(Circular) (Six-base) MAPSORT of: pFN21AB3372.seq Check: 7410 from: 1 to: 6040 pFN21AB3372 6040 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3850 278 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2195 2682 3383 1937 Size: 258 487 701 4594 Fragments arranged by size: 4594 701 487 258 Acc65I G'GTAC_C Cuts at: 3361 3361 Size: 6040 AclI AA'CG_TT Cuts at: 1563 4167 4540 1563 Size: 2604 373 3063 Fragments arranged by size: 3063 2604 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2177 2896 4103 5487 5755 1632 Size: 545 719 1207 1384 268 1917 Fragments arranged by size: 1917 1384 1207 719 545 268 AflII C'TTAA_G Cuts at: 820 1017 3061 820 Size: 197 2044 3799 Fragments arranged by size: 3799 2044 197 AflIII A'CryG_T Cuts at: 1203 2451 4939 5916 1203 Size: 1248 2488 977 1327 Fragments arranged by size: 2488 1327 1248 977 AgeI A'CCGG_T Cuts at: 4871 4871 Size: 6040 AhdI GACnn_n'nnGTC Cuts at: 2191 4769 2191 Size: 2578 3462 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6040 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2604 1184 Size: 1420 4620 AlwNI CAG_nnn'CTG Cuts at: 2470 3783 5355 2470 Size: 1313 1572 3155 Fragments arranged by size: 3155 1572 1313 ApaLI G'TGCA_C Cuts at: 4097 5253 4097 Size: 1156 4884 ApoI r'AATT_y Cuts at: 1493 2052 3083 3349 3575 3727 5836 1493 Size: 559 1031 266 226 152 2109 1697 Fragments arranged by size: 2109 1697 1031 559 266 226 152 AseI AT'TA_AT Cuts at: 160 1032 4594 160 Size: 872 3562 1606 Fragments arranged by size: 3562 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 AvaI C'yCGr_G Cuts at: 1870 1943 2521 3365 1870 Size: 73 578 844 4545 Fragments arranged by size: 4545 844 578 73 BamHI G'GATC_C Cuts at: 3370 3737 3370 Size: 367 5673 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2237 3149 3361 4816 618 Size: 325 217 627 450 912 212 1455 1842 Fragments arranged by size: 1842 1455 912 627 450 325 217 212 BanII G_rGCy'C Cuts at: 1391 2361 2910 3144 3359 1391 Size: 970 549 234 215 4072 Fragments arranged by size: 4072 970 549 234 215 BbeI G_GCGC'C Cuts at: 2241 2241 Size: 6040 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5069 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4231 4265 1903 Size: 34 2294 34 3678 Fragments arranged by size: 3678 2294 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2075 2865 3932 5142 2075 Size: 790 1067 1210 2973 Fragments arranged by size: 2973 1210 1067 790 BclI T'GATC_A Cuts at: 1548 1548 Size: 6040 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2174 2562 4651 Size: 107 122 71 631 605 502 388 2089 Cuts at: 4651 136 Size: 1525 Fragments arranged by size: 2089 1525 631 605 502 388 122 107 71 BglII A'GATC_T Cuts at: 6035 6035 Size: 6040 BlpI GC'TnA_GC Cuts at: 3457 3457 Size: 6040 Bme1580I G_kGCm'C Cuts at: 1790 4101 5257 1790 Size: 2311 1156 2573 Fragments arranged by size: 2573 2311 1156 BmgBI CAC'GTC Cuts at: 1318 2893 1318 Size: 1575 4465 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3233 3771 4729 Size: 416 53 83 399 24 1816 538 958 Cuts at: 4729 442 Size: 1753 Fragments arranged by size: 1816 1753 958 538 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6040 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2536 2536 Size: 6040 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2300 3017 3174 3249 4700 1239 Size: 1061 717 157 75 1451 2579 Fragments arranged by size: 2579 1451 1061 717 157 75 Bpu10I CC'TnA_GC Cuts at: 2845 2922 3138 2845 Size: 77 216 5747 Fragments arranged by size: 5747 216 77 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2843 4165 5030 5328 2843 Size: 1322 865 298 3555 Fragments arranged by size: 3555 1322 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4703 882 Size: 617 3204 2219 Fragments arranged by size: 3204 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2454 3831 493 Size: 711 1250 1377 2702 Fragments arranged by size: 2702 1377 1250 711 BsaBI GATnn'nnATC Cuts at: 2150 6034 2150 Size: 3884 2156 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2238 2990 3847 4229 Size: 53 83 186 936 705 752 857 382 Cuts at: 4229 4864 275 Size: 635 1451 Fragments arranged by size: 1451 936 857 752 705 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2442 4473 4871 5145 5292 5703 2442 Size: 2031 398 274 147 411 2779 Fragments arranged by size: 2779 2031 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2705 2735 1178 Size: 30 165 30 1302 30 4483 Fragments arranged by size: 4483 1302 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2952 1186 Size: 1766 4274 BseYI C'CCAG_C Cuts at: 2551 5243 5739 2551 Size: 2692 496 2852 Fragments arranged by size: 2852 2692 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2078 2119 2693 2941 1163 Size: 573 342 41 574 248 4262 Fragments arranged by size: 4262 574 573 342 248 41 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2128 3490 4251 5279 1540 Size: 275 186 127 1362 761 1028 2301 Fragments arranged by size: 2301 1362 1028 761 275 186 127 BsiHKAI G_wGCw'C Cuts at: 2361 3144 3327 4101 4186 5257 2361 Size: 783 183 774 85 1071 3144 Fragments arranged by size: 3144 1071 783 774 183 85 BsmI GAATG_Cn' Cuts at: 2798 3551 3644 2798 Size: 753 93 5194 Fragments arranged by size: 5194 753 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2540 2725 2540 Size: 185 5855 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2361 2910 3144 3327 3359 4101 4186 Size: 399 571 549 234 183 32 742 85 Cuts at: 4186 5257 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 571 549 399 234 183 85 32 BspEI T'CCGG_A Cuts at: 5703 5703 Size: 6040 BspHI T'CATG_A Cuts at: 2079 3929 5659 2079 Size: 1850 1730 2460 Fragments arranged by size: 2460 1850 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3395 844 Size: 2551 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2528 2659 3927 1394 Size: 429 705 131 1268 3507 Fragments arranged by size: 3507 1268 705 429 131 BsrDI GCAATG_nn' Cuts at: 65 1248 4535 4709 65 Size: 1183 3287 174 1396 Fragments arranged by size: 3287 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2351 4684 4871 1700 Size: 110 541 2333 187 2869 Fragments arranged by size: 2869 2333 541 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6040 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6040 BssSI C'ACGA_G Cuts at: 2210 4100 5112 2210 Size: 1890 1012 3138 Fragments arranged by size: 3138 1890 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2698 794 Size: 1904 4136 BstBI TT'CG_AA Cuts at: 4844 4844 Size: 6040 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6040 BstYI r'GATC_y Cuts at: 1972 2505 3024 3370 3737 4122 4139 5591 5677 Size: 533 519 346 367 385 17 1452 86 Cuts at: 5677 5689 6035 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 533 519 385 367 346 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2232 2394 2539 2818 3210 513 Size: 552 939 228 162 145 279 392 3343 Fragments arranged by size: 3343 939 552 392 279 228 162 145 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5301 Fragments arranged by size: 5301 683 56 BtsI GCAGTG_nn' Cuts at: 2326 2468 2728 2967 3556 4350 4370 2326 Size: 142 260 239 589 794 20 3996 Fragments arranged by size: 3996 794 589 260 239 142 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6040 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6005 DraI TTT'AAA Cuts at: 3344 3344 Size: 6040 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5047 809 Size: 507 3731 1802 Fragments arranged by size: 3731 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2125 2166 3487 Size: 54 1425 50 275 18 295 41 1321 Cuts at: 3487 4376 6005 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1321 889 295 275 54 50 43 41 18 EagI C'GGCC_G Cuts at: 1537 1812 2125 3487 1537 Size: 275 313 1362 4090 Fragments arranged by size: 4090 1362 313 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2394 3970 1353 Size: 1041 1576 3423 Fragments arranged by size: 3423 1576 1041 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2057 3017 3156 4627 5001 5147 Size: 1064 138 468 960 139 1471 374 146 Cuts at: 5147 387 Size: 1280 Fragments arranged by size: 1471 1280 1064 960 468 374 146 139 138 EcoICRI GAG'CTC Cuts at: 2359 3142 2359 Size: 783 5257 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2177 2300 2896 3017 3174 3249 4103 Size: 393 545 123 596 121 157 75 854 Cuts at: 4103 4700 5487 5755 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 854 787 597 596 545 393 268 157 123 121 75 EcoNI CCTnn'n_nnAGG Cuts at: 2861 2861 Size: 6040 EcoO109I rG'GnC_Cy Cuts at: 2161 2275 2293 2161 Size: 114 18 5908 Fragments arranged by size: 5908 114 18 EcoRI G'AATT_C Cuts at: 3083 3349 3727 3083 Size: 266 378 5396 Fragments arranged by size: 5396 378 266 EcoRV GAT'ATC Cuts at: 3164 3164 Size: 6040 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3103 3103 Size: 6040 FspI TGC'GCA Cuts at: 4546 4546 Size: 6040 HaeII r_GCGC'y Cuts at: 1678 2117 2241 3104 3284 5187 1678 Size: 439 124 863 180 1903 2531 Fragments arranged by size: 2531 1903 863 439 180 124 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2177 2209 2604 2636 2705 Size: 32 135 32 605 32 395 32 69 Cuts at: 2705 2737 2975 3007 4681 4713 4755 4787 1373 Size: 32 238 32 1674 32 42 32 2626 Fragments arranged by size: 2626 1674 605 395 238 135 69 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2196 3384 3630 669 Size: 988 281 258 1188 246 3079 Fragments arranged by size: 3079 1188 988 281 258 246 HindIII A'AGCT_T Cuts at: 748 3058 748 Size: 2310 3730 HpaI GTT'AAC Cuts at: 3630 3630 Size: 6040 KasI G'GCGC_C Cuts at: 2237 2237 Size: 6040 KpnI G_GTAC'C Cuts at: 3365 3365 Size: 6040 MfeI C'AATT_G Cuts at: 3639 3639 Size: 6040 MluI A'CGCG_T Cuts at: 5916 5916 Size: 6040 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5154 5338 1305 Size: 3849 184 2007 Fragments arranged by size: 3849 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2168 10 Size: 54 1425 343 336 3882 Fragments arranged by size: 3882 1425 343 336 54 MslI CAynn'nnrTG Cuts at: 518 1166 3239 3998 4357 4516 518 Size: 648 2073 759 359 159 2042 Fragments arranged by size: 2073 2042 759 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2408 3014 3456 4133 5281 5526 1606 Size: 802 606 442 677 1148 245 2120 Fragments arranged by size: 2120 1148 802 677 606 442 245 NaeI GCC'GGC Cuts at: 2353 2353 Size: 6040 NarI GG'CG_CC Cuts at: 2238 2238 Size: 6040 NcoI C'CATG_G Cuts at: 513 1065 2004 3210 513 Size: 552 939 1206 3343 Fragments arranged by size: 3343 1206 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6040 NgoMIV G'CCGG_C Cuts at: 2351 2351 Size: 6040 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6040 NotI GC'GGCC_GC Cuts at: 3487 3487 Size: 6040 NspI r_CATG'y Cuts at: 1132 1229 3398 4943 1132 Size: 97 2169 1545 2229 Fragments arranged by size: 2229 2169 1545 97 PasI CC'CwG_GG Cuts at: 1348 2665 1348 Size: 1317 4723 PciI A'CATG_T Cuts at: 4939 4939 Size: 6040 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6040 PfoI T'CCnGG_A Cuts at: 3190 3190 Size: 6040 PmeI GTTT'AAAC Cuts at: 3344 3344 Size: 6040 PmlI CAC'GTG Cuts at: 2454 2454 Size: 6040 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4109 4141 5622 5654 4109 Size: 32 1481 32 4495 Fragments arranged by size: 4495 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2161 2275 2161 Size: 114 5926 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6040 PsiI TTA'TAA Cuts at: 3610 3610 Size: 6040 PstI C_TGCA'G Cuts at: 830 2392 2694 3208 3392 830 Size: 1562 302 514 184 3478 Fragments arranged by size: 3478 1562 514 302 184 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6040 PvuII CAG'CTG Cuts at: 3014 3014 Size: 6040 SacI G_AGCT'C Cuts at: 2361 3144 2361 Size: 783 5257 SalI G'TCGA_C Cuts at: 1936 2194 3382 1936 Size: 258 1188 4594 Fragments arranged by size: 4594 1188 258 SbfI CC_TGCA'GG Cuts at: 2694 3392 2694 Size: 698 5342 ScaI AGT'ACT Cuts at: 4288 6000 4288 Size: 1712 4328 SfcI C'TryA_G Cuts at: 826 1045 2388 2690 3204 3388 4523 5204 5395 Size: 219 1343 302 514 184 1135 681 191 Cuts at: 5395 826 Size: 1471 Fragments arranged by size: 1471 1343 1135 681 514 302 219 191 184 SfiI GGCCn_nnn'nGGCC Cuts at: 2174 2174 Size: 6040 SfoI GGC'GCC Cuts at: 2239 2239 Size: 6040 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6040 SmaI CCC'GGG Cuts at: 1872 3367 1872 Size: 1495 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2858 3061 4144 5045 5307 820 Size: 197 926 915 203 1083 901 262 1553 Fragments arranged by size: 1553 1083 926 915 901 262 203 197 SnaBI TAC'GTA Cuts at: 493 3831 493 Size: 3338 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6040 SphI G_CATG'C Cuts at: 1132 3398 1132 Size: 2266 3774 SspI AAT'ATT Cuts at: 5 52 3964 5 Size: 47 3912 2081 Fragments arranged by size: 3912 2081 47 StuI AGG'CCT Cuts at: 1517 2606 1517 Size: 1089 4951 StyI C'CwwG_G Cuts at: 513 1065 2004 2601 3104 3210 3479 513 Size: 552 939 597 503 106 269 3074 Fragments arranged by size: 3074 939 597 552 503 269 106 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3170 4067 4084 4237 4422 4867 325 Size: 1255 1590 897 17 153 185 445 1498 Fragments arranged by size: 1590 1498 1255 897 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4286 5998 96 Size: 274 80 33 51 1443 2309 1712 138 Fragments arranged by size: 2309 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2305 2337 2355 2387 6000 6032 2305 Size: 32 18 32 3613 32 2313 Fragments arranged by size: 3613 2313 32 32 32 18 Tth111I GACn'n_nGTC Cuts at: 1316 2717 1316 Size: 1401 4639 XbaI T'CTAG_A Cuts at: 3376 3376 Size: 6040 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2275 2011 Size: 264 5776 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6040 XmaI C'CCGG_G Cuts at: 1870 3365 1870 Size: 1495 4545 XmnI GAAnn'nnTTC Cuts at: 4169 4169 Size: 6040 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3848 276 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII FseI FspAI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SrfI SwaI