Restriction map for
pFN21AA1944
(Circular) (Six-base) MAPSORT of: pFN21AA1944.seq Check: 4415 from: 1 to: 8002
pFN21AA1944 8002 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3801 3838 3801
Size: 37 7965
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2178 5812 278
Size: 53 83 186 936 642 3634 2468
Fragments arranged by size:
3634 2468 936 642 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2418 5345 1937
Size: 481 2927 4594
Fragments arranged by size:
4594 2927 481
Acc65I G'GTAC_C
Cuts at: 5323 5323
Size: 8002
AclI AA'CG_TT
Cuts at: 1563 6129 6502 1563
Size: 4566 373 3063
Fragments arranged by size:
4566 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2210 2973 6065 7449 7717 1632
Size: 578 763 3092 1384 268 1917
Fragments arranged by size:
3092 1917 1384 763 578 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7805
AflIII A'CryG_T
Cuts at: 1203 6901 7878 1203
Size: 5698 977 1327
Fragments arranged by size:
5698 1327 977
AgeI A'CCGG_T
Cuts at: 6833 6833
Size: 8002
AhdI GACnn_n'nnGTC
Cuts at: 2091 6731 2091
Size: 4640 3362
AleI CACnn'nnGTG
Cuts at: 1166 4085 1166
Size: 2919 5083
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4986 5282 1184
Size: 3802 296 3904
Fragments arranged by size:
3904 3802 296
AlwNI CAG_nnn'CTG
Cuts at: 2446 2469 3535 3892 3931 4137 5242 5745 7317
Size: 23 1066 357 39 206 1105 503 1572
Cuts at: 7317 2446
Size: 3131
Fragments arranged by size:
3131 1572 1105 1066 503 357 206 39
23
ApaI G_GGCC'C
Cuts at: 2790 2790
Size: 8002
ApaLI G'TGCA_C
Cuts at: 2693 6059 7215 2693
Size: 3366 1156 3480
Fragments arranged by size:
3480 3366 1156
ApoI r'AATT_y
Cuts at: 1493 2369 2944 4253 4391 5141 5311 5537 5689
Size: 876 575 1309 138 750 170 226 152
Cuts at: 5689 7798 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1309 876 750 575 226 170
152 138
AseI AT'TA_AT
Cuts at: 160 1032 6556 160
Size: 872 5524 1606
Fragments arranged by size:
5524 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8002
AvaI C'yCGr_G
Cuts at: 1870 1943 2235 2532 4007 5327 1870
Size: 73 292 297 1475 1320 4545
Fragments arranged by size:
4545 1475 1320 297 292 73
BamHI G'GATC_C
Cuts at: 2100 2751 5332 5699 2100
Size: 651 2581 367 4403
Fragments arranged by size:
4403 2581 651 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2034 2320 3128 3565 4295
Size: 325 217 627 247 286 808 437 730
Cuts at: 4295 4565 5323 6778 618
Size: 270 758 1455 1842
Fragments arranged by size:
1842 1455 808 758 730 627 437 325
286 270 247 217
BanII G_rGCy'C
Cuts at: 1391 2598 2682 2790 3269 3609 3803 3861 5106
Size: 1207 84 108 479 340 194 58 1245
Cuts at: 5106 5321 1391
Size: 215 4072
Fragments arranged by size:
4072 1245 1207 479 340 215 194 108
84 58
BbeI G_GCGC'C
Cuts at: 3132 3132
Size: 8002
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2736 3347 3441 4774 4795 928
Size: 971 837 611 94 1333 21 4135
Fragments arranged by size:
4135 1333 971 837 611 94 21
BbvCI CC'TCA_GC
Cuts at: 3759 5015 3759
Size: 1256 6746
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4975 5009 6193 6227 1903
Size: 34 3038 34 1184 34 3678
Fragments arranged by size:
3678 3038 1184 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5894 7104 5894
Size: 1210 6792
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8002
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6613 136
Size: 107 122 71 631 605 4941 1525
Fragments arranged by size:
4941 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3255 7997 3255
Size: 4742 3260
BlpI GC'TnA_GC
Cuts at: 4049 4742 5419 4049
Size: 693 677 6632
Fragments arranged by size:
6632 693 677
Bme1580I G_kGCm'C
Cuts at: 1790 2012 2325 2697 2790 3570 3636 4568 6063
Size: 222 313 372 93 780 66 932 1495
Cuts at: 6063 7219 1790
Size: 1156 2573
Fragments arranged by size:
2573 1495 1156 932 780 372 313 222
93 66
BmgBI CAC'GTC
Cuts at: 1318 2914 3234 3439 1318
Size: 1596 320 205 5881
Fragments arranged by size:
5881 1596 320 205
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2479 4903 5733
Size: 416 53 83 399 24 1062 2424 830
Cuts at: 5733 6691 442
Size: 958 1753
Fragments arranged by size:
2424 1753 1062 958 830 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8002
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4902 4902
Size: 8002
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2206 2612 2923 3425 3569 3628 6662 1239
Size: 967 406 311 502 144 59 3034 2579
Fragments arranged by size:
3034 2579 967 502 406 311 144 59
Bpu10I CC'TnA_GC
Cuts at: 2423 3536 3759 4092 5015 2423
Size: 1113 223 333 923 5410
Fragments arranged by size:
5410 1113 923 333 223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2126 6127 6992 7290 2126
Size: 4001 865 298 2838
Fragments arranged by size:
4001 2838 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2522 3373 3598 6665 882
Size: 617 1023 851 225 3067 2219
Fragments arranged by size:
3067 2219 1023 851 617 225
BsaAI yAC'GTr
Cuts at: 493 1204 3204 5793 493
Size: 711 2000 2589 2702
Fragments arranged by size:
2702 2589 2000 711
BsaBI GATnn'nnATC
Cuts at: 7996 7996
Size: 8002
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2175 3129 5809 6191
Size: 53 83 186 936 642 954 2680 382
Cuts at: 6191 6826 275
Size: 635 1451
Fragments arranged by size:
2680 1451 954 936 642 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2754 4911 6435 6833 7107 7254 7665 2754
Size: 2157 1524 398 274 147 411 3091
Fragments arranged by size:
3091 2157 1524 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2585 2615 4931 4961 1178
Size: 30 165 30 1182 30 2316 30 4219
Fragments arranged by size:
4219 2316 1182 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2653 2752 3689 3692 3869 3927 4877 4939
Size: 1467 99 937 3 177 58 950 62
Cuts at: 4939 5003 5155 1186
Size: 64 152 4033
Fragments arranged by size:
4033 1467 950 937 177 152 99 64
62 58 3
BseYI C'CCAG_C
Cuts at: 3796 4137 4649 4669 7205 7701 3796
Size: 341 512 20 2536 496 4097
Fragments arranged by size:
4097 2536 512 496 341 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2459 3691 4034 5252 1163
Size: 573 723 1232 343 1218 3913
Fragments arranged by size:
3913 1232 1218 723 573 343
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2479 3768 5452 6213 7241 1540
Size: 275 186 478 1289 1684 761 1028 2301
Fragments arranged by size:
2301 1684 1289 1028 761 478 275 186
BsiHKAI G_wGCw'C
Cuts at: 2598 2682 2697 3609 6063 6148 7219 2598
Size: 84 15 912 2454 85 1071 3381
Fragments arranged by size:
3381 2454 1071 912 85 84 15
BsmI GAATG_Cn'
Cuts at: 5513 5606 5513
Size: 93 7909
BsmBI CGTCTCn'nnnn_
Cuts at: 2182 2904 3646 5167 2182
Size: 722 742 1521 5017
Fragments arranged by size:
5017 1521 742 722
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2325 2598 2682 2697 2790 3269
Size: 399 222 313 273 84 15 93 479
Cuts at: 3269 3570 3609 3636 3803 3861 4568 5106 5321
Size: 301 39 27 167 58 707 538 215
Cuts at: 5321 6063 6148 7219 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 707 538 479 399 313
301 273 222 215 167 93 85 84
58 39 27 15
BspEI T'CCGG_A
Cuts at: 2754 7665 2754
Size: 4911 3091
BspHI T'CATG_A
Cuts at: 2211 4888 5891 7621 2211
Size: 2677 1003 1730 2592
Fragments arranged by size:
2677 2592 1730 1003
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2431 3189 3199 3801 3838 5357 844
Size: 1587 758 10 602 37 1519 3489
Fragments arranged by size:
3489 1587 1519 758 602 37 10
BsrBI CCG'CTC
Cuts at: 1394 1823 3691 5889 1394
Size: 429 1868 2198 3507
Fragments arranged by size:
3507 2198 1868 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2752 4269 4702 6497 6671 65
Size: 1183 1504 1517 433 1795 174 1396
Fragments arranged by size:
1795 1517 1504 1396 1183 433 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2047 2427 2553 3767 6646 6833 1700
Size: 110 237 380 126 1214 2879 187 2869
Fragments arranged by size:
2879 2869 1214 380 237 187 126 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8002
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8002
BssSI C'ACGA_G
Cuts at: 6062 7074 6062
Size: 1012 6990
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2320 794
Size: 1526 6476
BstBI TT'CG_AA
Cuts at: 6806 6806
Size: 8002
BstEII G'GTnAC_C
Cuts at: 1184 3176 3991 1184
Size: 1992 815 5195
Fragments arranged by size:
5195 1992 815
BstXI CCAn_nnnn'nTGG
Cuts at: 3196 4087 4216 3196
Size: 891 129 6982
Fragments arranged by size:
6982 891 129
BstYI r'GATC_y
Cuts at: 1972 2100 2751 3255 3888 3927 5239 5332 5699
Size: 128 651 504 633 39 1312 93 367
Cuts at: 5699 6084 6101 7553 7639 7651 7997 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1312 651 633 504 385 367
346 128 93 86 39 17 12
Bsu36I CC'TnA_GG
Cuts at: 2507 2507
Size: 8002
BtgI C'CryG_G
Cuts at: 513 1065 2622 2649 2673 3372 3700 3729 513
Size: 552 1557 27 24 699 328 29 4786
Fragments arranged by size:
4786 1557 699 552 328 29 27 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7263
Fragments arranged by size:
7263 683 56
BtsI GCAGTG_nn'
Cuts at: 4080 4092 4968 5518 6312 6332 4080
Size: 12 876 550 794 20 5750
Fragments arranged by size:
5750 876 794 550 20 12
ClaI AT'CG_AT
Cuts at: 1554 2775 3153 1554
Size: 1221 378 6403
Fragments arranged by size:
6403 1221 378
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2611 2646 529
Size: 35 2047 35 5885
Fragments arranged by size:
5885 2047 35 35
DraI TTT'AAA
Cuts at: 5306 5306
Size: 8002
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4030 4579 7009 809
Size: 507 2714 549 2430 1802
Fragments arranged by size:
2714 2430 1802 549 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 2583 2874
Size: 54 1425 50 275 18 234 519 291
Cuts at: 2874 3348 3765 4206 4396 4791 5449 6338 7967
Size: 474 417 441 190 395 658 889 1629
Cuts at: 7967 8
Size: 43
Fragments arranged by size:
1629 1425 889 658 519 474 441 417
395 291 275 234 190 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3765 5449 1537
Size: 275 1953 1684 4090
Fragments arranged by size:
4090 1953 1684 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3162 3666 4346 5932 1353
Size: 1809 504 680 1586 3423
Fragments arranged by size:
3423 1809 1586 680 504
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3897 6589 6963 7109 387
Size: 1064 138 2308 2692 374 146 1280
Fragments arranged by size:
2692 2308 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2596 2680 3607 2596
Size: 84 927 6991
Fragments arranged by size:
6991 927 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2206 2210 2612 2923 2973 3425 3569
Size: 393 574 4 402 311 50 452 144
Cuts at: 3569 3628 6065 6662 7449 7717 1239
Size: 59 2437 597 787 268 1524
Fragments arranged by size:
2437 1524 787 597 574 452 402 393
311 268 144 59 50 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2192 2797 4304 4724 5125 2192
Size: 605 1507 420 401 5069
Fragments arranged by size:
5069 1507 605 420 401
EcoO109I rG'GnC_Cy
Cuts at: 2566 2667 2787 3739 5066 2566
Size: 101 120 952 1327 5502
Fragments arranged by size:
5502 1327 952 120 101
EcoRI G'AATT_C
Cuts at: 5311 5689 5311
Size: 378 7624
EcoRV GAT'ATC
Cuts at: 2209 2209
Size: 8002
FseI GG_CCGG'CC
Cuts at: 3771 3771
Size: 8002
FspI TGC'GCA
Cuts at: 6508 6508
Size: 8002
HaeII r_GCGC'y
Cuts at: 1678 2487 3132 7149 1678
Size: 809 645 4017 2531
Fragments arranged by size:
4017 2531 809 645
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2077 2109 2585 2617 3116
Size: 32 135 32 505 32 476 32 499
Cuts at: 3116 3148 3218 3250 3407 3439 3621 3653 3919
Size: 32 70 32 157 32 182 32 266
Cuts at: 3919 3951 5058 5090 6643 6675 6717 6749 1373
Size: 32 1107 32 1553 32 42 32 2626
Fragments arranged by size:
2626 1553 1107 505 499 476 266 182
157 135 70 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3505 5346 5592 669
Size: 988 281 1567 1841 246 3079
Fragments arranged by size:
3079 1841 1567 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8002
HpaI GTT'AAC
Cuts at: 5592 5592
Size: 8002
KasI G'GCGC_C
Cuts at: 3128 3128
Size: 8002
KpnI G_GTAC'C
Cuts at: 5327 5327
Size: 8002
MfeI C'AATT_G
Cuts at: 5601 5601
Size: 8002
MluI A'CGCG_T
Cuts at: 7878 7878
Size: 8002
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2370 3605 3621 4348 4905 7116 7300 1305
Size: 1065 1235 16 727 557 2211 184 2007
Fragments arranged by size:
2211 2007 1235 1065 727 557 184 16
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2876 4208 4398 4793 10
Size: 54 1425 343 1044 1332 190 395 3219
Fragments arranged by size:
3219 1425 1332 1044 395 343 190 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3005 3848 3974 4085 4887 5201 5295
Size: 648 1839 843 126 111 802 314 94
Cuts at: 5295 5960 6319 6478 518
Size: 665 359 159 2042
Fragments arranged by size:
2042 1839 843 802 665 648 359 314
159 126 111 94
MspA1I CmG'CkG
Cuts at: 1606 2550 2607 3077 3104 3196 3295 3580 3702
Size: 944 57 470 27 92 99 285 122
Cuts at: 3702 4018 5050 5418 6095 7243 7488 1606
Size: 316 1032 368 677 1148 245 2120
Fragments arranged by size:
2120 1148 1032 944 677 470 368 316
285 245 122 99 92 57 27
NaeI GCC'GGC
Cuts at: 2429 2555 3769 2429
Size: 126 1214 6662
Fragments arranged by size:
6662 1214 126
NarI GG'CG_CC
Cuts at: 3129 3129
Size: 8002
NcoI C'CATG_G
Cuts at: 513 1065 3729 513
Size: 552 2664 4786
Fragments arranged by size:
4786 2664 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8002
NgoMIV G'CCGG_C
Cuts at: 2427 2553 3767 2427
Size: 126 1214 6662
Fragments arranged by size:
6662 1214 126
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8002
NotI GC'GGCC_GC
Cuts at: 5449 5449
Size: 8002
NspI r_CATG'y
Cuts at: 1132 1229 5360 6905 1132
Size: 97 4131 1545 2229
Fragments arranged by size:
4131 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2699 3773 5249 1348
Size: 1351 1074 1476 4101
Fragments arranged by size:
4101 1476 1351 1074
PciI A'CATG_T
Cuts at: 6901 6901
Size: 8002
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2458 3796 5242 1108
Size: 1350 1338 1446 3868
Fragments arranged by size:
3868 1446 1350 1338
PfoI T'CCnGG_A
Cuts at: 2264 2264
Size: 8002
PmeI GTTT'AAAC
Cuts at: 5306 5306
Size: 8002
PmlI CAC'GTG
Cuts at: 3204 3204
Size: 8002
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6071 6103 7584 7616 6071
Size: 32 1481 32 6457
Fragments arranged by size:
6457 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2566 2667 3739 5066 2566
Size: 101 1072 1327 5502
Fragments arranged by size:
5502 1327 1072 101
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8002
PsiI TTA'TAA
Cuts at: 5572 5572
Size: 8002
PspOMI G'GGCC_C
Cuts at: 2786 2786
Size: 8002
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3509 3541 3509
Size: 32 7970
PstI C_TGCA'G
Cuts at: 830 2232 2562 3585 3717 4087 4107 4593 5354
Size: 1402 330 1023 132 370 20 486 761
Cuts at: 5354 830
Size: 3478
Fragments arranged by size:
3478 1402 1023 761 486 370 330 132
20
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8002
PvuII CAG'CTG
Cuts at: 2550 2607 3077 3104 3196 4018 5050 2550
Size: 57 470 27 92 822 1032 5502
Fragments arranged by size:
5502 1032 822 470 92 57 27
SacI G_AGCT'C
Cuts at: 2598 2682 3609 2598
Size: 84 927 6991
Fragments arranged by size:
6991 927 84
SacII CC_GC'GG
Cuts at: 3703 3703
Size: 8002
SalI G'TCGA_C
Cuts at: 1936 5344 1936
Size: 3408 4594
SanDI GG'GwC_CC
Cuts at: 2667 5066 2667
Size: 2399 5603
SapI GCTCTTCn'nnn_
Cuts at: 3162 3162
Size: 8002
SbfI CC_TGCA'GG
Cuts at: 5354 5354
Size: 8002
ScaI AGT'ACT
Cuts at: 4526 6250 7962 4526
Size: 1724 1712 4566
Fragments arranged by size:
4566 1724 1712
SfcI C'TryA_G
Cuts at: 826 1045 2228 2558 3581 3713 4083 4103 4589
Size: 219 1183 330 1023 132 370 20 486
Cuts at: 4589 5350 6485 7166 7357 826
Size: 761 1135 681 191 1471
Fragments arranged by size:
1471 1183 1135 1023 761 681 486 370
330 219 191 132 20
SfoI GGC'GCC
Cuts at: 3130 3130
Size: 8002
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8002
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8002
SmaI CCC'GGG
Cuts at: 1872 2237 5329 1872
Size: 365 3092 4545
Fragments arranged by size:
4545 3092 365
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2105 6106 7007 7269 820
Size: 197 926 162 4001 901 262 1553
Fragments arranged by size:
4001 1553 926 901 262 197 162
SnaBI TAC'GTA
Cuts at: 493 5793 493
Size: 5300 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8002
SphI G_CATG'C
Cuts at: 1132 5360 1132
Size: 4228 3774
SspI AAT'ATT
Cuts at: 5 52 5926 5
Size: 47 5874 2081
Fragments arranged by size:
5874 2081 47
StuI AGG'CCT
Cuts at: 1517 3655 1517
Size: 2138 5864
StyI C'CwwG_G
Cuts at: 513 1065 3729 4170 5441 513
Size: 552 2664 441 1271 3074
Fragments arranged by size:
3074 2664 1271 552 441
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2712 3553 6029 6046 6199 6384 6829
Size: 1255 1132 841 2476 17 153 185 445
Cuts at: 6829 325
Size: 1498
Fragments arranged by size:
2476 1498 1255 1132 841 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4524 6248 7960
Size: 274 80 33 51 1443 2547 1724 1712
Cuts at: 7960 96
Size: 138
Fragments arranged by size:
2547 1724 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7962 7994 7962
Size: 32 7970
Tth111I GACn'n_nGTC
Cuts at: 1316 2414 2720 3467 1316
Size: 1098 306 747 5851
Fragments arranged by size:
5851 1098 747 306
XbaI T'CTAG_A
Cuts at: 5338 5338
Size: 8002
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2314 3976 4151 2314
Size: 1662 175 6165
Fragments arranged by size:
6165 1662 175
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8002
XmaI C'CCGG_G
Cuts at: 1870 2235 5327 1870
Size: 365 3092 4545
Fragments arranged by size:
4545 3092 365
XmnI GAAnn'nnTTC
Cuts at: 4193 6131 4193
Size: 1938 6064
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2176 5810 276
Size: 53 83 186 936 642 3634 2468
Fragments arranged by size:
3634 2468 936 642 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
DraIII FalI FspAI NruI NsiI PacI PspXI RsrII
SexAI SfiI SrfI SwaI