PvuII SapI SfcI SmlI SnaBI SpeI StyI
SwaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AsiSI AvaI AvrII BamHI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI Bme1580I BmgBI BmtI
BplI BpuEI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoO109I FalI FseI
FspI FspAI HaeII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SphI SrfI SspI
StuI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI