Restriction map for
pFN21AB3111
(Circular) (Six-base) MAPSORT of: pFN21AB3111.seq Check: 4370 from: 1 to: 5788
pFN21AB3111 5788 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2564 2564
Size: 5788
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3598 278
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2287 3131 1937
Size: 350 844 4594
Fragments arranged by size:
4594 844 350
Acc65I G'GTAC_C
Cuts at: 3109 3109
Size: 5788
AclI AA'CG_TT
Cuts at: 1563 3915 4288 1563
Size: 2352 373 3063
Fragments arranged by size:
3063 2352 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3851 5235 5503 1632
Size: 2219 1384 268 1917
Fragments arranged by size:
2219 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5591
AflIII A'CryG_T
Cuts at: 1203 4687 5664 1203
Size: 3484 977 1327
Fragments arranged by size:
3484 1327 977
AgeI A'CCGG_T
Cuts at: 2012 4619 2012
Size: 2607 3181
AhdI GACnn_n'nnGTC
Cuts at: 2294 4517 2294
Size: 2223 3565
AleI CACnn'nnGTG
Cuts at: 1166 2015 1166
Size: 849 4939
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5788
AlwNI CAG_nnn'CTG
Cuts at: 3531 5103 3531
Size: 1572 4216
ApaLI G'TGCA_C
Cuts at: 2136 3845 5001 2136
Size: 1709 1156 2923
Fragments arranged by size:
2923 1709 1156
ApoI r'AATT_y
Cuts at: 1493 3097 3323 3475 5584 1493
Size: 1604 226 152 2109 1697
Fragments arranged by size:
2109 1697 1604 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4342 160
Size: 872 3310 1606
Fragments arranged by size:
3310 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
AvaI C'yCGr_G
Cuts at: 1870 1943 3113 1870
Size: 73 1170 4545
Fragments arranged by size:
4545 1170 73
BamHI G'GATC_C
Cuts at: 3118 3485 3118
Size: 367 5421
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2912 3109 4564 618
Size: 325 217 627 1125 197 1455 1842
Fragments arranged by size:
1842 1455 1125 627 325 217 197
BanII G_rGCy'C
Cuts at: 1391 2392 3107 1391
Size: 1001 715 4072
Fragments arranged by size:
4072 1001 715
BbeI G_GCGC'C
Cuts at: 2916 2916
Size: 5788
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4817
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2071 2105 3979 4013 1903
Size: 34 134 34 1874 34 3678
Fragments arranged by size:
3678 1874 134 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3680 4890 3680
Size: 1210 4578
BclI T'GATC_A
Cuts at: 1548 2226 1548
Size: 678 5110
BfrBI ATG'CAT
Cuts at: 2065 2840 2065
Size: 775 5013
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4399 136
Size: 107 122 71 631 605 2727 1525
Fragments arranged by size:
2727 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2487 5783 2487
Size: 3296 2492
BlpI GC'TnA_GC
Cuts at: 3205 3205
Size: 5788
Bme1580I G_kGCm'C
Cuts at: 1790 2140 3849 5005 1790
Size: 350 1709 1156 2573
Fragments arranged by size:
2573 1709 1156 350
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5788
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2963 3519 4477
Size: 416 53 83 399 24 1546 556 958
Cuts at: 4477 442
Size: 1753
Fragments arranged by size:
1753 1546 958 556 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2678 2793 4448 1239
Size: 1439 115 1655 2579
Fragments arranged by size:
2579 1655 1439 115
Bpu10I CC'TnA_GC
Cuts at: 2391 2391
Size: 5788
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3913 4778 5076 3913
Size: 865 298 4625
Fragments arranged by size:
4625 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4451 882
Size: 617 2952 2219
Fragments arranged by size:
2952 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2141 3579 493
Size: 711 937 1438 2702
Fragments arranged by size:
2702 1438 937 711
BsaBI GATnn'nnATC
Cuts at: 5782 5782
Size: 5788
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2905 2913 3595 3977
Size: 53 83 186 936 1372 8 682 382
Cuts at: 3977 4612 275
Size: 635 1451
Fragments arranged by size:
1451 1372 936 682 635 382 186 83
53 8
BsaWI w'CCGG_w
Cuts at: 2012 2560 2780 4221 4619 4893 5040 5451 2012
Size: 548 220 1441 398 274 147 411 2349
Fragments arranged by size:
2349 1441 548 411 398 274 220 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2094 2124 1178
Size: 30 165 30 691 30 4842
Fragments arranged by size:
4842 691 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2141 1186
Size: 955 4833
BseYI C'CCAG_C
Cuts at: 4991 5487 4991
Size: 496 5292
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2714 1163
Size: 573 978 4237
Fragments arranged by size:
4237 978 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3238 3999 5027 1540
Size: 275 186 1237 761 1028 2301
Fragments arranged by size:
2301 1237 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2140 3849 3934 5005 2140
Size: 1709 85 1071 2923
Fragments arranged by size:
2923 1709 1071 85
BsmI GAATG_Cn'
Cuts at: 2454 3299 3392 2454
Size: 845 93 4850
Fragments arranged by size:
4850 845 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3059 3059
Size: 5788
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2140 2392 3107 3849 3934 5005 1391
Size: 399 350 252 715 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 715 399 350 252 85
BspEI T'CCGG_A
Cuts at: 5451 5451
Size: 5788
BspHI T'CATG_A
Cuts at: 2860 3677 5407 2860
Size: 817 1730 3241
Fragments arranged by size:
3241 1730 817
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2564 2983 3143 844
Size: 1720 419 160 3489
Fragments arranged by size:
3489 1720 419 160
BsrBI CCG'CTC
Cuts at: 1394 1823 2434 3675 1394
Size: 429 611 1241 3507
Fragments arranged by size:
3507 1241 611 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2748 4283 4457 65
Size: 1183 1500 1535 174 1396
Fragments arranged by size:
1535 1500 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2012 2538 4432 4619 1700
Size: 110 202 526 1894 187 2869
Fragments arranged by size:
2869 1894 526 202 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5788
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5788
BssSI C'ACGA_G
Cuts at: 3848 4860 3848
Size: 1012 4776
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5788
BstBI TT'CG_AA
Cuts at: 4592 4592
Size: 5788
BstEII G'GTnAC_C
Cuts at: 1184 2296 2995 1184
Size: 1112 699 3977
Fragments arranged by size:
3977 1112 699
BstYI r'GATC_y
Cuts at: 1972 2487 2928 2978 3008 3118 3485 3870 3887
Size: 515 441 50 30 110 367 385 17
Cuts at: 3887 5339 5425 5437 5783 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 515 441 385 367 346 110
86 50 30 17 12
Bsu36I CC'TnA_GG
Cuts at: 2303 2303
Size: 5788
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4297
Fragments arranged by size:
4297 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5049
Fragments arranged by size:
5049 683 56
BtsI GCAGTG_nn'
Cuts at: 3304 4098 4118 3304
Size: 794 20 4974
Fragments arranged by size:
4974 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5788
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5753
DraI TTT'AAA
Cuts at: 3092 3092
Size: 5788
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4795 809
Size: 507 3479 1802
Fragments arranged by size:
3479 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3235 4124 5753
Size: 54 1425 50 275 18 1405 889 1629
Cuts at: 5753 8
Size: 43
Fragments arranged by size:
1629 1425 1405 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3235 1537
Size: 275 1423 4090
Fragments arranged by size:
4090 1423 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2238 3718 1353
Size: 885 1480 3423
Fragments arranged by size:
3423 1480 885
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4375 4749 4895 387
Size: 1064 138 2786 374 146 1280
Fragments arranged by size:
2786 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2678 2793 3851 4448 5235 5503 1239
Size: 393 1046 115 1058 597 787 268 1524
Fragments arranged by size:
1524 1058 1046 787 597 393 268 115
EcoRI G'AATT_C
Cuts at: 3097 3475 3097
Size: 378 5410
FspI TGC'GCA
Cuts at: 4294 4294
Size: 5788
HaeII r_GCGC'y
Cuts at: 1678 2916 4935 1678
Size: 1238 2019 2531
Fragments arranged by size:
2531 2019 1238
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2022 2054 2280 2312 2346
Size: 32 135 32 450 32 226 32 34
Cuts at: 2346 2378 2842 2874 4429 4461 4503 4535 1373
Size: 32 464 32 1555 32 42 32 2626
