(Circular) (Six-base) MAPSORT of: pFN21AB3110.seq Check: 9785 from: 1 to: 5596 pFN21AB3110 5596 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3406 278 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2939 1937 Size: 1002 4594 Acc65I G'GTAC_C Cuts at: 2112 2624 2917 2112 Size: 512 293 4791 Fragments arranged by size: 4791 512 293 AclI AA'CG_TT Cuts at: 1563 3723 4096 1563 Size: 2160 373 3063 Fragments arranged by size: 3063 2160 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3659 5043 5311 1632 Size: 2027 1384 268 1917 Fragments arranged by size: 2027 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5399 AflIII A'CryG_T Cuts at: 1203 2744 4495 5472 1203 Size: 1541 1751 977 1327 Fragments arranged by size: 1751 1541 1327 977 AgeI A'CCGG_T Cuts at: 4427 4427 Size: 5596 AhdI GACnn_n'nnGTC Cuts at: 4325 4325 Size: 5596 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5596 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5596 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2510 2542 2510 Size: 32 5564 AlwNI CAG_nnn'CTG Cuts at: 2089 2098 3339 4911 2089 Size: 9 1241 1572 2774 Fragments arranged by size: 2774 1572 1241 9 ApaI G_GGCC'C Cuts at: 2071 2071 Size: 5596 ApaLI G'TGCA_C Cuts at: 3653 4809 3653 Size: 1156 4440 ApoI r'AATT_y Cuts at: 1493 2905 3131 3283 5392 1493 Size: 1412 226 152 2109 1697 Fragments arranged by size: 2109 1697 1412 226 152 AseI AT'TA_AT Cuts at: 160 1032 4150 160 Size: 872 3118 1606 Fragments arranged by size: 3118 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 AvaI C'yCGr_G Cuts at: 1870 1943 2167 2234 2553 2585 2592 2858 2921 Size: 73 224 67 319 32 7 266 63 Cuts at: 2921 1870 Size: 4545 Fragments arranged by size: 4545 319 266 224 73 67 63 32 7 BamHI G'GATC_C Cuts at: 2926 3293 2926 Size: 367 5229 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2112 2171 2576 2624 2917 Size: 325 217 627 325 59 405 48 293 Cuts at: 2917 4372 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 405 325 325 293 217 59 48 BanII G_rGCy'C Cuts at: 1391 2071 2586 2592 2681 2915 1391 Size: 680 515 6 89 234 4072 Fragments arranged by size: 4072 680 515 234 89 6 BbeI G_GCGC'C Cuts at: 2175 2175 Size: 5596 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2894 928 Size: 971 995 3630 Fragments arranged by size: 3630 995 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3787 3821 1903 Size: 34 1850 34 3678 Fragments arranged by size: 3678 1850 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3488 4698 3488 Size: 1210 4386 BclI T'GATC_A Cuts at: 1548 1548 Size: 5596 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2271 4207 136 Size: 107 122 71 631 605 599 1936 1525 Fragments arranged by size: 1936 1525 631 605 599 122 107 71 BglII A'GATC_T Cuts at: 5591 5591 Size: 5596 BlpI GC'TnA_GC Cuts at: 2429 2472 3013 2429 Size: 43 541 5012 Fragments arranged by size: 5012 541 43 Bme1580I G_kGCm'C Cuts at: 1790 2071 2814 3657 4813 1790 Size: 281 743 843 1156 2573 Fragments arranged by size: 2573 1156 843 743 281 BmgBI CAC'GTC Cuts at: 1318 2293 2809 1318 Size: 975 516 4105 Fragments arranged by size: 4105 975 516 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3327 4285 442 Size: 416 53 83 399 24 1910 958 1753 Fragments arranged by size: 1910 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5596 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2690 4256 1239 Size: 1451 1566 2579 Fragments arranged by size: 2579 1566 1451 Bpu10I CC'TnA_GC Cuts at: 2654 2654 Size: 5596 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3721 4586 4884 3721 Size: 865 298 4433 Fragments arranged by size: 4433 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4259 882 Size: 617 2760 2219 Fragments arranged by size: 2760 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2745 2773 3387 493 Size: 711 1541 28 614 2702 Fragments arranged by size: 2702 1541 711 614 28 BsaBI GATnn'nnATC Cuts at: 5590 5590 Size: 5596 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2103 2172 2307 2757 Size: 53 83 186 936 570 69 135 450 Cuts at: 2757 3403 3785 4420 275 Size: 646 382 635 1451 Fragments arranged by size: 1451 936 646 635 570 450 382 186 135 83 69 53 BsaWI w'CCGG_w Cuts at: 4029 4427 4701 4848 5259 4029 Size: 398 274 147 411 4366 Fragments arranged by size: 4366 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2810 2840 1178 Size: 30 165 30 1407 30 3934 Fragments arranged by size: 