(Circular) (Six-base) MAPSORT of: pFN21AB0497.seq Check: 5019 from: 1 to: 17986 pFN21AB0497 17986 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4664 7395 8596 10591 13034 13735 4664 Size: 2731 1201 1995 2443 701 8915 Fragments arranged by size: 8915 2731 2443 1995 1201 701 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 15796 278 Size: 53 83 186 936 14260 2468 Fragments arranged by size: 14260 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2421 4198 4317 6236 8679 9183 9411 10815 Size: 484 1777 119 1919 2443 504 228 1404 Cuts at: 10815 13014 13298 14666 15329 1937 Size: 2199 284 1368 663 4594 Fragments arranged by size: 4594 2443 2199 1919 1777 1404 1368 663 504 484 284 228 119 Acc65I G'GTAC_C Cuts at: 15307 15307 Size: 17986 AclI AA'CG_TT Cuts at: 1563 14907 16113 16486 1563 Size: 13344 1206 373 3063 Fragments arranged by size: 13344 3063 1206 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2396 3050 5186 5267 5438 5648 6057 8037 Size: 764 654 2136 81 171 210 409 1980 Cuts at: 8037 9710 10115 10160 10684 11531 11832 11846 12020 Size: 1673 405 45 524 847 301 14 174 Cuts at: 12020 12266 12308 14874 16049 17433 17701 1632 Size: 246 42 2566 1175 1384 268 1917 Fragments arranged by size: 2566 2136 1980 1917 1673 1384 1175 847 764 654 524 409 405 301 268 246 210 174 171 81 45 42 14 AfeI AGC'GCT Cuts at: 9809 13592 9809 Size: 3783 14203 AflII C'TTAA_G Cuts at: 820 1017 14111 820 Size: 197 13094 4695 Fragments arranged by size: 13094 4695 197 AflIII A'CryG_T Cuts at: 1203 4884 5541 9336 9477 9786 10645 13877 14146 Size: 3681 657 3795 141 309 859 3232 269 Cuts at: 14146 16885 17862 1203 Size: 2739 977 1327 Fragments arranged by size: 3795 3681 3232 2739 1327 977 859 657 309 269 141 AgeI A'CCGG_T Cuts at: 14232 16817 14232 Size: 2585 15401 AhdI GACnn_n'nnGTC Cuts at: 3816 7510 9620 15217 16715 3816 Size: 3694 2110 5597 1498 5087 Fragments arranged by size: 5597 5087 3694 2110 1498 AleI CACnn'nnGTG Cuts at: 1166 12758 14876 1166 Size: 11592 2118 4276 Fragments arranged by size: 11592 4276 2118 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2130 6416 8612 11618 12587 13376 14367 15230 Size: 946 4286 2196 3006 969 789 991 863 Cuts at: 15230 1184 Size: 3940 Fragments arranged by size: 4286 3940 3006 2196 991 969 946 863 789 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2825 2857 8525 8557 2825 Size: 32 5668 32 12254 Fragments arranged by size: 12254 5668 32 32 AlwNI CAG_nnn'CTG Cuts at: 3424 5206 5671 6211 6441 6665 7621 7903 9301 Size: 1782 465 540 230 224 956 282 1398 Cuts at: 9301 10315 10707 11002 11242 11902 12208 12244 12544 Size: 1014 392 295 240 660 306 36 300 Cuts at: 12544 12559 15729 17301 3424 Size: 15 3170 1572 4109 Fragments arranged by size: 4109 3170 1782 1572 1398 1014 956 660 540 465 392 306 300 295 282 240 230 224 36 15 ApaI G_GGCC'C Cuts at: 2501 6735 9602 11308 14818 2501 Size: 4234 2867 1706 3510 5669 Fragments arranged by size: 5669 4234 3510 2867 1706 ApaLI G'TGCA_C Cuts at: 3656 3746 16043 17199 3656 Size: 90 12297 1156 4443 Fragments arranged by size: 12297 4443 1156 90 ApoI r'AATT_y Cuts at: 1493 2815 4331 4646 5435 5507 7152 10553 15295 Size: 1322 1516 315 789 72 1645 3401 4742 Cuts at: 15295 15521 15673 17782 1493 Size: 226 152 2109 1697 Fragments arranged by size: 4742 3401 2109 1697 1645 1516 1322 789 315 226 152 72 AseI AT'TA_AT Cuts at: 160 1032 16540 160 Size: 872 15508 1606 Fragments arranged by size: 15508 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 17986 AvaI C'yCGr_G Cuts at: 1870 1943 2082 2219 2250 3144 4132 6766 7161 Size: 73 139 137 31 894 988 2634 395 Cuts at: 7161 7321 8600 8840 10266 10590 10784 12162 14451 Size: 160 1279 240 1426 324 194 1378 2289 Cuts at: 14451 15311 1870 Size: 860 4545 Fragments arranged by size: 4545 2634 2289 1426 1378 1279 988 894 860 395 324 240 194 160 139 137 73 31 AvrII C'CTAG_G Cuts at: 6376 8383 6376 Size: 2007 15979 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 8836 8869 14099 14132 8836 Size: 33 5230 33 12690 Fragments arranged by size: 12690 5230 33 33 BamHI G'GATC_C Cuts at: 8493 9936 10693 10710 13753 15316 15683 8493 Size: 1443 757 17 3043 1563 367 10796 Fragments arranged by size: 10796 3043 1563 1443 757 367 17 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2950 3245 5139 5323 7792 Size: 325 217 627 1163 295 1894 184 2469 Cuts at: 7792 8830 9172 9704 10528 10603 13287 14169 15307 Size: 1038 342 532 824 75 2684 882 1138 Cuts at: 15307 16762 618 Size: 1455 1842 Fragments arranged by size: 2684 2469 1894 1842 1455 1163 1138 1038 882 824 627 532 342 325 295 217 184 75 BanII G_rGCy'C Cuts at: 1391 2154 2220 2501 3621 4270 5685 6735 7403 Size: 763 66 281 1120 649 1415 1050 668 Cuts at: 7403 8760 9044 9602 10104 10440 11308 11418 11649 Size: 1357 284 558 502 336 868 110 231 Cuts at: 11649 14818 15063 15305 1391 Size: 3169 245 242 4072 Fragments arranged by size: 4072 3169 1415 1357 1120 1050 868 763 668 649 558 502 336 284 281 245 242 231 110 66 BbeI G_GCGC'C Cuts at: 2954 5143 14173 2954 Size: 2189 9030 6767 Fragments arranged by size: 9030 6767 2189 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3375 4423 4714 5374 5753 6390 9275 Size: 971 1476 1048 291 660 379 637 2885 Cuts at: 9275 9949 10122 10148 14880 928 Size: 674 173 26 4732 4034 Fragments arranged by size: 4732 4034 2885 1476 1048 971 674 660 637 379 291 173 26 BbvCI CC'TCA_GC Cuts at: 3433 8411 8748 10514 10679 11709 12499 3433 Size: 4978 337 1766 165 1030 790 8920 Fragments arranged by size: 8920 4978 1766 1030 790 337 165 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5282 5316 7570 7604 8141 8175 13259 Size: 34 3345 34 2254 34 537 34 5084 Cuts at: 13259 13293 16177 16211 1903 Size: 34 2884 34 3678 Fragments arranged by size: 5084 3678 3345 2884 2254 537 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3347 3841 5531 8671 12302 14982 15878 17088 3347 Size: 494 1690 3140 3631 2680 896 1210 4245 Fragments arranged by size: 4245 3631 3140 2680 1690 1210 896 494 BclI T'GATC_A Cuts at: 1548 3096 3426 5986 6957 9438 9711 11472 15238 Size: 1548 330 2560 971 2481 273 1761 3766 Cuts at: 15238 1548 Size: 4296 Fragments arranged by size: 4296 3766 2560 2481 1761 1548 971 330 273 BfrBI ATG'CAT Cuts at: 2859 9835 2859 Size: 6976 11010 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2084 7419 8651 Size: 107 122 71 631 605 412 5335 1232 Cuts at: 8651 10325 11303 12842 14813 16597 136 Size: 1674 978 1539 1971 1784 1525 Fragments arranged by size: 5335 1971 1784 1674 1539 1525 1232 978 631 605 412 122 107 71 BglII A'GATC_T Cuts at: 2550 4896 6063 8089 10857 11263 12024 17981 2550 Size: 2346 1167 2026 2768 406 761 5957 2555 Fragments arranged by size: 5957 2768 2555 2346 2026 1167 761 406 BlpI GC'TnA_GC Cuts at: 2148 3990 7223 11679 13781 14066 15403 2148 Size: 1842 3233 4456 2102 285 1337 4731 Fragments arranged by size: 4731 4456 3233 2102 1842 1337 285 Bme1580I G_kGCm'C Cuts at: 1790 2501 2658 2791 3250 3660 3750 6735 7942 Size: 711 157 133 459 410 90 2985 1207 Cuts at: 7942 8835 9177 9602 10197 10533 11308 12181 13555 Size: 893 342 425 595 336 775 873 1374 Cuts at: 13555 14818 15260 16047 17203 1790 Size: 1263 442 787 1156 2573 Fragments arranged by size: 2985 2573 1374 1263 1207 1156 893 873 787 775 711 595 459 442 425 410 342 336 157 133 90 BmgBI CAC'GTC Cuts at: 1318 3457 10449 13880 1318 Size: 2139 6992 3431 5424 Fragments arranged by size: 6992 5424 3431 2139 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3625 3952 4162 Size: 416 53 83 399 24 2208 327 210 Cuts at: 4162 5225 5731 5860 5959 6298 7252 9273 13216 Size: 1063 506 129 99 339 954 2021 3943 Cuts at: 13216 13681 14170 15717 16675 442 Size: 465 489 1547 958 1753 Fragments arranged by size: 3943 2208 2021 1753 1547 1063 958 954 506 489 465 416 399 339 327 210 129 99 83 53 24 BmtI G_CTAG'C Cuts at: 1056 14540 1056 Size: 13484 4502 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2669 2849 3299 3635 5168 5228 5366 5678 Size: 1430 180 450 336 1533 60 138 312 Cuts at: 5678 5693 6653 7569 8750 9292 9521 11108 11330 Size: 15 960 916 1181 542 229 1587 222 Cuts at: 11330 11768 11852 11924 12566 12614 12677 12794 12989 Size: 438 84 72 642 48 63 117 195 Cuts at: 12989 13508 14267 14460 16646 1239 Size: 519 759 193 2186 2579 Fragments arranged by size: 2579 2186 1587 1533 1430 1181 960 916 759 642 542 519 450 438 336 312 229 222 195 193 180 138 117 84 72 63 60 48 15 Bpu10I CC'TnA_GC Cuts at: 2169 3090 3433 5120 8411 8748 9471 10514 10519 Size: 921 343 1687 3291 337 723 1043 5 Cuts at: 10519 10679 11709 12499 12588 2169 Size: 160 1030 790 89 7567 Fragments arranged by size: 7567 3291 1687 1043 1030 921 790 723 343 337 160 89 5 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2175 4124 4856 5048 7025 10424 12632 14489 16111 Size: 1949 732 192 1977 3399 2208 1857 1622 Cuts at: 16111 16976 17274 2175 Size: 865 298 2887 Fragments arranged by size: 3399 2887 2208 1977 1949 1857 1622 865 732 298 192 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2735 5780 6572 7430 8135 8322 12488 Size: 617 1236 3045 792 858 705 187 4166 Cuts at: 12488 12770 12779 14657 16649 882 Size: 282 9 1878 1992 2219 Fragments arranged by size: 4166 3045 2219 1992 1878 1236 858 792 705 617 282 187 9 BsaAI yAC'GTr Cuts at: 493 1204 3625 3976 9415 13136 15777 493 Size: 711 2421 351 5439 3721 2641 2702 Fragments arranged by size: 5439 3721 2702 2641 2421 711 351 BsaBI GATnn'nnATC Cuts at: 13373 13758 17980 13373 Size: 385 4222 13379 Fragments arranged by size: 13379 4222 385 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2951 3670 3965 5140 Size: 53 83 186 936 1418 719 295 1175 Cuts at: 5140 10306 12129 14170 15793 16175 16810 275 Size: 5166 1823 2041 1623 382 635 1451 Fragments arranged by size: 5166 2041 1823 1623 1451 1418 1175 936 719 635 382 295 186 83 53 BsaWI w'CCGG_w Cuts at: 3638 6486 6879 14232 15030 16419 16817 17091 17238 Size: 2848 393 7353 798 1389 398 274 147 Cuts at: 17238 17649 3638 Size: 411 3975 Fragments arranged by size: 7353 3975 2848 1389 798 411 398 393 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2825 2855 4901 4931 8525 Size: 30 165 30 1422 30 2046 30 3594 Cuts at: 8525 8555 12119 12149 13427 13457 14909 14939 15176 Size: 30 3564 30 1278 30 1452 30 237 Cuts at: 15176 15206 1178 Size: 30 3958 Fragments arranged by size: 3958 3594 3564 2046 1452 1422 1278 237 165 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2177 2846 5030 5741 9011 11147 11807 11900 Size: 991 669 2184 711 3270 2136 660 93 Cuts at: 11900 11921 12611 13634 14105 1186 Size: 21 690 1023 471 5067 Fragments arranged by size: 5067 3270 2184 2136 1023 991 711 690 669 660 471 93 21 BseYI C'CCAG_C Cuts at: 2386 3204 3651 5135 6734 8911 9489 10061 10427 Size: 818 447 1484 1599 2177 578 572 366 Cuts at: 10427 10723 10981 11362 11535 11840 12552 12559 12837 Size: 296 258 381 173 305 712 7 278 Cuts at: 12837 13587 15024 17189 17685 2386 Size: 750 1437 2165 496 2687 Fragments arranged by size: 2687 2177 2165 1599 1484 1437 818 750 712 578 572 496 447 381 366 305 296 278 258 173 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2498 2591 2909 3293 4133 4235 4373 Size: 573 762 93 318 384 840 102 138 Cuts at: 4373 5027 5099 5138 6362 6689 7373 7916 8242 Size: 654 72 39 1224 327 684 543 326 Cuts at: 8242 8487 9386 10646 10670 10723 11174 12353 12734 Size: 245 899 1260 24 53 451 1179 381 Cuts at: 12734 1163 Size: 6415 Fragments arranged by size: 6415 1260 1224 1179 899 840 762 684 654 573 543 451 384 381 327 326 318 245 138 102 93 72 53 39 24 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 11566 15436 16197 17225 1540 Size: 275 186 9565 3870 761 1028 2301 Fragments arranged by size: 9565 3870 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2154 3660 3709 3750 5685 6494 9327 10212 10440 Size: 1506 49 41 1935 809 2833 885 228 Cuts at: 10440 10674 12867 13575 16047 16132 17203 2154 Size: 234 2193 708 2472 85 1071 2937 Fragments arranged by size: 2937 2833 2472 2193 1935 1506 1071 885 809 708 234 228 85 49 41 BsiWI C'GTAC_G Cuts at: 9545 9545 Size: 17986 BsmI GAATG_Cn' Cuts at: 2119 3834 4011 8289 15497 15590 2119 Size: 1715 177 4278 7208 93 4515 Fragments arranged by size: 7208 4515 4278 1715 177 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3956 11575 3956 Size: 7619 10367 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2154 2220 2501 2658 2791 3250 3621 Size: 399 364 66 281 157 133 459 371 Cuts at: 3621 3660 3709 3750 4270 5685 6494 6735 7403 Size: 39 49 41 520 1415 809 241 668 Cuts at: 7403 7942 8760 8835 9044 9177 9327 9602 10104 Size: 539 818 75 209 133 150 275 502 Cuts at: 10104 10197 10212 10440 10533 10674 11308 11418 11649 Size: 93 15 228 93 141 634 110 231 Cuts at: 11649 12181 12867 13555 13575 14818 15063 15260 15305 Size: 532 686 688 20 1243 245 197 45 Cuts at: 15305 16047 16132 17203 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1415 1243 1071 818 809 742 688 686 668 634 539 532 520 502 459 399 371 364 281 275 245 241 231 228 209 197 157 150 141 133 133 110 93 93 85 75 66 49 45 41 39 20 15 BspEI T'CCGG_A Cuts at: 3638 6486 6879 17649 3638 Size: 2848 393 10770 3975 Fragments arranged by size: 10770 3975 2848 393 BspHI T'CATG_A Cuts at: 3057 3687 4410 7047 13254 15270 15875 17605 3057 Size: 630 723 2637 6207 2016 605 1730 3438 Fragments arranged by size: 6207 3438 2637 2016 1730 723 630 605 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3701 4014 4395 4664 5928 6636 7395 8596 Size: 2857 313 381 269 1264 708 759 1201 Cuts at: 8596 10591 10668 12267 12285 13034 13735 13813 13936 Size: 1995 77 1599 18 749 701 78 123 Cuts at: 13936 15222 15341 844 Size: 1286 119 3489 Fragments arranged by size: 3489 2857 1995 1599 1286 1264 1201 759 749 708 701 381 313 269 123 119 78 77 18 BsrBI CCG'CTC Cuts at: 1394 1823 3949 7021 12583 14719 15873 1394 Size: 429 2126 3072 5562 2136 1154 3507 Fragments arranged by size: 5562 3507 3072 2136 2126 1154 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3854 4974 5095 6114 7379 7809 8646 Size: 1183 2606 1120 121 1019 1265 430 837 Cuts at: 8646 13360 14121 16481 16655 65 Size: 4714 761 2360 174 1396 Fragments arranged by size: 4714 2606 2360 1396 1265 1183 1120 1019 837 761 430 174 121 BsrFI r'CCGG_y Cuts at: 1700 1810 5754 7326 7646 9966 12692 14126 14166 Size: 110 3944 1572 320 2320 2726 1434 40 Cuts at: 14166 14232 16630 16817 1700 Size: 66 2398 187 2869 Fragments arranged by size: 3944 2869 2726 2398 2320 1572 1434 320 187 110 66 40 BsrGI T'GTAC_A Cuts at: 96 2398 4366 4473 7872 9789 96 Size: 2302 1968 107 3399 1917 8293 Fragments arranged by size: 8293 3399 2302 1968 1917 107 BssHII G'CGCG_C Cuts at: 1925 3647 1925 Size: 1722 16264 BssSI C'ACGA_G Cuts at: 5234 5291 5715 11849 13278 13514 16046 17058 5234 Size: 57 424 6134 1429 236 2532 1012 6162 Fragments arranged by size: 6162 6134 2532 1429 1012 424 236 57 BstAPI GCAn_nnn'nTGC Cuts at: 794 2856 3316 3469 4468 9301 10207 10669 11002 Size: 2062 460 153 999 4833 906 462 333 Cuts at: 11002 13369 794 Size: 2367 5411 Fragments arranged by size: 5411 4833 2367 2062 999 906 462 460 333 153 BstBI TT'CG_AA Cuts at: 3790 5505 8775 13041 16790 3790 Size: 1715 3270 4266 3749 4986 Fragments arranged by size: 4986 4266 3749 3270 1715 BstEII G'GTnAC_C Cuts at: 1184 4402 6859 10366 1184 Size: 3218 2457 3507 8804 Fragments arranged by size: 8804 3507 3218 2457 BstXI CCAn_nnnn'nTGG Cuts at: 5533 5584 6725 7670 8251 9244 10951 13087 13338 Size: 51 1141 945 581 993 1707 2136 251 Cuts at: 13338 14785 5533 Size: 1447 8734 Fragments arranged by size: 8734 2136 1707 1447 1141 993 945 581 251 51 BstYI r'GATC_y Cuts at: 1972 2550 2976 3544 4125 4812 4896 5277 5970 Size: 578 426 568 581 687 84 381 693 Cuts at: 5970 6063 6972 7252 7617 7704 7990 8089 8493 Size: 93 909 280 365 87 286 99 404 Cuts at: 8493 9936 10126 10563 10693 10710 10857 11263 12024 Size: 1443 190 437 130 17 147 406 761 Cuts at: 12024 12666 13753 14496 14580 15316 15683 16068 16085 Size: 642 1087 743 84 736 367 385 17 Cuts at: 16085 17537 17623 17635 17981 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1443 1087 909 761 743 736 693 687 642 581 578 568 437 426 406 404 385 381 367 365 346 286 280 190 147 130 99 93 87 86 84 84 17 17 12 BstZ17I GTA'TAC Cuts at: 4199 4318 13299 4199 Size: 119 8981 8886 Fragments arranged by size: 8981 8886 119 Bsu36I CC'TnA_GG Cuts at: 6899 12195 14328 6899 Size: 5296 2133 10557 Fragments arranged by size: 10557 5296 2133 BtgI C'CryG_G Cuts at: 513 1065 2174 5781 5790 5797 7374 7515 7785 Size: 552 1109 3607 9 7 1577 141 270 Cuts at: 7785 8511 8937 9255 9594 9798 10686 11610 13246 Size: 726 426 318 339 204 888 924 1636 Cuts at: 13246 513 Size: 5253 Fragments arranged by size: 5253 3607 1636 1577 1109 924 888 726 552 426 339 318 270 204 141 9 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4913 6852 8023 8575 8609 9262 Size: 683 56 3687 1939 1171 552 34 653 Cuts at: 9262 12610 13337 13615 487 Size: 3348 727 278 4858 Fragments arranged by size: 4858 3687 3348 1939 1171 727 683 653 552 278 56 34 BtsI GCAGTG_nn' Cuts at: 2286 4469 4532 5083 6960 11132 11276 14871 15258 Size: 2183 63 551 1877 4172 144 3595 387 Cuts at: 15258 15502 16296 16316 2286 Size: 244 794 20 3956 Fragments arranged by size: 4172 3956 3595 2183 1877 794 551 387 244 144 63 20 ClaI AT'CG_AT Cuts at: 1554 12066 1554 Size: 10512 7474 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 7792 7827 14122 14157 529 Size: 35 7228 35 6295 35 4358 Fragments arranged by size: 7228 6295 4358 35 35 35 DraI TTT'AAA Cuts at: 15290 15290 Size: 17986 DraIII CAC_nnn'GTG Cuts at: 2201 3469 4191 6999 8523 10669 13675 13975 2201 Size: 1268 722 2808 1524 2146 3006 300 6212 Fragments arranged by size: 6212 3006 2808 2146 1524 1268 722 300 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3019 10311 12614 16993 809 Size: 507 1703 7292 2303 4379 1802 Fragments arranged by size: 7292 4379 2303 1802 1703 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2041 2094 3605 Size: 54 1425 50 275 18 211 53 1511 Cuts at: 3605 5259 5586 5752 6946 7735 7860 8652 8886 Size: 1654 327 166 1194 789 125 792 234 Cuts at: 8886 9222 11563 14338 14475 14775 15433 16322 17951 Size: 336 2341 2775 137 300 658 889 1629 Cuts at: 17951 8 Size: 43 Fragments arranged by size: 2775 2341 1654 1629 1511 1425 1194 889 792 789 658 336 327 300 275 234 211 166 137 125 54 53 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 11563 15433 1537 Size: 275 9751 3870 4090 Fragments arranged by size: 9751 4090 3870 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2246 3170 4332 4595 4963 5592 7998 8729 Size: 893 924 1162 263 368 629 2406 731 Cuts at: 8729 9002 9735 9848 10217 10554 10631 11265 11286 Size: 273 733 113 369 337 77 634 21 Cuts at: 11286 11808 14451 14673 15116 15916 1353 Size: 522 2643 222 443 800 3423 Fragments arranged by size: 3423 2643 2406 1162 924 893 800 733 731 634 629 522 443 369 368 337 273 263 222 113 77 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2288 7262 7763 9500 10703 13760 Size: 1064 138 699 4974 501 1737 1203 3057 Cuts at: 13760 16573 16947 17093 387 Size: 2813 374 146 1280 Fragments arranged by size: 4974 3057 2813 1737 1280 1203 1064 699 501 374 146 138 EcoICRI GAG'CTC Cuts at: 2152 5683 10438 2152 Size: 3531 4755 9700 Fragments arranged by size: 9700 4755 3531 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2396 2669 2849 3050 3299 3635 5168 Size: 393 764 273 180 201 249 336 1533 Cuts at: 5168 5186 5228 5267 5366 5438 5648 5678 5693 Size: 18 42 39 99 72 210 30 15 Cuts at: 5693 6057 6653 7569 8037 8750 9292 9521 9710 Size: 364 596 916 468 713 542 229 189 Cuts at: 9710 10115 10160 10684 11108 11330 11531 11768 11832 Size: 405 45 524 424 222 201 237 64 Cuts at: 11832 11846 11852 11924 12020 12266 12308 12566 12614 Size: 14 6 72 96 246 42 258 48 Cuts at: 12614 12677 12794 12989 13508 14267 14460 14874 16049 Size: 63 117 195 519 759 193 414 1175 Cuts at: 16049 16646 17433 17701 1239 Size: 597 787 268 1524 Fragments arranged by size: 1533 1524 1175 916 787 764 759 713 597 596 542 524 519 468 424 414 405 393 364 336 273 268 258 249 246 237 229 222 210 201 201 195 193 189 180 117 99 96 72 72 64 63 48 45 42 42 39 30 18 15 14 6 EcoNI CCTnn'n_nnAGG Cuts at: 3490 4391 5675 7454 8198 10784 11291 11906 13391 Size: 901 1284 1779 744 2586 507 615 1485 Cuts at: 13391 13613 13649 14573 3490 Size: 222 36 924 6903 Fragments arranged by size: 6903 2586 1779 1485 1284 924 901 744 615 507 222 36 EcoO109I rG'GnC_Cy Cuts at: 2186 2326 2497 2498 2822 3224 3400 4003 4087 Size: 140 171 1 324 402 176 603 84 Cuts at: 4087 4324 5659 6731 6815 6871 7412 7447 7459 Size: 237 1335 1072 84 56 541 35 12 Cuts at: 7459 7498 8632 9251 9486 9599 10777 11305 11523 Size: 39 1134 619 235 113 1178 528 218 Cuts at: 11523 11899 11911 11969 12205 12241 12541 13624 13858 Size: 376 12 58 236 36 300 1083 234 Cuts at: 13858 14464 15001 2186 Size: 606 537 5171 Fragments arranged by size: 5171 1335 1178 1134 1083 1072 619 606 603 541 537 528 402 376 324 300 237 236 235 234 218 176 171 140 113 84 84 58 56 39 36 35 12 12 1 EcoRI G'AATT_C Cuts at: 4646 5507 15295 15673 4646 Size: 861 9788 378 6959 Fragments arranged by size: 9788 6959 861 378 EcoRV GAT'ATC Cuts at: 10405 11557 10405 Size: 1152 16834 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2257 4936 5272 2257 Size: 2679 336 14971 Fragments arranged by size: 14971 2679 336 FspI TGC'GCA Cuts at: 4010 12410 16492 4010 Size: 8400 4082 5504 Fragments arranged by size: 8400 5504 4082 FspAI rTGC'GCAy Cuts at: 12410 12410 Size: 17986 HaeII r_GCGC'y Cuts at: 1678 2954 4942 5143 7978 9139 9811 13594 14173 Size: 1276 1988 201 2835 1161 672 3783 579 Cuts at: 14173 15129 17133 1678 Size: 956 2004 2531 Fragments arranged by size: 3783 2835 2531 2004 1988 1276 1161 956 672 579 201 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2620 2652 3797 3802 3829 Size: 32 135 32 1048 32 1145 5 27 Cuts at: 3829 3834 5890 5922 5978 6010 6372 6404 6481 Size: 5 2056 32 56 32 362 32 77 Cuts at: 6481 6513 7496 7528 7583 7615 8594 8626 9606 Size: 32 983 32 55 32 979 32 980 Cuts at: 9606 9638 9899 9931 10389 10421 10553 10585 11330 Size: 32 261 32 458 32 132 32 745 Cuts at: 11330 11362 11480 11512 12119 12151 12178 12210 13497 Size: 32 118 32 607 32 27 32 1287 Cuts at: 13497 13529 13702 13734 14059 14091 14348 14380 14909 Size: 32 173 32 325 32 257 32 529 Cuts at: 14909 14941 15176 15203 15208 15223 15235 15255 16627 Size: 32 235 27 5 15 12 20 1372 Cuts at: 16627 16659 16701 16733 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2056 1372 1287 1145 1048 983 980 979 745 607 529 458 362 325 261 257 235 173 135 132 118 77 56 55 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 27 27 27 20 15 12 5 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 3124 3898 6433 7486 8568 9184 Size: 988 281 1186 774 2535 1053 1082 616 Cuts at: 9184 9334 12004 12520 15330 15576 669 Size: 150 2670 516 2810 246 3079 Fragments arranged by size: 3079 2810 2670 2535 1186 1082 1053 988 774 616 516 281 246 150 HindIII A'AGCT_T Cuts at: 748 6001 7178 9722 748 Size: 5253 1177 2544 9012 Fragments arranged by size: 9012 5253 2544 1177 HpaI GTT'AAC Cuts at: 15576 15576 Size: 17986 KasI G'GCGC_C Cuts at: 2950 5139 14169 2950 Size: 2189 9030 6767 Fragments arranged by size: 9030 6767 2189 KpnI G_GTAC'C Cuts at: 15311 15311 Size: 17986 MfeI C'AATT_G Cuts at: 7779 14689 15585 7779 Size: 6910 896 10180 Fragments arranged by size: 10180 6910 896 MluI A'CGCG_T Cuts at: 10645 17862 10645 Size: 7217 10769 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2160 3825 5410 6706 7152 9080 10890 11061 Size: 855 1665 1585 1296 446 1928 1810 171 Cuts at: 11061 12498 14064 17100 17284 1305 Size: 1437 1566 3036 184 2007 Fragments arranged by size: 3036 2007 1928 1810 1665 1585 1566 1437 1296 855 446 184 171 MscI TGG'CCA Cuts at: 10 64 1489 1832 2096 3607 5261 5588 6948 Size: 54 1425 343 264 1511 1654 327 1360 Cuts at: 6948 7862 8888 9224 14340 14777 10 Size: 914 1026 336 5116 437 3219 Fragments arranged by size: 5116 3219 1654 1511 1425 1360 1026 914 437 343 336 327 264 54 MslI CAynn'nnrTG Cuts at: 518 1166 2690 4395 5088 5582 6857 7592 7668 Size: 648 1524 1705 693 494 1275 735 76 Cuts at: 7668 7715 7790 8249 8594 9446 9830 10460 10929 Size: 47 75 459 345 852 384 630 469 Cuts at: 10929 11150 11600 12569 12758 13074 13580 13835 14349 Size: 221 450 969 189 316 506 255 514 Cuts at: 14349 14600 14660 14783 14876 15944 16303 16462 518 Size: 251 60 123 93 1068 359 159 2042 Fragments arranged by size: 2042 1705 1524 1275 1068 969 852 735 693 648 630 514 506 494 469 459 450 384 359 345 316 255 251 221 189 159 123 93 76 75 60 47 MspA1I CmG'CkG Cuts at: 1606 2887 3204 4364 4833 5977 6400 7903 8268 Size: 1281 317 1160 469 1144 423 1503 365 Cuts at: 8268 8667 8850 8915 10302 11948 12556 12718 12772 Size: 399 183 65 1387 1646 608 162 54 Cuts at: 12772 12841 13564 13741 13917 14480 15402 16079 17227 Size: 69 723 177 176 563 922 677 1148 Cuts at: 17227 17472 1606 Size: 245 2120 Fragments arranged by size: 2120 1646 1503 1387 1281 1160 1148 1144 922 723 677 608 563 469 423 399 365 317 245 183 177 176 162 69 65 54 NaeI GCC'GGC Cuts at: 7328 7648 14128 7328 Size: 320 6480 11186 Fragments arranged by size: 11186 6480 320 NarI GG'CG_CC Cuts at: 2951 5140 14170 2951 Size: 2189 9030 6767 Fragments arranged by size: 9030 6767 2189 NcoI C'CATG_G Cuts at: 513 1065 5781 7515 7785 8937 9594 10686 11610 Size: 552 4716 1734 270 1152 657 1092 924 Cuts at: 11610 513 Size: 6889 Fragments arranged by size: 6889 4716 1734 1152 1092 924 657 552 270 NdeI CA'TA_TG Cuts at: 387 13071 387 Size: 12684 5302 NgoMIV G'CCGG_C Cuts at: 7326 7646 14126 7326 Size: 320 6480 11186 Fragments arranged by size: 11186 6480 320 NheI G'CTAG_C Cuts at: 1052 14536 1052 Size: 13484 4502 NotI GC'GGCC_GC Cuts at: 15433 15433 Size: 17986 NruI TCG'CGA Cuts at: 5111 8594 5111 Size: 3483 14503 NsiI A_TGCA'T Cuts at: 2861 9837 2861 Size: 6976 11010 NspI r_CATG'y Cuts at: 1132 1229 2395 3556 3889 4205 4888 5545 6047 Size: 97 1166 1161 333 316 683 657 502 Cuts at: 6047 8466 8773 9340 9481 9790 9883 10279 11599 Size: 2419 307 567 141 309 93 396 1320 Cuts at: 11599 14150 15139 15344 16889 1132 Size: 2551 989 205 1545 2229 Fragments arranged by size: 2551 2419 2229 1545 1320 1166 1161 989 683 657 567 502 396 333 316 309 307 205 141 97 93 PasI CC'CwG_GG Cuts at: 1348 3250 7462 9605 13609 1348 Size: 1902 4212 2143 4004 5725 Fragments arranged by size: 5725 4212 4004 2143 1902 PciI A'CATG_T Cuts at: 4884 5541 9336 9477 9786 14146 16885 4884 Size: 657 3795 141 309 4360 2739 5985 Fragments arranged by size: 5985 4360 3795 2739 657 309 141 PflMI CCAn_nnn'nTGG Cuts at: 1108 2454 3049 3976 4260 4399 6126 6211 8424 Size: 1346 595 927 284 139 1727 85 2213 Cuts at: 8424 8900 9315 10315 10752 11902 12559 14773 1108 Size: 476 415 1000 437 1150 657 2214 4321 Fragments arranged by size: 4321 2214 2213 1727 1346 1150 1000 927 657 595 476 437 415 284 139 85 PfoI T'CCnGG_A Cuts at: 2979 3339 4113 5145 5343 5961 6510 6540 12096 Size: 360 774 1032 198 618 549 30 5556 Cuts at: 12096 2979 Size: 8869 Fragments arranged by size: 8869 5556 1032 774 618 549 360 198 30 PmeI GTTT'AAAC Cuts at: 15290 15290 Size: 17986 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2825 2857 6285 6317 8525 8557 16055 16087 17568 Size: 32 3428 32 2208 32 7498 32 1481 Cuts at: 17568 17600 2825 Size: 32 3211 Fragments arranged by size: 7498 3428 3211 2208 1481 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2186 2326 2822 3224 3400 4003 4324 5659 6871 Size: 140 496 402 176 603 321 1335 1212 Cuts at: 6871 7447 8632 9251 9486 11911 11969 14464 2186 Size: 576 1185 619 235 2425 58 2495 5708 Fragments arranged by size: 5708 2495 2425 1335 1212 1185 619 603 576 496 402 321 235 176 140 58 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 17986 PsiI TTA'TAA Cuts at: 15556 15556 Size: 17986 PspOMI G'GGCC_C Cuts at: 2497 6731 9598 11304 14814 2497 Size: 4234 2867 1706 3510 5669 Fragments arranged by size: 5669 4234 3510 2867 1706 PspXI vC'TCGA_Gb Cuts at: 10784 10784 Size: 17986 PstI C_TGCA'G Cuts at: 830 2145 2680 4665 4863 5772 7420 9297 12264 Size: 1315 535 1985 198 909 1648 1877 2967 Cuts at: 12264 13563 13746 14004 14577 14886 15109 15338 830 Size: 1299 183 258 573 309 223 229 3478 Fragments arranged by size: 3478 2967 1985 1877 1648 1315 1299 909 573 535 309 258 229 223 198 183 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 17986 PvuII CAG'CTG Cuts at: 2887 4364 6400 7903 8915 11948 12556 12718 12772 Size: 1477 2036 1503 1012 3033 608 162 54 Cuts at: 12772 13564 13741 2887 Size: 792 177 7132 Fragments arranged by size: 7132 3033 2036 1503 1477 1012 792 608 177 162 54 SacI G_AGCT'C Cuts at: 2154 5685 10440 2154 Size: 3531 4755 9700 Fragments arranged by size: 9700 4755 3531 SalI G'TCGA_C Cuts at: 1936 9182 15328 1936 Size: 7246 6146 4594 Fragments arranged by size: 7246 6146 4594 SanDI GG'GwC_CC Cuts at: 3224 9251 9486 11969 3224 Size: 6027 235 2483 9241 Fragments arranged by size: 9241 6027 2483 235 SapI GCTCTTCn'nnn_ Cuts at: 3170 10217 15116 3170 Size: 7047 4899 6040 Fragments arranged by size: 7047 6040 4899 SbfI CC_TGCA'GG Cuts at: 7420 15338 7420 Size: 7918 10068 ScaI AGT'ACT Cuts at: 7310 16234 17946 7310 Size: 8924 1712 7350 Fragments arranged by size: 8924 7350 1712 SexAI A'CCwGG_T Cuts at: 5955 6222 6426 7059 9204 11139 11922 5955 Size: 267 204 633 2145 1935 783 12019 Fragments arranged by size: 12019 2145 1935 783 633 267 204 SfcI C'TryA_G Cuts at: 826 1045 2141 2331 2676 4661 4859 5768 6093 Size: 219 1096 190 345 1985 198 909 325 Cuts at: 6093 7416 7982 8294 9293 12260 12378 12484 13015 Size: 1323 566 312 999 2967 118 106 531 Cuts at: 13015 13559 13742 14000 14573 14882 15105 15334 16469 Size: 544 183 258 573 309 223 229 1135 Cuts at: 16469 17150 17341 826 Size: 681 191 1471 Fragments arranged by size: 2967 1985 1471 1323 1135 1096 999 909 681 573 566 544 531 345 325 312 309 258 229 223 219 198 191 190 183 118 106 SfiI GGCCn_nnn'nGGCC Cuts at: 8651 11303 14813 8651 Size: 2652 3510 11824 Fragments arranged by size: 11824 3510 2652 SfoI GGC'GCC Cuts at: 2952 5141 14171 2952 Size: 2189 9030 6767 Fragments arranged by size: 9030 6767 2189 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 17986 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 17986 SmaI CCC'GGG Cuts at: 1872 4134 7163 10592 14453 15313 1872 Size: 2262 3029 3429 3861 860 4545 Fragments arranged by size: 4545 3861 3429 3029 2262 860 SmlI C'TyrA_G Cuts at: 820 1017 1943 2190 4139 4871 5063 7040 10439 Size: 197 926 247 1949 732 192 1977 3399 Cuts at: 10439 10784 12647 14111 14468 16090 16991 17253 820 Size: 345 1863 1464 357 1622 901 262 1553 Fragments arranged by size: 3399 1977 1949 1863 1622 1553 1464 926 901 732 357 345 262 247 197 192 SnaBI TAC'GTA Cuts at: 493 15777 493 Size: 15284 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 17986 SphI G_CATG'C Cuts at: 1132 2395 3889 9883 15344 1132 Size: 1263 1494 5994 5461 3774 Fragments arranged by size: 5994 5461 3774 1494 1263 SspI AAT'ATT Cuts at: 5 52 15910 5 Size: 47 15858 2081 Fragments arranged by size: 15858 2081 47 StuI AGG'CCT Cuts at: 1517 5448 6266 6507 8300 14333 1517 Size: 3931 818 241 1793 6033 5170 Fragments arranged by size: 6033 5170 3931 1793 818 241 StyI C'CwwG_G Cuts at: 513 1065 2157 3066 4548 5655 5781 6009 6376 Size: 552 1092 909 1482 1107 126 228 367 Cuts at: 6376 7515 7785 8199 8370 8383 8937 9402 9594 Size: 1139 270 414 171 13 554 465 192 Cuts at: 9594 9632 9864 10104 10686 10728 11610 11673 12112 Size: 38 232 240 582 42 882 63 439 Cuts at: 12112 13438 14099 14265 14979 15129 15425 513 Size: 1326 661 166 714 150 296 3074 Fragments arranged by size: 3074 1482 1326 1139 1107 1092 909 882 714 661 582 554 552 465 439 414 367 296 270 240 232 228 192 171 166 150 126 63 42 38 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3643 4669 5859 6509 6516 6919 7448 Size: 1255 2063 1026 1190 650 7 403 529 Cuts at: 7448 7670 10484 10954 11590 13663 13812 14443 15201 Size: 222 2814 470 636 2073 149 631 758 Cuts at: 15201 16013 16030 16183 16368 16813 325 Size: 812 17 153 185 445 1498 Fragments arranged by size: 2814 2073 2063 1498 1255 1190 1026 812 758 650 636 631 529 470 445 403 222 185 153 149 17 7 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2398 4366 4473 Size: 274 80 33 51 1443 421 1968 107 Cuts at: 4473 6357 6780 7308 7527 7872 8844 8874 9564 Size: 1884 423 528 219 345 972 30 690 Cuts at: 9564 9789 15138 16232 17944 96 Size: 225 5349 1094 1712 138 Fragments arranged by size: 5349 1968 1884 1712 1443 1094 972 690 528 423 421 345 274 225 219 138 107 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2267 2299 2900 2932 2961 2993 4691 4723 7732 Size: 32 601 32 29 32 1698 32 3009 Cuts at: 7732 7764 8449 8481 8507 8539 11216 11248 12300 Size: 32 685 32 26 32 2677 32 1052 Cuts at: 12300 12332 13589 13621 13659 13691 17946 17978 2267 Size: 32 1257 32 38 32 4255 32 2275 Fragments arranged by size: 4255 3009 2677 2275 1698 1257 1052 685 601 38 32 32 32 32 32 32 32 32 32 32 32 32 29 26 Tth111I GACn'n_nGTC Cuts at: 1316 3683 5339 5957 6428 10447 1316 Size: 2367 1656 618 471 4019 8855 Fragments arranged by size: 8855 4019 2367 1656 618 471 XbaI T'CTAG_A Cuts at: 11266 15322 11266 Size: 4056 13930 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3781 7925 8248 9244 9760 10948 11104 11305 13185 Size: 4144 323 996 516 1188 156 201 1880 Cuts at: 13185 14356 14757 3781 Size: 1171 401 7010 Fragments arranged by size: 7010 4144 1880 1188 1171 996 516 401 323 201 156 XhoI C'TCGA_G Cuts at: 1943 10784 1943 Size: 8841 9145 XmaI C'CCGG_G Cuts at: 1870 4132 7161 10590 14451 15311 1870 Size: 2262 3029 3429 3861 860 4545 Fragments arranged by size: 4545 3861 3429 3029 2262 860 XmnI GAAnn'nnTTC Cuts at: 4232 5105 6371 6549 7821 8007 16115 4232 Size: 873 1266 178 1272 186 8108 6103 Fragments arranged by size: 8108 6103 1272 1266 873 186 178 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 15794 276 Size: 53 83 186 936 14260 2468 Fragments arranged by size: 14260 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BplI FseI PacI PmlI PsrI RsrII SacII SrfI SwaI