(Circular) (Six-base) MAPSORT of: pFN21AA1761.seq Check: 2672 from: 1 to: 7216 pFN21AA1761 7216 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3956 3956 Size: 7216 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3648 5026 278 Size: 53 83 186 936 2112 1378 2468 Fragments arranged by size: 2468 2112 1378 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3786 4559 1937 Size: 1849 773 4594 Fragments arranged by size: 4594 1849 773 Acc65I G'GTAC_C Cuts at: 4537 4537 Size: 7216 AclI AA'CG_TT Cuts at: 1563 5343 5716 1563 Size: 3780 373 3063 Fragments arranged by size: 3780 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2731 3009 3599 4166 5279 6663 6931 1632 Size: 1099 278 590 567 1113 1384 268 1917 Fragments arranged by size: 1917 1384 1113 1099 590 567 278 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7019 AflIII A'CryG_T Cuts at: 1203 3429 6115 7092 1203 Size: 2226 2686 977 1327 Fragments arranged by size: 2686 2226 1327 977 AgeI A'CCGG_T Cuts at: 6047 6047 Size: 7216 AhdI GACnn_n'nnGTC Cuts at: 5945 5945 Size: 7216 AleI CACnn'nnGTG Cuts at: 1166 3371 3671 1166 Size: 2205 300 4711 Fragments arranged by size: 4711 2205 300 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3534 1184 Size: 2350 4866 AlwNI CAG_nnn'CTG Cuts at: 3020 3814 4959 6531 3020 Size: 794 1145 1572 3705 Fragments arranged by size: 3705 1572 1145 794 ApaI G_GGCC'C Cuts at: 2033 4108 4363 2033 Size: 2075 255 4886 Fragments arranged by size: 4886 2075 255 ApaLI G'TGCA_C Cuts at: 2890 5273 6429 2890 Size: 2383 1156 3677 Fragments arranged by size: 3677 2383 1156 ApoI r'AATT_y Cuts at: 1493 2282 2819 3332 4525 4751 4903 7012 1493 Size: 789 537 513 1193 226 152 2109 1697 Fragments arranged by size: 2109 1697 1193 789 537 513 226 152 AseI AT'TA_AT Cuts at: 160 1032 5770 160 Size: 872 4738 1606 Fragments arranged by size: 4738 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7216 AvaI C'yCGr_G Cuts at: 1870 1943 2102 2439 3138 4541 1870 Size: 73 159 337 699 1403 4545 Fragments arranged by size: 4545 1403 699 337 159 73 BamHI G'GATC_C Cuts at: 2322 3115 4546 4913 2322 Size: 793 1431 367 4625 Fragments arranged by size: 4625 1431 793 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2107 4537 5992 618 Size: 325 217 627 320 2430 1455 1842 Fragments arranged by size: 2430 1842 1455 627 325 320 217 BanII G_rGCy'C Cuts at: 1391 2033 2250 2765 3270 3280 4108 4363 4535 Size: 642 217 515 505 10 828 255 172 Cuts at: 4535 1391 Size: 4072 Fragments arranged by size: 4072 828 642 515 505 255 217 172 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2885 4211 4471 928 Size: 971 986 1326 260 3673 Fragments arranged by size: 3673 1326 986 971 260 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2232 2266 5407 5441 1903 Size: 34 295 34 3141 34 3678 Fragments arranged by size: 3678 3141 295 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2350 5108 6318 2350 Size: 2758 1210 3248 Fragments arranged by size: 3248 2758 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7216 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5827 136 Size: 107 122 71 631 605 4155 1525 Fragments arranged by size: 4155 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7211 7211 Size: 7216 BlpI GC'TnA_GC Cuts at: 4633 4633 Size: 7216 Bme1580I G_kGCm'C Cuts at: 1790 2033 2110 2546 2894 4108 4315 4363 5277 Size: 243 77 436 348 1214 207 48 914 Cuts at: 5277 6433 1790 Size: 1156 2573 Fragments arranged by size: 2573 1214 1156 914 436 348 243 207 77 48 BmgBI CAC'GTC Cuts at: 1318 3432 1318 Size: 2114 5102 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4264 4947 5905 Size: 416 53 83 399 24 2847 683 958 Cuts at: 5905 442 Size: 1753 Fragments arranged by size: 2847 1753 958 683 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7216 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2465 2500 2779 4498 2465 Size: 35 279 1719 5183 Fragments arranged by size: 5183 1719 279 35 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2228 2555 3197 4009 5876 1239 Size: 989 327 642 812 1867 2579 Fragments arranged by size: 2579 1867 989 812 642 327 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2414 2445 3335 3746 4139 5341 6206 6504 2414 Size: 31 890 411 393 1202 865 298 3126 Fragments arranged by size: 3126 1202 890 865 411 393 298 31 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2681 5879 882 Size: 617 1182 3198 2219 Fragments arranged by size: 3198 2219 1182 617 BsaAI yAC'GTr Cuts at: 493 1204 5007 493 Size: 711 3803 2702 Fragments arranged by size: 3803 2702 711 BsaBI GATnn'nnATC Cuts at: 7210 7210 Size: 7216 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3645 5023 5405 6040 Size: 53 83 186 936 2112 1378 382 635 Cuts at: 6040 275 Size: 1451 Fragments arranged by size: 2112 1451 1378 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2319 2370 5649 6047 6321 6468 6879 2319 Size: 51 3279 398 274 147 411 2656 Fragments arranged by size: 3279 2656 411 398 274 147 51 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2201 2231 3461 3491 1178 Size: 30 165 30 798 30 1230 30 4903 Fragments arranged by size: 4903 1230 798 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2324 2519 2924 3167 3476 3671 4319 1186 Size: 1138 195 405 243 309 195 648 4083 Fragments arranged by size: 4083 1138 648 405 309 243 195 195 BseYI C'CCAG_C Cuts at: 2058 2758 3548 3691 3807 6419 6915 2058 Size: 700 790 143 116 2612 496 2359 Fragments arranged by size: 2612 2359 790 700 496 143 116 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2500 2519 1163 Size: 573 764 19 5860 Fragments arranged by size: 5860 764 573 19 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4666 5427 6455 1540 Size: 275 186 2665 761 1028 2301 Fragments arranged by size: 2665 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2250 2894 3069 5277 5362 6433 2250 Size: 644 175 2208 85 1071 3033 Fragments arranged by size: 3033 2208 1071 644 175 85 BsmI GAATG_Cn' Cuts at: 3831 4051 4727 4820 3831 Size: 220 676 93 6227 Fragments arranged by size: 6227 676 220 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3179 3179 Size: 7216 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2110 2250 2546 2765 2894 3069 Size: 399 243 77 140 296 219 129 175 Cuts at: 3069 3270 3280 4108 4315 4363 4535 5277 5362 Size: 201 10 828 207 48 172 742 85 Cuts at: 5362 6433 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 828 742 399 296 243 219 207 201 175 172 140 129 85 77 48 10 BspEI T'CCGG_A Cuts at: 2319 6879 2319 Size: 4560 2656 BspHI T'CATG_A Cuts at: 5105 6835 5105 Size: 1730 5486 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3956 4504 4571 844 Size: 3112 548 67 3489 Fragments arranged by size: 3489 3112 548 67 BsrBI CCG'CTC Cuts at: 1394 1823 3112 3477 5103 1394 Size: 429 1289 365 1626 3507 Fragments arranged by size: 3507 1626 1289 429 365 BsrDI GCAATG_nn' Cuts at: 65 1248 4273 5711 5885 65 Size: 1183 3025 1438 174 1396 Fragments arranged by size: 3025 1438 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2011 2080 5860 6047 1700 Size: 110 201 69 3780 187 2869 Fragments arranged by size: 3780 2869 201 187 110 69 BsrGI T'GTAC_A Cuts at: 96 2772 96 Size: 2676 4540 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7216 BssSI C'ACGA_G Cuts at: 5276 6288 5276 Size: 1012 6204 BstAPI GCAn_nnn'nTGC Cuts at: 794 3934 794 Size: 3140 4076 BstBI TT'CG_AA Cuts at: 6020 6020 Size: 7216 BstEII G'GTnAC_C Cuts at: 1184 2812 1184 Size: 1628 5588 BstXI CCAn_nnnn'nTGG Cuts at: 2632 4174 2632 Size: 1542 5674 BstYI r'GATC_y Cuts at: 1972 2322 3115 3582 4023 4546 4913 5298 5315 Size: 350 793 467 441 523 367 385 17 Cuts at: 5315 6767 6853 6865 7211 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 793 523 467 441 385 367 350 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2651 2967 3654 4434 2651 Size: 316 687 780 5433 Fragments arranged by size: 5433 780 687 316 BtgI C'CryG_G Cuts at: 513 1065 2004 2111 2121 3272 513 Size: 552 939 107 10 1151 4457 Fragments arranged by size: 4457 1151 939 552 107 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6477 Fragments arranged by size: 6477 683 56 BtsI GCAGTG_nn' Cuts at: 2409 2611 2833 3361 3879 3916 4190 4732 5526 Size: 202 222 528 518 37 274 542 794 Cuts at: 5526 5546 2409 Size: 20 4079 Fragments arranged by size: 4079 794 542 528 518 274 222 202 37 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7216 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7181 DraI TTT'AAA Cuts at: 4520 4520 Size: 7216 DraIII CAC_nnn'GTG Cuts at: 3817 3922 3817 Size: 105 7111 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2654 6223 809 Size: 507 1338 3569 1802 Fragments arranged by size: 3569 1802 1338 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4663 5552 7181 Size: 54 1425 50 275 18 2833 889 1629 Cuts at: 7181 8 