Restriction map for
pFN21AB3200
(Circular) (Six-base) MAPSORT of: pFN21AB3200.seq Check: 3992 from: 1 to: 6742
pFN21AB3200 6742 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4552 278
Size: 53 83 186 936 3016 2468
Fragments arranged by size:
3016 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4085 1937
Size: 2148 4594
Acc65I G'GTAC_C
Cuts at: 4063 4063
Size: 6742
AclI AA'CG_TT
Cuts at: 1563 4869 5242 1563
Size: 3306 373 3063
Fragments arranged by size:
3306 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2253 2418 2903 3732 3780 3908 3959 4805
Size: 621 165 485 829 48 128 51 846
Cuts at: 4805 6189 6457 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 846 829 621 485 268 165
128 51 48
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6545
AflIII A'CryG_T
Cuts at: 1203 3595 5641 6618 1203
Size: 2392 2046 977 1327
Fragments arranged by size:
2392 2046 1327 977
AgeI A'CCGG_T
Cuts at: 5573 5573
Size: 6742
AhdI GACnn_n'nnGTC
Cuts at: 2330 5471 2330
Size: 3141 3601
AleI CACnn'nnGTG
Cuts at: 1166 3305 1166
Size: 2139 4603
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6742
AlwNI CAG_nnn'CTG
Cuts at: 2681 4485 6057 2681
Size: 1804 1572 3366
Fragments arranged by size:
3366 1804 1572
ApaI G_GGCC'C
Cuts at: 2066 2066
Size: 6742
ApaLI G'TGCA_C
Cuts at: 4799 5955 4799
Size: 1156 5586
ApoI r'AATT_y
Cuts at: 1493 2772 3525 3797 4051 4277 4429 6538 1493
Size: 1279 753 272 254 226 152 2109 1697
Fragments arranged by size:
2109 1697 1279 753 272 254 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2664 3378 5296 160
Size: 872 1632 714 1918 1606
Fragments arranged by size:
1918 1632 1606 872 714
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6742
AvaI C'yCGr_G
Cuts at: 1870 1943 2058 4067 1870
Size: 73 115 2009 4545
Fragments arranged by size:
4545 2009 115 73
BamHI G'GATC_C
Cuts at: 4072 4439 4072
Size: 367 6375
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2045 2252 4063 5518 618
Size: 325 217 627 258 207 1811 1455 1842
Fragments arranged by size:
1842 1811 1455 627 325 258 217 207
BanII G_rGCy'C
Cuts at: 1391 2066 2673 3223 3867 4061 1391
Size: 675 607 550 644 194 4072
Fragments arranged by size:
4072 675 644 607 550 194
BbeI G_GCGC'C
Cuts at: 2049 2049
Size: 6742
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2718 2838 3642 3723 928
Size: 971 819 120 804 81 3947
Fragments arranged by size:
3947 971 819 804 120 81
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4933 4967 1903
Size: 34 2996 34 3678
Fragments arranged by size:
3678 2996 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2806 4634 5844 2806
Size: 1828 1210 3704
Fragments arranged by size:
3704 1828 1210
BclI T'GATC_A
Cuts at: 1548 2574 1548
Size: 1026 5716
BfrBI ATG'CAT
Cuts at: 2144 2144
Size: 6742
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5353 136
Size: 107 122 71 631 605 3681 1525
Fragments arranged by size:
3681 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6737 6737
Size: 6742
BlpI GC'TnA_GC
Cuts at: 4159 4159
Size: 6742
Bme1580I G_kGCm'C
Cuts at: 1790 2066 2556 4803 5959 1790
Size: 276 490 2247 1156 2573
Fragments arranged by size:
2573 2247 1156 490 276
BmgBI CAC'GTC
Cuts at: 1318 3626 1318
Size: 2308 4434
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4014 4473 5431
Size: 416 53 83 399 24 2597 459 958
Cuts at: 5431 442
Size: 1753
Fragments arranged by size:
2597 1753 958 459 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6742
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2258 3507 5402 1239
Size: 1019 1249 1895 2579
Fragments arranged by size:
2579 1895 1249 1019
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4867 5732 6030 4867
Size: 865 298 5579
Fragments arranged by size:
5579 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5405 882
Size: 617 3906 2219
Fragments arranged by size:
3906 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4533 493
Size: 711 3329 2702
Fragments arranged by size:
3329 2702 711
BsaBI GATnn'nnATC
Cuts at: 3641 6736 3641
Size: 3095 3647
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2046 4549 4931 5566
Size: 53 83 186 936 513 2503 382 635
Cuts at: 5566 275
Size: 1451
Fragments arranged by size:
2503 1451 936 635 513 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5175 5573 5847 5994 6405 5175
Size: 398 274 147 411 5512
Fragments arranged by size:
5512 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2154 2184 2496 2526 3079
Size: 30 165 30 751 30 312 30 553
Cuts at: 3079 3109 1178
Size: 30 4811
Fragments arranged by size:
4811 751 553 312 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2953 1186
Size: 1767 4975
BseYI C'CCAG_C
Cuts at: 2584 5945 6441 2584
Size: 3361 496 2885
Fragments arranged by size:
3361 2885 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2993 1163
Size: 573 1257 4912
Fragments arranged by size:
4912 1257 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2073 4192 4953 5981 1540
Size: 275 186 72 2119 761 1028 2301
Fragments arranged by size:
2301 2119 1028 761 275 186 72
BsiHKAI G_wGCw'C
Cuts at: 3052 3867 4803 4888 5959 3052
Size: 815 936 85 1071 3835
Fragments arranged by size:
3835 1071 936 815 85
BsmI GAATG_Cn'
Cuts at: 2248 2316 4253 4346 2248
Size: 68 1937 93 4644
Fragments arranged by size:
4644 1937 93 68
BsmBI CGTCTCn'nnnn_
Cuts at: 2042 2375 2042
Size: 333 6409
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2556 2673 3052 3223 3867 4061
Size: 399 276 490 117 379 171 644 194
Cuts at: 4061 4803 4888 5959 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 644 490 399 379 276
194 171 117 85
BspEI T'CCGG_A
Cuts at: 6405 6405
Size: 6742
BspHI T'CATG_A
Cuts at: 2107 4631 6361 2107
Size: 2524 1730 2488
Fragments arranged by size:
2524 2488 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3823 4097 844
Size: 2979 274 3489
Fragments arranged by size:
3489 2979 274
BsrBI CCG'CTC
Cuts at: 1394 1823 4629 1394
Size: 429 2806 3507
Fragments arranged by size:
3507 2806 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2721 5237 5411 65
Size: 1183 1473 2516 174 1396
Fragments arranged by size:
2516 1473 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2314 2379 3290 3720 5386 5573 1700
Size: 110 504 65 911 430 1666 187 2869
Fragments arranged by size:
2869 1666 911 504 430 187 110 65
BsrGI T'GTAC_A
Cuts at: 96 3199 96
Size: 3103 3639
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6742
BssSI C'ACGA_G
Cuts at: 2039 3540 4802 5814 2039
Size: 1501 1262 1012 2967
Fragments arranged by size:
2967 1501 1262 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2567 794
Size: 1773 4969
BstBI TT'CG_AA
Cuts at: 3948 5546 3948
Size: 1598 5144
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6742
BstXI CCAn_nnnn'nTGG
Cuts at: 2639 3653 2639
Size: 1014 5728
BstYI r'GATC_y
Cuts at: 1972 3205 4072 4439 4824 4841 6293 6379 6391
Size: 1233 867 367 385 17 1452 86 12
Cuts at: 6391 6737 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1233 867 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6190
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3309 487
Size: 683 56 2083 3920
Fragments arranged by size:
3920 2083 683 56
BtsI GCAGTG_nn'
Cuts at: 4258 5052 5072 4258
Size: 794 20 5928
Fragments arranged by size:
5928 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6742
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6707
DraI TTT'AAA
Cuts at: 2431 4046 2431
Size: 1615 5127
DraIII CAC_nnn'GTG
Cuts at: 2863 2863
Size: 6742
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5749 809
Size: 507 4433 1802
Fragments arranged by size:
4433 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2070 2316 2377
Size: 54 1425 50 275 18 240 246 61
Cuts at: 2377 2629 3255 3288 4189 5078 6707 8
Size: 252 626 33 901 889 1629 43
Fragments arranged by size:
1629 1425 901 889 626 275 252 246
240 61 54 50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2070 4189 1537
Size: 275 258 2119 4090
Fragments arranged by size:
4090 2119 275 258
EarI CTCTTCn'nnn_
Cuts at: 1353 3401 3837 3927 4672 1353
Size: 2048 436 90 745 3423
Fragments arranged by size:
3423 2048 745 436 90
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5329 5703 5849 387
Size: 1064 138 3740 374 146 1280
Fragments arranged by size:
3740 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3865 3865
Size: 6742
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2253 2258 2418 2903 3507 3732 3780
Size: 393 621 5 160 485 604 225 48
Cuts at: 3780 3908 3959 4805 5402 6189 6457 1239
Size: 128 51 846 597 787 268 1524
Fragments arranged by size:
1524 846 787 621 604 597 485 393
268 225 160 128 51 48 5
EcoO109I rG'GnC_Cy
Cuts at: 2062 2063 2062
Size: 1 6741
EcoRI G'AATT_C
Cuts at: 3525 3797 4051 4429 3525
Size: 272 254 378 5838
Fragments arranged by size:
5838 378 272 254
EcoRV GAT'ATC
Cuts at: 2637 2637
Size: 6742
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3173 3173
Size: 6742
FspI TGC'GCA
Cuts at: 5248 5248
Size: 6742
HaeII r_GCGC'y
Cuts at: 1678 2049 5889 1678
Size: 371 3840 2531
Fragments arranged by size:
3840 2531 371
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2129 2161 2316 2348 2783
Size: 32 135 32 557 32 155 32 435
Cuts at: 2783 2815 3034 3066 3079 3111 5383 5415 5457
Size: 32 219 32 13 32 2272 32 42
Cuts at: 5457 5489 1373
Size: 32 2626
Fragments arranged by size:
2626 2272 557 435 219 155 135 42
32 32 32 32 32 32 32 32
32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3042 4086 4332 669
Size: 988 281 1104 1044 246 3079
Fragments arranged by size:
3079 1104 1044 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6742
HpaI GTT'AAC
Cuts at: 4332 4332
Size: 6742
KasI G'GCGC_C
Cuts at: 2045 2045
Size: 6742
KpnI G_GTAC'C
Cuts at: 4067 4067
Size: 6742
MfeI C'AATT_G
Cuts at: 4341 4341
Size: 6742
MluI A'CGCG_T
Cuts at: 6618 6618
Size: 6742
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2287 3422 5856 6040 1305
Size: 982 1135 2434 184 2007
Fragments arranged by size:
2434 2007 1135 982 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2631 3257 10
Size: 54 1425 343 799 626 3495
Fragments arranged by size:
3495 1425 799 626 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3305 4700 5059 5218 518
Size: 648 2139 1395 359 159 2042
Fragments arranged by size:
2139 2042 1395 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2678 3431 4158 4835 5983 6228 1606
Size: 1072 753 727 677 1148 245 2120
Fragments arranged by size:
2120 1148 1072 753 727 677 245
NaeI GCC'GGC
Cuts at: 2316 2381 3292 2316
Size: 65 911 5766
Fragments arranged by size:
5766 911 65
NarI GG'CG_CC
Cuts at: 2046 2046
Size: 6742
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6190
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6742
NgoMIV G'CCGG_C
Cuts at: 2314 2379 3290 2314
Size: 65 911 5766
Fragments arranged by size:
5766 911 65
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6742
