(Circular) (Six-base) MAPSORT of: pFN21AB2260.seq Check: 7646 from: 1 to: 7333 pFN21AB2260 7333 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2257 4483 2257 Size: 2226 5107 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5143 278 Size: 53 83 186 936 3607 2468 Fragments arranged by size: 3607 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3780 4676 1937 Size: 1843 896 4594 Fragments arranged by size: 4594 1843 896 Acc65I G'GTAC_C Cuts at: 4654 4654 Size: 7333 AclI AA'CG_TT Cuts at: 1563 5460 5833 1563 Size: 3897 373 3063 Fragments arranged by size: 3897 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2480 3064 3172 5396 6780 7048 1632 Size: 848 584 108 2224 1384 268 1917 Fragments arranged by size: 2224 1917 1384 848 584 268 108 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7136 AflIII A'CryG_T Cuts at: 1203 4368 6232 7209 1203 Size: 3165 1864 977 1327 Fragments arranged by size: 3165 1864 1327 977 AgeI A'CCGG_T Cuts at: 6164 6164 Size: 7333 AhdI GACnn_n'nnGTC Cuts at: 6062 6062 Size: 7333 AleI CACnn'nnGTG Cuts at: 1166 3771 1166 Size: 2605 4728 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7333 AlwNI CAG_nnn'CTG Cuts at: 2160 2743 5076 6648 2160 Size: 583 2333 1572 2845 Fragments arranged by size: 2845 2333 1572 583 ApaI G_GGCC'C Cuts at: 2475 3069 3693 4072 4101 4420 2475 Size: 594 624 379 29 319 5388 Fragments arranged by size: 5388 624 594 379 319 29 ApaLI G'TGCA_C Cuts at: 3764 5390 6546 3764 Size: 1626 1156 4551 Fragments arranged by size: 4551 1626 1156 ApoI r'AATT_y Cuts at: 1493 4222 4642 4868 5020 7129 1493 Size: 2729 420 226 152 2109 1697 Fragments arranged by size: 2729 2109 1697 420 226 152 AseI AT'TA_AT Cuts at: 160 1032 5887 160 Size: 872 4855 1606 Fragments arranged by size: 4855 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7333 AvaI C'yCGr_G Cuts at: 1870 1943 2373 2508 2997 4658 1870 Size: 73 430 135 489 1661 4545 Fragments arranged by size: 4545 1661 489 430 135 73 AvrII C'CTAG_G Cuts at: 2968 2968 Size: 7333 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3437 3470 3437 Size: 33 7300 BamHI G'GATC_C Cuts at: 4663 5030 4663 Size: 367 6966 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2223 2306 3137 3554 4654 Size: 325 217 627 436 83 831 417 1100 Cuts at: 4654 6109 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1100 831 627 436 417 325 217 83 BanII G_rGCy'C Cuts at: 1391 2475 2550 2890 2955 2998 3069 3210 3248 Size: 1084 75 340 65 43 71 141 38 Cuts at: 3248 3465 3693 4072 4101 4420 4652 1391 Size: 217 228 379 29 319 232 4072 Fragments arranged by size: 4072 1084 379 340 319 232 228 217 141 75 71 65 43 38 29 BbeI G_GCGC'C Cuts at: 3558 3558 Size: 7333 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6362 BbvCI CC'TCA_GC Cuts at: 2834 3344 2834 Size: 510 6823 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5524 5558 1903 Size: 34 3587 34 3678 Fragments arranged by size: 3678 3587 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5225 6435 5225 Size: 1210 6123 BclI T'GATC_A Cuts at: 1548 1548 Size: 7333 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2846 4067 4429 Size: 107 122 71 631 605 1174 1221 362 Cuts at: 4429 4527 5944 136 Size: 98 1417 1525 Fragments arranged by size: 1525 1417 1221 1174 631 605 362 122 107 98 71 BglII A'GATC_T Cuts at: 3793 3858 7328 3793 Size: 65 3470 3798 Fragments arranged by size: 3798 3470 65 BlpI GC'TnA_GC Cuts at: 4750 4750 Size: 7333 Bme1580I G_kGCm'C Cuts at: 1790 2228 2309 2475 3015 3069 3693 3768 4039 Size: 438 81 166 540 54 624 75 271 Cuts at: 4039 4072 4101 4420 5394 6550 1790 Size: 33 29 319 974 1156 2573 Fragments arranged by size: 2573 1156 974 624 540 438 319 271 166 81 75 54 33 29 BmgBI CAC'GTC Cuts at: 1318 4369 1318 Size: 3051 4282 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3140 5064 6022 Size: 416 53 83 399 24 1723 1924 958 Cuts at: 6022 442 Size: 1753 Fragments arranged by size: 1924 1753 1723 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7333 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2443 2443 Size: 7333 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2584 2779 3224 4067 4576 5993 1239 Size: 1345 195 445 843 509 1417 2579 Fragments arranged by size: 2579 1417 1345 843 509 445 195 Bpu10I CC'TnA_GC Cuts at: 2834 3344 2834 Size: 510 6823 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3774 3914 5458 6323 6621 3774 Size: 140 1544 865 298 4486 Fragments arranged by size: 4486 1544 865 298 140 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5996 882 Size: 617 4497 2219 Fragments arranged by size: 4497 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5124 493 Size: 711 3920 2702 Fragments arranged by size: 3920 2702 711 BsaBI GATnn'nnATC Cuts at: 7327 7327 Size: 7333 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3555 3630 5140 5522 Size: 53 83 186 936 2022 75 1510 382 Cuts at: 5522 6157 275 Size: 635 1451 Fragments arranged by size: 2022 1510 1451 936 635 382 186 83 75 53 BsaWI w'CCGG_w Cuts at: 2121 5766 6164 6438 6585 6996 2121 Size: 3645 398 274 147 411 2458 Fragments arranged by size: 3645 2458 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2243 2273 2777 2801 2807 Size: 30 165 30 840 30 504 24 6 Cuts at: 2807 2831 2837 1178 Size: 24 6 5674 Fragments arranged by size: 5674 840 504 165 30 30 30 24 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2267 2270 3332 3389 1186 Size: 1081 3 1062 57 5130 Fragments arranged by size: 5130 1081 1062 57 3 BseYI C'CCAG_C Cuts at: 2409 2881 3955 4271 4419 4590 6536 7032 2409 Size: 472 1074 316 148 171 1946 496 2710 Fragments arranged by size: 2710 1946 1074 496 472 316 171 148 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2270 3311 3645 3989 4310 1163 Size: 573 534 1041 334 344 321 4186 Fragments arranged by size: 4186 1041 573 534 344 334 321 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3025 3252 4511 4783 5544 6572 Size: 275 186 1024 227 1259 272 761 1028 Cuts at: 6572 1540 Size: 2301 Fragments arranged by size: 2301 1259 1028 1024 761 275 272 227 186 BsiHKAI G_wGCw'C Cuts at: 2550 3768 5394 5479 6550 2550 Size: 1218 1626 85 1071 3333 Fragments arranged by size: 3333 1626 1218 1071 85 BsmI GAATG_Cn' Cuts at: 3889 4844 4937 3889 Size: 955 93 6285 Fragments arranged by size: 6285 955 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2228 2309 2475 2550 2890 2955 2998 Size: 399 438 81 166 75 340 65 43 Cuts at: 2998 3015 3069 3210 3248 3465 3693 3768 4039 Size: 17 54 141 38 217 228 75 271 Cuts at: 4039 4072 4101 4420 4652 5394 5479 6550 1391 Size: 33 29 319 232 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 438 399 340 319 271 232 228 217 166 141 85 81 75 75 65 54 43 38 33 29 17 BspEI T'CCGG_A Cuts at: 6996 6996 Size: 7333 BspHI T'CATG_A Cuts at: 2523 5222 6952 2523 Size: 2699 1730 2904 Fragments arranged by size: 2904 2699 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2181 2257 2845 4438 4483 4688 844 Size: 1337 76 588 1593 45 205 3489 Fragments arranged by size: 3489 1593 1337 588 205 76 45 BsrBI CCG'CTC Cuts at: 1394 1823 5220 1394 Size: 429 3397 3507 Fragments arranged by size: 3507 3397 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2440 3303 5828 6002 65 Size: 1183 1192 863 2525 174 1396 Fragments arranged by size: 2525 1396 1192 1183 863 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2358 2829 4488 5977 6164 1700 Size: 110 548 471 1659 1489 187 2869 Fragments arranged by size: 2869 1659 1489 548 471 187 110 BsrGI T'GTAC_A Cuts at: 96 4180 96 Size: 4084 3249 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7333 BssSI C'ACGA_G Cuts at: 3471 5393 6405 3471 Size: 1922 1012 4399 Fragments arranged by size: 4399 1922 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4616 794 Size: 3822 3511 BstBI TT'CG_AA Cuts at: 6137 6137 Size: 7333 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7333 BstXI CCAn_nnnn'nTGG Cuts at: 3156 4427 3156 Size: 1271 6062 BstYI r'GATC_y Cuts at: 1972 2272 3793 3858 4663 5030 5415 5432 6884 Size: 300 1521 65 805 367 385 17 1452 Cuts at: 6884 6970 6982 7328 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1521 1452 805 385 367 346 300 86 65 17 12 Bsu36I CC'TnA_GG Cuts at: 2445 2445 Size: 7333 BtgI C'CryG_G Cuts at: 513 1065 2004 2478 2661 2934 3225 513 Size: 552 939 474 183 273 291 4621 Fragments arranged by size: 4621 939 552 474 291 273 183 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6594 Fragments arranged by size: 6594 683 56 BtsI GCAGTG_nn' Cuts at: 2172 2209 3050 3662 4308 4849 5643 5663 2172 Size: 37 841 612 646 541 794 20 3842 Fragments arranged by size: 3842 841 794 646 612 541 37 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7333 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7298 DraI TTT'AAA Cuts at: 4637 4637 Size: 7333 DraIII CAC_nnn'GTG Cuts at: 2293 2293 Size: 7333 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4448 6340 809 Size: 507 3132 1892 1802 Fragments arranged by size: 3132 1892 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2753 3022 3161 Size: 54 1425 50 275 18 923 269 139 Cuts at: 3161 3228 3276 3866 4164 4194 4508 4780 5669 Size: 67 48 590 298 30 314 272 889 Cuts at: 5669 7298 8 Size: 1629 43 Fragments arranged by size: 1629 1425 923 889 590 314 298 275 272 269 139 67 54 50 48 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 3022 4508 4780 1537 Size: 275 1210 1486 272 4090 Fragments arranged by size: 4090 1486 1210 275 272 EarI CTCTTCn'nnn_ Cuts at: 1353 2726 3083 3366 5263 1353 Size: 1373 357 283 1897 3423 Fragments arranged by size: 3423 1897 1373 357 283 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2682 3471 5920 6294 6440 387 Size: 1064 138 1093 789 2449 374 146 1280 Fragments arranged by size: 2449 1280 1093 1064 789 374 146 138 EcoICRI GAG'CTC Cuts at: 2548 2548 Size: 7333 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2480 2584 2779 3064 3172 3224 4067 Size: 393 848 104 195 285 108 52 843 Cuts at: 4067 4576 5396 5993 6780 7048 1239 Size: 509 820 597 787 268 1524 Fragments arranged by size: 1524 848 843 820 787 597 509 393 285 268 195 108 104 52 EcoNI CCTnn'n_nnAGG Cuts at: 2336 3347 3773 3905 4394 4610 2336 Size: 1011 426 132 489 216 5059 Fragments arranged by size: 5059 1011 489 426 216 132 EcoO109I rG'GnC_Cy Cuts at: 2017 2217 2329 2440 2464 2472 2838 2986 3065 Size: 200 112 111 24 8 366 148 79 Cuts at: 3065 3066 3434 3690 3934 4068 4069 4097 4189 Size: 1 368 256 244 134 1 28 92 Cuts at: 4189 4416 4528 2017 Size: 227 112 4822 Fragments arranged by size: 4822 368 366 256 244 227 200 148 134 112 112 111 92 79 28 24 8 1 1 EcoRI G'AATT_C Cuts at: 4642 5020 4642 Size: 378 6955 FspI TGC'GCA Cuts at: 4092 5839 4092 Size: 1747 5586 HaeII r_GCGC'y Cuts at: 1678 3558 6480 1678 Size: 1880 2922 2531 Fragments arranged by size: 2922 2531 1880 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2006 2038 2518 2550 3705 Size: 32 135 32 434 32 480 32 1155 Cuts at: 3705 3737 5974 6006 6048 6080 1373 Size: 32 2237 32 42 32 2626 Fragments arranged by size: 2626 2237 1155 480 434 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3781 4677 4923 669 Size: 988 281 1843 896 246 3079 Fragments arranged by size: 3079 1843 988 896 281 246 HindIII A'AGCT_T Cuts at: 748 3737 748 Size: 2989 4344 HpaI GTT'AAC Cuts at: 4923 4923 Size: 7333 KasI G'GCGC_C Cuts at: 3554 3554 Size: 7333 KpnI G_GTAC'C Cuts at: 4658 4658 Size: 7333 MfeI C'AATT_G Cuts at: 4932 4932 Size: 7333 MluI A'CGCG_T Cuts at: 7209 7209 Size: 7333 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2175 3348 6447 6631 1305 Size: 870 1173 3099 184 2007 Fragments arranged by size: 3099 2007 1173 870 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2755 3230 3278 4166 4196 Size: 54 1425 343 923 475 48 888 30 Cuts at: 4196 10 Size: 3147 Fragments arranged by size: 3147 1425 923 888 475 343 54 48 30 MslI CAynn'nnrTG Cuts at: 518 1166 3771 4340 5291 5650 5809 518 Size: 648 2605 569 951 359 159 2042 Fragments arranged by size: 2605 2042 951 648 569 359 159 MspA1I CmG'CkG Cuts at: 1606 2388 2409 2695 4045 4317 4594 4749 5426 Size: 782 21 286 1350 272 277 155 677 Cuts at: 5426 6574 6819 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1350 1148 782 677 286 277 272 245 155 21 NaeI GCC'GGC Cuts at: 4490 4490 Size: 7333 NarI GG'CG_CC Cuts at: 3555 3555 Size: 7333 NcoI C'CATG_G Cuts at: 513 1065 2004 2478 2934 513 Size: 552 939 474 456 4912 Fragments arranged by size: 4912 939 552 474 456 NdeI CA'TA_TG Cuts at: 387 387 Size: 7333 NgoMIV G'CCGG_C Cuts at: 4488 4488 Size: 7333 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7333 NotI GC'GGCC_GC Cuts at: 4780 4780 Size: 7333 NspI r_CATG'y Cuts at: 1132 1229 2116 2324 2875 4130 4156 4691 6236 Size: 97 887 208 551 1255 26 535 1545 Cuts at: 6236 1132 Size: 2229 Fragments arranged by size: 2229 1545 1255 887 551 535 208 97 26 PasI CC'CwG_GG Cuts at: 1348 2107 2311 3401 3671 4073 1348 Size: 759 204 1090 270 402 4608 Fragments arranged by size: 4608 1090 759 402 270 204 PciI A'CATG_T Cuts at: 6232 6232 Size: 7333 PflMI CCAn_nnn'nTGG Cuts at: 1108 2160 2940 4045 4317 1108 Size: 1052 780 1105 272 4124 Fragments arranged by size: 4124 1105 1052 780 272 PfoI T'CCnGG_A Cuts at: 2348 4476 2348 Size: 2128 5205 PmeI GTTT'AAAC Cuts at: 4637 4637 Size: 7333 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3104 3136 5402 5434 6915 6947 3104 Size: 32 2266 32 1481 32 3490 Fragments arranged by size: 3490 2266 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2440 2464 2986 3434 3934 4189 2440 Size: 24 522 448 500 255 5584 Fragments arranged by size: 5584 522 500 448 255 24 PshAI GACnn'nnGTC Cuts at: 1613 2578 1613 Size: 965 6368 PsiI TTA'TAA Cuts at: 4903 4903 Size: 7333 PspOMI G'GGCC_C Cuts at: 2471 3065 3689 4068 4097 4416 2471 Size: 594 624 379 29 319 5388 Fragments arranged by size: 5388 624 594 379 319 29 PstI C_TGCA'G Cuts at: 830 2676 3045 3627 4685 830 Size: 1846 369 582 1058 3478 Fragments arranged by size: 3478 1846 1058 582 369 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7333 PvuII CAG'CTG Cuts at: 2695 4045 4594 2695 Size: 1350 549 5434 Fragments arranged by size: 5434 1350 549 SacI G_AGCT'C Cuts at: 2550 2550 Size: 7333 SalI G'TCGA_C Cuts at: 1936 3779 4675 1936 Size: 1843 896 4594 Fragments arranged by size: 4594 1843 896 SanDI GG'GwC_CC Cuts at: 2986 2986 Size: 7333 SbfI CC_TGCA'GG Cuts at: 3627 4685 3627 Size: 1058 6275 ScaI AGT'ACT Cuts at: 2261 5581 7293 2261 Size: 3320 1712 2301 Fragments arranged by size: 3320 2301 1712 SexAI A'CCwGG_T Cuts at: 2289 2289 Size: 7333 SfcI C'TryA_G Cuts at: 826 1045 2672 3041 3520 3623 4411 4681 5816 Size: 219 1627 369 479 103 788 270 1135 Cuts at: 5816 6497 6688 826 Size: 681 191 1471 Fragments arranged by size: 1627 1471 1135 788 681 479 369 270 219 191 103 SfoI GGC'GCC Cuts at: 3556 3556 Size: 7333 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7333 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7333 SmaI CCC'GGG Cuts at: 1872 2375 2510 2999 4660 1872 Size: 503 135 489 1661 4545 Fragments arranged by size: 4545 1661 503 489 135 SmlI C'TyrA_G Cuts at: 820 1017 1943 3789 3929 5437 6338 6600 820 Size: 197 926 1846 140 1508 901 262 1553 Fragments arranged by size: 1846 1553 1508 926 901 262 197 140 SnaBI TAC'GTA Cuts at: 493 5124 493 Size: 4631 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7333 SphI G_CATG'C Cuts at: 1132 2116 2324 4156 4691 1132 Size: 984 208 1832 535 3774 Fragments arranged by size: 3774 1832 984 535 208 SrfI GCCC'GGGC Cuts at: 2999 2999 Size: 7333 SspI AAT'ATT Cuts at: 5 52 5257 5 Size: 47 5205 2081 Fragments arranged by size: 5205 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7333 StyI C'CwwG_G Cuts at: 513 1065 2004 2247 2467 2478 2934 2968 3016 Size: 552 939 243 220 11 456 34 48 Cuts at: 3016 3231 4772 513 Size: 215 1541 3074 Fragments arranged by size: 3074 1541 939 552 456 243 220 215 48 34 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2324 5360 5377 5530 5715 6160 325 Size: 1255 744 3036 17 153 185 445 1498 Fragments arranged by size: 3036 1498 1255 744 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2259 4180 5579 Size: 274 80 33 51 1443 282 1921 1399 Cuts at: 5579 7291 96 Size: 1712 138 Fragments arranged by size: 1921 1712 1443 1399 282 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2646 2678 2777 2809 7293 7325 2646 Size: 32 99 32 4484 32 2654 Fragments arranged by size: 4484 2654 99 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2519 1316 Size: 1203 6130 XbaI T'CTAG_A Cuts at: 4669 4669 Size: 7333 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2699 2803 3156 4318 2699 Size: 104 353 1162 5714 Fragments arranged by size: 5714 1162 353 104 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7333 XmaI C'CCGG_G Cuts at: 1870 2373 2508 2997 4658 1870 Size: 503 135 489 1661 4545 Fragments arranged by size: 4545 1661 503 489 135 XmnI GAAnn'nnTTC Cuts at: 3896 5462 3896 Size: 1566 5767 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5141 276 Size: 53 83 186 936 3607 2468 Fragments arranged by size: 3607 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BfrBI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SfiI SwaI