(Circular) (Six-base) MAPSORT of: pFN21AA1779.seq Check: 5170 from: 1 to: 8335 pFN21AA1779 8335 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4396 4396 Size: 8335 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6145 278 Size: 53 83 186 936 4609 2468 Fragments arranged by size: 4609 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5678 1937 Size: 3741 4594 Acc65I G'GTAC_C Cuts at: 5392 5656 5392 Size: 264 8071 AclI AA'CG_TT Cuts at: 1563 6462 6835 1563 Size: 4899 373 3063 Fragments arranged by size: 4899 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2181 2355 2967 3689 4133 4698 4802 6398 Size: 549 174 612 722 444 565 104 1596 Cuts at: 6398 7782 8050 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1596 1384 722 612 565 549 444 268 174 104 AfeI AGC'GCT Cuts at: 4996 5050 4996 Size: 54 8281 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8138 AflIII A'CryG_T Cuts at: 1203 4039 4869 7234 8211 1203 Size: 2836 830 2365 977 1327 Fragments arranged by size: 2836 2365 1327 977 830 AgeI A'CCGG_T Cuts at: 4937 7166 4937 Size: 2229 6106 AhdI GACnn_n'nnGTC Cuts at: 7064 7064 Size: 8335 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8335 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3411 4351 1184 Size: 2227 940 5168 Fragments arranged by size: 5168 2227 940 AlwNI CAG_nnn'CTG Cuts at: 2911 3874 4058 5617 6078 7650 2911 Size: 963 184 1559 461 1572 3596 Fragments arranged by size: 3596 1572 1559 963 461 184 ApaLI G'TGCA_C Cuts at: 2438 4941 6392 7548 2438 Size: 2503 1451 1156 3225 Fragments arranged by size: 3225 2503 1451 1156 ApoI r'AATT_y Cuts at: 1493 3225 4031 4106 5644 5870 6022 8131 1493 Size: 1732 806 75 1538 226 152 2109 1697 Fragments arranged by size: 2109 1732 1697 1538 806 226 152 75 AseI AT'TA_AT Cuts at: 160 1032 6889 160 Size: 872 5857 1606 Fragments arranged by size: 5857 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8335 AvaI C'yCGr_G Cuts at: 1870 1943 2030 2090 4369 5660 1870 Size: 73 87 60 2279 1291 4545 Fragments arranged by size: 4545 2279 1291 87 73 60 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2388 2421 2388 Size: 33 8302 BamHI G'GATC_C Cuts at: 2643 2805 5665 6032 2643 Size: 162 2860 367 4946 Fragments arranged by size: 4946 2860 367 162 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3345 4399 4767 5392 5656 Size: 325 217 627 1558 1054 368 625 264 Cuts at: 5656 7111 618 Size: 1455 1842 Fragments arranged by size: 1842 1558 1455 1054 627 625 368 325 264 217 BanII G_rGCy'C Cuts at: 1391 2090 2563 3146 3591 3865 3990 4050 4376 Size: 699 473 583 445 274 125 60 326 Cuts at: 4376 5340 5654 1391 Size: 964 314 4072 Fragments arranged by size: 4072 964 699 583 473 445 326 314 274 125 60 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2487 2664 3750 5433 5631 928 Size: 971 588 177 1086 1683 198 3632 Fragments arranged by size: 3632 1683 1086 971 588 198 177 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3116 3150 6526 6560 1903 Size: 34 50 34 1095 34 3376 34 3678 Fragments arranged by size: 3678 3376 1095 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4555 6227 7437 4555 Size: 1672 1210 5453 Fragments arranged by size: 5453 1672 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8335 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6946 136 Size: 107 122 71 631 605 5274 1525 Fragments arranged by size: 5274 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8330 8330 Size: 8335 BlpI GC'TnA_GC Cuts at: 2562 3890 5752 2562 Size: 1328 1862 5145 Fragments arranged by size: 5145 1862 1328 Bme1580I G_kGCm'C Cuts at: 1790 2442 3348 4404 4770 4877 4945 6396 7552 Size: 652 906 1056 366 107 68 1451 1156 Cuts at: 7552 1790 Size: 2573 Fragments arranged by size: 2573 1451 1156 1056 906 652 366 107 68 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8335 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2804 5461 6066 Size: 416 53 83 399 24 1387 2657 605 Cuts at: 6066 7024 442 Size: 958 1753 Fragments arranged by size: 2657 1753 1387 958 605 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8335 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4713 4713 Size: 8335 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3115 3313 4833 5365 6995 1239 Size: 1876 198 1520 532 1630 2579 Fragments arranged by size: 2579 1876 1630 1520 532 198 Bpu10I CC'TnA_GC Cuts at: 2046 2930 5367 5499 2046 Size: 884 2437 132 4882 Fragments arranged by size: 4882 2437 884 132 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2369 2508 5520 6460 7325 7623 2369 Size: 139 3012 940 865 298 3081 Fragments arranged by size: 3081 3012 940 865 298 139 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6998 882 Size: 617 5499 2219 Fragments arranged by size: 5499 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3256 4042 4872 6126 493 Size: 711 2052 786 830 1254 2702 Fragments arranged by size: 2702 2052 1254 830 786 711 BsaBI GATnn'nnATC Cuts at: 8329 8329 Size: 8335 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2492 6142 6524 7159 Size: 53 83 186 936 959 3650 382 635 Cuts at: 7159 275 Size: 1451 Fragments arranged by size: 3650 1451 959 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2620 2691 4489 4937 6768 7166 7440 7587 7998 Size: 71 1798 448 1831 398 274 147 411 Cuts at: 7998 2620 Size: 2957 Fragments arranged by size: 2957 1831 1798 448 411 398 274 147 71 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4730 4760 1178 Size: 30 165 30 3327 30 4753 Fragments arranged by size: 4753 3327 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2366 2790 3316 3371 3724 3870 3873 4345 Size: 1180 424 526 55 353 146 3 472 Cuts at: 4345 4751 5504 1186 Size: 406 753 4017 Fragments arranged by size: 4017 1180 753 526 472 424 406 353 146 55 3 BseYI C'CCAG_C Cuts at: 2730 2890 3802 4403 4426 4441 4537 5126 5180 Size: 160 912 601 23 15 96 589 54 Cuts at: 5180 7538 8034 2730 Size: 2358 496 3031 Fragments arranged by size: 3031 2358 912 601 589 496 160 96 54 23 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2586 2924 4470 1163 Size: 573 850 338 1546 5028 Fragments arranged by size: 5028 1546 850 573 338 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4220 4224 5785 6546 7574 1540 Size: 275 186 2219 4 1561 761 1028 2301 Fragments arranged by size: 2301 2219 1561 1028 761 275 186 4 BsiHKAI G_wGCw'C Cuts at: 2320 2442 2563 2620 3618 3865 4945 6396 6481 Size: 122 121 57 998 247 1080 1451 85 Cuts at: 6481 7552 2320 Size: 1071 3103 Fragments arranged by size: 3103 1451 1080 1071 998 247 122 121 85 57 BsmI GAATG_Cn' Cuts at: 5373 5846 5939 5373 Size: 473 93 7769 Fragments arranged by size: 7769 473 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2029 2206 3353 2029 Size: 177 1147 7011 Fragments arranged by size: 7011 1147 177 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2090 2320 2442 2563 2620 3146 3348 Size: 399 300 230 122 121 57 526 202 Cuts at: 3348 3591 3618 3865 3990 4050 4376 4404 4770 Size: 243 27 247 125 60 326 28 366 Cuts at: 4770 4877 4945 5340 5654 6396 6481 7552 1391 Size: 107 68 395 314 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 526 399 395 366 326 314 300 247 243 230 202 125 122 121 107 85 68 60 57 28 27 BspEI T'CCGG_A Cuts at: 2691 7998 2691 Size: 5307 3028 BspHI T'CATG_A Cuts at: 5379 6224 7954 5379 Size: 845 1730 5760 Fragments arranged by size: 5760 1730 845 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2520 2946 3379 4396 5690 844 Size: 1676 426 433 1017 1294 3489 Fragments arranged by size: 3489 1676 1294 1017 433 426 BsrBI CCG'CTC Cuts at: 1394 1823 2080 6222 1394 Size: 429 257 4142 3507 Fragments arranged by size: 4142 3507 429 257 BsrDI GCAATG_nn' Cuts at: 65 1248 4692 6830 7004 65 Size: 1183 3444 2138 174 1396 Fragments arranged by size: 3444 2138 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4223 4292 4937 6979 7166 1700 Size: 110 2413 69 645 2042 187 2869 Fragments arranged by size: 2869 2413 2042 645 187 110 69 BsrGI T'GTAC_A Cuts at: 96 2340 2716 3579 3713 96 Size: 2244 376 863 134 4718 Fragments arranged by size: 4718 2244 863 376 134 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8335 BssSI C'ACGA_G Cuts at: 6395 7407 6395 Size: 1012 7323 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8335 BstBI TT'CG_AA Cuts at: 3136 7139 3136 Size: 4003 4332 BstEII G'GTnAC_C Cuts at: 1184 2894 4956 5172 1184 Size: 1710 2062 216 4347 Fragments arranged by size: 4347 2062 1710 216 BstYI r'GATC_y Cuts at: 1972 2643 2805 3870 4119 5665 6032 6417 6434 Size: 671 162 1065 249 1546 367 385 17 Cuts at: 6434 7886 7972 7984 8330 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1546 1452 1065 671 385 367 346 249 162 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2322 2904 2322 Size: 582 7753 BtgI C'CryG_G Cuts at: 513 1065 2011 2647 3879 4347 4395 4950 5058 Size: 552 946 636 1232 468 48 555 108 Cuts at: 5058 5112 5166 513 Size: 54 54 3682 Fragments arranged by size: 3682 1232 946 636 555 552 468 108 54 54 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3304 3899 487 Size: 683 56 2078 595 4923 Fragments arranged by size: 4923 2078 683 595 56 BtsI GCAGTG_nn' Cuts at: 2550 2886 4921 5851 6645 6665 2550 Size: 336 2035 930 794 20 4220 Fragments arranged by size: 4220 2035 930 794 336 20 ClaI AT'CG_AT Cuts at: 1554 2924 1554 Size: 1370 6965 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8300 DraI TTT'AAA Cuts at: 4036 5639 4036 Size: 1603 6732 DraIII CAC_nnn'GTG Cuts at: 3352 3352 Size: 8335 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4595 7342 809 Size: 507 3279 2747 1802 Fragments arranged by size: 3279 2747 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2358 4221 5595 Size: 54 1425 50 275 18 528 1863 1374 Cuts at: 5595 5782 6671 8300 8 Size: 187 889 1629 43 Fragments arranged by size: 1863 1629 1425 1374 889 528 275 187 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4221 5782 1537 Size: 275 2409 1561 4090 Fragments arranged by size: 4090 2409 1561 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3665 3983 4080 4456 4660 4818 5328 6265 Size: 2312 318 97 376 204 158 510 937 Cuts at: 6265 1353 Size: 3423 Fragments arranged by size: 3423 2312 937 510 376 318 204 158 97 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2132 3383 6922 7296 7442 387 Size: 1064 138 543 1251 3539 374 146 1280 Fragments arranged by size: 3539 1280 1251 1064 543 374 146 138 EcoICRI GAG'CTC Cuts at: 2561 3863 2561 Size: 1302 7033 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2181 2355 2967 3115 3313 3689 4133 Size: 393 549 174 612 148 198 376 444 Cuts at: 4133 4698 4802 4833 5365 6398 6995 7782 8050 Size: 565 104 31 532 1033 597 787 268 Cuts at: 8050 1239 Size: 1524 Fragments arranged by size: 1524 1033 787 612 597 565 549 532 444 393 376 268 198 174 148 104 31 EcoNI CCTnn'n_nnAGG Cuts at: 2903 2903 Size: 8335 EcoO109I rG'GnC_Cy Cuts at: 2056 2908 4411 2056 Size: 852 1503 5980 Fragments arranged by size: 5980 1503 852 EcoRI G'AATT_C Cuts at: 5644 6022 5644 Size: 378 7957 EcoRV GAT'ATC Cuts at: 4665 4665 Size: 8335 FspI TGC'GCA Cuts at: 6841 6841 Size: 8335 HaeII r_GCGC'y Cuts at: 1678 4998 5052 7482 1678 Size: 3320 54 2430 2531 Fragments arranged by size: 3320 2531 2430 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2290 2322 2455 2487 3039 Size: 32 135 32 718 32 133 32 552 Cuts at: 3039 3071 4104 4136 4961 4993 5518 5550 6976 Size: 32 1033 32 825 32 525 32 1426 Cuts at: 6976 7008 7050 7082 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1426 1033 825 718 552 525 135 133 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2509 5679 5925 669 Size: 988 281 571 3170 246 3079 Fragments arranged by size: 3170 3079 988 571 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8335 HpaI GTT'AAC Cuts at: 5925 5925 Size: 8335 KpnI G_GTAC'C Cuts at: 5396 5660 5396 Size: 264 8071 MfeI C'AATT_G Cuts at: 5934 5934 Size: 8335 MluI A'CGCG_T Cuts at: 8211 8211 Size: 8335 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4718 7449 7633 1305 Size: 3413 2731 184 2007 Fragments arranged by size: 3413 2731 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2360 10 Size: 54 1425 343 528 5985 Fragments arranged by size: 5985 1425 528 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3884 5378 6293 6652 6811 518 Size: 648 2718 1494 915 359 159 2042 Fragments arranged by size: 2718 2042 1494 915 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2013 2259 2600 3270 3294 3342 4055 4349 Size: 407 246 341 670 24 48 713 294 Cuts at: 4349 5585 5631 5751 6428 7576 7821 1606 Size: 1236 46 120 677 1148 245 2120 Fragments arranged by size: 2120 1236 1148 713 677 670 407 341 294 246 245 120 48 46 24 NaeI GCC'GGC Cuts at: 4225 4225 Size: 8335 NcoI C'CATG_G Cuts at: 513 1065 4395 5112 513 Size: 552 3330 717 3736 Fragments arranged by size: 3736 3330 717 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8335 NgoMIV G'CCGG_C Cuts at: 4223 4223 Size: 8335 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8335 NotI GC'GGCC_GC Cuts at: 5782 5782 Size: 8335 NspI r_CATG'y Cuts at: 1132 1229 2341 4006 5693 7238 1132 Size: 97 1112 1665 1687 1545 2229 Fragments arranged by size: 2229 1687 1665 1545 1112 97 PasI CC'CwG_GG Cuts at: 1348 2059 2846 4965 1348 Size: 711 787 2119 4718 Fragments arranged by size: 4718 2119 787 711 PciI A'CATG_T Cuts at: 7234 7234 Size: 8335 PflMI CCAn_nnn'nTGG Cuts at: 1108 3833 1108 Size: 2725 5610 PmeI GTTT'AAAC Cuts at: 5639 5639 Size: 8335 PmlI CAC'GTG Cuts at: 3256 4042 4872 3256 Size: 786 830 6719 Fragments arranged by size: 6719 830 786 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4280 4312 6404 6436 7917 7949 4280 Size: 32 2092 32 1481 32 4666 Fragments arranged by size: 4666 2092 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2908 2908 Size: 8335 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8335 PsiI TTA'TAA Cuts at: 5905 5905 Size: 8335 PspXI vC'TCGA_Gb Cuts at: 4369 4369 Size: 8335 PstI C_TGCA'G Cuts at: 830 2106 2642 4232 5687 830 Size: 1276 536 1590 1455 3478 Fragments arranged by size: 3478 1590 1455 1276 536 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8335 PvuII CAG'CTG Cuts at: 2600 5585 5631 2600 Size: 2985 46 5304 Fragments arranged by size: 5304 2985 46 SacI G_AGCT'C Cuts at: 2563 3865 2563 Size: 1302 7033 SacII CC_GC'GG Cuts at: 2014 4350 2014 Size: 2336 5999 SalI G'TCGA_C Cuts at: 1936 5677 1936 Size: 3741 4594 SapI GCTCTTCn'nnn_ Cuts at: 3665 4080 5328 3665 Size: 415 1248 6672 Fragments arranged by size: 6672 1248 415 SbfI CC_TGCA'GG Cuts at: 5687 5687 Size: 8335 ScaI AGT'ACT Cuts at: 6583 8295 6583 Size: 1712 6623 SexAI A'CCwGG_T Cuts at: 5388 5388 Size: 8335 SfcI C'TryA_G Cuts at: 826 1045 2102 2638 4135 4228 5683 6818 7499 Size: 219 1057 536 1497 93 1455 1135 681 Cuts at: 7499 7690 826 Size: 191 1471 Fragments arranged by size: 1497 1471 1455 1135 1057 681 536 219 191 93 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8335 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8335 SmaI CCC'GGG Cuts at: 1872 2032 5662 1872 Size: 160 3630 4545 Fragments arranged by size: 4545 3630 160 SmlI C'TyrA_G Cuts at: 820 1017 1943 2348 2523 4369 5535 6439 7340 Size: 197 926 405 175 1846 1166 904 901 Cuts at: 7340 7602 820 Size: 262 1553 Fragments arranged by size: 1846 1553 1166 926 904 901 405 262 197 175 SnaBI TAC'GTA Cuts at: 493 6126 493 Size: 5633 2702 SpeI A'CTAG_T Cuts at: 152 5246 152 Size: 5094 3241 SphI G_CATG'C Cuts at: 1132 5693 1132 Size: 4561 3774 SspI AAT'ATT Cuts at: 5 52 4178 6259 5 Size: 47 4126 2081 2081 Fragments arranged by size: 4126 2081 2081 47 StuI AGG'CCT Cuts at: 1517 4497 1517 Size: 2980 5355 StyI C'CwwG_G Cuts at: 513 1065 4395 5112 5774 513 Size: 552 3330 717 662 3074 Fragments arranged by size: 3330 3074 717 662 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4091 6362 6379 6532 6717 7162 325 Size: 1255 2511 2271 17 153 185 445 1498 Fragments arranged by size: 2511 2271 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2340 2716 3035 Size: 274 80 33 51 1443 363 376 319 Cuts at: 3035 3579 3713 5416 6581 8293 96 Size: 544 134 1703 1165 1712 138 Fragments arranged by size: 1712 1703 1443 1165 544 376 363 319 274 138 134 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2421 2453 3608 3640 4730 4762 8295 8327 2421 Size: 32 1155 32 1090 32 3533 32 2429 Fragments arranged by size: 3533 2429 1155 1090 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3653 4216 1316 Size: 2337 563 5435 Fragments arranged by size: 5435 2337 563 XbaI T'CTAG_A Cuts at: 2233 5671 2233 Size: 3438 4897 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5474 5474 Size: 8335 XhoI C'TCGA_G Cuts at: 1943 4369 1943 Size: 2426 5909 XmaI C'CCGG_G Cuts at: 1870 2030 5660 1870 Size: 160 3630 4545 Fragments arranged by size: 4545 3630 160 XmnI GAAnn'nnTTC Cuts at: 2778 5203 5316 6464 2778 Size: 2425 113 1148 4649 Fragments arranged by size: 4649 2425 1148 113 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6143 276 Size: 53 83 186 936 4609 2468 Fragments arranged by size: 4609 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI BstXI BstZ17I FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspOMI PsrI RsrII SanDI SfiI SfoI SrfI SwaI