Fragments arranged by size:
2626 1555 464 450 226 135 42 34
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3132 3378 669
Size: 988 281 1194 246 3079
Fragments arranged by size:
3079 1194 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5788
HpaI GTT'AAC
Cuts at: 3378 3378
Size: 5788
KasI G'GCGC_C
Cuts at: 2912 2912
Size: 5788
KpnI G_GTAC'C
Cuts at: 3113 3113
Size: 5788
MfeI C'AATT_G
Cuts at: 3387 3387
Size: 5788
MluI A'CGCG_T
Cuts at: 5664 5664
Size: 5788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2484 4902 5086 1305
Size: 1179 2418 184 2007
Fragments arranged by size:
2418 2007 1179 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3966
Fragments arranged by size:
3966 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2015 3746 4105 4264 518
Size: 648 849 1731 359 159 2042
Fragments arranged by size:
2042 1731 849 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2279 3204 3881 5029 5274 1606
Size: 673 925 677 1148 245 2120
Fragments arranged by size:
2120 1148 925 677 673 245
NarI GG'CG_CC
Cuts at: 2913 2913
Size: 5788
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4297
Fragments arranged by size:
4297 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5788
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5788
NotI GC'GGCC_GC
Cuts at: 3235 3235
Size: 5788
NsiI A_TGCA'T
Cuts at: 2067 2842 2067
Size: 775 5013
NspI r_CATG'y
Cuts at: 1132 1229 3146 4691 1132
Size: 97 1917 1545 2229
Fragments arranged by size:
2229 1917 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5788
PciI A'CATG_T
Cuts at: 4687 4687
Size: 5788
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5788
PmeI GTTT'AAAC
Cuts at: 3092 3092
Size: 5788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3857 3889 5370 5402 3857
Size: 32 1481 32 4243
Fragments arranged by size:
4243 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5788
PsiI TTA'TAA
Cuts at: 3358 3358
Size: 5788
PstI C_TGCA'G
Cuts at: 830 3140 830
Size: 2310 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5788
PvuII CAG'CTG
Cuts at: 2279 2279
Size: 5788
SalI G'TCGA_C
Cuts at: 1936 3130 1936
Size: 1194 4594
SbfI CC_TGCA'GG
Cuts at: 3140 3140
Size: 5788
ScaI AGT'ACT
Cuts at: 4036 5748 4036
Size: 1712 4076
SfcI C'TryA_G
Cuts at: 826 1045 3136 4271 4952 5143 826
Size: 219 2091 1135 681 191 1471
Fragments arranged by size:
2091 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2914 2914
Size: 5788
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
SgrAI Cr'CCGG_yG
Cuts at: 1700 2012 1700
Size: 312 5476
SmaI CCC'GGG
Cuts at: 1872 3115 1872
Size: 1243 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3892 4793 5055 820
Size: 197 926 1949 901 262 1553
Fragments arranged by size:
1949 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3579 493
Size: 3086 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5788
SphI G_CATG'C
Cuts at: 1132 3146 1132
Size: 2014 3774
SspI AAT'ATT
Cuts at: 5 52 3712 5
Size: 47 3660 2081
Fragments arranged by size:
3660 2081 47
StuI AGG'CCT
Cuts at: 1517 2678 1517
Size: 1161 4627
StyI C'CwwG_G
Cuts at: 513 1065 2004 3227 513
Size: 552 939 1223 3074
Fragments arranged by size:
3074 1223 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3815 3832 3985 4170 4615 325
Size: 1255 2235 17 153 185 445 1498
Fragments arranged by size:
2235 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2381 4034 5746
Size: 274 80 33 51 1443 404 1653 1712
Cuts at: 5746 96
Size: 138
Fragments arranged by size:
1712 1653 1443 404 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5748 5780 5748
Size: 32 5756
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5788
XbaI T'CTAG_A
Cuts at: 2671 3124 2671
Size: 453 5335
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3025 3025
Size: 5788
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5788
XmaI C'CCGG_G
Cuts at: 1870 3113 1870
Size: 1243 4545
XmnI GAAnn'nnTTC
Cuts at: 3917 3917
Size: 5788
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3596 276
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI
BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI