Restriction map for
pFN21AA1944
(Linear) (Six-base) MAPSORT of: KIAA1944.seq Check: 5922 from: 1 to: 3297
KIAA1944.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1796 1833 3297
Size: 1796 37 1464
Fragments arranged by size:
1796 1464 37
AatII G_ACGT'C
Cuts at: 0 173 3297
Size: 173 3124
AccI GT'mk_AC
Cuts at: 0 413 3297
Size: 413 2884
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 968 3297
Size: 205 763 2329
Fragments arranged by size:
2329 763 205
AhdI GACnn_n'nnGTC
Cuts at: 0 86 3297
Size: 86 3211
AleI CACnn'nnGTG
Cuts at: 0 2080 3297
Size: 2080 1217
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2981 3277 3297
Size: 2981 296 20
Fragments arranged by size:
2981 296 20
AlwNI CAG_nnn'CTG
Cuts at: 0 441 464 1530 1887 1926 2132 3237 3297
Size: 441 23 1066 357 39 206 1105 60
Fragments arranged by size:
1105 1066 441 357 206 60 39 23
ApaI G_GGCC'C
Cuts at: 0 785 3297
Size: 785 2512
ApaLI G'TGCA_C
Cuts at: 0 688 3297
Size: 688 2609
ApoI r'AATT_y
Cuts at: 0 364 939 2248 2386 3136 3297
Size: 364 575 1309 138 750 161
Fragments arranged by size:
1309 750 575 364 161 138
AvaI C'yCGr_G
Cuts at: 0 230 527 2002 3297
Size: 230 297 1475 1295
Fragments arranged by size:
1475 1295 297 230
BamHI G'GATC_C
Cuts at: 0 95 746 3297
Size: 95 651 2551
Fragments arranged by size:
2551 651 95
BanI G'GyrC_C
Cuts at: 0 29 315 1123 1560 2290 2560 3297
Size: 29 286 808 437 730 270 737
Fragments arranged by size:
808 737 730 437 286 270 29
BanII G_rGCy'C
Cuts at: 0 593 677 785 1264 1604 1798 1856 3101
Size: 593 84 108 479 340 194 58 1245
Cuts at: 3101 3297
Size: 196
Fragments arranged by size:
1245 593 479 340 196 194 108 84
58
BbeI G_GCGC'C
Cuts at: 0 1127 3297
Size: 1127 2170
BbsI GAAGACnn'nnnn_
Cuts at: 0 731 1342 1436 2769 2790 3297
Size: 731 611 94 1333 21 507
Fragments arranged by size:
1333 731 611 507 94 21
BbvCI CC'TCA_GC
Cuts at: 0 1754 3010 3297
Size: 1754 1256 287
Fragments arranged by size:
1754 1256 287
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2970 3004 3297
Size: 2970 34 293
Fragments arranged by size:
2970 293 34
BglII A'GATC_T
Cuts at: 0 1250 3297
Size: 1250 2047
BlpI GC'TnA_GC
Cuts at: 0 2044 2737 3297
Size: 2044 693 560
Fragments arranged by size:
2044 693 560
Bme1580I G_kGCm'C
Cuts at: 0 7 320 692 785 1565 1631 2563 3297
Size: 7 313 372 93 780 66 932 734
Fragments arranged by size:
932 780 734 372 313 93 66 7
BmgBI CAC'GTC
Cuts at: 0 909 1229 1434 3297
Size: 909 320 205 1863
Fragments arranged by size:
1863 909 320 205
BmrI ACTGGGnnnn_n'
Cuts at: 0 474 2898 3297
Size: 474 2424 399
Fragments arranged by size:
2424 474 399
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2897 3297
Size: 2897 400
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 607 918 1420 1564 1623 3297
Size: 201 406 311 502 144 59 1674
Fragments arranged by size:
1674 502 406 311 201 144 59
Bpu10I CC'TnA_GC
Cuts at: 0 418 1531 1754 2087 3010 3297
Size: 418 1113 223 333 923 287
Fragments arranged by size:
1113 923 418 333 287 223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 3297
Size: 121 3176
BsaI GGTCTCn'nnnn_
Cuts at: 0 517 1368 1593 3297
Size: 517 851 225 1704
Fragments arranged by size:
1704 851 517 225
BsaAI yAC'GTr
Cuts at: 0 1199 3297
Size: 1199 2098
BsaHI Gr'CG_yC
Cuts at: 0 170 1124 3297
Size: 170 954 2173
Fragments arranged by size:
2173 954 170
BsaWI w'CCGG_w
Cuts at: 0 749 2906 3297
Size: 749 2157 391
Fragments arranged by size:
2157 749 391
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 580 610 2926 2956 3297
Size: 580 30 2316 30 341
Fragments arranged by size:
2316 580 341 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 648 747 1684 1687 1864 1922 2872 2934
Size: 648 99 937 3 177 58 950 62
Cuts at: 2934 2998 3150 3297
Size: 64 152 147
Fragments arranged by size:
950 937 648 177 152 147 99 64
62 58 3
BseYI C'CCAG_C
Cuts at: 0 1791 2132 2644 2664 3297
Size: 1791 341 512 20 633
Fragments arranged by size:
1791 633 512 341 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 1686 2029 3247 3297
Size: 454 1232 343 1218 50
Fragments arranged by size:
1232 1218 454 343 50
BsiEI CG_ry'CG
Cuts at: 0 474 1763 3297
Size: 474 1289 1534
Fragments arranged by size:
1534 1289 474
BsiHKAI G_wGCw'C
Cuts at: 0 593 677 692 1604 3297
Size: 593 84 15 912 1693
Fragments arranged by size:
1693 912 593 84 15
BsmBI CGTCTCn'nnnn_
Cuts at: 0 177 899 1641 3162 3297
Size: 177 722 742 1521 135
Fragments arranged by size:
1521 742 722 177 135
Bsp1286I G_dGCh'C
Cuts at: 0 7 320 593 677 692 785 1264 1565
Size: 7 313 273 84 15 93 479 301
Cuts at: 1565 1604 1631 1798 1856 2563 3101 3297
Size: 39 27 167 58 707 538 196
Fragments arranged by size:
707 538 479 313 301 273 196 167
93 84 58 39 27 15 7
BspEI T'CCGG_A
Cuts at: 0 749 3297
Size: 749 2548
BspHI T'CATG_A
Cuts at: 0 206 2883 3297
Size: 206 2677 414
Fragments arranged by size:
2677 414 206
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 426 1184 1194 1796 1833 3297
Size: 426 758 10 602 37 1464
Fragments arranged by size:
1464 758 602 426 37 10
BsrBI CCG'CTC
Cuts at: 0 1686 3297
Size: 1686 1611
BsrDI GCAATG_nn'
Cuts at: 0 747 2264 2697 3297
Size: 747 1517 433 600
Fragments arranged by size:
1517 747 600 433
BsrFI r'CCGG_y
Cuts at: 0 42 422 548 1762 3297
Size: 42 380 126 1214 1535
Fragments arranged by size:
1535 1214 380 126 42
BstAPI GCAn_nnn'nTGC
Cuts at: 0 315 3297
Size: 315 2982
BstEII G'GTnAC_C
Cuts at: 0 1171 1986 3297
Size: 1171 815 1311
Fragments arranged by size:
1311 1171 815
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1191 2082 2211 3297
Size: 1191 891 129 1086
Fragments arranged by size:
1191 1086 891 129
BstYI r'GATC_y
Cuts at: 0 95 746 1250 1883 1922 3234 3297
Size: 95 651 504 633 39 1312 63
Fragments arranged by size:
1312 651 633 504 95 63 39
Bsu36I CC'TnA_GG
Cuts at: 0 502 3297
Size: 502 2795
BtgI C'CryG_G
Cuts at: 0 617 644 668 1367 1695 1724 3297
Size: 617 27 24 699 328 29 1573
Fragments arranged by size:
1573 699 617 328 29 27 24
BtsI GCAGTG_nn'
Cuts at: 0 2075 2087 2963 3297
Size: 2075 12 876 334
Fragments arranged by size:
2075 876 334 12
ClaI AT'CG_AT
Cuts at: 0 770 1148 3297
Size: 770 378 2149
Fragments arranged by size:
2149 770 378
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 606 641 3297
Size: 606 35 2656
Fragments arranged by size:
2656 606 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 2025 2574 3297
Size: 2025 549 723
Fragments arranged by size:
2025 723 549
EaeI y'GGCC_r
Cuts at: 0 59 578 869 1343 1760 2201 2391 2786
Size: 59 519 291 474 417 441 190 395
Cuts at: 2786 3297
Size: 511
Fragments arranged by size:
519 511 474 441 417 395 291 190
59
EagI C'GGCC_G
Cuts at: 0 1760 3297
Size: 1760 1537
EarI CTCTTCn'nnn_
Cuts at: 0 1157 1661 2341 3297
Size: 1157 504 680 956
Fragments arranged by size:
1157 956 680 504
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1892 3297
Size: 1892 1405
EcoICRI GAG'CTC
Cuts at: 0 591 675 1602 3297
Size: 591 84 927 1695
Fragments arranged by size:
1695 927 591 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 205 607 918 968 1420 1564 1623
Size: 201 4 402 311 50 452 144 59
Cuts at: 1623 3297
Size: 1674
Fragments arranged by size:
1674 452 402 311 201 144 59 50
4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 187 792 2299 2719 3120 3297
Size: 187 605 1507 420 401 177
Fragments arranged by size:
1507 605 420 401 187 177
EcoO109I rG'GnC_Cy
Cuts at: 0 561 662 782 1734 3061 3297
Size: 561 101 120 952 1327 236
Fragments arranged by size:
1327 952 561 236 120 101
EcoRV GAT'ATC
Cuts at: 0 204 3297
Size: 204 3093
FseI GG_CCGG'CC
Cuts at: 0 1766 3297
Size: 1766 1531
HaeII r_GCGC'y
Cuts at: 0 482 1127 3297
Size: 482 645 2170
Fragments arranged by size:
2170 645 482
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 72 104 580 612 1111 1143 1213 1245
Size: 72 32 476 32 499 32 70 32
Cuts at: 1245 1402 1434 1616 1648 1914 1946 3053 3085
Size: 157 32 182 32 266 32 1107 32
Cuts at: 3085 3297
Size: 212
Fragments arranged by size:
1107 499 476 266 212 182 157 72
70 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1500 3297
Size: 1500 1797
KasI G'GCGC_C
Cuts at: 0 1123 3297
Size: 1123 2174
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 365 1600 1616 2343 2900 3297
Size: 365 1235 16 727 557 397
Fragments arranged by size:
1235 727 557 397 365 16
MscI TGG'CCA
Cuts at: 0 871 2203 2393 2788 3297
Size: 871 1332 190 395 509
Fragments arranged by size:
1332 871 509 395 190
MslI CAynn'nnrTG
Cuts at: 0 1000 1843 1969 2080 2882 3196 3290 3297
Size: 1000 843 126 111 802 314 94 7
Fragments arranged by size:
1000 843 802 314 126 111 94 7
MspA1I CmG'CkG
Cuts at: 0 545 602 1072 1099 1191 1290 1575 1697
Size: 545 57 470 27 92 99 285 122
Cuts at: 1697 2013 3045 3297
Size: 316 1032 252
Fragments arranged by size:
1032 545 470 316 285 252 122 99
92 57 27
NaeI GCC'GGC
Cuts at: 0 424 550 1764 3297
Size: 424 126 1214 1533
Fragments arranged by size:
1533 1214 424 126
NarI GG'CG_CC
Cuts at: 0 1124 3297
Size: 1124 2173
NcoI C'CATG_G
Cuts at: 0 1724 3297
Size: 1724 1573
NgoMIV G'CCGG_C
Cuts at: 0 422 548 1762 3297
Size: 422 126 1214 1535
Fragments arranged by size:
1535 1214 422 126
PasI CC'CwG_GG
Cuts at: 0 694 1768 3244 3297
Size: 694 1074 1476 53
Fragments arranged by size:
1476 1074 694 53
PflMI CCAn_nnn'nTGG
Cuts at: 0 453 1791 3237 3297
Size: 453 1338 1446 60
Fragments arranged by size:
1446 1338 453 60
PfoI T'CCnGG_A
Cuts at: 0 259 3297
Size: 259 3038
PmlI CAC'GTG
Cuts at: 0 1199 3297
Size: 1199 2098
PpuMI rG'GwC_Cy
Cuts at: 0 561 662 1734 3061 3297
Size: 561 101 1072 1327 236
Fragments arranged by size:
1327 1072 561 236 101
PspOMI G'GGCC_C
Cuts at: 0 781 3297
Size: 781 2516
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1504 1536 3297
Size: 1504 32 1761
Fragments arranged by size:
1761 1504 32
PstI C_TGCA'G
Cuts at: 0 227 557 1580 1712 2082 2102 2588 3297
Size: 227 330 1023 132 370 20 486 709
Fragments arranged by size:
1023 709 486 370 330 227 132 20
PvuII CAG'CTG
Cuts at: 0 545 602 1072 1099 1191 2013 3045 3297
Size: 545 57 470 27 92 822 1032 252
Fragments arranged by size:
1032 822 545 470 252 92 57 27
SacI G_AGCT'C
Cuts at: 0 593 677 1604 3297
Size: 593 84 927 1693
Fragments arranged by size:
1693 927 593 84
SacII CC_GC'GG
Cuts at: 0 1698 3297
Size: 1698 1599
SanDI GG'GwC_CC
Cuts at: 0 662 3061 3297
Size: 662 2399 236
Fragments arranged by size:
2399 662 236
SapI GCTCTTCn'nnn_
Cuts at: 0 1157 3297
Size: 1157 2140
ScaI AGT'ACT
Cuts at: 0 2521 3297
Size: 2521 776
SfcI C'TryA_G
Cuts at: 0 223 553 1576 1708 2078 2098 2584 3297
Size: 223 330 1023 132 370 20 486 713
Fragments arranged by size:
1023 713 486 370 330 223 132 20
SfoI GGC'GCC
Cuts at: 0 1125 3297
Size: 1125 2172
SmaI CCC'GGG
Cuts at: 0 232 3297
Size: 232 3065
SmlI C'TyrA_G
Cuts at: 0 100 3297
Size: 100 3197
StuI AGG'CCT
Cuts at: 0 1650 3297
Size: 1650 1647
StyI C'CwwG_G
Cuts at: 0 1724 2165 3297
Size: 1724 441 1132
Fragments arranged by size:
1724 1132 441
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 707 1548 3297
Size: 707 841 1749
Fragments arranged by size:
1749 841 707
TatI w'GTAC_w
Cuts at: 0 2519 3297
Size: 2519 778
Tth111I GACn'n_nGTC
Cuts at: 0 409 715 1462 3297
Size: 409 306 747 1835
Fragments arranged by size:
1835 747 409 306
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 309 1971 2146 3297
Size: 309 1662 175 1151
Fragments arranged by size:
1662 1151 309 175
XmaI C'CCGG_G
Cuts at: 0 230 3297
Size: 230 3067
XmnI GAAnn'nnTTC
Cuts at: 0 2188 3297
Size: 2188 1109
ZraI GAC'GTC
Cuts at: 0 171 3297
Size: 171 3126
Enzymes that do cut:
AarI AatII AccI AcuI AhdI AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtsI ClaI CspCI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FseI HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV PasI PflMI
PfoI PmlI PpuMI PspOMI PsrI PstI PvuII SacI
SacII SanDI SapI ScaI SfcI SfoI SmaI SmlI
StuI StyI TaqII TatI Tth111I XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AloI AscI
AseI AsiSI AvrII BaeI BciVI BclI BfrBI BglI
BmtI BsaBI BsiWI BsmI BsrGI BssHII BssSI BstBI
BstZ17I BtgZI DraI DraIII EcoRI FalI FspI FspAI
HindIII HpaI KpnI MfeI MluI NdeI NheI NotI
NruI NsiI NspI PacI PciI PmeI PpiI PshAI
PsiI PspXI PvuI RsrII SalI SbfI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TstI XbaI XhoI