NotI GC'GGCC_GC
Cuts at: 4189 4189
Size: 6742
NsiI A_TGCA'T
Cuts at: 2146 2146
Size: 6742
NspI r_CATG'y
Cuts at: 1132 1229 3599 4100 5645 1132
Size: 97 2370 501 1545 2229
Fragments arranged by size:
2370 2229 1545 501 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6742
PciI A'CATG_T
Cuts at: 3595 5641 3595
Size: 2046 4696
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6742
PfoI T'CCnGG_A
Cuts at: 2766 2766
Size: 6742
PmeI GTTT'AAAC
Cuts at: 4046 4046
Size: 6742
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4811 4843 6324 6356 4811
Size: 32 1481 32 5197
Fragments arranged by size:
5197 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6742
PsiI TTA'TAA
Cuts at: 4312 4312
Size: 6742
PspOMI G'GGCC_C
Cuts at: 2062 2062
Size: 6742
PstI C_TGCA'G
Cuts at: 830 2029 2539 4094 830
Size: 1199 510 1555 3478
Fragments arranged by size:
3478 1555 1199 510
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6742
PvuII CAG'CTG
Cuts at: 2678 2678
Size: 6742
SacI G_AGCT'C
Cuts at: 3867 3867
Size: 6742
SalI G'TCGA_C
Cuts at: 1936 4084 1936
Size: 2148 4594
SapI GCTCTTCn'nnn_
Cuts at: 3401 3401
Size: 6742
SbfI CC_TGCA'GG
Cuts at: 4094 4094
Size: 6742
ScaI AGT'ACT
Cuts at: 4990 6702 4990
Size: 1712 5030
SfcI C'TryA_G
Cuts at: 826 1045 2025 2535 4090 5225 5906 6097 826
Size: 219 980 510 1555 1135 681 191 1471
Fragments arranged by size:
1555 1471 1135 980 681 510 219 191
SfoI GGC'GCC
Cuts at: 2047 2047
Size: 6742
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6742
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6742
SmaI CCC'GGG
Cuts at: 1872 2060 4069 1872
Size: 188 2009 4545
Fragments arranged by size:
4545 2009 188
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4846 5747 6009 820
Size: 197 926 2903 901 262 1553
Fragments arranged by size:
2903 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4533 493
Size: 4040 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6742
SphI G_CATG'C
Cuts at: 1132 4100 1132
Size: 2968 3774
SspI AAT'ATT
Cuts at: 5 52 3901 4666 5
Size: 47 3849 765 2081
Fragments arranged by size:
3849 2081 765 47
StuI AGG'CCT
Cuts at: 1517 2846 1517
Size: 1329 5413
StyI C'CwwG_G
Cuts at: 513 1065 3183 3502 4181 513
Size: 552 2118 319 679 3074
Fragments arranged by size:
3074 2118 679 552 319
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3152 4769 4786 4939 5124 5569 325
Size: 1255 1572 1617 17 153 185 445 1498
Fragments arranged by size:
1617 1572 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2401 3199 3592
Size: 274 80 33 51 1443 424 798 393
Cuts at: 3592 4988 6700 96
Size: 1396 1712 138
Fragments arranged by size:
1712 1443 1396 798 424 393 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2496 2528 2634 2666 6702 6734 2496
Size: 32 106 32 4036 32 2504
Fragments arranged by size:
4036 2504 106 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2717 1316
Size: 1401 5341
XbaI T'CTAG_A
Cuts at: 2698 4078 2698
Size: 1380 5362
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2200 3653 2200
Size: 1453 5289
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6742
XmaI C'CCGG_G
Cuts at: 1870 2058 4067 1870
Size: 188 2009 4545
Fragments arranged by size:
4545 2009 188
XmnI GAAnn'nnTTC
Cuts at: 2870 3153 3944 4871 2870
Size: 283 791 927 4741
Fragments arranged by size:
4741 927 791 283
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4550 276
Size: 53 83 186 936 3016 2468
Fragments arranged by size:
3016 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BplI
Bpu10I BsiWI BstZ17I Bsu36I EcoNI FseI FspAI NruI
PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SrfI SwaI