(Circular) (Six-base) MAPSORT of: pFN21AA1997.seq Check: 2791 from: 1 to: 17626 pFN21AA1997 17626 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3193 3193 Size: 17626 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 15436 278 Size: 53 83 186 936 13900 2468 Fragments arranged by size: 13900 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6335 6956 9039 14623 14969 1937 Size: 4398 621 2083 5584 346 4594 Fragments arranged by size: 5584 4594 4398 2083 621 346 Acc65I G'GTAC_C Cuts at: 14947 14947 Size: 17626 AclI AA'CG_TT Cuts at: 1563 13138 15753 16126 1563 Size: 11575 2615 373 3063 Fragments arranged by size: 11575 3063 2615 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2274 2390 4995 5120 6386 6669 6888 9386 Size: 642 116 2605 125 1266 283 219 2498 Cuts at: 9386 10085 10182 10892 11384 11394 11445 11505 13502 Size: 699 97 710 492 10 51 60 1997 Cuts at: 13502 13582 13808 13839 13856 15689 17073 17341 1632 Size: 80 226 31 17 1833 1384 268 1917 Fragments arranged by size: 2605 2498 1997 1917 1833 1384 1266 710 699 642 492 283 268 226 219 125 116 97 80 60 51 31 17 10 AfeI AGC'GCT Cuts at: 2927 4154 2927 Size: 1227 16399 AflII C'TTAA_G Cuts at: 820 1017 8732 10256 820 Size: 197 7715 1524 8190 Fragments arranged by size: 8190 7715 1524 197 AflIII A'CryG_T Cuts at: 1203 6920 10090 10633 12546 12801 16525 17502 1203 Size: 5717 3170 543 1913 255 3724 977 1327 Fragments arranged by size: 5717 3724 3170 1913 1327 977 543 255 AgeI A'CCGG_T Cuts at: 16457 16457 Size: 17626 AhdI GACnn_n'nnGTC Cuts at: 9706 16355 9706 Size: 6649 10977 AleI CACnn'nnGTG Cuts at: 1166 2101 1166 Size: 935 16691 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2165 8915 14825 1184 Size: 981 6750 5910 3985 Fragments arranged by size: 6750 5910 3985 981 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2775 2807 12618 12650 2775 Size: 32 9811 32 7751 Fragments arranged by size: 9811 7751 32 32 AlwNI CAG_nnn'CTG Cuts at: 2098 8995 10805 11468 15369 16941 2098 Size: 6897 1810 663 3901 1572 2783 Fragments arranged by size: 6897 3901 2783 1810 1572 663 ApaLI G'TGCA_C Cuts at: 8961 12408 15683 16839 8961 Size: 3447 3275 1156 9748 Fragments arranged by size: 9748 3447 3275 1156 ApoI r'AATT_y Cuts at: 1493 2483 3231 3399 3830 3958 4315 4657 5054 Size: 990 748 168 431 128 357 342 397 Cuts at: 5054 5162 5391 5486 6025 6199 6254 6641 6849 Size: 108 229 95 539 174 55 387 208 Cuts at: 6849 6947 7229 7348 7362 8203 8564 8624 9175 Size: 98 282 119 14 841 361 60 551 Cuts at: 9175 9399 9452 9874 10317 10360 10537 11010 11319 Size: 224 53 422 443 43 177 473 309 Cuts at: 11319 11444 11714 12098 12450 13519 13673 13751 13962 Size: 125 270 384 352 1069 154 78 211 Cuts at: 13962 14093 14161 14600 14935 15161 15313 17422 1493 Size: 131 68 439 335 226 152 2109 1697 Fragments arranged by size: 2109 1697 1069 990 841 748 551 539 473 443 439 431 422 397 387 384 361 357 352 342 335 309 282 270 229 226 224 211 208 177 174 168 154 152 131 128 125 119 108 98 95 78 68 60 55 53 43 14 AseI AT'TA_AT Cuts at: 160 1032 4734 5837 8574 10574 11532 12540 16180 Size: 872 3702 1103 2737 2000 958 1008 3640 Cuts at: 16180 160 Size: 1606 Fragments arranged by size: 3702 3640 2737 2000 1606 1103 1008 958 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 17626 AvaI C'yCGr_G Cuts at: 1870 1943 3081 14951 1870 Size: 73 1138 11870 4545 Fragments arranged by size: 11870 4545 1138 73 AvrII C'CTAG_G Cuts at: 5663 5663 Size: 17626 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4833 4866 5709 5742 9416 9449 4833 Size: 33 843 33 3674 33 13010 Fragments arranged by size: 13010 3674 843 33 33 33 BamHI G'GATC_C Cuts at: 6751 9545 14956 15323 6751 Size: 2794 5411 367 9054 Fragments arranged by size: 9054 5411 2794 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3390 13730 14947 16402 618 Size: 325 217 627 1603 10340 1217 1455 1842 Fragments arranged by size: 10340 1842 1603 1455 1217 627 325 217 BanII G_rGCy'C Cuts at: 1391 3141 9689 9739 10242 11617 12595 13588 14945 Size: 1750 6548 50 503 1375 978 993 1357 Cuts at: 14945 1391 Size: 4072 Fragments arranged by size: 6548 4072 1750 1375 1357 993 978 503 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4757 4983 5126 5451 5856 6674 6876 Size: 971 2858 226 143 325 405 818 202 Cuts at: 6876 7132 7411 7619 8751 9241 9250 10188 10288 Size: 256 279 208 1132 490 9 938 100 Cuts at: 10288 11145 11382 928 Size: 857 237 7172 Fragments arranged by size: 7172 2858 1132 971 938 857 818 490 405 325 279 256 237 226 208 202 143 100 9 BbvCI CC'TCA_GC Cuts at: 7578 7578 Size: 17626 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5366 5400 12713 12747 15817 15851 1903 Size: 34 3429 34 7313 34 3070 34 3678 Fragments arranged by size: 7313 3678 3429 3070 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2519 8680 12766 15518 16728 2519 Size: 6161 4086 2752 1210 3417 Fragments arranged by size: 6161 4086 3417 2752 1210 BclI T'GATC_A Cuts at: 1548 2725 3886 6220 6913 10179 10818 12460 12703 Size: 1177 1161 2334 693 3266 639 1642 243 Cuts at: 12703 12838 14031 1548 Size: 135 1193 5143 Fragments arranged by size: 5143 3266 2334 1642 1193 1177 1161 693 639 243 135 BfrBI ATG'CAT Cuts at: 3413 4231 4235 10018 12742 14774 3413 Size: 818 4 5783 2724 2032 6265 Fragments arranged by size: 6265 5783 2724 2032 818 4 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 16237 136 Size: 107 122 71 631 605 14565 1525 Fragments arranged by size: 14565 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3568 3653 4537 5139 8365 12031 13687 17621 3568 Size: 85 884 602 3226 3666 1656 3934 3573 Fragments arranged by size: 3934 3666 3573 3226 1656 884 602 85 BlpI GC'TnA_GC Cuts at: 4189 6274 11241 15043 4189 Size: 2085 4967 3802 6772 Fragments arranged by size: 6772 4967 3802 2085 Bme1580I G_kGCm'C Cuts at: 1790 5389 5767 8653 8965 11837 12412 13318 14449 Size: 3599 378 2886 312 2872 575 906 1131 Cuts at: 14449 15687 16843 1790 Size: 1238 1156 2573 Fragments arranged by size: 3599 2886 2872 2573 1238 1156 1131 906 575 378 312 BmgBI CAC'GTC Cuts at: 1318 5132 1318 Size: 3814 13812 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4879 6576 6883 Size: 416 53 83 399 24 3462 1697 307 Cuts at: 6883 7092 8235 9248 12900 14473 15357 16315 442 Size: 209 1143 1013 3652 1573 884 958 1753 Fragments arranged by size: 3652 3462 1753 1697 1573 1143 1013 958 884 416 399 307 209 83 53 24 BmtI G_CTAG'C Cuts at: 1056 12305 1056 Size: 11249 6377 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 13349 14821 13349 Size: 1472 16154 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4295 5607 6800 8649 9249 9966 10779 13488 Size: 3056 1312 1193 1849 600 717 813 2709 Cuts at: 13488 16286 1239 Size: 2798 2579 Fragments arranged by size: 3056 2798 2709 2579 1849 1312 1193 813 717 600 Bpu10I CC'TnA_GC Cuts at: 3934 7578 8906 13551 3934 Size: 3644 1328 4645 8009 Fragments arranged by size: 8009 4645 3644 1328 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6564 7017 8124 9809 10160 11498 11729 12027 12330 Size: 453 1107 1685 351 1338 231 298 303 Cuts at: 12330 15751 16616 16914 6564 Size: 3421 865 298 7276 Fragments arranged by size: 7276 3421 1685 1338 1107 865 453 351 303 298 298 231 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 16289 882 Size: 617 14790 2219 Fragments arranged by size: 14790 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5798 12836 15417 493 Size: 711 4594 7038 2581 2702 Fragments arranged by size: 7038 4594 2702 2581 711 BsaBI GATnn'nnATC Cuts at: 2271 2730 4911 5316 8220 11078 11353 12477 14691 Size: 459 2181 405 2904 2858 275 1124 2214 Cuts at: 14691 17620 2271 Size: 2929 2277 Fragments arranged by size: 2929 2904 2858 2277 2214 2181 1124 459 405 275 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 15433 15815 16450 275 Size: 53 83 186 936 13900 382 635 1451 Fragments arranged by size: 13900 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 8928 12145 13051 14791 16059 16457 16731 16878 17289 Size: 3217 906 1740 1268 398 274 147 411 Cuts at: 17289 8928 Size: 9265 Fragments arranged by size: 9265 3217 1740 1268 906 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4268 4298 6438 6468 9899 Size: 30 165 30 2865 30 2140 30 3431 Cuts at: 9899 9929 10421 10451 1178 Size: 30 492 30 8353 Fragments arranged by size: 8353 3431 2865 2140 492 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2144 8652 11411 11740 14181 1186 Size: 958 6508 2759 329 2441 4631 Fragments arranged by size: 6508 4631 2759 2441 958 329 BseYI C'CCAG_C Cuts at: 2432 12873 13697 14723 16829 17325 2432 Size: 10441 824 1026 2106 496 2733 Fragments arranged by size: 10441 2733 2106 1026 824 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2183 3759 6606 11247 1163 Size: 573 447 1576 2847 4641 7542 Fragments arranged by size: 7542 4641 2847 1576 573 447 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6076 14203 15076 15837 16865 1540 Size: 275 186 4075 8127 873 761 1028 2301 Fragments arranged by size: 8127 4075 2301 1028 873 761 275 186 BsiHKAI G_wGCw'C Cuts at: 8119 8965 9739 11617 12412 12595 13588 14742 15687 Size: 846 774 1878 795 183 993 1154 945 Cuts at: 15687 15772 16843 8119 Size: 85 1071 8902 Fragments arranged by size: 8902 1878 1154 1071 993 945 846 795 774 183 85 BsmI GAATG_Cn' Cuts at: 3263 4428 6278 7681 9131 9495 11242 11569 15137 Size: 1165 1850 1403 1450 364 1747 327 3568 Cuts at: 15137 15230 3263 Size: 93 5659 Fragments arranged by size: 5659 3568 1850 1747 1450 1403 1165 364 327 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5654 12019 5654 Size: 6365 11261 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3141 5389 5767 8119 8653 8965 9689 Size: 399 1351 2248 378 2352 534 312 724 Cuts at: 9689 9739 10242 11617 11837 12412 12595 13318 13588 Size: 50 503 1375 220 575 183 723 270 Cuts at: 13588 14449 14742 14945 15687 15772 16843 1391 Size: 861 293 203 742 85 1071 2174 Fragments arranged by size: 2352 2248 2174 1375 1351 1071 861 742 724 723 575 534 503 399 378 312 293 270 220 203 183 85 50 BspEI T'CCGG_A Cuts at: 14791 17289 14791 Size: 2498 15128 BspHI T'CATG_A Cuts at: 3025 8293 8557 10371 15515 17245 3025 Size: 5268 264 1814 5144 1730 3406 Fragments arranged by size: 5268 5144 3406 1814 1730 264 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3058 3193 6027 7828 14981 844 Size: 2214 135 2834 1801 7153 3489 Fragments arranged by size: 7153 3489 2834 2214 1801 135 BsrBI CCG'CTC Cuts at: 1394 1823 10025 15513 1394 Size: 429 8202 5488 3507 Fragments arranged by size: 8202 5488 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3819 6083 6435 13327 16121 16295 65 Size: 1183 2571 2264 352 6892 2794 174 1396 Fragments arranged by size: 6892 2794 2571 2264 1396 1183 352 174 BsrFI r'CCGG_y Cuts at: 1700 1810 16270 16457 1700 Size: 110 14460 187 2869 Fragments arranged by size: 14460 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4263 4639 10156 13741 13756 96 Size: 4167 376 5517 3585 15 3966 Fragments arranged by size: 5517 4167 3966 3585 376 15 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 17626 BssSI C'ACGA_G Cuts at: 2502 8652 15686 16698 2502 Size: 6150 7034 1012 3430 Fragments arranged by size: 7034 6150 3430 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3821 10330 10601 10805 12407 794 Size: 3027 6509 271 204 1602 6013 Fragments arranged by size: 6509 6013 3027 1602 271 204 BstBI TT'CG_AA Cuts at: 16430 16430 Size: 17626 BstEII G'GTnAC_C Cuts at: 1184 14845 1184 Size: 13661 3965 BstXI CCAn_nnnn'nTGG Cuts at: 5631 7045 9124 5631 Size: 1414 2079 14133 Fragments arranged by size: 14133 2079 1414 BstYI r'GATC_y Cuts at: 1972 2827 3568 3653 4537 5139 6751 8365 9416 Size: 855 741 85 884 602 1612 1614 1051 Cuts at: 9416 9545 11848 12031 13687 14072 14210 14410 14956 Size: 129 2303 183 1656 385 138 200 546 Cuts at: 14956 15323 15708 15725 17177 17263 17275 17621 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 2303 1977 1656 1614 1612 1452 1051 884 855 741 602 546 385 385 367 346 200 183 138 129 86 85 17 12 BstZ17I GTA'TAC Cuts at: 6957 9040 6957 Size: 2083 15543 Bsu36I CC'TnA_GG Cuts at: 2156 13650 2156 Size: 11494 6132 BtgI C'CryG_G Cuts at: 513 1065 2004 2557 2942 6076 8834 12879 13071 Size: 552 939 553 385 3134 2758 4045 192 Cuts at: 13071 13266 513 Size: 195 4873 Fragments arranged by size: 4873 4045 3134 2758 939 553 552 385 195 192 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2538 487 Size: 683 56 1312 15575 Fragments arranged by size: 15575 1312 683 56 BtsI GCAGTG_nn' Cuts at: 5832 9979 12412 13118 13431 14580 15142 15936 15956 Size: 4147 2433 706 313 1149 562 794 20 Cuts at: 15956 5832 Size: 7502 Fragments arranged by size: 7502 4147 2433 1149 794 706 562 313 20 ClaI AT'CG_AT Cuts at: 1554 7161 1554 Size: 5607 12019 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3330 3365 8923 8958 14556 14591 14867 Size: 35 2766 35 5558 35 5598 35 276 Cuts at: 14867 14902 529 Size: 35 3253 Fragments arranged by size: 5598 5558 3253 2766 276 35 35 35 35 35 DraI TTT'AAA Cuts at: 2599 4663 4728 5167 5754 6030 6463 7510 11209 Size: 2064 65 439 587 276 433 1047 3699 Cuts at: 11209 13512 14930 2599 Size: 2303 1418 5295 Fragments arranged by size: 5295 3699 2303 2064 1418 1047 587 439 433 276 65 DraIII CAC_nnn'GTG Cuts at: 5771 5771 Size: 17626 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6164 16633 809 Size: 507 4848 10469 1802 Fragments arranged by size: 10469 4848 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5215 5436 6073 Size: 54 1425 50 275 18 3385 221 637 Cuts at: 6073 15073 15962 17591 8 Size: 9000 889 1629 43 Fragments arranged by size: 9000 3385 1629 1425 889 637 275 221 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6073 15073 1537 Size: 275 4261 9000 4090 Fragments arranged by size: 9000 4261 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2042 2994 3054 4409 5328 5403 7842 8352 Size: 689 952 60 1355 919 75 2439 510 Cuts at: 8352 8787 11607 12327 13404 13601 14496 14556 15556 Size: 435 2820 720 1077 197 895 60 1000 Cuts at: 15556 1353 Size: 3423 Fragments arranged by size: 3423 2820 2439 1355 1077 1000 952 919 895 720 689 510 435 197 75 60 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6644 12092 16213 16587 16733 387 Size: 1064 138 5055 5448 4121 374 146 1280 Fragments arranged by size: 5448 5055 4121 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 9737 11615 12593 13586 9737 Size: 1878 978 993 13777 Fragments arranged by size: 13777 1878 993 978 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2274 2390 4295 4995 5120 5607 6386 Size: 393 642 116 1905 700 125 487 779 Cuts at: 6386 6669 6800 6888 8649 9249 9386 9966 10085 Size: 283 131 88 1761 600 137 580 119 Cuts at: 10085 10182 10779 10892 11384 11394 11445 11505 13488 Size: 97 597 113 492 10 51 60 1983 Cuts at: 13488 13502 13582 13808 13839 13856 15689 16286 17073 Size: 14 80 226 31 17 1833 597 787 Cuts at: 17073 17341 1239 Size: 268 1524 Fragments arranged by size: 1983 1905 1833 1761 1524 787 779 700 642 600 597 597 580 492 487 393 283 268 226 137 131 125 119 116 113 97 88 80 60 51 31 17 14 10 EcoNI CCTnn'n_nnAGG Cuts at: 2999 5480 9140 2999 Size: 2481 3660 11485 Fragments arranged by size: 11485 3660 2481 EcoO109I rG'GnC_Cy Cuts at: 4371 7209 4371 Size: 2838 14788 EcoRI G'AATT_C Cuts at: 3399 3958 8203 8564 10317 11319 12098 14161 14935 Size: 559 4245 361 1753 1002 779 2063 774 Cuts at: 14935 15313 3399 Size: 378 5712 Fragments arranged by size: 5712 4245 2063 1753 1002 779 774 559 378 361 EcoRV GAT'ATC Cuts at: 5374 6148 8051 9085 5374 Size: 774 1903 1034 13915 Fragments arranged by size: 13915 1903 1034 774 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3348 7036 7858 11219 12338 12443 12529 3348 Size: 3688 822 3361 1119 105 86 8445 Fragments arranged by size: 8445 3688 3361 1119 822 105 86 FspI TGC'GCA Cuts at: 16132 16132 Size: 17626 HaeII r_GCGC'y Cuts at: 1678 2929 4156 9604 16773 1678 Size: 1251 1227 5448 7169 2531 Fragments arranged by size: 7169 5448 2531 1251 1227 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2633 2665 3602 3617 3634 Size: 32 135 32 1061 32 937 15 17 Cuts at: 3634 3638 3649 3670 3889 3921 4750 4782 5059 Size: 4 11 21 219 32 829 32 277 Cuts at: 5059 5091 5131 5163 5913 5945 5963 5995 6116 Size: 32 40 32 750 32 18 32 121 Cuts at: 6116 6148 6343 6375 6395 6427 7154 7186 8135 Size: 32 195 32 20 32 727 32 949 Cuts at: 8135 8167 8182 8214 9671 9692 9703 9724 9899 Size: 32 15 32 1457 21 11 21 175 Cuts at: 9899 9931 10421 10453 12016 12048 12974 13006 14023 Size: 32 490 32 1563 32 926 32 1017 Cuts at: 14023 14055 16267 16299 16341 16373 1373 Size: 32 2212 32 42 32 2626 Fragments arranged by size: 2626 2212 1563 1457 1061 1017 949 937 926 829 750 727 490 277 219 195 175 135 121 42 40 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 21 21 21 20 18 17 15 15 11 11 4 HincII GTy'rAC Cuts at: 669 1657 1938 2668 3454 5566 6592 8275 12117 Size: 988 281 730 786 2112 1026 1683 3842 Cuts at: 12117 12175 14624 14970 15216 669 Size: 58 2449 346 246 3079 Fragments arranged by size: 3842 3079 2449 2112 1683 1026 988 786 730 346 281 246 58 HindIII A'AGCT_T Cuts at: 748 5790 6371 10266 748 Size: 5042 581 3895 8108 Fragments arranged by size: 8108 5042 3895 581 HpaI GTT'AAC Cuts at: 8275 12175 15216 8275 Size: 3900 3041 10685 Fragments arranged by size: 10685 3900 3041 KpnI G_GTAC'C Cuts at: 14951 14951 Size: 17626 MfeI C'AATT_G Cuts at: 2616 5721 15225 2616 Size: 3105 9504 5017 Fragments arranged by size: 9504 5017 3105 MluI A'CGCG_T Cuts at: 17502 17502 Size: 17626 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2196 2217 3320 3459 5800 5890 9968 10960 Size: 891 21 1103 139 2341 90 4078 992 Cuts at: 10960 12601 12610 12775 13637 16740 16924 1305 Size: 1641 9 165 862 3103 184 2007 Fragments arranged by size: 4078 3103 2341 2007 1641 1103 992 891 862 184 165 139 90 21 9 MscI TGG'CCA Cuts at: 10 64 1489 1832 5217 5438 10 Size: 54 1425 343 3385 221 12198 Fragments arranged by size: 12198 3385 1425 343 221 54 MslI CAynn'nnrTG Cuts at: 518 1166 2101 3558 4830 7043 7163 8541 8958 Size: 648 935 1457 1272 2213 120 1378 417 Cuts at: 8958 10017 15584 15943 16102 518 Size: 1059 5567 359 159 2042 Fragments arranged by size: 5567 2213 2042 1457 1378 1272 1059 935 648 417 359 159 120 MspA1I CmG'CkG Cuts at: 1606 4559 7076 9227 11261 11702 14084 15042 15719 Size: 2953 2517 2151 2034 441 2382 958 677 Cuts at: 15719 16867 17112 1606 Size: 1148 245 2120 Fragments arranged by size: 2953 2517 2382 2151 2120 2034 1148 958 677 441 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2942 8834 13071 13266 513 Size: 552 939 938 5892 4237 195 4873 Fragments arranged by size: 5892 4873 4237 939 938 552 195 NdeI CA'TA_TG Cuts at: 387 3071 8725 9589 10597 12663 13492 387 Size: 2684 5654 864 1008 2066 829 4521 Fragments arranged by size: 5654 4521 2684 2066 1008 864 829 NheI G'CTAG_C Cuts at: 1052 12301 1052 Size: 11249 6377 NotI GC'GGCC_GC Cuts at: 15073 15073 Size: 17626 NsiI A_TGCA'T Cuts at: 3415 4233 4237 10020 12744 14776 3415 Size: 818 4 5783 2724 2032 6265 Fragments arranged by size: 6265 5783 2724 2032 818 4 NspI r_CATG'y Cuts at: 1132 1229 4231 4235 6924 8809 10018 10094 10637 Size: 97 3002 4 2689 1885 1209 76 543 Cuts at: 10637 11029 12550 12742 12805 13089 13626 14778 14984 Size: 392 1521 192 63 284 537 1152 206 Cuts at: 14984 16529 1132 Size: 1545 2229 Fragments arranged by size: 3002 2689 2229 1885 1545 1521 1209 1152 543 537 392 284 206 192 97 76 63 4 PacI TTA_AT'TAA Cuts at: 11532 11532 Size: 17626 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 17626 PciI A'CATG_T Cuts at: 6920 10090 10633 12546 12801 16525 6920 Size: 3170 543 1913 255 3724 8021 Fragments arranged by size: 8021 3724 3170 1913 543 255 PflMI CCAn_nnn'nTGG Cuts at: 1108 3428 4891 7921 11311 1108 Size: 2320 1463 3030 3390 7423 Fragments arranged by size: 7423 3390 3030 2320 1463 PfoI T'CCnGG_A Cuts at: 6777 7783 10210 6777 Size: 1006 2427 14193 Fragments arranged by size: 14193 2427 1006 PmeI GTTT'AAAC Cuts at: 14930 14930 Size: 17626 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 15695 15727 17208 17240 15695 Size: 32 1481 32 16081 Fragments arranged by size: 16081 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 17626 PsiI TTA'TAA Cuts at: 2634 3876 5958 12960 15196 2634 Size: 1242 2082 7002 2236 5064 Fragments arranged by size: 7002 5064 2236 2082 1242 PspXI vC'TCGA_Gb Cuts at: 3081 3081 Size: 17626 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 9336 9368 11991 12023 9336 Size: 32 2623 32 14939 Fragments arranged by size: 14939 2623 32 32 PstI C_TGCA'G Cuts at: 830 6024 8991 11260 11521 13438 13931 14978 830 Size: 5194 2967 2269 261 1917 493 1047 3478 Fragments arranged by size: 5194 3478 2967 2269 1917 1047 493 261 PvuI CG_AT'CG Cuts at: 2001 14203 2001 Size: 12202 5424 PvuII CAG'CTG Cuts at: 4559 7076 9227 11261 11702 14084 4559 Size: 2517 2151 2034 441 2382 8101 Fragments arranged by size: 8101 2517 2382 2151 2034 441 SacI G_AGCT'C Cuts at: 9739 11617 12595 13588 9739 Size: 1878 978 993 13777 Fragments arranged by size: 13777 1878 993 978 SalI G'TCGA_C Cuts at: 1936 14622 14968 1936 Size: 12686 346 4594 Fragments arranged by size: 12686 4594 346 SapI GCTCTTCn'nnn_ Cuts at: 2042 5328 14496 14556 2042 Size: 3286 9168 60 5112 Fragments arranged by size: 9168 5112 3286 60 SbfI CC_TGCA'GG Cuts at: 14978 14978 Size: 17626 ScaI AGT'ACT Cuts at: 9022 9121 11254 15874 17586 9022 Size: 99 2133 4620 1712 9062 Fragments arranged by size: 9062 4620 2133 1712 99 SexAI A'CCwGG_T Cuts at: 13257 13257 Size: 17626 SfcI C'TryA_G Cuts at: 826 1045 4197 6020 8946 8987 9291 11256 11517 Size: 219 3152 1823 2926 41 304 1965 261 Cuts at: 11517 11865 13434 13469 13927 14974 16109 16790 16981 Size: 348 1569 35 458 1047 1135 681 191 Cuts at: 16981 826 Size: 1471 Fragments arranged by size: 3152 2926 1965 1823 1569 1471 1135 1047 681 458 348 304 261 219 191 41 35 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 17626 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 17626 SmaI CCC'GGG Cuts at: 1872 14953 1872 Size: 13081 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3081 6579 7032 8139 8732 9824 Size: 197 926 1138 3498 453 1107 593 1092 Cuts at: 9824 10139 10256 11477 11708 12042 12345 15730 16631 Size: 315 117 1221 231 334 303 3385 901 Cuts at: 16631 16893 820 Size: 262 1553 Fragments arranged by size: 3498 3385 1553 1221 1138 1107 1092 926 901 593 453 334 315 303 262 231 197 117 SnaBI TAC'GTA Cuts at: 493 15417 493 Size: 14924 2702 SpeI A'CTAG_T Cuts at: 152 5672 152 Size: 5520 12106 SphI G_CATG'C Cuts at: 1132 4235 11029 12742 14984 1132 Size: 3103 6794 1713 2242 3774 Fragments arranged by size: 6794 3774 3103 2242 1713 SspI AAT'ATT Cuts at: 5 52 2960 4129 4400 4732 6324 6823 7185 Size: 47 2908 1169 271 332 1592 499 362 Cuts at: 7185 9202 11167 11293 11817 12355 14854 15550 5 Size: 2017 1965 126 524 538 2499 696 2081 Fragments arranged by size: 2908 2499 2081 2017 1965 1592 1169 696 538 524 499 362 332 271 126 47 StuI AGG'CCT Cuts at: 1517 11775 1517 Size: 10258 7368 StyI C'CwwG_G Cuts at: 513 1065 2004 2942 5653 5663 8834 13071 13266 Size: 552 939 938 2711 10 3171 4237 195 Cuts at: 13266 15065 513 Size: 1799 3074 Fragments arranged by size: 4237 3171 3074 2711 1799 939 938 552 195 10 SwaI ATTT'AAAT Cuts at: 5754 6030 5754 Size: 276 17350 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2875 4442 6040 11086 11269 15653 15670 Size: 1255 1295 1567 1598 5046 183 4384 17 Cuts at: 15670 15823 16008 16453 325 Size: 153 185 445 1498 Fragments arranged by size: 5046 4384 1598 1567 1498 1295 1255 445 185 183 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4263 4639 7252 Size: 274 80 33 51 1443 2286 376 2613 Cuts at: 7252 9020 9072 9119 10156 10945 11252 11738 12510 Size: 1768 52 47 1037 789 307 486 772 Cuts at: 12510 13036 13741 13756 14263 14335 15872 17584 96 Size: 526 705 15 507 72 1537 1712 138 Fragments arranged by size: 2613 2286 1768 1712 1537 1443 1037 789 772 705 526 507 486 376 307 274 138 80 72 52 51 47 33 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2493 2525 4197 4229 7363 7395 8149 8181 14881 Size: 32 1672 32 3134 32 754 32 6700 Cuts at: 14881 14913 17586 17618 2493 Size: 32 2673 32 2501 Fragments arranged by size: 6700 3134 2673 2501 1672 754 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 10686 11695 1316 Size: 9370 1009 7247 Fragments arranged by size: 9370 7247 1009 XbaI T'CTAG_A Cuts at: 5677 8327 8818 9193 12325 14962 5677 Size: 2650 491 375 3132 2637 8341 Fragments arranged by size: 8341 3132 2650 2637 491 375 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5189 9512 5189 Size: 4323 13303 XhoI C'TCGA_G Cuts at: 1943 3081 1943 Size: 1138 16488 XmaI C'CCGG_G Cuts at: 1870 14951 1870 Size: 13081 4545 XmnI GAAnn'nnTTC Cuts at: 2036 2063 4418 4698 5412 7488 8253 9201 9348 Size: 27 2355 280 714 2076 765 948 147 Cuts at: 9348 10070 11111 11167 11199 11319 11445 12006 12321 Size: 722 1041 56 32 120 126 561 315 Cuts at: 12321 12753 15755 2036 Size: 432 3002 3907 Fragments arranged by size: 3907 3002 2355 2076 1041 948 765 722 714 561 432 315 280 147 126 120 56 32 27 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 15434 276 Size: 53 83 186 936 13900 2468 Fragments arranged by size: 13900 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI BbeI BsiWI FseI FspAI KasI NaeI NarI NgoMIV NruI PmlI PpuMI PspOMI RsrII SacII SanDI SfiI SfoI SrfI