(Circular) (Six-base) MAPSORT of: pFN21AB1226.seq Check: 2290 from: 1 to: 8371 pFN21AB1226 8371 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3388 3388 Size: 8371 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6181 278 Size: 53 83 186 936 4645 2468 Fragments arranged by size: 4645 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5714 1937 Size: 3777 4594 Acc65I G'GTAC_C Cuts at: 4872 5692 4872 Size: 820 7551 AclI AA'CG_TT Cuts at: 1563 6498 6871 1563 Size: 4935 373 3063 Fragments arranged by size: 4935 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2294 4121 4874 6434 7818 8086 1632 Size: 662 1827 753 1560 1384 268 1917 Fragments arranged by size: 1917 1827 1560 1384 753 662 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8174 AflIII A'CryG_T Cuts at: 1203 3741 3960 7270 8247 1203 Size: 2538 219 3310 977 1327 Fragments arranged by size: 3310 2538 1327 977 219 AgeI A'CCGG_T Cuts at: 3930 7202 3930 Size: 3272 5099 AhdI GACnn_n'nnGTC Cuts at: 7100 7100 Size: 8371 AleI CACnn'nnGTG Cuts at: 1166 2597 5405 1166 Size: 1431 2808 4132 Fragments arranged by size: 4132 2808 1431 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2615 1184 Size: 1431 6940 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3018 3050 3018 Size: 32 8339 AlwNI CAG_nnn'CTG Cuts at: 2428 2771 3234 3521 3577 4117 4135 4852 5290 Size: 343 463 287 56 540 18 717 438 Cuts at: 5290 5609 6114 7686 2428 Size: 319 505 1572 3113 Fragments arranged by size: 3113 1572 717 540 505 463 438 343 319 287 56 18 ApaI G_GGCC'C Cuts at: 2040 2040 Size: 8371 ApaLI G'TGCA_C Cuts at: 6428 7584 6428 Size: 1156 7215 ApoI r'AATT_y Cuts at: 1493 3812 5680 5906 6058 8167 1493 Size: 2319 1868 226 152 2109 1697 Fragments arranged by size: 2319 2109 1868 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5166 6925 160 Size: 872 4134 1759 1606 Fragments arranged by size: 4134 1759 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8371 AvaI C'yCGr_G Cuts at: 1870 1943 4430 4675 5696 1870 Size: 73 2487 245 1021 4545 Fragments arranged by size: 4545 2487 1021 245 73 AvrII C'CTAG_G Cuts at: 2290 2290 Size: 8371 BamHI G'GATC_C Cuts at: 5480 5701 6068 5480 Size: 221 367 7783 Fragments arranged by size: 7783 367 221 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2917 4511 4872 5692 7147 Size: 325 217 627 1130 1594 361 820 1455 Cuts at: 7147 618 Size: 1842 Fragments arranged by size: 1842 1594 1455 1130 820 627 361 325 217 BanII G_rGCy'C Cuts at: 1391 2040 2325 2691 2970 4766 4926 5690 1391 Size: 649 285 366 279 1796 160 764 4072 Fragments arranged by size: 4072 1796 764 649 366 285 279 160 BbeI G_GCGC'C Cuts at: 2921 2921 Size: 8371 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2371 3957 4053 4899 4989 5663 928 Size: 971 472 1586 96 846 90 674 3636 Fragments arranged by size: 3636 1586 971 846 674 472 96 90 BbvCI CC'TCA_GC Cuts at: 2411 3198 4656 2411 Size: 787 1458 6126 Fragments arranged by size: 6126 1458 787 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5437 5471 6562 6596 1903 Size: 34 50 34 3416 34 1091 34 3678 Fragments arranged by size: 3678 3416 1091 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6263 7473 6263 Size: 1210 7161 BclI T'GATC_A Cuts at: 1548 4485 5193 1548 Size: 2937 708 4726 Fragments arranged by size: 4726 2937 708 BfrBI ATG'CAT Cuts at: 3685 3685 Size: 8371 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2627 6982 136 Size: 107 122 71 631 605 955 4355 1525 Fragments arranged by size: 4355 1525 955 631 605 122 107 71 BglII A'GATC_T Cuts at: 8366 8366 Size: 8371 BlpI GC'TnA_GC Cuts at: 2072 5788 2072 Size: 3716 4655 Bme1580I G_kGCm'C Cuts at: 1790 2040 2221 6432 7588 1790 Size: 250 181 4211 1156 2573 Fragments arranged by size: 4211 2573 1156 250 181 BmgBI CAC'GTC Cuts at: 1318 2143 2980 3742 3963 1318 Size: 825 837 762 221 5726 Fragments arranged by size: 5726 837 825 762 221 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2394 2475 4279 Size: 416 53 83 399 24 977 81 1804 Cuts at: 4279 5362 6102 7060 442 Size: 1083 740 958 1753 Fragments arranged by size: 1804 1753 1083 977 958 740 416 399 83 81 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8371 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4834 4834 Size: 8371 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2559 2806 3590 3737 3752 4302 4565 7031 Size: 1320 247 784 147 15 550 263 2466 Cuts at: 7031 1239 Size: 2579 Fragments arranged by size: 2579 2466 1320 784 550 263 247 147 15 Bpu10I CC'TnA_GC Cuts at: 2411 3198 3578 4656 4760 2411 Size: 787 380 1078 104 6022 Fragments arranged by size: 6022 1078 787 380 104 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3125 3294 5042 5373 6496 7361 7659 3125 Size: 169 1748 331 1123 865 298 3837 Fragments arranged by size: 3837 1748 1123 865 331 298 169 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2824 7034 882 Size: 617 1325 4210 2219 Fragments arranged by size: 4210 2219 1325 617 BsaAI yAC'GTr Cuts at: 493 1204 5021 6162 493 Size: 711 3817 1141 2702 Fragments arranged by size: 3817 2702 1141 711 BsaBI GATnn'nnATC Cuts at: 3248 4385 4454 4862 8365 3248 Size: 1137 69 408 3503 3254 Fragments arranged by size: 3503 3254 1137 408 69 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2918 6178 6560 7195 Size: 53 83 186 936 1385 3260 382 635 Cuts at: 7195 275 Size: 1451 Fragments arranged by size: 3260 1451 1385 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3930 4803 6804 7202 7476 7623 8034 3930 Size: 873 2001 398 274 147 411 4267 Fragments arranged by size: 4267 2001 873 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2433 2463 2804 2834 3018 Size: 30 165 30 1030 30 341 30 184 Cuts at: 3018 3048 4790 4820 1178 Size: 30 1742 30 4729 Fragments arranged by size: 4729 1742 1030 341 184 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 8371 BseYI C'CCAG_C Cuts at: 2032 7574 8070 2032 Size: 5542 496 2333 Fragments arranged by size: 5542 2333 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2335 3416 4130 4238 4517 4856 1163 Size: 573 599 1081 714 108 279 339 4678 Fragments arranged by size: 4678 1081 714 599 573 339 279 108 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2316 5821 6582 7610 1540 Size: 275 186 315 3505 761 1028 2301 Fragments arranged by size: 3505 2301 1028 761 315 275 186 BsiHKAI G_wGCw'C Cuts at: 2325 3153 3657 6432 6517 7588 2325 Size: 828 504 2775 85 1071 3108 Fragments arranged by size: 3108 2775 1071 828 504 85 BsmI GAATG_Cn' Cuts at: 4740 5882 5975 4740 Size: 1142 93 7136 Fragments arranged by size: 7136 1142 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2221 2325 2691 2970 3153 3657 Size: 399 250 181 104 366 279 183 504 Cuts at: 3657 4766 4926 5690 6432 6517 7588 1391 Size: 1109 160 764 742 85 1071 2174 Fragments arranged by size: 2174 1109 1071 764 742 504 399 366 279 250 183 181 160 104 85 BspEI T'CCGG_A Cuts at: 8034 8034 Size: 8371 BspHI T'CATG_A Cuts at: 6260 7990 6260 Size: 1730 6641 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3388 5726 844 Size: 2544 2338 3489 Fragments arranged by size: 3489 2544 2338 BsrBI CCG'CTC Cuts at: 1394 1823 2076 5086 6258 1394 Size: 429 253 3010 1172 3507 Fragments arranged by size: 3507 3010 1172 429 253 BsrDI GCAATG_nn' Cuts at: 65 1248 2761 4186 5487 6866 7040 65 Size: 1183 1513 1425 1301 1379 174 1396 Fragments arranged by size: 1513 1425 1396 1379 1301 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2086 3930 5522 5571 7015 7202 1700 Size: 110 276 1844 1592 49 1444 187 2869 Fragments arranged by size: 2869 1844 1592 1444 276 187 110 49 BsrGI T'GTAC_A Cuts at: 96 2178 3499 3903 96 Size: 2082 1321 404 4564 Fragments arranged by size: 4564 2082 1321 404 BssHII G'CGCG_C Cuts at: 1925 5589 1925 Size: 3664 4707 BssSI C'ACGA_G Cuts at: 5446 6431 7443 5446 Size: 985 1012 6374 Fragments arranged by size: 6374 1012 985 BstAPI GCAn_nnn'nTGC Cuts at: 794 4117 794 Size: 3323 5048 BstBI TT'CG_AA Cuts at: 7175 7175 Size: 8371 BstEII G'GTnAC_C Cuts at: 1184 2047 3169 3331 4555 1184 Size: 863 1122 162 1224 5000 Fragments arranged by size: 5000 1224 1122 863 162 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2333 3808 4010 2011 Size: 322 1475 202 6372 Fragments arranged by size: 6372 1475 322 202 BstYI r'GATC_y Cuts at: 1972 2481 4386 4422 5363 5480 5701 6068 6453 Size: 509 1905 36 941 117 221 367 385 Cuts at: 6453 6470 7922 8008 8020 8366 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1905 1452 941 509 385 367 346 221 117 86 36 17 12 Bsu36I CC'TnA_GG Cuts at: 3411 3411 Size: 8371 BtgI C'CryG_G Cuts at: 513 1065 2040 2055 2316 3336 4390 4941 513 Size: 552 975 15 261 1020 1054 551 3943 Fragments arranged by size: 3943 1054 1020 975 552 551 261 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5455 487 Size: 683 56 4229 3403 Fragments arranged by size: 4229 3403 683 56 BtsI GCAGTG_nn' Cuts at: 4596 5309 5412 5887 6681 6701 4596 Size: 713 103 475 794 20 6266 Fragments arranged by size: 6266 794 713 475 103 20 ClaI AT'CG_AT Cuts at: 1554 5133 1554 Size: 3579 4792 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8336 DraI TTT'AAA Cuts at: 2527 3370 5675 2527 Size: 843 2305 5223 Fragments arranged by size: 5223 2305 843 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3571 4057 7378 809 Size: 507 2255 486 3321 1802 Fragments arranged by size: 3321 2255 1802 507 486 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2628 4768 5281 Size: 54 1425 50 275 18 798 2140 513 Cuts at: 5281 5520 5596 5818 6707 8336 8 Size: 239 76 222 889 1629 43 Fragments arranged by size: 2140 1629 1425 889 798 513 275 239 222 76 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5818 1537 Size: 275 4006 4090 Fragments arranged by size: 4090 4006 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4995 6301 1353 Size: 3642 1306 3423 Fragments arranged by size: 3642 3423 1306 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2523 3089 5680 6958 7332 7478 Size: 1064 138 934 566 2591 1278 374 146 Cuts at: 7478 387 Size: 1280 Fragments arranged by size: 2591 1280 1278 1064 934 566 374 146 138 EcoICRI GAG'CTC Cuts at: 2323 2323 Size: 8371 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2294 2559 2806 3590 3737 3752 4121 Size: 393 662 265 247 784 147 15 369 Cuts at: 4121 4302 4565 4874 6434 7031 7818 8086 1239 Size: 181 263 309 1560 597 787 268 1524 Fragments arranged by size: 1560 1524 787 784 662 597 393 369 309 268 265 263 247 181 147 15 EcoNI CCTnn'n_nnAGG Cuts at: 2289 2289 Size: 8371 EcoO109I rG'GnC_Cy Cuts at: 2036 2407 2432 4094 4244 2036 Size: 371 25 1662 150 6163 Fragments arranged by size: 6163 1662 371 150 25 EcoRI G'AATT_C Cuts at: 5680 6058 5680 Size: 378 7993 EcoRV GAT'ATC Cuts at: 3246 3246 Size: 8371 FspI TGC'GCA Cuts at: 6877 6877 Size: 8371 HaeII r_GCGC'y Cuts at: 1678 2921 5093 7518 1678 Size: 1243 2172 2425 2531 Fragments arranged by size: 2531 2425 2172 1243 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2269 2301 2979 3011 3334 Size: 32 135 32 697 32 678 32 323 Cuts at: 3334 3366 3442 3474 3728 3760 4275 4307 5425 Size: 32 76 32 254 32 515 32 1118 Cuts at: 5425 5457 7012 7044 7086 7118 1373 Size: 32 1555 32 42 32 2626 Fragments arranged by size: 2626 1555 1118 697 678 515 323 254 135 76 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3625 3874 5565 5715 5961 669 Size: 988 281 1687 249 1691 150 246 3079 Fragments arranged by size: 3079 1691 1687 988 281 249 246 150 HindIII A'AGCT_T Cuts at: 748 4639 5231 748 Size: 3891 592 3888 Fragments arranged by size: 3891 3888 592 HpaI GTT'AAC Cuts at: 5961 5961 Size: 8371 KasI G'GCGC_C Cuts at: 2917 2917 Size: 8371 KpnI G_GTAC'C Cuts at: 4876 5696 4876 Size: 820 7551 MfeI C'AATT_G Cuts at: 5970 5970 Size: 8371 MluI A'CGCG_T Cuts at: 8247 8247 Size: 8371 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2118 2591 3221 4416 4740 7485 7669 1305 Size: 813 473 630 1195 324 2745 184 2007 Fragments arranged by size: 2745 2007 1195 813 630 473 324 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4770 5283 10 Size: 54 1425 343 2938 513 3098 Fragments arranged by size: 3098 2938 1425 513 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2111 2331 2597 5405 6329 6688 Size: 648 843 102 220 266 2808 924 359 Cuts at: 6688 6847 518 Size: 159 2042 Fragments arranged by size: 2808 2042 924 843 648 359 266 220 159 102 MspA1I CmG'CkG Cuts at: 1606 2042 2057 3202 4135 4483 5787 6464 7612 Size: 436 15 1145 933 348 1304 677 1148 Cuts at: 7612 7857 1606 Size: 245 2120 Fragments arranged by size: 2120 1304 1148 1145 933 677 436 348 245 15 NaeI GCC'GGC Cuts at: 2088 5573 2088 Size: 3485 4886 NarI GG'CG_CC Cuts at: 2918 2918 Size: 8371 NcoI C'CATG_G Cuts at: 513 1065 4941 513 Size: 552 3876 3943 Fragments arranged by size: 3943 3876 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8371 NgoMIV G'CCGG_C Cuts at: 2086 5571 2086 Size: 3485 4886 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8371 NotI GC'GGCC_GC Cuts at: 5818 5818 Size: 8371 NsiI A_TGCA'T Cuts at: 3687 3687 Size: 8371 NspI r_CATG'y Cuts at: 1132 1229 3778 5729 7274 1132 Size: 97 2549 1951 1545 2229 Fragments arranged by size: 2549 2229 1951 1545 97 PasI CC'CwG_GG Cuts at: 1348 2428 4370 1348 Size: 1080 1942 5349 Fragments arranged by size: 5349 1942 1080 PciI A'CATG_T Cuts at: 7270 7270 Size: 8371 PflMI CCAn_nnn'nTGG Cuts at: 1108 2771 2983 1108 Size: 1663 212 6496 Fragments arranged by size: 6496 1663 212 PfoI T'CCnGG_A Cuts at: 3261 3261 Size: 8371 PmeI GTTT'AAAC Cuts at: 5675 5675 Size: 8371 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2957 2989 3018 3050 3929 3961 4994 5026 6440 Size: 32 29 32 879 32 1033 32 1414 Cuts at: 6440 6472 7953 7985 2957 Size: 32 1481 32 3343 Fragments arranged by size: 3343 1481 1414 1033 879 32 32 32 32 32 32 29 PpuMI rG'GwC_Cy Cuts at: 2407 2432 2407 Size: 25 8346 PshAI GACnn'nnGTC Cuts at: 1613 2793 1613 Size: 1180 7191 PsiI TTA'TAA Cuts at: 3855 5941 3855 Size: 2086 6285 PspOMI G'GGCC_C Cuts at: 2036 2036 Size: 8371 PstI C_TGCA'G Cuts at: 830 3751 4591 4970 5562 5723 830 Size: 2921 840 379 592 161 3478 Fragments arranged by size: 3478 2921 840 592 379 161 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8371 PvuII CAG'CTG Cuts at: 3202 4135 3202 Size: 933 7438 SacI G_AGCT'C Cuts at: 2325 2325 Size: 8371 SacII CC_GC'GG Cuts at: 2043 2058 2043 Size: 15 8356 SalI G'TCGA_C Cuts at: 1936 5713 1936 Size: 3777 4594 SbfI CC_TGCA'GG Cuts at: 5723 5723 Size: 8371 ScaI AGT'ACT Cuts at: 6619 8331 6619 Size: 1712 6659 SfcI C'TryA_G Cuts at: 826 1045 3569 3747 4516 4587 4966 5558 5576 Size: 219 2524 178 769 71 379 592 18 Cuts at: 5576 5719 6854 7535 7726 826 Size: 143 1135 681 191 1471 Fragments arranged by size: 2524 1471 1135 769 681 592 379 219 191 178 143 71 18 SfoI GGC'GCC Cuts at: 2919 2919 Size: 8371 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8371 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8371 SmaI CCC'GGG Cuts at: 1872 4677 5698 1872 Size: 2805 1021 4545 Fragments arranged by size: 4545 2805 1021 SmlI C'TyrA_G Cuts at: 820 1017 1943 3104 3309 5057 5388 6475 7376 Size: 197 926 1161 205 1748 331 1087 901 Cuts at: 7376 7638 820 Size: 262 1553 Fragments arranged by size: 1748 1553 1161 1087 926 901 331 262 205 197 SnaBI TAC'GTA Cuts at: 493 6162 493 Size: 5669 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8371 SphI G_CATG'C Cuts at: 1132 5729 1132 Size: 4597 3774 SspI AAT'ATT Cuts at: 5 52 6295 5 Size: 47 6243 2081 Fragments arranged by size: 6243 2081 47 StuI AGG'CCT Cuts at: 1517 4404 4619 1517 Size: 2887 215 5269 Fragments arranged by size: 5269 2887 215 StyI C'CwwG_G Cuts at: 513 1065 2290 4009 4083 4941 5278 5810 513 Size: 552 1225 1719 74 858 337 532 3074 Fragments arranged by size: 3074 1719 1225 858 552 532 337 74 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2486 3485 6398 6415 6568 6753 7198 Size: 1255 906 999 2913 17 153 185 445 Cuts at: 7198 325 Size: 1498 Fragments arranged by size: 2913 1498 1255 999 906 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2178 3499 3903 Size: 274 80 33 51 1443 201 1321 404 Cuts at: 3903 4548 6617 8329 96 Size: 645 2069 1712 138 Fragments arranged by size: 2069 1712 1443 1321 645 404 274 201 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2433 2465 2863 2895 8331 8363 2433 Size: 32 398 32 5436 32 2441 Fragments arranged by size: 5436 2441 398 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3269 1316 Size: 1953 6418 XbaI T'CTAG_A Cuts at: 5707 5707 Size: 8371 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4007 4948 4007 Size: 941 7430 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8371 XmaI C'CCGG_G Cuts at: 1870 4675 5696 1870 Size: 2805 1021 4545 Fragments arranged by size: 4545 2805 1021 XmnI GAAnn'nnTTC Cuts at: 4799 5211 6500 4799 Size: 412 1289 6670 Fragments arranged by size: 6670 1289 412 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6179 276 Size: 53 83 186 936 4645 2468 Fragments arranged by size: 4645 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BsiWI BsmBI BstZ17I DraIII FalI FseI FspAI NruI PacI PmlI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI