(Circular) (Six-base) MAPSORT of: pFN21AB1891.seq Check: 4227 from: 1 to: 9094 pFN21AB1891 9094 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6904 278 Size: 53 83 186 936 5368 2468 Fragments arranged by size: 5368 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2972 3285 4613 6315 6374 6437 1937 Size: 1035 313 1328 1702 59 63 4594 Fragments arranged by size: 4594 1702 1328 1035 313 63 59 Acc65I G'GTAC_C Cuts at: 6415 6415 Size: 9094 AclI AA'CG_TT Cuts at: 1563 7221 7594 1563 Size: 5658 373 3063 Fragments arranged by size: 5658 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2545 3069 3945 4218 5496 7157 8541 8809 Size: 913 524 876 273 1278 1661 1384 268 Cuts at: 8809 1632 Size: 1917 Fragments arranged by size: 1917 1661 1384 1278 913 876 524 273 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8897 AflIII A'CryG_T Cuts at: 1203 2975 3659 3947 7993 8970 1203 Size: 1772 684 288 4046 977 1327 Fragments arranged by size: 4046 1772 1327 977 684 288 AgeI A'CCGG_T Cuts at: 7925 7925 Size: 9094 AhdI GACnn_n'nnGTC Cuts at: 7823 7823 Size: 9094 AleI CACnn'nnGTG Cuts at: 1166 5783 1166 Size: 4617 4477 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9094 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2797 2829 5101 5133 2797 Size: 32 2272 32 6758 Fragments arranged by size: 6758 2272 32 32 AlwNI CAG_nnn'CTG Cuts at: 3242 6837 8409 3242 Size: 3595 1572 3927 Fragments arranged by size: 3927 3595 1572 ApaLI G'TGCA_C Cuts at: 7151 8307 7151 Size: 1156 7938 ApoI r'AATT_y Cuts at: 1493 2273 2383 2566 2642 4243 5035 5164 5173 Size: 780 110 183 76 1601 792 129 9 Cuts at: 5173 5320 5506 6138 6277 6403 6629 6781 8890 Size: 147 186 632 139 126 226 152 2109 Cuts at: 8890 1493 Size: 1697 Fragments arranged by size: 2109 1697 1601 792 780 632 226 186 183 152 147 139 129 126 110 76 9 AseI AT'TA_AT Cuts at: 160 1032 7648 160 Size: 872 6616 1606 Fragments arranged by size: 6616 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9094 AvaI C'yCGr_G Cuts at: 1870 1943 3488 6419 1870 Size: 73 1545 2931 4545 Fragments arranged by size: 4545 2931 1545 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5802 5835 5802 Size: 33 9061 BamHI G'GATC_C Cuts at: 6424 6791 6424 Size: 367 8727 BanI G'GyrC_C Cuts at: 618 943 1160 1787 6415 7870 618 Size: 325 217 627 4628 1455 1842 Fragments arranged by size: 4628 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3182 3830 3893 6413 1391 Size: 1791 648 63 2520 4072 Fragments arranged by size: 4072 2520 1791 648 63 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2061 2182 4272 6125 928 Size: 971 162 121 2090 1853 3897 Fragments arranged by size: 3897 2090 1853 971 162 121 BbvCI CC'TCA_GC Cuts at: 5757 5757 Size: 9094 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6096 6130 7285 7319 1903 Size: 34 4159 34 1155 34 3678 Fragments arranged by size: 4159 3678 1155 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2454 6986 8196 2454 Size: 4532 1210 3352 Fragments arranged by size: 4532 3352 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 9094 BfrBI ATG'CAT Cuts at: 2867 2867 Size: 9094 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7705 136 Size: 107 122 71 631 605 6033 1525 Fragments arranged by size: 6033 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2425 3445 4082 4376 4634 4682 4811 5420 9089 Size: 1020 637 294 258 48 129 609 3669 Cuts at: 9089 2425 Size: 2430 Fragments arranged by size: 3669 2430 1020 637 609 294 258 129 48 BlpI GC'TnA_GC Cuts at: 6511 6511 Size: 9094 Bme1580I G_kGCm'C Cuts at: 1790 5790 7155 8311 1790 Size: 4000 1365 1156 2573 Fragments arranged by size: 4000 2573 1365 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9094 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4441 4482 6825 Size: 416 53 83 399 24 3024 41 2343 Cuts at: 6825 7783 442 Size: 958 1753 Fragments arranged by size: 3024 2343 1753 958 416 399 83 53 41 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9094 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4605 5807 4605 Size: 1202 7892 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2625 7754 1239 Size: 1386 5129 2579 Fragments arranged by size: 5129 2579 1386 Bpu10I CC'TnA_GC Cuts at: 5757 5757 Size: 9094 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2865 3426 4816 7219 8084 8382 2865 Size: 561 1390 2403 865 298 3577 Fragments arranged by size: 3577 2403 1390 865 561 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2374 4605 7757 882 Size: 617 875 2231 3152 2219 Fragments arranged by size: 3152 2231 2219 875 617 BsaAI yAC'GTr Cuts at: 493 1204 2978 6885 493 Size: 711 1774 3907 2702 Fragments arranged by size: 3907 2702 1774 711 BsaBI GATnn'nnATC Cuts at: 3421 4639 5431 5726 9088 3421 Size: 1218 792 295 3362 3427 Fragments arranged by size: 3427 3362 1218 792 295 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6901 7283 7918 275 Size: 53 83 186 936 5368 382 635 1451 Fragments arranged by size: 5368 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7527 7925 8199 8346 8757 7527 Size: 398 274 147 411 7864 Fragments arranged by size: 7864 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8869 Fragments arranged by size: 8869 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3827 4783 5558 1186 Size: 2641 956 775 4722 Fragments arranged by size: 4722 2641 956 775 BseYI C'CCAG_C Cuts at: 5445 8297 8793 5445 Size: 2852 496 5746 Fragments arranged by size: 5746 2852 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3329 1163 Size: 573 1593 6928 Fragments arranged by size: 6928 1593 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6544 7305 8333 1540 Size: 275 186 4543 761 1028 2301 Fragments arranged by size: 4543 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3182 3273 3442 7155 7240 8311 3182 Size: 91 169 3713 85 1071 3965 Fragments arranged by size: 3965 3713 1071 169 91 85 BsmI GAATG_Cn' Cuts at: 5668 6605 6698 5668 Size: 937 93 8064 Fragments arranged by size: 8064 937 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3182 3273 3442 3830 3893 5790 6413 Size: 399 1392 91 169 388 63 1897 623 Cuts at: 6413 7155 7240 8311 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1897 1392 1071 742 623 399 388 169 91 85 63 BspEI T'CCGG_A Cuts at: 8757 8757 Size: 9094 BspHI T'CATG_A Cuts at: 2377 6983 8713 2377 Size: 4606 1730 2758 Fragments arranged by size: 4606 2758 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2953 5788 6449 844 Size: 2109 2835 661 3489 Fragments arranged by size: 3489 2835 2109 661 BsrBI CCG'CTC Cuts at: 1394 1823 6981 1394 Size: 429 5158 3507 Fragments arranged by size: 5158 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2955 3361 5877 7589 7763 65 Size: 1183 1707 406 2516 1712 174 1396 Fragments arranged by size: 2516 1712 1707 1396 1183 406 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7738 7925 1700 Size: 110 5928 187 2869 Fragments arranged by size: 5928 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2106 2636 3629 5476 96 Size: 2010 530 993 1847 3714 Fragments arranged by size: 3714 2010 1847 993 530 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9094 BssSI C'ACGA_G Cuts at: 4938 7154 8166 4938 Size: 2216 1012 5866 Fragments arranged by size: 5866 2216 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9094 BstBI TT'CG_AA Cuts at: 2835 7898 2835 Size: 5063 4031 BstEII G'GTnAC_C Cuts at: 1184 4407 1184 Size: 3223 5871 BstXI CCAn_nnnn'nTGG Cuts at: 3593 3593 Size: 9094 BstYI r'GATC_y Cuts at: 1972 2425 3014 3416 3445 4082 4376 4634 4682 Size: 453 589 402 29 637 294 258 48 Cuts at: 4682 4811 5420 6424 6791 7176 7193 8645 8731 Size: 129 609 1004 367 385 17 1452 86 Cuts at: 8731 8743 9089 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1004 637 609 589 453 402 385 367 346 294 258 129 86 48 29 17 12 BstZ17I GTA'TAC Cuts at: 4614 4614 Size: 9094 BtgI C'CryG_G Cuts at: 513 1065 5215 5236 513 Size: 552 4150 21 4371 Fragments arranged by size: 4371 4150 552 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8355 Fragments arranged by size: 8355 683 56 BtsI GCAGTG_nn' Cuts at: 2271 4186 6099 6610 7404 7424 2271 Size: 1915 1913 511 794 20 3941 Fragments arranged by size: 3941 1915 1913 794 511 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9094 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9059 DraI TTT'AAA Cuts at: 2272 6398 2272 Size: 4126 4968 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8101 809 Size: 507 6785 1802 Fragments arranged by size: 6785 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 6171 6541 7430 Size: 54 1425 50 275 18 4341 370 889 Cuts at: 7430 9059 8 Size: 1629 43 Fragments arranged by size: 4341 1629 1425 889 370 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6541 1537 Size: 275 4729 4090 Fragments arranged by size: 4729 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2678 3088 3133 4459 4651 7024 1353 Size: 1325 410 45 1326 192 2373 3423 Fragments arranged by size: 3423 2373 1326 1325 410 192 45 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7681 8055 8201 387 Size: 1064 138 6092 374 146 1280 Fragments arranged by size: 6092 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3180 3180 Size: 9094 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2545 2625 3069 3945 4218 5496 7157 Size: 393 913 80 444 876 273 1278 1661 Cuts at: 7157 7754 8541 8809 1239 Size: 597 787 268 1524 Fragments arranged by size: 1661 1524 1278 913 876 787 597 444 393 273 268 80 EcoNI CCTnn'n_nnAGG Cuts at: 5795 5795 Size: 9094 EcoO109I rG'GnC_Cy Cuts at: 4728 4824 4728 Size: 96 8998 EcoRI G'AATT_C Cuts at: 5320 6403 6781 5320 Size: 1083 378 7633 Fragments arranged by size: 7633 1083 378 EcoRV GAT'ATC Cuts at: 3591 6202 3591 Size: 2611 6483 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4680 4680 Size: 9094 FspI TGC'GCA Cuts at: 7600 7600 Size: 9094 HaeII r_GCGC'y Cuts at: 1678 8241 1678 Size: 6563 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2536 2568 3575 3607 3863 Size: 32 135 32 964 32 1007 32 256 Cuts at: 3863 3895 4060 4092 4874 4906 5537 5569 7735 Size: 32 165 32 782 32 631 32 2166 Cuts at: 7735 7767 7809 7841 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2166 1007 964 782 631 256 165 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5872 6438 6684 669 Size: 988 281 3934 566 246 3079 Fragments arranged by size: 3934 3079 988 566 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9094 HpaI GTT'AAC Cuts at: 6684 6684 Size: 9094 KpnI G_GTAC'C Cuts at: 6419 6419 Size: 9094 MfeI C'AATT_G Cuts at: 3902 5068 5903 6693 3902 Size: 1166 835 790 6303 Fragments arranged by size: 6303 1166 835 790 MluI A'CGCG_T Cuts at: 8970 8970 Size: 9094 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2840 4907 5755 5993 8208 8392 1305 Size: 1535 2067 848 238 2215 184 2007 Fragments arranged by size: 2215 2067 2007 1535 848 238 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 6173 10 Size: 54 1425 343 4341 2931 Fragments arranged by size: 4341 2931 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4131 5019 5055 5783 7052 7411 7570 Size: 648 2965 888 36 728 1269 359 159 Cuts at: 7570 518 Size: 2042 Fragments arranged by size: 2965 2042 1269 888 728 648 359 159 36 MspA1I CmG'CkG Cuts at: 1606 2320 3089 5445 6510 7187 8335 8580 1606 Size: 714 769 2356 1065 677 1148 245 2120 Fragments arranged by size: 2356 2120 1148 1065 769 714 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 8542 NdeI CA'TA_TG Cuts at: 387 387 Size: 9094 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9094 NotI GC'GGCC_GC Cuts at: 6541 6541 Size: 9094 NsiI A_TGCA'T Cuts at: 2869 2869 Size: 9094 NspI r_CATG'y Cuts at: 1132 1229 3663 3917 3951 6452 7997 1132 Size: 97 2434 254 34 2501 1545 2229 Fragments arranged by size: 2501 2434 2229 1545 254 97 34 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9094 PciI A'CATG_T Cuts at: 3659 3947 7993 3659 Size: 288 4046 4760 Fragments arranged by size: 4760 4046 288 PflMI CCAn_nnn'nTGG Cuts at: 1108 4797 5525 6014 1108 Size: 3689 728 489 4188 Fragments arranged by size: 4188 3689 728 489 PmeI GTTT'AAAC Cuts at: 6398 6398 Size: 9094 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7163 7195 8676 8708 7163 Size: 32 1481 32 7549 Fragments arranged by size: 7549 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4728 4824 4728 Size: 96 8998 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9094 PsiI TTA'TAA Cuts at: 6664 6664 Size: 9094 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3618 3650 5905 5937 3618 Size: 32 2255 32 6775 Fragments arranged by size: 6775 2255 32 32 PstI C_TGCA'G Cuts at: 830 6446 830 Size: 5616 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9094 PvuII CAG'CTG Cuts at: 2320 3089 5445 2320 Size: 769 2356 5969 Fragments arranged by size: 5969 2356 769 SacI G_AGCT'C Cuts at: 3182 3182 Size: 9094 SalI G'TCGA_C Cuts at: 1936 6436 1936 Size: 4500 4594 SbfI CC_TGCA'GG Cuts at: 6446 6446 Size: 9094 ScaI AGT'ACT Cuts at: 7342 9054 7342 Size: 1712 7382 SfcI C'TryA_G Cuts at: 826 1045 2623 3183 4086 6442 7577 8258 8449 Size: 219 1578 560 903 2356 1135 681 191 Cuts at: 8449 826 Size: 1471 Fragments arranged by size: 2356 1578 1471 1135 903 681 560 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9094 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9094 SmaI CCC'GGG Cuts at: 1872 6421 1872 Size: 4549 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2880 3441 3488 4831 7198 8099 Size: 197 926 937 561 47 1343 2367 901 Cuts at: 8099 8361 820 Size: 262 1553 Fragments arranged by size: 2367 1553 1343 937 926 901 561 262 197 47 SnaBI TAC'GTA Cuts at: 493 6885 493 Size: 6392 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9094 SphI G_CATG'C Cuts at: 1132 6452 1132 Size: 5320 3774 SspI AAT'ATT Cuts at: 5 52 3547 3982 6329 7018 5 Size: 47 3495 435 2347 689 2081 Fragments arranged by size: 3495 2347 2081 689 435 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9094 StyI C'CwwG_G Cuts at: 513 1065 6207 6533 513 Size: 552 5142 326 3074 Fragments arranged by size: 5142 3074 552 326 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3027 4085 4426 4587 5693 7121 7138 Size: 1255 1447 1058 341 161 1106 1428 17 Cuts at: 7138 7291 7476 7921 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1447 1428 1255 1106 1058 445 341 185 161 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2106 2546 2636 Size: 274 80 33 51 1443 129 440 90 Cuts at: 2636 3302 3629 5476 5948 7340 9052 96 Size: 666 327 1847 472 1392 1712 138 Fragments arranged by size: 1847 1712 1443 1392 666 472 440 327 274 138 129 90 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 9054 9086 9054 Size: 32 9062 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9094 XbaI T'CTAG_A Cuts at: 4379 4667 5017 6430 4379 Size: 288 350 1413 7043 Fragments arranged by size: 7043 1413 350 288 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4401 5526 5597 4401 Size: 1125 71 7898 Fragments arranged by size: 7898 1125 71 XhoI C'TCGA_G Cuts at: 1943 3488 1943 Size: 1545 7549 XmaI C'CCGG_G Cuts at: 1870 6419 1870 Size: 4549 4545 XmnI GAAnn'nnTTC Cuts at: 2775 3546 4116 5509 5687 7223 2775 Size: 771 570 1393 178 1536 4646 Fragments arranged by size: 4646 1536 1393 771 570 178 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6902 276 Size: 53 83 186 936 5368 2468 Fragments arranged by size: 5368 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BsiWI BsmBI Bsu36I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI