Restriction map for
pFN21AA1761
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# NO CUT ENZYMES ( 99 enzymes) #
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Acc65I AclI AflII AgeI AhdI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BalI
BbeI BbrPI BbvCI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsiEI BsiWI BspDI
BspHI BspLU11I BssHII BssSI Bst1107I
BstBI BstZ17I CelII ClaI DraI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoRV EcoT22I FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI KasI KpnI MamI MfeI
MluI MluNI MscI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI PvuII RcaI SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI XbaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 1639 GACGTC GACGT^C
AccI 1780 GTCTAC GT^[AC][GT]AC
AccIII 314 TCCGGA T^CCGGA
AcyI 1639 GACGTC G[GA]^CG[CT]C
AflIII 1424 ACACGT A^C[GA][CT]GT
Alw44I 885 GTGCAC G^TGCAC
ApaLI 885 GTGCAC G^TGCAC
AspI 2202 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 102 GGCACC G^G[CT][GA]CC
BcgI 238 GCAGAGCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 351 GGATAC GTATCC(6/5)
BsaHI 1639 GACGTC G[GA]^CG[CT]C
BsaI 682 GAGACC GGTCTC(1/5)
BseAI 314 TCCGGA T^CCGGA
BsmBI 1180 GAGACG CGTCTC(1/5)
Bsp1407I 767 TGTACA T^GTACA
BspEI 314 TCCGGA T^CCGGA
BspMI 1960 GCAGGT ACCTGC(4/8)
BsrDI 2261 GCAATG GCAATG(2/0)
BsrGI 767 TGTACA T^GTACA
BstAPI 1923 GCATGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 807 GGTGACC G^GT[ACGT]ACC
BstPI 807 GGTGACC G^GT[ACGT]ACC
CpoI 18 CGGTCCG CG^G[AT]CCG
DrdI 643 GACCTCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 807 GGTGACC G^GT[ACGT]ACC
EcoRI 277 GAATTC G^AATTC
HaeII 2342 GGCGCT [GA]GCGC^[CT]
Hin1I 1639 GACGTC G[GA]^CG[CT]C
MroI 314 TCCGGA T^CCGGA
PflFI 2202 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 650 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1162 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 18 CGGTCCG CG^G[AT]CCG
SacI 241 GAGCTC GAGCT^C
SapI 2497 GAAGAGC GCTCTTC(1/4)
SexAI 1808 ACCTGGT A^CC[AT]GGT
SmaI 97 CCCGGG CCC^GGG
SspBI 767 TGTACA T^GTACA
Tth111I 2202 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 377 CCATGAGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 97 CCCGGG C^CCGGG
XmaI 97 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AlwNI 1010 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
1804 CAGCACCTG
BamHI 317 GGATCC G^GATCC
1110 GGATCC
BsaWI 314 TCCGGA [AT]^CCGG[AT]
365 ACCGGA
BsgI 474 GTGCAG GTGCAG(16/14)
493 GTGCAG
BsmFI 2285 GGGAC GGGAC(10/14)
1510 GTCCC
BsmI 1826 GCATTC GAATGC(1/-1)
2046 GCATTC
BsrBI 1470 CCGCTC CCGCTC(-3/-3)
1105 GAGCGG
BsrFI 6 GCCGGC [GA]^CCGG[CT]
75 GCCGGC
BstXI 620 CCATCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2162 CCAAATACTTGG
Cfr10I 6 GCCGGC [GA]^CCGG[CT]
75 GCCGGC
DraII 650 GGGTCCT [GA]G^G[ACGT]CC[CT]
2099 GGGCCCT
DraIII 1807 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1912 CACTGCGTG
EcoO109I 650 GGGTCCT [GA]G^G[ACGT]CC[CT]
2099 GGGCCCT
KspI 106 CCGCGG CCGC^GG
116 CCGCGG
MseI 1724 TTAA T^TAA
1766 TTAA
NaeI 6 GCCGGC GCC^GGC
75 GCCGGC
NgoMIV 6 GCCGGC G^CCGGC
75 GCCGGC
PaeR7I 434 CTCGAG C^TCGAG
1133 CTCGAG
SacII 106 CCGCGG CCGC^GG
116 CCGCGG
TfiI 288 GAATC G^A[AT]TC
981 GATTC
Tru9I 1724 TTAA T^TAA
1766 TTAA
XhoI 434 CTCGAG C^TCGAG
1133 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AocI ApaI ApoI AspHI
AvaI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EarI Eco81I EcoNI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII