Restriction map for
pFN21AB1226
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# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AhdI Alw44I Aor51HI ApaLI AscI
AspEI AviII BbrPI BciVI BfrI
BglII BseAI BseRI BsiWI BsmBI
BspEI BspHI BspLU11I Bst1107I BstBI
BstZ17I CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoRI FseI
FspI HpaI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NspV PacI PaeR7I PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
XhoI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 2867 GGTACC G^GTACC
AcsI 1807 GAATTT [GA]^AATT[CT]
AcyI 912 GGCGCC G[GA]^CG[CT]C
AgeI 1925 ACCGGT A^CCGGT
AocI 1405 CCTGAGG CC^T[ACGT]AGG
ApaI 31 GGGCCC GGGCC^C
ApoI 1807 GAATTT [GA]^AATT[CT]
AseI 3160 ATTAAT AT^TAAT
AsnI 3160 ATTAAT AT^TAAT
Asp718I 2867 GGTACC G^GTACC
AspI 1261 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 285 CCTAGG C^CTAGG
BamHI 3475 GGATCC G^GATCC
BbeI 912 GGCGCC GGCGC^C
BcgI 3443 CGAGGAAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 616 GCCAATGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 285 CCTAGG C^CTAGG
BlpI 66 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 66 GCTGAGC GC^T[ACGT]AGC
BsaAI 3014 TACGTG [CT]AC^GT[GA]
BsaHI 912 GGCGCC G[GA]^CG[CT]C
BsaI 813 GGTCTC GGTCTC(1/5)
BsiEI 308 CGACCG CG[GA][CT]^CG
BsmI 2735 GCATTC GAATGC(1/-1)
BspDI 3127 ATCGAT AT^CGAT
BspMI 1392 GCAGGT ACCTGC(4/8)
BssHII 3584 GCGCGC G^CGCGC
BssSI 3441 CACGAG CACGAG(-5/-1)
BstAPI 2106 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1405 CCTGAGG CC^T[ACGT]AGG
CelII 66 GCTGAGC GC^T[ACGT]AGC
ClaI 3127 ATCGAT AT^CGAT
EarI 2995 GAAGAG CTCTTC(1/4)
Eco81I 1405 CCTGAGG CC^T[ACGT]AGG
EcoNI 280 CCTCCCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1239 GATATC GAT^ATC
EcoT22I 1678 ATGCAT ATGCA^T
HgaI 3498 GACGC GACGC(5/10)
Hin1I 912 GGCGCC G[GA]^CG[CT]C
KasI 912 GGCGCC G^GCGCC
KpnI 2867 GGTACC GGTAC^C
Ksp632I 2995 GAAGAG CTCTTC(1/4)
NarI 912 GGCGCC GG^CGCC
NcoI 2936 CCATGG C^CATGG
NsiI 1678 ATGCAT ATGCA^T
NspI 1769 ACATGC [GA]CATG^[CT]
PflFI 1261 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1925 ACCGGT A^CCGGT
PshAI 784 GACGAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3160 ATTAAT AT^TAAT
SacI 316 GAGCTC GAGCT^C
SfoI 912 GGCGCC GGC^GCC
SmaI 2670 CCCGGG CCC^GGG
Tth111I 1261 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2670 CCCGGG C^CCGGG
XmaI 2670 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 1736 ACGTGT A^C[GA][CT]GT
1955 ACACGT
Asp700I 2790 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3202 GAATACCTTC
AvaI 2425 CCCGAG C^[CT]CG[GA]G
2670 CCCGGG
BalI 2763 TGGCCA TGG^CCA
3276 TGGCCA
BclI 2480 TGATCA T^GATCA
3188 TGATCA
BfaI 286 CTAG C^TAG
1967 CTAG
BsaWI 1925 ACCGGT [AT]^CCGG[AT]
2798 TCCGGT
BsoBI 2425 CCCGAG C^[CT]CG[GA]G
2670 CCCGGG
BsrBI 69 GAGCGG CCGCTC(-3/-3)
3079 GAGCGG
DraI 520 TTTAAA TTT^AAA
1363 TTTAAA
DrdI 1560 GACTCTACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2046 GACTATCAAGTC
FbaI 2480 TGATCA T^GATCA
3188 TGATCA
HaeII 912 GGCGCC [GA]GCGC^[CT]
3084 GGCGCT
HindIII 2634 AAGCTT A^AGCTT
3226 AAGCTT
KspI 35 CCGCGG CCGC^GG
50 CCGCGG
MaeI 286 CTAG C^TAG
1967 CTAG
MluNI 2763 TGGCCA TGG^CCA
3276 TGGCCA
MscI 2763 TGGCCA TGG^CCA
3276 TGGCCA
NaeI 81 GCCGGC GCC^GGC
3566 GCCGGC
NgoMIV 81 GCCGGC G^CCGGC
3566 GCCGGC
PflMI 760 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
972 CCACGTCATGG
PpuMI 401 AGGTCCT [GA]G^G[AT]CC[CT]
426 GGGTCCT
PvuII 1195 CAGCTG CAG^CTG
2128 CAGCTG
SacII 35 CCGCGG CCGC^GG
50 CCGCGG
StuI 2397 AGGCCT AGG^CCT
2612 AGGCCT
Van91I 760 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
972 CCACGTCATGG
XcmI 1995 CCACCATGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2936 CCATGGAAGCGGTGG
XmnI 2790 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3202 GAATACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BmyI
BpmI BpuAI BsaBI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BsrDI BsrFI BsrGI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII