Restriction map for
pFN21AB3064
(Circular) (Six-base) MAPSORT of: pFN21AB3064.seq Check: 5193 from: 1 to: 5935
pFN21AB3064 5935 bp
With 182 enzymes: SgfI *
October 20, 2009 10:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3745 278
Size: 53 83 186 936 2209 2468
Fragments arranged by size:
2468 2209 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3278 1937
Size: 1341 4594
Acc65I G'GTAC_C
Cuts at: 3256 3256
Size: 5935
AclI AA'CG_TT
Cuts at: 1563 4062 4435 1563
Size: 2499 373 3063
Fragments arranged by size:
3063 2499 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2367 2577 2907 3998 5382 5650 1632
Size: 735 210 330 1091 1384 268 1917
Fragments arranged by size:
1917 1384 1091 735 330 268 210
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5738
AflIII A'CryG_T
Cuts at: 1203 3091 4834 5811 1203
Size: 1888 1743 977 1327
Fragments arranged by size:
1888 1743 1327 977
AgeI A'CCGG_T
Cuts at: 4766 4766
Size: 5935
AhdI GACnn_n'nnGTC
Cuts at: 4664 4664
Size: 5935
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5935
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5935
AlwNI CAG_nnn'CTG
Cuts at: 2459 2900 3678 5250 2459
Size: 441 778 1572 3144
Fragments arranged by size:
3144 1572 778 441
ApaLI G'TGCA_C
Cuts at: 3992 5148 3992
Size: 1156 4779
ApoI r'AATT_y
Cuts at: 1493 2237 3244 3470 3622 5731 1493
Size: 744 1007 226 152 2109 1697
Fragments arranged by size:
2109 1697 1007 744 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4489 160
Size: 872 3457 1606
Fragments arranged by size:
3457 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
AvaI C'yCGr_G
Cuts at: 1870 1943 2340 3260 1870
Size: 73 397 920 4545
Fragments arranged by size:
4545 920 397 73
BamHI G'GATC_C
Cuts at: 2945 3265 3632 2945
Size: 320 367 5248
Fragments arranged by size:
5248 367 320
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3178 3256 4711 618
Size: 325 217 627 1391 78 1455 1842
Fragments arranged by size:
1842 1455 1391 627 325 217 78
BanII G_rGCy'C
Cuts at: 1391 2820 2860 3254 1391
Size: 1429 40 394 4072
Fragments arranged by size:
4072 1429 394 40
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2693 3131 928
Size: 971 794 438 3732
Fragments arranged by size:
3732 971 794 438
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4126 4160 1903
Size: 34 2189 34 3678
Fragments arranged by size:
3678 2189 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3827 5037 3827
Size: 1210 4725
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5935
BfrBI ATG'CAT
Cuts at: 2312 2556 2847 3120 2312
Size: 244 291 273 5127
Fragments arranged by size:
5127 291 273 244
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4546 136
Size: 107 122 71 631 605 2874 1525
Fragments arranged by size:
2874 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5930 5930
Size: 5935
BlpI GC'TnA_GC
Cuts at: 2814 3352 2814
Size: 538 5397
Bme1580I G_kGCm'C
Cuts at: 1790 3996 5152 1790
Size: 2206 1156 2573
Fragments arranged by size:
2573 2206 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5935
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3031 3666 4624
Size: 416 53 83 399 24 1614 635 958
Cuts at: 4624 442
Size: 1753
Fragments arranged by size:
1753 1614 958 635 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5935
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2442 2844 4595 1239
Size: 1203 402 1751 2579
Fragments arranged by size:
2579 1751 1203 402
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2620 4060 4925 5223 2620
Size: 1440 865 298 3332
Fragments arranged by size:
3332 1440 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2265 2380 4598 882
Size: 617 766 115 2218 2219
Fragments arranged by size:
2219 2218 766 617 115
BsaAI yAC'GTr
Cuts at: 493 1204 2717 3029 3726 493
Size: 711 1513 312 697 2702
Fragments arranged by size:
2702 1513 711 697 312
BsaBI GATnn'nnATC
Cuts at: 5929 5929
Size: 5935
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3742 4124 4759 275
Size: 53 83 186 936 2209 382 635 1451
Fragments arranged by size:
2209 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4368 4766 5040 5187 5598 4368
Size: 398 274 147 411 4705
Fragments arranged by size:
4705 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5710
Fragments arranged by size:
5710 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5935
BseYI C'CCAG_C
Cuts at: 5138 5634 5138
Size: 496 5439
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5362
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3385 4146 5174 1540
Size: 275 186 1384 761 1028 2301
Fragments arranged by size:
2301 1384 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2820 3996 4081 5152 2820
Size: 1176 85 1071 3603
Fragments arranged by size:
3603 1176 1071 85
BsmI GAATG_Cn'
Cuts at: 2558 2810 2849 3446 3539 2558
Size: 252 39 597 93 4954
Fragments arranged by size:
4954 597 252 93 39
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2820 2860 3254 3996 4081 5152 1391
Size: 399 1030 40 394 742 85 1071 2174
Fragments arranged by size:
2174 1071 1030 742 399 394 85 40
BspEI T'CCGG_A
Cuts at: 5598 5598
Size: 5935
BspHI T'CATG_A
Cuts at: 3013 3824 5554 3013
Size: 811 1730 3394
Fragments arranged by size:
3394 1730 811
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3290 844
Size: 2446 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3822 1394
Size: 429 1999 3507
Fragments arranged by size:
3507 1999 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4430 4604 65
Size: 1183 3182 174 1396
Fragments arranged by size:
3182 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4579 4766 1700
Size: 110 2769 187 2869
Fragments arranged by size:
2869 2769 187 110
BsrGI T'GTAC_A
Cuts at: 96 2461 96
Size: 2365 3570
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5935
BssSI C'ACGA_G
Cuts at: 3005 3995 5007 3005
Size: 990 1012 3933
Fragments arranged by size:
3933 1012 990
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5935
BstBI TT'CG_AA
Cuts at: 4739 4739
Size: 5935
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5935
BstXI CCAn_nnnn'nTGG
Cuts at: 2283 2283
Size: 5935
BstYI r'GATC_y
Cuts at: 1972 2897 2945 3265 3632 4017 4034 5486 5572
Size: 925 48 320 367 385 17 1452 86
Cuts at: 5572 5584 5930 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 925 385 367 346 320 86
48 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4444
Fragments arranged by size:
4444 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3130 487
Size: 683 56 1904 3292
Fragments arranged by size:
3292 1904 683 56
BtsI GCAGTG_nn'
Cuts at: 3451 4245 4265 3451
Size: 794 20 5121
Fragments arranged by size:
5121 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5935
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5900
DraI TTT'AAA
Cuts at: 2098 3239 2098
Size: 1141 4794
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2259 4942 809
Size: 507 943 2683 1802
Fragments arranged by size:
2683 1802 943 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2572 2726 3382
Size: 54 1425 50 275 18 742 154 656
Cuts at: 3382 4271 5900 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 742 656 275 154 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3382 1537
Size: 275 1570 4090
Fragments arranged by size:
4090 1570 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2073 3865 1353
Size: 720 1792 3423
Fragments arranged by size:
3423 1792 720
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2286 4522 4896 5042 387
Size: 1064 138 697 2236 374 146 1280
Fragments arranged by size:
2236 1280 1064 697 374 146 138
EcoICRI GAG'CTC
Cuts at: 2818 2818
Size: 5935
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2367 2442 2577 2844 2907 3998 4595
Size: 393 735 75 135 267 63 1091 597
Cuts at: 4595 5382 5650 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1091 787 735 597 393 268 267
135 75 63
EcoRI G'AATT_C
Cuts at: 3244 3622 3244
Size: 378 5557
FspI TGC'GCA
Cuts at: 3138 4441 3138
Size: 1303 4632
FspAI rTGC'GCAy
Cuts at: 3138 3138
Size: 5935
HaeII r_GCGC'y
Cuts at: 1678 5082 1678
Size: 3404 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2278 2310 4576 4608 4650
Size: 32 135 32 706 32 2266 32 42
Cuts at: 4650 4682 1373
Size: 32 2626
Fragments arranged by size:
2626 2266 706 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3279 3525 669
Size: 988 281 1341 246 3079
Fragments arranged by size:
3079 1341 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2492 748
Size: 1744 4191
HpaI GTT'AAC
Cuts at: 3525 3525
Size: 5935
KpnI G_GTAC'C
Cuts at: 3260 3260
Size: 5935
MfeI C'AATT_G
Cuts at: 3534 3534
Size: 5935
MluI A'CGCG_T
Cuts at: 3091 5811 3091
Size: 2720 3215
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5049 5233 1305
Size: 3744 184 2007
Fragments arranged by size:
3744 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4113
Fragments arranged by size:
4113 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2574 2832 3893 4252 4411 518
Size: 648 1408 258 1061 359 159 2042
Fragments arranged by size:
2042 1408 1061 648 359 258 159
MspA1I CmG'CkG
Cuts at: 1606 2487 2757 3351 4028 5176 5421 1606
Size: 881 270 594 677 1148 245 2120
Fragments arranged by size:
2120 1148 881 677 594 270 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4444
Fragments arranged by size:
4444 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5935
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5935
NotI GC'GGCC_GC
Cuts at: 3382 3382
Size: 5935
NsiI A_TGCA'T
Cuts at: 2314 2558 2849 3122 2314
Size: 244 291 273 5127
Fragments arranged by size:
5127 291 273 244
NspI r_CATG'y
Cuts at: 1132 1229 2837 2992 3293 4838 1132
Size: 97 1608 155 301 1545 2229
Fragments arranged by size:
2229 1608 1545 301 155 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5935
PciI A'CATG_T
Cuts at: 4834 4834
Size: 5935
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5935
PmeI GTTT'AAAC
Cuts at: 3239 3239
Size: 5935
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4004 4036 5517 5549 4004
Size: 32 1481 32 4390
Fragments arranged by size:
4390 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5935
PsiI TTA'TAA
Cuts at: 3505 3505
Size: 5935
PspXI vC'TCGA_Gb
Cuts at: 2340 2340
Size: 5935
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3018 3050 3018
Size: 32 5903
PstI C_TGCA'G
Cuts at: 830 2515 3287 830
Size: 1685 772 3478
Fragments arranged by size:
3478 1685 772
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5935
PvuII CAG'CTG
Cuts at: 2757 2757
Size: 5935
SacI G_AGCT'C
Cuts at: 2820 2820
Size: 5935
SalI G'TCGA_C
Cuts at: 1936 3277 1936
Size: 1341 4594
SbfI CC_TGCA'GG
Cuts at: 3287 3287
Size: 5935
ScaI AGT'ACT
Cuts at: 2983 4183 5895 2983
Size: 1200 1712 3023
Fragments arranged by size:
3023 1712 1200
SfcI C'TryA_G
Cuts at: 826 1045 2046 2511 3283 4418 5099 5290 826
Size: 219 1001 465 772 1135 681 191 1471
Fragments arranged by size:
1471 1135 1001 772 681 465 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5935
SmaI CCC'GGG
Cuts at: 1872 3262 1872
Size: 1390 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2340 2635 4039 4940 5202 820
Size: 197 926 397 295 1404 901 262 1553
Fragments arranged by size:
1553 1404 926 901 397 295 262 197
SnaBI TAC'GTA
Cuts at: 493 2717 3726 493
Size: 2224 1009 2702
Fragments arranged by size:
2702 2224 1009
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5935
SphI G_CATG'C
Cuts at: 1132 3293 1132
Size: 2161 3774
SspI AAT'ATT
Cuts at: 5 52 3859 5
Size: 47 3807 2081
Fragments arranged by size:
3807 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5935
StyI C'CwwG_G
Cuts at: 513 1065 2004 3159 3374 513
Size: 552 939 1155 215 3074
Fragments arranged by size:
3074 1155 939 552 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3962 3979 4132 4317 4762 325
Size: 1255 2382 17 153 185 445 1498
Fragments arranged by size:
2382 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2461 2981 4181
Size: 274 80 33 51 1443 484 520 1200
Cuts at: 4181 5893 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1200 520 484 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5895 5927 5895
Size: 32 5903
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5935
XbaI T'CTAG_A
Cuts at: 3271 3271
Size: 5935
XhoI C'TCGA_G
Cuts at: 1943 2340 1943
Size: 397 5538
XmaI C'CCGG_G
Cuts at: 1870 3260 1870
Size: 1390 4545
XmnI GAAnn'nnTTC
Cuts at: 2286 2690 4064 2286
Size: 404 1374 4157
Fragments arranged by size:
4157 1374 404
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3743 276
Size: 53 83 186 936 2209 2468
Fragments arranged by size:
2468 2209 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspXI PsrI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FalI FseI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI