(Circular) (Six-base) MAPSORT of: pFN21AB0047.seq Check: 8605 from: 1 to: 10726 pFN21AB0047 10726 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 6706 6706 Size: 10726 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8536 278 Size: 53 83 186 936 7000 2468 Fragments arranged by size: 7000 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 7684 8069 1937 Size: 5747 385 4594 Fragments arranged by size: 5747 4594 385 Acc65I G'GTAC_C Cuts at: 8047 8047 Size: 10726 AclI AA'CG_TT Cuts at: 1563 6675 8853 9226 1563 Size: 5112 2178 373 3063 Fragments arranged by size: 5112 3063 2178 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2339 3072 4643 4830 5166 5450 5654 5925 Size: 707 733 1571 187 336 284 204 271 Cuts at: 5925 6053 6125 6311 6995 7508 7889 8789 10173 Size: 128 72 186 684 513 381 900 1384 Cuts at: 10173 10441 1632 Size: 268 1917 Fragments arranged by size: 1917 1571 1384 900 733 707 684 513 381 336 284 271 268 204 187 186 128 72 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10529 AflIII A'CryG_T Cuts at: 1203 7778 9625 10602 1203 Size: 6575 1847 977 1327 Fragments arranged by size: 6575 1847 1327 977 AgeI A'CCGG_T Cuts at: 9557 9557 Size: 10726 AhdI GACnn_n'nnGTC Cuts at: 3378 4879 9455 3378 Size: 1501 4576 4649 Fragments arranged by size: 4649 4576 1501 AleI CACnn'nnGTG Cuts at: 1166 4616 1166 Size: 3450 7276 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4554 1184 Size: 3370 7356 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4332 4364 6660 6692 4332 Size: 32 2296 32 8366 Fragments arranged by size: 8366 2296 32 32 AlwNI CAG_nnn'CTG Cuts at: 2549 3490 4796 4960 5254 5782 6319 6382 7465 Size: 941 1306 164 294 528 537 63 1083 Cuts at: 7465 8469 10041 2549 Size: 1004 1572 3234 Fragments arranged by size: 3234 1572 1306 1083 1004 941 537 528 294 164 63 ApaI G_GGCC'C Cuts at: 6571 6706 6571 Size: 135 10591 ApaLI G'TGCA_C Cuts at: 8783 9939 8783 Size: 1156 9570 ApoI r'AATT_y Cuts at: 1493 2765 3426 4265 5077 6011 8035 8261 8413 Size: 1272 661 839 812 934 2024 226 152 Cuts at: 8413 10522 1493 Size: 2109 1697 Fragments arranged by size: 2109 2024 1697 1272 934 839 812 661 226 152 AseI AT'TA_AT Cuts at: 160 1032 9280 160 Size: 872 8248 1606 Fragments arranged by size: 8248 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10726 AvaI C'yCGr_G Cuts at: 1870 1943 2024 3213 5066 6564 6572 6899 7014 Size: 73 81 1189 1853 1498 8 327 115 Cuts at: 7014 7508 8051 1870 Size: 494 543 4545 Fragments arranged by size: 4545 1853 1498 1189 543 494 327 115 81 73 8 BamHI G'GATC_C Cuts at: 2029 4168 4218 6108 8056 8423 2029 Size: 2139 50 1890 1948 367 4332 Fragments arranged by size: 4332 2139 1948 1890 367 50 BanI G'GyrC_C Cuts at: 618 943 1160 1787 7926 8047 9502 618 Size: 325 217 627 6139 121 1455 1842 Fragments arranged by size: 6139 1842 1455 627 325 217 121 BanII G_rGCy'C Cuts at: 1391 4803 4993 5340 5721 5889 6571 6653 6706 Size: 3412 190 347 381 168 682 82 53 Cuts at: 6706 6900 7204 7962 8045 1391 Size: 194 304 758 83 4072 Fragments arranged by size: 4072 3412 758 682 381 347 304 194 190 168 83 82 53 BbeI G_GCGC'C Cuts at: 7930 7930 Size: 10726 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2561 2735 3938 4600 5478 6131 6362 Size: 971 662 174 1203 662 878 653 231 Cuts at: 6362 6645 6759 928 Size: 283 114 4895 Fragments arranged by size: 4895 1203 971 878 662 662 653 283 231 174 114 BbvCI CC'TCA_GC Cuts at: 7880 7880 Size: 10726 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5278 5312 8917 8951 1903 Size: 34 3341 34 3605 34 3678 Fragments arranged by size: 3678 3605 3341 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6046 8618 9828 6046 Size: 2572 1210 6944 Fragments arranged by size: 6944 2572 1210 BclI T'GATC_A Cuts at: 1548 3136 6879 1548 Size: 1588 3743 5395 Fragments arranged by size: 5395 3743 1588 BfrBI ATG'CAT Cuts at: 2496 3588 2496 Size: 1092 9634 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4508 6587 6815 Size: 107 122 71 631 605 2836 2079 228 Cuts at: 6815 7556 7832 7920 9337 136 Size: 741 276 88 1417 1525 Fragments arranged by size: 2836 2079 1525 1417 741 631 605 276 228 122 107 88 71 BglII A'GATC_T Cuts at: 10721 10721 Size: 10726 BlpI GC'TnA_GC Cuts at: 8143 8143 Size: 10726 Bme1580I G_kGCm'C Cuts at: 1790 6571 6706 8787 9943 1790 Size: 4781 135 2081 1156 2573 Fragments arranged by size: 4781 2573 2081 1156 135 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 10726 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6205 8011 8457 Size: 416 53 83 399 24 4788 1806 446 Cuts at: 8457 9415 442 Size: 958 1753 Fragments arranged by size: 4788 1806 1753 958 446 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 7418 1056 Size: 6362 4364 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3457 5149 5737 3457 Size: 1692 588 8446 Fragments arranged by size: 8446 1692 588 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2922 4434 4742 5582 5732 5804 6203 6341 Size: 1683 1512 308 840 150 72 399 138 Cuts at: 6341 6371 6605 6746 6761 6812 6932 7031 7340 Size: 30 234 141 15 51 120 99 309 Cuts at: 7340 7487 7574 7595 7775 7790 7976 9386 1239 Size: 147 87 21 180 15 186 1410 2579 Fragments arranged by size: 2579 1683 1512 1410 840 399 309 308 234 186 180 150 147 141 138 120 99 87 72 51 30 21 15 15 Bpu10I CC'TnA_GC Cuts at: 5918 6018 6098 6308 7847 7880 5918 Size: 100 80 210 1539 33 8764 Fragments arranged by size: 8764 1539 210 100 80 33 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2067 5324 5548 5666 6056 7607 8851 9716 10014 Size: 3257 224 118 390 1551 1244 865 298 Cuts at: 10014 2067 Size: 2779 Fragments arranged by size: 3257 2779 1551 1244 865 390 298 224 118 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3011 3598 5905 6825 9389 882 Size: 617 1512 587 2307 920 2564 2219 Fragments arranged by size: 2564 2307 2219 1512 920 617 587 BsaAI yAC'GTr Cuts at: 493 1204 5603 8517 493 Size: 711 4399 2914 2702 Fragments arranged by size: 4399 2914 2702 711 BsaBI GATnn'nnATC Cuts at: 4223 10720 4223 Size: 6497 4229 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 7294 7927 8533 8915 Size: 53 83 186 936 5761 633 606 382 Cuts at: 8915 9550 275 Size: 635 1451 Fragments arranged by size: 5761 1451 936 635 633 606 382 186 83 53 BsaWI w'CCGG_w Cuts at: 9159 9557 9831 9978 10389 9159 Size: 398 274 147 411 9496 Fragments arranged by size: 9496 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2160 2190 5792 5822 1178 Size: 30 165 30 757 30 3602 30 6082 Fragments arranged by size: 6082 3602 757 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4739 4994 5066 5378 5381 5663 5729 6089 Size: 3553 255 72 312 3 282 66 360 Cuts at: 6089 6140 6170 6173 6176 6179 6338 6611 6758 Size: 51 30 3 3 3 159 273 147 Cuts at: 6758 7337 7340 7352 7592 1186 Size: 579 3 12 240 4320 Fragments arranged by size: 4320 3553 579 360 312 282 273 255 240 159 147 72 66 51 30 12 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2549 5589 6735 9929 10425 2549 Size: 3040 1146 3194 496 2850 Fragments arranged by size: 3194 3040 2850 1146 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 7919 1163 Size: 573 6183 3970 Fragments arranged by size: 6183 3970 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3247 5292 7369 8176 8937 9965 Size: 275 186 1246 2045 2077 807 761 1028 Cuts at: 9965 1540 Size: 2301 Fragments arranged by size: 2301 2077 2045 1246 1028 807 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4803 5340 5721 5853 5889 6653 6900 7962 8787 Size: 537 381 132 36 764 247 1062 825 Cuts at: 8787 8872 9943 4803 Size: 85 1071 5586 Fragments arranged by size: 5586 1071 1062 825 764 537 381 247 132 85 36 BsmI GAATG_Cn' Cuts at: 3653 8237 8330 3653 Size: 4584 93 6049 Fragments arranged by size: 6049 4584 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5279 7997 5279 Size: 2718 8008 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4803 4993 5340 5721 5853 5889 6571 Size: 399 3013 190 347 381 132 36 682 Cuts at: 6571 6653 6706 6900 7204 7962 8045 8787 8872 Size: 82 53 194 304 758 83 742 85 Cuts at: 8872 9943 1391 Size: 1071 2174 Fragments arranged by size: 3013 2174 1071 758 742 682 399 381 347 304 194 190 132 85 83 82 53 36 BspEI T'CCGG_A Cuts at: 10389 10389 Size: 10726 BspHI T'CATG_A Cuts at: 2446 3949 6645 8615 10345 2446 Size: 1503 2696 1970 1730 2827 Fragments arranged by size: 2827 2696 1970 1730 1503 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3616 5304 5821 5866 6706 6843 7657 8081 Size: 2772 1688 517 45 840 137 814 424 Cuts at: 8081 844 Size: 3489 Fragments arranged by size: 3489 2772 1688 840 814 517 424 137 45 BsrBI CCG'CTC Cuts at: 1394 1823 3325 4735 6508 7471 8613 1394 Size: 429 1502 1410 1773 963 1142 3507 Fragments arranged by size: 3507 1773 1502 1410 1142 963 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2217 3036 5118 9221 9395 65 Size: 1183 969 819 2082 4103 174 1396 Fragments arranged by size: 4103 2082 1396 1183 969 819 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7875 7923 9370 9557 1700 Size: 110 6065 48 1447 187 2869 Fragments arranged by size: 6065 2869 1447 187 110 48 BsrGI T'GTAC_A Cuts at: 96 2427 4377 96 Size: 2331 1950 6445 Fragments arranged by size: 6445 2331 1950 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10726 BssSI C'ACGA_G Cuts at: 2841 4145 7089 8786 9798 2841 Size: 1304 2944 1697 1012 3769 Fragments arranged by size: 3769 2944 1697 1304 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2689 3290 4835 5002 794 Size: 1895 601 1545 167 6518 Fragments arranged by size: 6518 1895 1545 601 167 BstBI TT'CG_AA Cuts at: 9530 9530 Size: 10726 BstEII G'GTnAC_C Cuts at: 1184 4493 1184 Size: 3309 7417 BstXI CCAn_nnnn'nTGG Cuts at: 3188 4090 5983 3188 Size: 902 1893 7931 Fragments arranged by size: 7931 1893 902 BstYI r'GATC_y Cuts at: 1972 2029 3574 4168 4218 4284 6108 8056 8423 Size: 57 1545 594 50 66 1824 1948 367 Cuts at: 8423 8808 8825 10277 10363 10375 10721 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1948 1824 1545 1452 594 385 367 346 86 66 57 50 17 12 Bsu36I CC'TnA_GG Cuts at: 2637 2637 Size: 10726 BtgI C'CryG_G Cuts at: 513 1065 2004 2131 3021 5580 6768 513 Size: 552 939 127 890 2559 1188 4471 Fragments arranged by size: 4471 2559 1188 939 890 552 127 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3332 487 Size: 683 56 2106 7881 Fragments arranged by size: 7881 2106 683 56 BtsI GCAGTG_nn' Cuts at: 4601 8242 9036 9056 4601 Size: 3641 794 20 6271 Fragments arranged by size: 6271 3641 794 20 ClaI AT'CG_AT Cuts at: 1554 3561 1554 Size: 2007 8719 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10691 DraI TTT'AAA Cuts at: 4449 8030 4449 Size: 3581 7145 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2060 5876 9733 809 Size: 507 744 3816 3857 1802 Fragments arranged by size: 3857 3816 1802 744 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3244 3843 4500 Size: 54 1425 50 275 18 1414 599 657 Cuts at: 4500 5172 5352 5385 7921 8173 9062 10691 8 Size: 672 180 33 2536 252 889 1629 43 Fragments arranged by size: 2536 1629 1425 1414 889 672 657 599 275 252 180 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 3244 8173 1537 Size: 275 1432 4929 4090 Fragments arranged by size: 4929 4090 1432 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2369 3051 4145 4668 4752 4812 4854 5043 Size: 1016 682 1094 523 84 60 42 189 Cuts at: 5043 5142 6117 6468 6473 6498 6855 7139 7329 Size: 99 975 351 5 25 357 284 190 Cuts at: 7329 7436 7912 8656 1353 Size: 107 476 744 3423 Fragments arranged by size: 3423 1094 1016 975 744 682 523 476 357 351 284 190 189 107 99 84 60 42 25 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5948 6920 7253 9313 9687 9833 Size: 1064 138 4359 972 333 2060 374 146 Cuts at: 9833 387 Size: 1280 Fragments arranged by size: 4359 2060 1280 1064 972 374 333 146 138 EcoICRI GAG'CTC Cuts at: 4801 5338 5719 5887 6651 6898 7960 4801 Size: 537 381 168 764 247 1062 7567 Fragments arranged by size: 7567 1062 764 537 381 247 168 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2339 2922 3072 4434 4643 4742 4830 Size: 393 707 583 150 1362 209 99 88 Cuts at: 4830 5166 5450 5582 5654 5732 5804 5925 6053 Size: 336 284 132 72 78 72 121 128 Cuts at: 6053 6125 6203 6311 6341 6371 6605 6746 6761 Size: 72 78 108 30 30 234 141 15 Cuts at: 6761 6812 6932 6995 7031 7340 7487 7508 7574 Size: 51 120 63 36 309 147 21 66 Cuts at: 7574 7595 7775 7790 7889 7976 8789 9386 10173 Size: 21 180 15 99 87 813 597 787 Cuts at: 10173 10441 1239 Size: 268 1524 Fragments arranged by size: 1524 1362 813 787 707 597 583 393 336 309 284 268 234 209 180 150 147 141 132 128 121 120 108 99 99 88 87 78 78 72 72 72 66 63 51 36 30 30 21 21 15 15 EcoNI CCTnn'n_nnAGG Cuts at: 5828 6374 5828 Size: 546 10180 EcoO109I rG'GnC_Cy Cuts at: 4957 5779 5821 6304 6568 6703 6732 6979 7843 Size: 822 42 483 264 135 29 247 864 Cuts at: 7843 4957 Size: 7840 Fragments arranged by size: 7840 864 822 483 264 247 135 42 29 EcoRI G'AATT_C Cuts at: 4265 8035 8413 4265 Size: 3770 378 6578 Fragments arranged by size: 6578 3770 378 EcoRV GAT'ATC Cuts at: 4462 4462 Size: 10726 FspI TGC'GCA Cuts at: 3168 9232 3168 Size: 6064 4662 HaeII r_GCGC'y Cuts at: 1678 6319 6418 6938 7260 7434 7930 9873 1678 Size: 4641 99 520 322 174 496 1943 2531 Fragments arranged by size: 4641 2531 1943 520 496 322 174 99 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2504 2536 2735 2767 3050 Size: 32 135 32 932 32 199 32 283 Cuts at: 3050 3082 3364 3396 4081 4113 4269 4276 4301 Size: 32 282 32 685 32 156 7 25 Cuts at: 4301 4308 4865 4897 5465 5497 5957 5989 7099 Size: 7 557 32 568 32 460 32 1110 Cuts at: 7099 7131 9367 9399 9441 9473 1373 Size: 32 2236 32 42 32 2626 Fragments arranged by size: 2626 2236 1110 932 685 568 557 460 283 282 199 156 135 42 32 32 32 32 32 32 32 32 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 2218 6799 7685 7999 8070 8316 Size: 988 281 280 4581 886 314 71 246 Cuts at: 8316 669 Size: 3079 Fragments arranged by size: 4581 3079 988 886 314 281 280 246 71 HindIII A'AGCT_T Cuts at: 748 2298 3141 6284 6782 748 Size: 1550 843 3143 498 4692 Fragments arranged by size: 4692 3143 1550 843 498 HpaI GTT'AAC Cuts at: 8316 8316 Size: 10726 KasI G'GCGC_C Cuts at: 7926 7926 Size: 10726 KpnI G_GTAC'C Cuts at: 8051 8051 Size: 10726 MfeI C'AATT_G Cuts at: 6255 7169 8325 6255 Size: 914 1156 8656 Fragments arranged by size: 8656 1156 914 MluI A'CGCG_T Cuts at: 7778 10602 7778 Size: 2824 7902 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6086 6453 7989 9840 10024 1305 Size: 4781 367 1536 1851 184 2007 Fragments arranged by size: 4781 2007 1851 1536 367 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3845 4502 5174 5354 5387 Size: 54 1425 343 2013 657 672 180 33 Cuts at: 5387 10 Size: 5349 Fragments arranged by size: 5349 2013 1425 672 657 343 180 54 33 MslI CAynn'nnrTG Cuts at: 518 1166 2466 3995 4616 8684 9043 9202 518 Size: 648 1300 1529 621 4068 359 159 2042 Fragments arranged by size: 4068 2042 1529 1300 648 621 359 159 MspA1I CmG'CkG Cuts at: 1606 3490 4558 5008 5140 5350 5731 6118 6463 Size: 1884 1068 450 132 210 381 387 345 Cuts at: 6463 6739 6931 7318 7573 7753 7810 7933 8142 Size: 276 192 387 255 180 57 123 209 Cuts at: 8142 8819 9967 10212 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1884 1148 1068 677 450 387 387 381 345 276 255 245 210 209 192 180 132 123 57 NaeI GCC'GGC Cuts at: 7925 7925 Size: 10726 NarI GG'CG_CC Cuts at: 7927 7927 Size: 10726 NcoI C'CATG_G Cuts at: 513 1065 2004 2131 3021 513 Size: 552 939 127 890 8218 Fragments arranged by size: 8218 939 890 552 127 NdeI CA'TA_TG Cuts at: 387 387 Size: 10726 NgoMIV G'CCGG_C Cuts at: 7923 7923 Size: 10726 NheI G'CTAG_C Cuts at: 1052 7414 1052 Size: 6362 4364 NotI GC'GGCC_GC Cuts at: 8173 8173 Size: 10726 NsiI A_TGCA'T Cuts at: 2498 3590 2498 Size: 1092 9634 NspI r_CATG'y Cuts at: 1132 1229 2289 2500 4045 6805 8084 9629 1132 Size: 97 1060 211 1545 2760 1279 1545 2229 Fragments arranged by size: 2760 2229 1545 1545 1279 1060 211 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 10726 PciI A'CATG_T Cuts at: 9625 9625 Size: 10726 PflMI CCAn_nnn'nTGG Cuts at: 1108 6319 7465 1108 Size: 5211 1146 4369 Fragments arranged by size: 5211 4369 1146 PfoI T'CCnGG_A Cuts at: 2986 3012 3405 4209 4260 5679 5880 2986 Size: 26 393 804 51 1419 201 7832 Fragments arranged by size: 7832 1419 804 393 201 51 26 PmeI GTTT'AAAC Cuts at: 8030 8030 Size: 10726 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4401 4433 8795 8827 10308 10340 4401 Size: 32 4362 32 1481 32 4787 Fragments arranged by size: 4787 4362 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4957 5821 6732 6979 4957 Size: 864 911 247 8704 Fragments arranged by size: 8704 911 864 247 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10726 PsiI TTA'TAA Cuts at: 2378 8296 2378 Size: 5918 4808 PspOMI G'GGCC_C Cuts at: 6567 6702 6567 Size: 135 10591 PspXI vC'TCGA_Gb Cuts at: 2024 6572 6899 7508 2024 Size: 4548 327 609 5242 Fragments arranged by size: 5242 4548 609 327 PstI C_TGCA'G Cuts at: 830 3486 3582 5007 5301 5322 5349 5376 5829 Size: 2656 96 1425 294 21 27 27 453 Cuts at: 5829 5955 6207 6375 7503 8078 830 Size: 126 252 168 1128 575 3478 Fragments arranged by size: 3478 2656 1425 1128 575 453 294 252 168 126 96 27 27 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10726 PvuII CAG'CTG Cuts at: 3490 5008 5140 5350 5731 6118 6463 6739 7318 Size: 1518 132 210 381 387 345 276 579 Cuts at: 7318 7573 7753 7933 3490 Size: 255 180 180 6283 Fragments arranged by size: 6283 1518 579 387 381 345 276 255 210 180 180 132 SacI G_AGCT'C Cuts at: 4803 5340 5721 5889 6653 6900 7962 4803 Size: 537 381 168 764 247 1062 7567 Fragments arranged by size: 7567 1062 764 537 381 247 168 SalI G'TCGA_C Cuts at: 1936 7683 8068 1936 Size: 5747 385 4594 Fragments arranged by size: 5747 4594 385 SapI GCTCTTCn'nnn_ Cuts at: 4668 6473 6498 4668 Size: 1805 25 8896 Fragments arranged by size: 8896 1805 25 SbfI CC_TGCA'GG Cuts at: 5301 8078 5301 Size: 2777 7949 ScaI AGT'ACT Cuts at: 8974 10686 8974 Size: 1712 9014 SfcI C'TryA_G Cuts at: 826 1045 2953 3095 3482 3578 5003 5297 5318 Size: 219 1908 142 387 96 1425 294 21 Cuts at: 5318 5345 5372 5825 5951 6047 6203 6371 6942 Size: 27 27 453 126 96 156 168 571 Cuts at: 6942 7388 7499 8074 9209 9890 10081 826 Size: 446 111 575 1135 681 191 1471 Fragments arranged by size: 1908 1471 1425 1135 681 575 571 453 446 387 294 219 191 168 156 142 126 111 96 96 27 27 21 SfiI GGCCn_nnn'nGGCC Cuts at: 4508 4508 Size: 10726 SfoI GGC'GCC Cuts at: 7928 7928 Size: 10726 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10726 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10726 SmaI CCC'GGG Cuts at: 1872 6566 8053 1872 Size: 4694 1487 4545 Fragments arranged by size: 4694 4545 1487 SmlI C'TyrA_G Cuts at: 820 1017 1943 2024 2082 5066 5339 5527 5645 Size: 197 926 81 58 2984 273 188 118 Cuts at: 5645 6035 6572 6899 7508 7586 8830 9731 9993 Size: 390 537 327 609 78 1244 901 262 Cuts at: 9993 820 Size: 1553 Fragments arranged by size: 2984 1553 1244 926 901 609 537 390 327 273 262 197 188 118 81 78 58 SnaBI TAC'GTA Cuts at: 493 5603 8517 493 Size: 5110 2914 2702 Fragments arranged by size: 5110 2914 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10726 SphI G_CATG'C Cuts at: 1132 2500 8084 1132 Size: 1368 5584 3774 Fragments arranged by size: 5584 3774 1368 SrfI GCCC'GGGC Cuts at: 6566 6566 Size: 10726 SspI AAT'ATT Cuts at: 5 52 2416 2632 3109 3910 8650 5 Size: 47 2364 216 477 801 4740 2081 Fragments arranged by size: 4740 2364 2081 801 477 216 47 StuI AGG'CCT Cuts at: 1517 4334 4511 1517 Size: 2817 177 7732 Fragments arranged by size: 7732 2817 177 StyI C'CwwG_G Cuts at: 513 1065 2004 2131 2259 3021 5895 6591 7599 Size: 552 939 127 128 762 2874 696 1008 Cuts at: 7599 8165 513 Size: 566 3074 Fragments arranged by size: 3074 2874 1008 939 762 696 566 552 128 127 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5306 8753 8770 8923 9108 9553 325 Size: 1255 3726 3447 17 153 185 445 1498 Fragments arranged by size: 3726 3447 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2427 3625 4377 Size: 274 80 33 51 1443 450 1198 752 Cuts at: 4377 8972 10684 96 Size: 4595 1712 138 Fragments arranged by size: 4595 1712 1443 1198 752 450 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4164 4196 5792 5824 10686 10718 4164 Size: 32 1596 32 4862 32 4172 Fragments arranged by size: 4862 4172 1596 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 10726 XbaI T'CTAG_A Cuts at: 5761 8062 5761 Size: 2301 8425 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2897 6178 6661 6787 7390 7549 2897 Size: 3281 483 126 603 159 6074 Fragments arranged by size: 6074 3281 603 483 159 126 XhoI C'TCGA_G Cuts at: 1943 2024 5066 6572 6899 7508 1943 Size: 81 3042 1506 327 609 5161 Fragments arranged by size: 5161 3042 1506 609 327 81 XmaI C'CCGG_G Cuts at: 1870 6564 8051 1870 Size: 4694 1487 4545 Fragments arranged by size: 4694 4545 1487 XmnI GAAnn'nnTTC Cuts at: 3060 3109 4656 4863 7164 8855 3060 Size: 49 1547 207 2301 1691 4931 Fragments arranged by size: 4931 2301 1691 1547 207 49 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8534 276 Size: 53 83 186 936 7000 2468 Fragments arranged by size: 7000 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BsiWI BstZ17I DraIII FalI FseI FspAI NruI PacI PmlI PsrI RsrII SacII SanDI SexAI SwaI