Restriction map for
pFN21AA1995
(Circular) (Six-base) MAPSORT of: pFN21AA1995.seq Check: 1813 from: 1 to: 7642
pFN21AA1995 7642 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4681 4810 4681
Size: 129 7513
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5452 278
Size: 53 83 186 936 3916 2468
Fragments arranged by size:
3916 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4930 4985 1937
Size: 2993 55 4594
Fragments arranged by size:
4594 2993 55
Acc65I G'GTAC_C
Cuts at: 2469 4963 2469
Size: 2494 5148
AclI AA'CG_TT
Cuts at: 1563 5769 6142 1563
Size: 4206 373 3063
Fragments arranged by size:
4206 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3030 3423 4590 5705 7089 7357 1632
Size: 1398 393 1167 1115 1384 268 1917
Fragments arranged by size:
1917 1398 1384 1167 1115 393 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7445
AflIII A'CryG_T
Cuts at: 1203 2655 6541 7518 1203
Size: 1452 3886 977 1327
Fragments arranged by size:
3886 1452 1327 977
AgeI A'CCGG_T
Cuts at: 6473 6473
Size: 7642
AhdI GACnn_n'nnGTC
Cuts at: 6371 6371
Size: 7642
AleI CACnn'nnGTG
Cuts at: 1166 3182 1166
Size: 2016 5626
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2515 2734 3144 4703 1184
Size: 1331 219 410 1559 4123
Fragments arranged by size:
4123 1559 1331 410 219
AlwNI CAG_nnn'CTG
Cuts at: 3088 4425 4517 4717 5385 6957 3088
Size: 1337 92 200 668 1572 3773
Fragments arranged by size:
3773 1572 1337 668 200 92
ApaLI G'TGCA_C
Cuts at: 5699 6855 5699
Size: 1156 6486
ApoI r'AATT_y
Cuts at: 1493 2802 3683 4189 4463 4951 5177 5329 7438
Size: 1309 881 506 274 488 226 152 2109
Cuts at: 7438 1493
Size: 1697
Fragments arranged by size:
2109 1697 1309 881 506 488 274 226
152
AseI AT'TA_AT
Cuts at: 160 1032 6196 160
Size: 872 5164 1606
Fragments arranged by size:
5164 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7642
AvaI C'yCGr_G
Cuts at: 1870 1943 2086 4255 4967 1870
Size: 73 143 2169 712 4545
Fragments arranged by size:
4545 2169 712 143 73
BamHI G'GATC_C
Cuts at: 2878 4873 4972 5339 2878
Size: 1995 99 367 5181
Fragments arranged by size:
5181 1995 367 99
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2186 2469 4905 4963 6418
Size: 325 217 627 399 283 2436 58 1455
Cuts at: 6418 618
Size: 1842
Fragments arranged by size:
2436 1842 1455 627 399 325 283 217
58
BanII G_rGCy'C
Cuts at: 1391 2670 4250 4961 1391
Size: 1279 1580 711 4072
Fragments arranged by size:
4072 1580 1279 711
BbeI G_GCGC'C
Cuts at: 2190 2190
Size: 7642
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6671
BbvCI CC'TCA_GC
Cuts at: 3437 3437
Size: 7642
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2786 2820 5833 5867 1903
Size: 34 849 34 3013 34 3678
Fragments arranged by size:
3678 3013 849 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5534 6744 5534
Size: 1210 6432
BclI T'GATC_A
Cuts at: 1548 3960 1548
Size: 2412 5230
BfrBI ATG'CAT
Cuts at: 4831 4831
Size: 7642
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6253 136
Size: 107 122 71 631 605 4581 1525
Fragments arranged by size:
4581 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7637 7637
Size: 7642
BlpI GC'TnA_GC
Cuts at: 5059 5059
Size: 7642
Bme1580I G_kGCm'C
Cuts at: 1790 3118 5703 6859 1790
Size: 1328 2585 1156 2573
Fragments arranged by size:
2585 2573 1328 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7642
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3548 3643 5373
Size: 416 53 83 399 24 2131 95 1730
Cuts at: 5373 6331 442
Size: 958 1753
Fragments arranged by size:
2131 1753 1730 958 416 399 95 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7642
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2245 3475 2245
Size: 1230 6412
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2646 4751 6302 1239
Size: 1407 2105 1551 2579
Fragments arranged by size:
2579 2105 1551 1407
Bpu10I CC'TnA_GC
Cuts at: 2883 3437 2883
Size: 554 7088
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5767 6632 6930 5767
Size: 865 298 6479
Fragments arranged by size:
6479 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2884 2980 3463 3973 4063 6305 882
Size: 617 1385 96 483 510 90 2242 2219
Fragments arranged by size:
2242 2219 1385 617 510 483 96 90
BsaAI yAC'GTr
Cuts at: 493 1204 5433 493
Size: 711 4229 2702
Fragments arranged by size:
4229 2702 711
BsaBI GATnn'nnATC
Cuts at: 4008 7636 4008
Size: 3628 4014
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2187 5449 5831 6466
Size: 53 83 186 936 654 3262 382 635
Cuts at: 6466 275
Size: 1451
Fragments arranged by size:
3262 1451 936 654 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6075 6473 6747 6894 7305 6075
Size: 398 274 147 411 6412
Fragments arranged by size:
6412 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3224 3254 1178
Size: 30 165 30 1821 30 5566
Fragments arranged by size:
5566 1821 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4275 4482 4653 1186
Size: 3089 207 171 4175
Fragments arranged by size:
4175 3089 207 171
BseYI C'CCAG_C
Cuts at: 2014 3220 3694 3889 4640 4934 6845 7341 2014
Size: 1206 474 195 751 294 1911 496 2315
Fragments arranged by size:
2315 1911 1206 751 496 474 294 195
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2138 3150 4521 4699 1163
Size: 573 402 1012 1371 178 4106
Fragments arranged by size:
4106 1371 1012 573 402 178
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5092 5853 6881 1540
Size: 275 186 3091 761 1028 2301
Fragments arranged by size:
3091 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2670 4250 5703 5788 6859 2670
Size: 1580 1453 85 1071 3453
Fragments arranged by size:
3453 1580 1453 1071 85
BsmI GAATG_Cn'
Cuts at: 4831 5153 5246 4831
Size: 322 93 7227
Fragments arranged by size:
7227 322 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2624 2624
Size: 7642
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2670 3118 4250 4961 5703 5788 6859
Size: 399 880 448 1132 711 742 85 1071
Cuts at: 6859 1391
Size: 2174
Fragments arranged by size:
2174 1132 1071 880 742 711 448 399
85
BspEI T'CCGG_A
Cuts at: 7305 7305
Size: 7642
BspHI T'CATG_A
Cuts at: 5531 7261 5531
Size: 1730 5912
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3112 3445 4681 4810 4997 844
Size: 2268 333 1236 129 187 3489
Fragments arranged by size:
3489 2268 1236 333 187 129
BsrBI CCG'CTC
Cuts at: 1394 1823 5529 1394
Size: 429 3706 3507
Fragments arranged by size:
3706 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3018 3807 3990 4036 4059 6137 6311
Size: 1183 1770 789 183 46 23 2078 174
Cuts at: 6311 65
Size: 1396
Fragments arranged by size:
2078 1770 1396 1183 789 183 174 46
23
BsrFI r'CCGG_y
Cuts at: 1700 1810 2123 3852 6286 6473 1700
Size: 110 313 1729 2434 187 2869
Fragments arranged by size:
2869 2434 1729 313 187 110
BsrGI T'GTAC_A
Cuts at: 96 3174 96
Size: 3078 4564
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7642
BssSI C'ACGA_G
Cuts at: 5702 6714 5702
Size: 1012 6630
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3020 794
Size: 2226 5416
BstBI TT'CG_AA
Cuts at: 4441 6446 4441
Size: 2005 5637
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7642
BstXI CCAn_nnnn'nTGG
Cuts at: 2513 2513
Size: 7642
BstYI r'GATC_y
Cuts at: 1972 2878 3798 4118 4873 4972 5339 5724 5741
Size: 906 920 320 755 99 367 385 17
Cuts at: 5741 7193 7279 7291 7637 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 920 906 755 385 367 346
320 99 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2182 2622 2811 3211 3226 4150 4395
Size: 552 1117 440 189 400 15 924 245
Cuts at: 4395 513
Size: 3760
Fragments arranged by size:
3760 1117 924 552 440 400 245 189
15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6903
Fragments arranged by size:
6903 683 56
BtsI GCAGTG_nn'
Cuts at: 2032 2315 2499 4222 5158 5952 5972 2032
Size: 283 184 1723 936 794 20 3702
Fragments arranged by size:
3702 1723 936 794 283 184 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7642
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3243 3278 3862 3897 4198 4233 529
Size: 35 2679 35 584 35 301 35 3938
Fragments arranged by size:
3938 2679 584 301 35 35 35 35
DraI TTT'AAA
Cuts at: 4946 4946
Size: 7642
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2266 6649 809
Size: 507 950 4383 1802
Fragments arranged by size:
4383 1802 950 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3774 4042 5089
Size: 54 1425 50 275 18 1944 268 1047
Cuts at: 5089 5978 7607 8
Size: 889 1629 43
Fragments arranged by size:
1944 1629 1425 1047 889 275 268 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5089 1537
Size: 275 3277 4090
Fragments arranged by size:
4090 3277 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2448 2928 3549 4284 4536 4854 5572 1353
Size: 1095 480 621 735 252 318 718 3423
Fragments arranged by size:
3423 1095 735 718 621 480 318 252
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2153 6229 6603 6749 387
Size: 1064 138 564 4076 374 146 1280
Fragments arranged by size:
4076 1280 1064 564 374 146 138
EcoICRI GAG'CTC
Cuts at: 2668 4248 2668
Size: 1580 6062
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2646 3030 3423 4590 4751 5705 6302
Size: 393 1014 384 393 1167 161 954 597
Cuts at: 6302 7089 7357 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1167 1014 954 787 597 393 393
384 268 161
EcoNI CCTnn'n_nnAGG
Cuts at: 2746 2873 2746
Size: 127 7515
EcoO109I rG'GnC_Cy
Cuts at: 2096 3938 2096
Size: 1842 5800
EcoRI G'AATT_C
Cuts at: 2802 3683 4189 4951 5329 2802
Size: 881 506 762 378 5115
Fragments arranged by size:
5115 881 762 506 378
EcoRV GAT'ATC
Cuts at: 2704 4087 2704
Size: 1383 6259
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4903 4903
Size: 7642
FspI TGC'GCA
Cuts at: 6148 6148
Size: 7642
HaeII r_GCGC'y
Cuts at: 1678 2106 2190 6789 1678
Size: 428 84 4599 2531
Fragments arranged by size:
4599 2531 428 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3224 3256 4472 4504 4923
Size: 32 135 32 1652 32 1216 32 419
Cuts at: 4923 4955 6283 6315 6357 6389 1373
Size: 32 1328 32 42 32 2626
Fragments arranged by size:
2626 1652 1328 1216 419 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2821 3645 4986 5232 669
Size: 988 281 883 824 1341 246 3079
Fragments arranged by size:
3079 1341 988 883 824 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7642
HpaI GTT'AAC
Cuts at: 5232 5232
Size: 7642
KasI G'GCGC_C
Cuts at: 2186 2186
Size: 7642
KpnI G_GTAC'C
Cuts at: 2473 4967 2473
Size: 2494 5148
MfeI C'AATT_G
Cuts at: 5241 5241
Size: 7642
MluI A'CGCG_T
Cuts at: 7518 7518
Size: 7642
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6756 6940 1305
Size: 5451 184 2007
Fragments arranged by size:
5451 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3776 10
Size: 54 1425 343 1944 3876
Fragments arranged by size:
3876 1944 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3182 3380 5600 5959 6118 518
Size: 648 843 1173 198 2220 359 159 2042
Fragments arranged by size:
2220 2042 1173 843 648 359 198 159
MspA1I CmG'CkG
Cuts at: 1606 2184 3220 3424 3833 3889 5058 5735 6883
Size: 578 1036 204 409 56 1169 677 1148
Cuts at: 6883 7128 1606
Size: 245 2120
Fragments arranged by size:
2120 1169 1148 1036 677 578 409 245
204 56
NaeI GCC'GGC
Cuts at: 2125 3854 2125
Size: 1729 5913
NarI GG'CG_CC
Cuts at: 2187 2187
Size: 7642
NcoI C'CATG_G
Cuts at: 513 1065 2622 2811 3211 3226 4395 513
Size: 552 1557 189 400 15 1169 3760
Fragments arranged by size:
3760 1557 1169 552 400 189 15
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7642
NgoMIV G'CCGG_C
Cuts at: 2123 3852 2123
Size: 1729 5913
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7642
NotI GC'GGCC_GC
Cuts at: 5089 5089
Size: 7642
NsiI A_TGCA'T
Cuts at: 4833 4833
Size: 7642
NspI r_CATG'y
Cuts at: 1132 1229 2659 3193 3263 3715 5000 6545 1132
Size: 97 1430 534 70 452 1285 1545 2229
Fragments arranged by size:
2229 1545 1430 1285 534 452 97 70
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7642
PciI A'CATG_T
Cuts at: 2655 6541 2655
Size: 3886 3756
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3088 1108
Size: 1980 5662
PfoI T'CCnGG_A
Cuts at: 4134 4134
Size: 7642
PmeI GTTT'AAAC
Cuts at: 4946 4946
Size: 7642
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5711 5743 7224 7256 5711
Size: 32 1481 32 6097
Fragments arranged by size:
6097 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3938 3938
Size: 7642
PshAI GACnn'nnGTC
Cuts at: 1613 2088 2992 1613
Size: 475 904 6263
Fragments arranged by size:
6263 904 475
PsiI TTA'TAA
Cuts at: 3795 5212 3795
Size: 1417 6225
PspXI vC'TCGA_Gb
Cuts at: 2086 2086
Size: 7642
PstI C_TGCA'G
Cuts at: 830 2325 2902 4630 4692 4788 4994 830
Size: 1495 577 1728 62 96 206 3478
Fragments arranged by size:
3478 1728 1495 577 206 96 62
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7642
PvuII CAG'CTG
Cuts at: 3220 3424 3833 3889 3220
Size: 204 409 56 6973
Fragments arranged by size:
6973 409 204 56
SacI G_AGCT'C
Cuts at: 2670 4250 2670
Size: 1580 6062
SacII CC_GC'GG
Cuts at: 2185 2185
Size: 7642
SalI G'TCGA_C
Cuts at: 1936 4984 1936
Size: 3048 4594
SanDI GG'GwC_CC
Cuts at: 3938 3938
Size: 7642
SapI GCTCTTCn'nnn_
Cuts at: 4536 4536
Size: 7642
SbfI CC_TGCA'GG
Cuts at: 4994 4994
Size: 7642
ScaI AGT'ACT
Cuts at: 2834 5890 7602 2834
Size: 3056 1712 2874
Fragments arranged by size:
3056 2874 1712
SfcI C'TryA_G
Cuts at: 826 1045 2321 2898 3024 3109 4626 4688 4751
Size: 219 1276 577 126 85 1517 62 63
Cuts at: 4751 4784 4927 4990 6125 6806 6997 826
Size: 33 143 63 1135 681 191 1471
Fragments arranged by size:
1517 1471 1276 1135 681 577 219 191
143 126 85 63 63 62 33
SfoI GGC'GCC
Cuts at: 2188 2188
Size: 7642
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7642
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7642
SmaI CCC'GGG
Cuts at: 1872 4257 4969 1872
Size: 2385 712 4545
Fragments arranged by size:
4545 2385 712
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2086 5746 6647 6909 820
Size: 197 926 143 3660 901 262 1553
Fragments arranged by size:
3660 1553 926 901 262 197 143
SnaBI TAC'GTA
Cuts at: 493 5433 493
Size: 4940 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7642
SphI G_CATG'C
Cuts at: 1132 5000 1132
Size: 3868 3774
SspI AAT'ATT
Cuts at: 5 52 2393 2548 5566 5
Size: 47 2341 155 3018 2081
Fragments arranged by size:
3018 2341 2081 155 47
StuI AGG'CCT
Cuts at: 1517 3596 1517
Size: 2079 5563
StyI C'CwwG_G
Cuts at: 513 1065 2559 2622 2811 2990 3211 3226 3591
Size: 552 1494 63 189 179 221 15 365
Cuts at: 3591 3771 3943 4395 5081 513
Size: 180 172 452 686 3074
Fragments arranged by size:
3074 1494 686 552 452 365 221 189
180 179 172 63 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3031 3172 4374 5669 5686 5839 6024
Size: 1255 1451 141 1202 1295 17 153 185
Cuts at: 6024 6469 325
Size: 445 1498
Fragments arranged by size:
1498 1451 1295 1255 1202 445 185 153
141 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2685 2832 3174
Size: 274 80 33 51 1443 708 147 342
Cuts at: 3174 5888 7600 96
Size: 2714 1712 138
Fragments arranged by size:
2714 1712 1443 708 342 274 147 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2024 2056 4545 4577 7602 7634 2024
Size: 32 2489 32 3025 32 2032
Fragments arranged by size:
3025 2489 2032 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3649 1316
Size: 2333 5309
XbaI T'CTAG_A
Cuts at: 2911 4978 2911
Size: 2067 5575
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2173 4393 2011
Size: 162 2220 5260
Fragments arranged by size:
5260 2220 162
XhoI C'TCGA_G
Cuts at: 1943 2086 1943
Size: 143 7499
XmaI C'CCGG_G
Cuts at: 1870 4255 4967 1870
Size: 2385 712 4545
Fragments arranged by size:
4545 2385 712
XmnI GAAnn'nnTTC
Cuts at: 3431 5771 3431
Size: 2340 5302
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5450 276
Size: 53 83 186 936 3916 2468
Fragments arranged by size:
3916 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BsiWI BstZ17I
Bsu36I DraIII FseI FspAI NruI PacI PmlI PspOMI
PsrI RsrII SexAI SfiI SrfI SwaI