3934 1407 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2168 2699 1186 Size: 982 531 4083 Fragments arranged by size: 4083 982 531 BseYI C'CCAG_C Cuts at: 2078 2125 4799 5295 2078 Size: 47 2674 496 2379 Fragments arranged by size: 2674 2379 496 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2218 1163 Size: 573 482 4541 Fragments arranged by size: 4541 573 482 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2206 3046 3807 4835 1540 Size: 275 186 205 840 761 1028 2301 Fragments arranged by size: 2301 1028 840 761 275 205 186 BsiHKAI G_wGCw'C Cuts at: 3657 3742 4813 3657 Size: 85 1071 4440 Fragments arranged by size: 4440 1071 85 BsmI GAATG_Cn' Cuts at: 3107 3200 3107 Size: 93 5503 BsmBI CGTCTCn'nnnn_ Cuts at: 2589 2589 Size: 5596 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2071 2586 2592 2681 2814 2915 3657 Size: 399 281 515 6 89 133 101 742 Cuts at: 3657 3742 4813 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 515 399 281 133 101 89 85 6 BspEI T'CCGG_A Cuts at: 5259 5259 Size: 5596 BspHI T'CATG_A Cuts at: 3485 5215 3485 Size: 1730 3866 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2124 2458 2556 2951 844 Size: 1280 334 98 395 3489 Fragments arranged by size: 3489 1280 395 334 98 BsrBI CCG'CTC Cuts at: 1394 1823 2428 3483 1394 Size: 429 605 1055 3507 Fragments arranged by size: 3507 1055 605 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2051 4091 4265 65 Size: 1183 803 2040 174 1396 Fragments arranged by size: 2040 1396 1183 803 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4240 4427 1700 Size: 110 2430 187 2869 Fragments arranged by size: 2869 2430 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5596 BssHII G'CGCG_C Cuts at: 1925 2028 2074 2848 1925 Size: 103 46 774 4673 Fragments arranged by size: 4673 774 103 46 BssSI C'ACGA_G Cuts at: 3656 4668 3656 Size: 1012 4584 BstAPI GCAn_nnn'nTGC Cuts at: 794 2089 794 Size: 1295 4301 BstBI TT'CG_AA Cuts at: 4400 4400 Size: 5596 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5596 BstYI r'GATC_y Cuts at: 1972 2688 2926 3293 3678 3695 5147 5233 5245 Size: 716 238 367 385 17 1452 86 12 Cuts at: 5245 5591 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 716 385 367 346 238 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2682 2682 Size: 5596 BtgI C'CryG_G Cuts at: 513 1065 2004 2572 513 Size: 552 939 568 3537 Fragments arranged by size: 3537 939 568 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4857 Fragments arranged by size: 4857 683 56 BtsI GCAGTG_nn' Cuts at: 2381 3112 3906 3926 2381 Size: 731 794 20 4051 Fragments arranged by size: 4051 794 731 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5596 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5561 DraI TTT'AAA Cuts at: 2900 2900 Size: 5596 DraIII CAC_nnn'GTG Cuts at: 2296 2377 2296 Size: 81 5515 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4603 809 Size: 507 3287 1802 Fragments arranged by size: 3287 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2283 2365 2611 Size: 54 1425 50 275 18 453 82 246 Cuts at: 2611 2739 3043 3932 5561 8 Size: 128 304 889 1629 43 Fragments arranged by size: 1629 1425 889 453 304 275 246 128 82 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3043 1537 Size: 275 1231 4090 Fragments arranged by size: 4090 1231 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2669 3526 1353 Size: 1316 857 3423 Fragments arranged by size: 3423 1316 857 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2069 2320 2390 2681 4183 4557 Size: 1064 138 480 251 70 291 1502 374 Cuts at: 4557 4703 387 Size: 146 1280 Fragments arranged by size: 1502 1280 1064 480 374 291 251 146 138 70 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2690 3659 4256 5043 5311 1239 Size: 393 1058 969 597 787 268 1524 Fragments arranged by size: 1524 1058 969 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2657 2657 Size: 5596 EcoO109I rG'GnC_Cy Cuts at: 2068 2320 2784 2068 Size: 252 464 4880 Fragments arranged by size: 4880 464 252 EcoRI G'AATT_C Cuts at: 2905 3283 2905 Size: 378 5218 FspI TGC'GCA Cuts at: 4102 4102 Size: 5596 HaeII r_GCGC'y Cuts at: 1678 2063 2161 2175 2536 4743 1678 Size: 385 98 14 361 2207 2531 Fragments arranged by size: 2531 2207 385 361 98 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2195 2227 2680 2712 2855 Size: 32 135 32 623 32 453 32 143 Cuts at: 2855 2887 4237 4269 4311 4343 1373 Size: 32 1350 32 42 32 2626 Fragments arranged by size: 2626 1350 623 453 143 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2940 3186 669 Size: 988 281 1002 246 3079 Fragments arranged by size: 3079 1002 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5596 HpaI GTT'AAC Cuts at: 3186 3186 Size: 5596 KasI G'GCGC_C Cuts at: 2171 2171 Size: 5596 KpnI G_GTAC'C Cuts at: 2116 2628 2921 2116 Size: 512 293 4791 Fragments arranged by size: 4791 512 293 MfeI C'AATT_G Cuts at: 3195 3195 Size: 5596 MluI A'CGCG_T Cuts at: 5472 5472 Size: 5596 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4710 4894 1305 Size: 3405 184 2007 Fragments arranged by size: 3405 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2613 2741 10 Size: 54 1425 343 781 128 2865 Fragments arranged by size: 2865 1425 781 343 128 54 MslI CAynn'nnrTG Cuts at: 518 1166 3554 3913 4072 518 Size: 648 2388 359 159 2042 Fragments arranged by size: 2388 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2095 2574 3012 3689 4837 5082 1606 Size: 489 479 438 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 489 479 438 245 NarI GG'CG_CC Cuts at: 2172 2172 Size: 5596 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4105 Fragments arranged by size: 4105 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5596 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5596 NotI GC'GGCC_GC Cuts at: 3043 3043 Size: 5596 NspI r_CATG'y Cuts at: 1132 1229 2230 2848 2954 4499 1132 Size: 97 1001 618 106 1545 2229 Fragments arranged by size: 2229 1545 1001 618 106 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5596 PciI A'CATG_T Cuts at: 4495 4495 Size: 5596 PflMI CCAn_nnn'nTGG Cuts at: 1108 2545 1108 Size: 1437 4159 PfoI T'CCnGG_A Cuts at: 2703 2703 Size: 5596 PmeI GTTT'AAAC Cuts at: 2900 2900 Size: 5596 PmlI CAC'GTG Cuts at: 2745 2773 2745 Size: 28 5568 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3665 3697 5178 5210 3665 Size: 32 1481 32 4051 Fragments arranged by size: 4051 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5596 PsiI TTA'TAA Cuts at: 3166 3166 Size: 5596 PspOMI G'GGCC_C Cuts at: 2067 2067 Size: 5596 PstI C_TGCA'G Cuts at: 830 2094 2948 830 Size: 1264 854 3478 Fragments arranged by size: 3478 1264 854 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5596 PvuII CAG'CTG Cuts at: 2095 2095 Size: 5596 SacII CC_GC'GG Cuts at: 2575 2575 Size: 5596 SalI G'TCGA_C Cuts at: 1936 2938 1936 Size: 1002 4594 SapI GCTCTTCn'nnn_ Cuts at: 2669 2669 Size: 5596 SbfI CC_TGCA'GG Cuts at: 2948 2948 Size: 5596 ScaI AGT'ACT Cuts at: 3844 5556 3844 Size: 1712 3884 SfcI C'TryA_G Cuts at: 826 1045 2090 2944 4079 4760 4951 826 Size: 219 1045 854 1135 681 191 1471 Fragments arranged by size: 1471 1135 1045 854 681 219 191 SfoI GGC'GCC Cuts at: 2173 2173 Size: 5596 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5596 SmaI CCC'GGG Cuts at: 1872 2169 2236 2923 1872 Size: 297 67 687 4545 Fragments arranged by size: 4545 687 297 67 SmlI C'TyrA_G Cuts at: 820 1017 1943 3700 4601 4863 820 Size: 197 926 1757 901 262 1553 Fragments arranged by size: 1757 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3387 493 Size: 2894 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5596 SphI G_CATG'C Cuts at: 1132 2954 1132 Size: 1822 3774 SspI AAT'ATT Cuts at: 5 52 3520 5 Size: 47 3468 2081 Fragments arranged by size: 3468 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5596 StyI C'CwwG_G Cuts at: 513 1065 2004 3035 513 Size: 552 939 1031 3074 Fragments arranged by size: 3074 1031 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2038 2303 2821 3623 3640 3793 3978 Size: 1255 458 265 518 802 17 153 185 Cuts at: 3978 4423 325 Size: 445 1498 Fragments arranged by size: 1498 1255 802 518 458 445 265 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3842 5554 96 Size: 274 80 33 51 1443 1865 1712 138 Fragments arranged by size: 1865 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5556 5588 5556 Size: 32 5564 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5596 XbaI T'CTAG_A Cuts at: 2932 2932 Size: 5596 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5596 XmaI C'CCGG_G Cuts at: 1870 2167 2234 2921 1870 Size: 297 67 687 4545 Fragments arranged by size: 4545 687 297 67 XmnI GAAnn'nnTTC Cuts at: 3725 3725 Size: 5596 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3404 276 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SrfI SwaI XcmI