Restriction map for
pFN21AA1944
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# NO CUT ENZYMES ( 76 enzymes) #
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Acc65I AclI AflII AflIII AgeI
Aor51HI AscI AseI AsnI Asp718I
AviII AvrII BciVI BclI BfaI
BfrI BglI BlnI BsaBI BsiWI
BsmI Bsp1407I BspLU11I BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I CpoI
DraI DraIII Eco47III EcoRI EcoT22I
FbaI FspI HindIII HpaI KpnI
MaeI MamI MfeI MluI MunI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XbaI
XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 169 GACGTC GACGT^C
AccI 412 GTCTAC GT^[AC][GT]AC
AccIII 749 TCCGGA T^CCGGA
AhdI 81 GACGGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 688 GTGCAC G^TGCAC
AocI 501 CCTGAGG CC^T[ACGT]AGG
ApaI 781 GGGCCC GGGCC^C
ApaLI 688 GTGCAC G^TGCAC
Asp700I 2184 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 81 GACGGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 1123 GGCGCC GGCGC^C
BbrPI 1197 CACGTG CAC^GTG
BcgI 2981 GCATGGATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1250 AGATCT A^GATCT
BsaAI 1197 CACGTG [CT]AC^GT[GA]
BseAI 749 TCCGGA T^CCGGA
BspEI 749 TCCGGA T^CCGGA
BsrBI 1684 GAGCGG CCGCTC(-3/-3)
BstAPI 309 GCAAGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 501 CCTGAGG CC^T[ACGT]AGG
EagI 1760 CGGCCG C^GGCCG
Eam1105I 81 GACGGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1760 CGGCCG C^GGCCG
Eco52I 1760 CGGCCG C^GGCCG
Eco81I 501 CCTGAGG CC^T[ACGT]AGG
EcoRV 202 GATATC GAT^ATC
FseI 1761 GGCCGGCC GGCCGG^CC
HincII 1498 GTCAAC GT[CT]^[GA]AC
HindII 1498 GTCAAC GT[CT]^[GA]AC
KasI 1123 GGCGCC G^GCGCC
KspI 1695 CCGCGG CCGC^GG
MroI 749 TCCGGA T^CCGGA
NarI 1123 GGCGCC GG^CGCC
NcoI 1724 CCATGG C^CATGG
PmaCI 1197 CACGTG CAC^GTG
PmlI 1197 CACGTG CAC^GTG
SacII 1695 CCGCGG CCGC^GG
SapI 1162 GAAGAGC GCTCTTC(1/4)
ScaI 2519 AGTACT AGT^ACT
SfoI 1123 GGCGCC GGC^GCC
SmaI 230 CCCGGG CCC^GGG
SmlI 100 CTTGAG C^T[CT][GA]AG
StuI 1648 AGGCCT AGG^CCT
XmaCI 230 CCCGGG C^CCGGG
XmaI 230 CCCGGG C^CCGGG
XmnI 2184 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcyI 169 GACGTC G[GA]^CG[CT]C
1123 GGCGCC
BamHI 95 GGATCC G^GATCC
746 GGATCC
BbvCI 3009 CCTCAGC CCTCAGC(-5/-2)
1753 GCTGAGG
BlpI 2043 GCTCAGC GC^T[ACGT]AGC
2736 GCTGAGC
Bpu1102I 2043 GCTCAGC GC^T[ACGT]AGC
2736 GCTGAGC
BsaHI 169 GACGTC G[GA]^CG[CT]C
1123 GGCGCC
BsaWI 749 TCCGGA [AT]^CCGG[AT]
2906 ACCGGA
BsiEI 471 CGACCG CG[GA][CT]^CG
1760 CGGCCG
BspDI 769 ATCGAT AT^CGAT
1147 ATCGAT
BspHI 206 TCATGA T^CATGA
2883 TCATGA
BstEII 1171 GGTGACC G^GT[ACGT]ACC
1986 GGTGACC
BstPI 1171 GGTGACC G^GT[ACGT]ACC
1986 GGTGACC
CelII 2043 GCTCAGC GC^T[ACGT]AGC
2736 GCTGAGC
ClaI 769 ATCGAT AT^CGAT
1147 ATCGAT
DrdI 2019 GACAGGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2568 GACAGACAGGTC
EcoO65I 1171 GGTGACC G^GT[ACGT]ACC
1986 GGTGACC
EcoT14I 1724 CCATGG C^C[AT][AT]GG
2165 CCTTGG
HaeII 478 AGCGCC [GA]GCGC^[CT]
1123 GGCGCC
Hin1I 169 GACGTC G[GA]^CG[CT]C
1123 GGCGCC
RcaI 206 TCATGA T^CATGA
2883 TCATGA
StyI 1724 CCATGG C^C[AT][AT]GG
2165 CCTTGG
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# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
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AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AspI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII