Restriction map for
pFN21AB1226
(Linear) (Six-base) MAPSORT of: KIBB1226.seq Check: 5234 from: 1 to: 3666
KIBB1226.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1383 3666
Size: 1383 2283
Acc65I G'GTAC_C
Cuts at: 0 2867 3666
Size: 2867 799
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 2116 2869 3666
Size: 289 1827 753 797
Fragments arranged by size:
1827 797 753 289
AflIII A'CryG_T
Cuts at: 0 1736 1955 3666
Size: 1736 219 1711
Fragments arranged by size:
1736 1711 219
AgeI A'CCGG_T
Cuts at: 0 1925 3666
Size: 1925 1741
AleI CACnn'nnGTG
Cuts at: 0 592 3400 3666
Size: 592 2808 266
Fragments arranged by size:
2808 592 266
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 610 3666
Size: 610 3056
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1013 1045 3666
Size: 1013 32 2621
Fragments arranged by size:
2621 1013 32
AlwNI CAG_nnn'CTG
Cuts at: 0 423 766 1229 1516 1572 2112 2130 2847
Size: 423 343 463 287 56 540 18 717
Cuts at: 2847 3285 3604 3666
Size: 438 319 62
Fragments arranged by size:
717 540 463 438 423 343 319 287
62 56 18
ApaI G_GGCC'C
Cuts at: 0 35 3666
Size: 35 3631
ApoI r'AATT_y
Cuts at: 0 1807 3666
Size: 1807 1859
AseI AT'TA_AT
Cuts at: 0 3161 3666
Size: 3161 505
AvaI C'yCGr_G
Cuts at: 0 2425 2670 3666
Size: 2425 245 996
Fragments arranged by size:
2425 996 245
AvrII C'CTAG_G
Cuts at: 0 285 3666
Size: 285 3381
BamHI G'GATC_C
Cuts at: 0 3475 3666
Size: 3475 191
BanI G'GyrC_C
Cuts at: 0 912 2506 2867 3666
Size: 912 1594 361 799
Fragments arranged by size:
1594 912 799 361
BanII G_rGCy'C
Cuts at: 0 35 320 686 965 2761 2921 3666
Size: 35 285 366 279 1796 160 745
Fragments arranged by size:
1796 745 366 285 279 160 35
BbeI G_GCGC'C
Cuts at: 0 916 3666
Size: 916 2750
BbsI GAAGACnn'nnnn_
Cuts at: 0 366 1952 2048 2894 2984 3658 3666
Size: 366 1586 96 846 90 674 8
Fragments arranged by size:
1586 846 674 366 96 90 8
BbvCI CC'TCA_GC
Cuts at: 0 406 1193 2651 3666
Size: 406 787 1458 1015
Fragments arranged by size:
1458 1015 787 406
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3432 3466 3666
Size: 3432 34 200
Fragments arranged by size:
3432 200 34
BclI T'GATC_A
Cuts at: 0 2480 3188 3666
Size: 2480 708 478
Fragments arranged by size:
2480 708 478
BfrBI ATG'CAT
Cuts at: 0 1680 3666
Size: 1680 1986
BglI GCCn_nnn'nGGC
Cuts at: 0 622 3666
Size: 622 3044
BlpI GC'TnA_GC
Cuts at: 0 67 3666
Size: 67 3599
Bme1580I G_kGCm'C
Cuts at: 0 35 216 3666
Size: 35 181 3450
Fragments arranged by size:
3450 181 35
BmgBI CAC'GTC
Cuts at: 0 138 975 1737 1958 3666
Size: 138 837 762 221 1708
Fragments arranged by size:
1708 837 762 221 138
BmrI ACTGGGnnnn_n'
Cuts at: 0 389 470 2274 3357 3666
Size: 389 81 1804 1083 309
Fragments arranged by size:
1804 1083 389 309 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2829 3666
Size: 2829 837
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 801 1585 1732 1747 2297 2560 3666
Size: 554 247 784 147 15 550 263 1106
Fragments arranged by size:
1106 784 554 550 263 247 147 15
Bpu10I CC'TnA_GC
Cuts at: 0 406 1193 1573 2651 2755 3666
Size: 406 787 380 1078 104 911
Fragments arranged by size:
1078 911 787 406 380 104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1120 1289 3037 3368 3666
Size: 1120 169 1748 331 298
Fragments arranged by size:
1748 1120 331 298 169
BsaI GGTCTCn'nnnn_
Cuts at: 0 819 3666
Size: 819 2847
BsaAI yAC'GTr
Cuts at: 0 3016 3666
Size: 3016 650
BsaBI GATnn'nnATC
Cuts at: 0 1243 2380 2449 2857 3666
Size: 1243 1137 69 408 809
Fragments arranged by size:
1243 1137 809 408 69
BsaHI Gr'CG_yC
Cuts at: 0 913 3666
Size: 913 2753
BsaWI w'CCGG_w
Cuts at: 0 1925 2798 3666
Size: 1925 873 868
Fragments arranged by size:
1925 873 868
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 428 458 799 829 1013 1043 2785 2815
Size: 428 30 341 30 184 30 1742 30
Cuts at: 2815 3666
Size: 851
Fragments arranged by size:
1742 851 428 341 184 30 30 30
30
BseYI C'CCAG_C
Cuts at: 0 27 3666
Size: 27 3639
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 1411 2125 2233 2512 2851 3666
Size: 330 1081 714 108 279 339 815
Fragments arranged by size:
1081 815 714 339 330 279 108
BsiEI CG_ry'CG
Cuts at: 0 311 3666
Size: 311 3355
BsiHKAI G_wGCw'C
Cuts at: 0 320 1148 1652 3666
Size: 320 828 504 2014
Fragments arranged by size:
2014 828 504 320
BsmI GAATG_Cn'
Cuts at: 0 2735 3666
Size: 2735 931
Bsp1286I G_dGCh'C
Cuts at: 0 35 216 320 686 965 1148 1652 2761
Size: 35 181 104 366 279 183 504 1109
Cuts at: 2761 2921 3666
Size: 160 745
Fragments arranged by size:
1109 745 504 366 279 183 181 160
104 35
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1383 3666
Size: 1383 2283
BsrBI CCG'CTC
Cuts at: 0 71 3081 3666
Size: 71 3010 585
Fragments arranged by size:
3010 585 71
BsrDI GCAATG_nn'
Cuts at: 0 756 2181 3482 3666
Size: 756 1425 1301 184
Fragments arranged by size:
1425 1301 756 184
BsrFI r'CCGG_y
Cuts at: 0 81 1925 3517 3566 3666
Size: 81 1844 1592 49 100
Fragments arranged by size:
1844 1592 100 81 49
BsrGI T'GTAC_A
Cuts at: 0 173 1494 1898 3666
Size: 173 1321 404 1768
Fragments arranged by size:
1768 1321 404 173
BssHII G'CGCG_C
Cuts at: 0 3584 3666
Size: 3584 82
BssSI C'ACGA_G
Cuts at: 0 3441 3666
Size: 3441 225
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2112 3666
Size: 2112 1554
BstEII G'GTnAC_C
Cuts at: 0 42 1164 1326 2550 3666
Size: 42 1122 162 1224 1116
Fragments arranged by size:
1224 1122 1116 162 42
BstXI CCAn_nnnn'nTGG
Cuts at: 0 328 1803 2005 3666
Size: 328 1475 202 1661
Fragments arranged by size:
1661 1475 328 202
BstYI r'GATC_y
Cuts at: 0 476 2381 2417 3358 3475 3666
Size: 476 1905 36 941 117 191
Fragments arranged by size:
1905 941 476 191 117 36
Bsu36I CC'TnA_GG
Cuts at: 0 1406 3666
Size: 1406 2260
BtgI C'CryG_G
Cuts at: 0 35 50 311 1331 2385 2936 3666
Size: 35 15 261 1020 1054 551 730
Fragments arranged by size:
1054 1020 730 551 261 35 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3450 3666
Size: 3450 216
BtsI GCAGTG_nn'
Cuts at: 0 2591 3304 3407 3666
Size: 2591 713 103 259
Fragments arranged by size:
2591 713 259 103
ClaI AT'CG_AT
Cuts at: 0 3128 3666
Size: 3128 538
DraI TTT'AAA
Cuts at: 0 522 1365 3666
Size: 522 843 2301
Fragments arranged by size:
2301 843 522
DrdI GACnn_nn'nnGTC
Cuts at: 0 1566 2052 3666
Size: 1566 486 1614
Fragments arranged by size:
1614 1566 486
EaeI y'GGCC_r
Cuts at: 0 623 2763 3276 3515 3591 3666
Size: 623 2140 513 239 76 75
Fragments arranged by size:
2140 623 513 239 76 75
EarI CTCTTCn'nnn_
Cuts at: 0 2990 3666
Size: 2990 676
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 518 1084 3666
Size: 518 566 2582
Fragments arranged by size:
2582 566 518
EcoICRI GAG'CTC
Cuts at: 0 318 3666
Size: 318 3348
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 554 801 1585 1732 1747 2116 2297
Size: 289 265 247 784 147 15 369 181
Cuts at: 2297 2560 2869 3666
Size: 263 309 797
Fragments arranged by size:
797 784 369 309 289 265 263 247
181 147 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 284 3666
Size: 284 3382
EcoO109I rG'GnC_Cy
Cuts at: 0 31 402 427 2089 2239 3666
Size: 31 371 25 1662 150 1427
Fragments arranged by size:
1662 1427 371 150 31 25
EcoRV GAT'ATC
Cuts at: 0 1241 3666
Size: 1241 2425
HaeII r_GCGC'y
Cuts at: 0 916 3088 3666
Size: 916 2172 578
Fragments arranged by size:
2172 916 578
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 264 296 974 1006 1329 1361 1437 1469
Size: 264 32 678 32 323 32 76 32
Cuts at: 1469 1723 1755 2270 2302 3420 3452 3666
Size: 254 32 515 32 1118 32 214
Fragments arranged by size:
1118 678 515 323 264 254 214 76
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1620 1869 3560 3666
Size: 1620 249 1691 106
Fragments arranged by size:
1691 1620 249 106
HindIII A'AGCT_T
Cuts at: 0 2634 3226 3666
Size: 2634 592 440
Fragments arranged by size:
2634 592 440
KasI G'GCGC_C
Cuts at: 0 912 3666
Size: 912 2754
KpnI G_GTAC'C
Cuts at: 0 2871 3666
Size: 2871 795
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 586 1216 2411 2735 3666
Size: 113 473 630 1195 324 931
Fragments arranged by size:
1195 931 630 473 324 113
MscI TGG'CCA
Cuts at: 0 2765 3278 3666
Size: 2765 513 388
Fragments arranged by size:
2765 513 388
MslI CAynn'nnrTG
Cuts at: 0 106 326 592 3400 3666
Size: 106 220 266 2808 266
Fragments arranged by size:
2808 266 266 220 106
MspA1I CmG'CkG
Cuts at: 0 37 52 1197 2130 2478 3666
Size: 37 15 1145 933 348 1188
Fragments arranged by size:
1188 1145 933 348 37 15
NaeI GCC'GGC
Cuts at: 0 83 3568 3666
Size: 83 3485 98
Fragments arranged by size:
3485 98 83
NarI GG'CG_CC
Cuts at: 0 913 3666
Size: 913 2753
NcoI C'CATG_G
Cuts at: 0 2936 3666
Size: 2936 730
NgoMIV G'CCGG_C
Cuts at: 0 81 3566 3666
Size: 81 3485 100
Fragments arranged by size:
3485 100 81
NsiI A_TGCA'T
Cuts at: 0 1682 3666
Size: 1682 1984
NspI r_CATG'y
Cuts at: 0 1773 3666
Size: 1773 1893
PasI CC'CwG_GG
Cuts at: 0 423 2365 3666
Size: 423 1942 1301
Fragments arranged by size:
1942 1301 423
PflMI CCAn_nnn'nTGG
Cuts at: 0 766 978 3666
Size: 766 212 2688
Fragments arranged by size:
2688 766 212
PfoI T'CCnGG_A
Cuts at: 0 1256 3666
Size: 1256 2410
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 952 984 1013 1045 1924 1956 2989 3021
Size: 952 32 29 32 879 32 1033 32
Cuts at: 3021 3666
Size: 645
Fragments arranged by size:
1033 952 879 645 32 32 32 32
29
PpuMI rG'GwC_Cy
Cuts at: 0 402 427 3666
Size: 402 25 3239
Fragments arranged by size:
3239 402 25
PshAI GACnn'nnGTC
Cuts at: 0 788 3666
Size: 788 2878
PsiI TTA'TAA
Cuts at: 0 1850 3666
Size: 1850 1816
PspOMI G'GGCC_C
Cuts at: 0 31 3666
Size: 31 3635
PstI C_TGCA'G
Cuts at: 0 1746 2586 2965 3557 3666
Size: 1746 840 379 592 109
Fragments arranged by size:
1746 840 592 379 109
PvuII CAG'CTG
Cuts at: 0 1197 2130 3666
Size: 1197 933 1536
Fragments arranged by size:
1536 1197 933
SacI G_AGCT'C
Cuts at: 0 320 3666
Size: 320 3346
SacII CC_GC'GG
Cuts at: 0 38 53 3666
Size: 38 15 3613
Fragments arranged by size:
3613 38 15
SfcI C'TryA_G
Cuts at: 0 1564 1742 2511 2582 2961 3553 3571 3666
Size: 1564 178 769 71 379 592 18 95
Fragments arranged by size:
1564 769 592 379 178 95 71 18
SfoI GGC'GCC
Cuts at: 0 914 3666
Size: 914 2752
SmaI CCC'GGG
Cuts at: 0 2672 3666
Size: 2672 994
SmlI C'TyrA_G
Cuts at: 0 1099 1304 3052 3383 3666
Size: 1099 205 1748 331 283
Fragments arranged by size:
1748 1099 331 283 205
StuI AGG'CCT
Cuts at: 0 2399 2614 3666
Size: 2399 215 1052
Fragments arranged by size:
2399 1052 215
StyI C'CwwG_G
Cuts at: 0 285 2004 2078 2936 3273 3666
Size: 285 1719 74 858 337 393
Fragments arranged by size:
1719 858 393 337 285 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 481 1480 3666
Size: 481 999 2186
Fragments arranged by size:
2186 999 481
TatI w'GTAC_w
Cuts at: 0 173 1494 1898 2543 3666
Size: 173 1321 404 645 1123
Fragments arranged by size:
1321 1123 645 404 173
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 428 460 858 890 3666
Size: 428 32 398 32 2776
Fragments arranged by size:
2776 428 398 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1264 3666
Size: 1264 2402
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2002 2943 3666
Size: 2002 941 723
Fragments arranged by size:
2002 941 723
XmaI C'CCGG_G
Cuts at: 0 2670 3666
Size: 2670 996
XmnI GAAnn'nnTTC
Cuts at: 0 2794 3206 3666
Size: 2794 412 460
Fragments arranged by size:
2794 460 412
Enzymes that do cut:
AarI Acc65I AcuI AflIII AgeI AleI AlfI AloI
AlwNI ApaI ApoI AseI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BclI BfrBI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I
HincII HindIII KasI KpnI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PflMI
PfoI PpiI PpuMI PshAI PsiI PspOMI PstI PvuII
SacI SacII SfcI SfoI SmaI SmlI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AhdI ApaLI AscI
AsiSI BaeI BciVI BglII BmtI BseRI BsiWI BsmBI
BspEI BspHI BstBI BstZ17I CspCI DraIII EagI EcoRI
FalI FseI FspI FspAI HpaI MfeI MluI NdeI
NheI NotI NruI PacI PciI PmeI PmlI PspXI
PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI XbaI XhoI ZraI