(Circular) (Six-base) MAPSORT of: pFN21AB3233.seq Check: 6849 from: 1 to: 7045 pFN21AB3233 7045 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4855 278 Size: 53 83 186 936 3319 2468 Fragments arranged by size: 3319 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4388 1937 Size: 2451 4594 Acc65I G'GTAC_C Cuts at: 4366 4366 Size: 7045 AclI AA'CG_TT Cuts at: 1563 5172 5545 1563 Size: 3609 373 3063 Fragments arranged by size: 3609 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2219 3137 3601 4202 4272 5108 6492 6760 Size: 587 918 464 601 70 836 1384 268 Cuts at: 6760 1632 Size: 1917 Fragments arranged by size: 1917 1384 918 836 601 587 464 268 70 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6848 AflIII A'CryG_T Cuts at: 1203 5944 6921 1203 Size: 4741 977 1327 Fragments arranged by size: 4741 1327 977 AgeI A'CCGG_T Cuts at: 2135 5876 2135 Size: 3741 3304 AhdI GACnn_n'nnGTC Cuts at: 5774 5774 Size: 7045 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7045 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3317 1184 Size: 2133 4912 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2749 2781 2749 Size: 32 7013 AlwNI CAG_nnn'CTG Cuts at: 3215 3686 4175 4788 6360 3215 Size: 471 489 613 1572 3900 Fragments arranged by size: 3900 1572 613 489 471 ApaLI G'TGCA_C Cuts at: 5102 6258 5102 Size: 1156 5889 ApoI r'AATT_y Cuts at: 1493 2934 3101 4024 4354 4580 4732 6841 1493 Size: 1441 167 923 330 226 152 2109 1697 Fragments arranged by size: 2109 1697 1441 923 330 226 167 152 AseI AT'TA_AT Cuts at: 160 1032 5599 160 Size: 872 4567 1606 Fragments arranged by size: 4567 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7045 AvaI C'yCGr_G Cuts at: 1870 1943 2877 4370 1870 Size: 73 934 1493 4545 Fragments arranged by size: 4545 1493 934 73 BamHI G'GATC_C Cuts at: 4375 4742 4375 Size: 367 6678 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2138 2516 3689 4043 4366 Size: 325 217 627 351 378 1173 354 323 Cuts at: 4366 5821 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1173 627 378 354 351 325 323 217 BanII G_rGCy'C Cuts at: 1391 3235 3558 3801 4007 4156 4364 1391 Size: 1844 323 243 206 149 208 4072 Fragments arranged by size: 4072 1844 323 243 208 206 149 BbeI G_GCGC'C Cuts at: 4047 4047 Size: 7045 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2140 2841 4153 928 Size: 971 241 701 1312 3820 Fragments arranged by size: 3820 1312 971 701 241 BbvCI CC'TCA_GC Cuts at: 2793 2793 Size: 7045 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5236 5270 1903 Size: 34 3299 34 3678 Fragments arranged by size: 3678 3299 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2636 4937 6147 2636 Size: 2301 1210 3534 Fragments arranged by size: 3534 2301 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7045 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3393 5656 136 Size: 107 122 71 631 605 1721 2263 1525 Fragments arranged by size: 2263 1721 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7040 7040 Size: 7045 BlpI GC'TnA_GC Cuts at: 4176 4462 4176 Size: 286 6759 Bme1580I G_kGCm'C Cuts at: 1790 2143 2266 5106 6262 1790 Size: 353 123 2840 1156 2573 Fragments arranged by size: 2840 2573 1156 353 123 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7045 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2382 2692 2728 Size: 416 53 83 399 24 965 310 36 Cuts at: 2728 3157 4776 5734 442 Size: 429 1619 958 1753 Fragments arranged by size: 1753 1619 965 958 429 416 399 310 83 53 36 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7045 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2405 3382 4101 4325 5705 1239 Size: 1166 977 719 224 1380 2579 Fragments arranged by size: 2579 1380 1166 977 719 224 Bpu10I CC'TnA_GC Cuts at: 2793 2793 Size: 7045 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5170 6035 6333 5170 Size: 865 298 5882 Fragments arranged by size: 5882 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3807 5708 882 Size: 617 2308 1901 2219 Fragments arranged by size: 2308 2219 1901 617 BsaAI yAC'GTr Cuts at: 493 1204 4836 493 Size: 711 3632 2702 Fragments arranged by size: 3632 2702 711 BsaBI GATnn'nnATC Cuts at: 7039 7039 Size: 7045 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2915 4044 4852 5234 Size: 53 83 186 936 1382 1129 808 382 Cuts at: 5234 5869 275 Size: 635 1451 Fragments arranged by size: 1451 1382 1129 936 808 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2135 5478 5876 6150 6297 6708 2135 Size: 3343 398 274 147 411 2472 Fragments arranged by size: 3343 2472 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2235 2265 1178 Size: 30 165 30 832 30 5958 Fragments arranged by size: 5958 832 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2462 3809 3956 1186 Size: 1276 1347 147 4275 Fragments arranged by size: 4275 1347 1276 147 BseYI C'CCAG_C Cuts at: 2239 2998 3767 6248 6744 2239 Size: 759 769 2481 496 2540 Fragments arranged by size: 2540 2481 769 759 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2921 3637 1163 Size: 573 1185 716 4571 Fragments arranged by size: 4571 1185 716 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4341 4495 5256 6284 1540 Size: 275 186 2340 154 761 1028 2301 Fragments arranged by size: 2340 2301 1028 761 275 186 154 BsiHKAI G_wGCw'C Cuts at: 2022 3306 3558 4311 5106 5191 6262 2022 Size: 1284 252 753 795 85 1071 2805 Fragments arranged by size: 2805 1284 1071 795 753 252 85 BsmI GAATG_Cn' Cuts at: 2101 3256 4556 4649 2101 Size: 1155 1300 93 4497 Fragments arranged by size: 4497 1300 1155 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2777 2777 Size: 7045 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2143 2266 3235 3306 3558 3801 Size: 399 232 121 123 969 71 252 243 Cuts at: 3801 4007 4156 4311 4364 5106 5191 6262 1391 Size: 206 149 155 53 742 85 1071 2174 Fragments arranged by size: 2174 1071 969 742 399 252 243 232 206 155 149 123 121 85 71 53 BspEI T'CCGG_A Cuts at: 6708 6708 Size: 7045 BspHI T'CATG_A Cuts at: 3138 4934 6664 3138 Size: 1796 1730 3519 Fragments arranged by size: 3519 1796 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2420 3825 4400 844 Size: 1576 1405 575 3489 Fragments arranged by size: 3489 1576 1405 575 BsrBI CCG'CTC Cuts at: 1394 1823 4932 1394 Size: 429 3109 3507 Fragments arranged by size: 3507 3109 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2055 3904 5540 5714 65 Size: 1183 807 1849 1636 174 1396 Fragments arranged by size: 1849 1636 1396 1183 807 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2135 5689 5876 1700 Size: 110 325 3554 187 2869 Fragments arranged by size: 3554 2869 325 187 110 BsrGI T'GTAC_A Cuts at: 96 3156 96 Size: 3060 3985 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7045 BssSI C'ACGA_G Cuts at: 5105 6117 5105 Size: 1012 6033 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7045 BstBI TT'CG_AA Cuts at: 5849 5849 Size: 7045 BstEII G'GTnAC_C Cuts at: 1184 3023 3064 1184 Size: 1839 41 5165 Fragments arranged by size: 5165 1839 41 BstXI CCAn_nnnn'nTGG Cuts at: 2038 3088 4039 2038 Size: 1050 951 5044 Fragments arranged by size: 5044 1050 951 BstYI r'GATC_y Cuts at: 1972 4101 4375 4742 5127 5144 6596 6682 6694 Size: 2129 274 367 385 17 1452 86 12 Cuts at: 6694 7040 1972 Size: 346 1977 Fragments arranged by size: 2129 1977 1452 385 367 346 274 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2761 3087 3750 513 Size: 552 1696 326 663 3808 Fragments arranged by size: 3808 1696 663 552 326 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6306 Fragments arranged by size: 6306 683 56 BtsI GCAGTG_nn' Cuts at: 2586 2970 4561 5355 5375 2586 Size: 384 1591 794 20 4256 Fragments arranged by size: 4256 1591 794 384 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7045 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7010 DraI TTT'AAA Cuts at: 4349 4349 Size: 7045 DraIII CAC_nnn'GTG Cuts at: 3211 3211 Size: 7045 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6052 809 Size: 507 4736 1802 Fragments arranged by size: 4736 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3333 3385 4338 Size: 54 1425 50 275 18 1503 52 953 Cuts at: 4338 4492 5381 7010 8 Size: 154 889 1629 43 Fragments arranged by size: 1629 1503 1425 953 889 275 154 54 52 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4338 4492 1537 Size: 275 2526 154 4090 Fragments arranged by size: 4090 2526 275 154 EarI CTCTTCn'nnn_ Cuts at: 1353 2010 3425 4975 1353 Size: 657 1415 1550 3423 Fragments arranged by size: 3423 1550 1415 657 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4114 5632 6006 6152 387 Size: 1064 138 2525 1518 374 146 1280 Fragments arranged by size: 2525 1518 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3556 3556 Size: 7045 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2219 2405 3137 3382 3601 4101 4202 Size: 393 587 186 732 245 219 500 101 Cuts at: 4202 4272 4325 5108 5705 6492 6760 1239 Size: 70 53 783 597 787 268 1524 Fragments arranged by size: 1524 787 783 732 597 587 500 393 268 245 219 186 101 70 53 EcoNI CCTnn'n_nnAGG Cuts at: 3287 3335 3287 Size: 48 6997 EcoO109I rG'GnC_Cy Cuts at: 2757 2985 3328 3478 3517 2757 Size: 228 343 150 39 6285 Fragments arranged by size: 6285 343 228 150 39 EcoRI G'AATT_C Cuts at: 2934 4354 4732 2934 Size: 1420 378 5247 Fragments arranged by size: 5247 1420 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3451 3973 3451 Size: 522 6523 FspI TGC'GCA Cuts at: 5551 5551 Size: 7045 HaeII r_GCGC'y Cuts at: 1678 4047 6192 1678 Size: 2369 2145 2531 Fragments arranged by size: 2531 2369 2145 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2112 2144 2181 2213 2323 Size: 32 135 32 540 32 37 32 110 Cuts at: 2323 2355 5686 5718 5760 5792 1373 Size: 32 3331 32 42 32 2626 Fragments arranged by size: 3331 2626 540 135 110 42 37 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4389 4635 669 Size: 988 281 2451 246 3079 Fragments arranged by size: 3079 2451 988 281 246 HindIII A'AGCT_T Cuts at: 748 3161 748 Size: 2413 4632 HpaI GTT'AAC Cuts at: 4635 4635 Size: 7045 KasI G'GCGC_C Cuts at: 4043 4043 Size: 7045 KpnI G_GTAC'C Cuts at: 4370 4370 Size: 7045 MfeI C'AATT_G Cuts at: 2496 2802 4644 2496 Size: 306 1842 4897 Fragments arranged by size: 4897 1842 306 MluI A'CGCG_T Cuts at: 6921 6921 Size: 7045 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3618 4189 6159 6343 1305 Size: 2313 571 1970 184 2007 Fragments arranged by size: 2313 2007 1970 571 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3335 3387 10 Size: 54 1425 343 1503 52 3668 Fragments arranged by size: 3668 1503 1425 343 54 52 MslI CAynn'nnrTG Cuts at: 518 1166 2522 2786 4037 5003 5362 5521 518 Size: 648 1356 264 1251 966 359 159 2042 Fragments arranged by size: 2042 1356 1251 966 648 359 264 159 MspA1I CmG'CkG Cuts at: 1606 2239 3686 3767 4461 5138 6286 6531 1606 Size: 633 1447 81 694 677 1148 245 2120 Fragments arranged by size: 2120 1447 1148 694 677 633 245 81 NarI GG'CG_CC Cuts at: 4044 4044 Size: 7045 NcoI C'CATG_G Cuts at: 513 1065 3087 3750 513 Size: 552 2022 663 3808 Fragments arranged by size: 3808 2022 663 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7045 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7045 NotI GC'GGCC_GC Cuts at: 4492 4492 Size: 7045 NspI r_CATG'y Cuts at: 1132 1229 2900 3991 4403 5948 1132 Size: 97 1671 1091 412 1545 2229 Fragments arranged by size: 2229 1671 1545 1091 412 97 PasI CC'CwG_GG Cuts at: 1348 2038 1348 Size: 690 6355 PciI A'CATG_T Cuts at: 5944 5944 Size: 7045 PflMI CCAn_nnn'nTGG Cuts at: 1108 4171 1108 Size: 3063 3982 PmeI GTTT'AAAC Cuts at: 4349 4349 Size: 7045 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5114 5146 6627 6659 5114 Size: 32 1481 32 5500 Fragments arranged by size: 5500 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2757 2985 2757 Size: 228 6817 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7045 PsiI TTA'TAA Cuts at: 4615 4615 Size: 7045 PstI C_TGCA'G Cuts at: 830 3244 3822 4397 830 Size: 2414 578 575 3478 Fragments arranged by size: 3478 2414 578 575 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7045 PvuII CAG'CTG Cuts at: 2239 3686 3767 2239 Size: 1447 81 5517 Fragments arranged by size: 5517 1447 81 RsrII CG'GwC_CG Cuts at: 4326 4326 Size: 7045 SacI G_AGCT'C Cuts at: 3558 3558 Size: 7045 SalI G'TCGA_C Cuts at: 1936 4387 1936 Size: 2451 4594 SanDI GG'GwC_CC Cuts at: 2985 2985 Size: 7045 SapI GCTCTTCn'nnn_ Cuts at: 2010 3425 2010 Size: 1415 5630 SbfI CC_TGCA'GG Cuts at: 3822 4397 3822 Size: 575 6470 ScaI AGT'ACT Cuts at: 5293 7005 5293 Size: 1712 5333 SexAI A'CCwGG_T Cuts at: 2316 2316 Size: 7045 SfcI C'TryA_G Cuts at: 826 1045 3240 3585 3818 4393 5528 6209 6400 Size: 219 2195 345 233 575 1135 681 191 Cuts at: 6400 826 Size: 1471 Fragments arranged by size: 2195 1471 1135 681 575 345 233 219 191 SfoI GGC'GCC Cuts at: 4045 4045 Size: 7045 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7045 SgrAI Cr'CCGG_yG Cuts at: 1700 2135 1700 Size: 435 6610 SmaI CCC'GGG Cuts at: 1872 2879 4372 1872 Size: 1007 1493 4545 Fragments arranged by size: 4545 1493 1007 SmlI C'TyrA_G Cuts at: 820 1017 1943 5149 6050 6312 820 Size: 197 926 3206 901 262 1553 Fragments arranged by size: 3206 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4836 493 Size: 4343 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7045 SphI G_CATG'C Cuts at: 1132 4403 1132 Size: 3271 3774 SspI AAT'ATT Cuts at: 5 52 4969 5 Size: 47 4917 2081 Fragments arranged by size: 4917 2081 47 StuI AGG'CCT Cuts at: 1517 2175 1517 Size: 658 6387 StyI C'CwwG_G Cuts at: 513 1065 2086 3081 3087 3336 3388 3750 4047 Size: 552 1021 995 6 249 52 362 297 Cuts at: 4047 4215 4484 513 Size: 168 269 3074 Fragments arranged by size: 3074 1021 995 552 362 297 269 249 168 52 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2942 3713 5072 5089 5242 5427 5872 Size: 1255 1362 771 1359 17 153 185 445 Cuts at: 5872 325 Size: 1498 Fragments arranged by size: 1498 1362 1359 1255 771 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3156 3933 5291 Size: 274 80 33 51 1443 1179 777 1358 Cuts at: 5291 7003 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1358 1179 777 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2235 2267 4211 4243 7005 7037 2235 Size: 32 1944 32 2762 32 2243 Fragments arranged by size: 2762 2243 1944 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7045 XbaI T'CTAG_A Cuts at: 4381 4381 Size: 7045 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2715 3757 2715 Size: 1042 6003 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7045 XmaI C'CCGG_G Cuts at: 1870 2877 4370 1870 Size: 1007 1493 4545 Fragments arranged by size: 4545 1493 1007 XmnI GAAnn'nnTTC Cuts at: 5174 5174 Size: 7045 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4853 276 Size: 53 83 186 936 3319 2468 Fragments arranged by size: 3319 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI SacII SfiI SrfI SwaI