Size: 43 Fragments arranged by size: 2833 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4663 1537 Size: 275 2851 4090 Fragments arranged by size: 4090 2851 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2417 3138 3144 4497 5146 1353 Size: 1064 721 6 1353 649 3423 Fragments arranged by size: 3423 1353 1064 721 649 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2064 3965 5803 6177 6323 387 Size: 1064 138 475 1901 1838 374 146 1280 Fragments arranged by size: 1901 1838 1280 1064 475 374 146 138 EcoICRI GAG'CTC Cuts at: 2248 2248 Size: 7216 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2228 2555 2731 3009 3197 3599 4009 Size: 393 596 327 176 278 188 402 410 Cuts at: 4009 4166 5279 5876 6663 6931 1239 Size: 157 1113 597 787 268 1524 Fragments arranged by size: 1524 1113 787 597 596 410 402 393 327 278 268 188 176 157 EcoNI CCTnn'n_nnAGG Cuts at: 2534 3486 4433 2534 Size: 952 947 5317 Fragments arranged by size: 5317 952 947 EcoO109I rG'GnC_Cy Cuts at: 2656 4105 2656 Size: 1449 5767 EcoRI G'AATT_C Cuts at: 2282 4525 4903 2282 Size: 2243 378 4595 Fragments arranged by size: 4595 2243 378 FspI TGC'GCA Cuts at: 5722 5722 Size: 7216 HaeII r_GCGC'y Cuts at: 1678 4351 6363 1678 Size: 2673 2012 2531 Fragments arranged by size: 2673 2531 2012 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2486 2518 2680 2712 5857 Size: 32 135 32 914 32 162 32 3145 Cuts at: 5857 5889 5931 5963 1373 Size: 32 42 32 2626 Fragments arranged by size: 3145 2626 914 162 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4560 4806 669 Size: 988 281 2622 246 3079 Fragments arranged by size: 3079 2622 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7216 HpaI GTT'AAC Cuts at: 4806 4806 Size: 7216 KpnI G_GTAC'C Cuts at: 4541 4541 Size: 7216 MfeI C'AATT_G Cuts at: 4815 4815 Size: 7216 MluI A'CGCG_T Cuts at: 7092 7092 Size: 7216 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6330 6514 1305 Size: 5025 184 2007 Fragments arranged by size: 5025 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5394 Fragments arranged by size: 5394 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2981 3305 3371 3671 5174 5533 5692 Size: 648 1815 324 66 300 1503 359 159 Cuts at: 5692 518 Size: 2042 Fragments arranged by size: 2042 1815 1503 648 359 324 300 159 66 MspA1I CmG'CkG Cuts at: 1606 2113 2123 2770 4632 5309 6457 6702 1606 Size: 507 10 647 1862 677 1148 245 2120 Fragments arranged by size: 2120 1862 1148 677 647 507 245 10 NaeI GCC'GGC Cuts at: 2013 2082 2013 Size: 69 7147 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5725 Fragments arranged by size: 5725 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7216 NgoMIV G'CCGG_C Cuts at: 2011 2080 2011 Size: 69 7147 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7216 NotI GC'GGCC_GC Cuts at: 4663 4663 Size: 7216 NspI r_CATG'y Cuts at: 1132 1229 4574 6119 1132 Size: 97 3345 1545 2229 Fragments arranged by size: 3345 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7216 PciI A'CATG_T Cuts at: 6115 6115 Size: 7216 PflMI CCAn_nnn'nTGG Cuts at: 1108 3814 4423 4497 1108 Size: 2706 609 74 3827 Fragments arranged by size: 3827 2706 609 74 PfoI T'CCnGG_A Cuts at: 2391 2504 2532 3903 2391 Size: 113 28 1371 5704 Fragments arranged by size: 5704 1371 113 28 PmeI GTTT'AAAC Cuts at: 4520 4520 Size: 7216 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5285 5317 6798 6830 5285 Size: 32 1481 32 5671 Fragments arranged by size: 5671 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2656 2656 Size: 7216 PshAI GACnn'nnGTC Cuts at: 1613 3171 1613 Size: 1558 5658 PsiI TTA'TAA Cuts at: 4786 4786 Size: 7216 PspOMI G'GGCC_C Cuts at: 2029 4104 4359 2029 Size: 2075 255 4886 Fragments arranged by size: 4886 2075 255 PspXI vC'TCGA_Gb Cuts at: 2439 2439 Size: 7216 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3770 3802 3770 Size: 32 7184 PstI C_TGCA'G Cuts at: 830 2245 2603 2919 3523 3598 4404 4515 4568 Size: 1415 358 316 604 75 806 111 53 Cuts at: 4568 830 Size: 3478 Fragments arranged by size: 3478 1415 806 604 358 316 111 75 53 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7216 RsrII CG'GwC_CG Cuts at: 2024 2024 Size: 7216 SacI G_AGCT'C Cuts at: 2250 2250 Size: 7216 SacII CC_GC'GG Cuts at: 2114 2124 2114 Size: 10 7206 SalI G'TCGA_C Cuts at: 1936 4558 1936 Size: 2622 4594 SapI GCTCTTCn'nnn_ Cuts at: 4497 4497 Size: 7216 SbfI CC_TGCA'GG Cuts at: 4568 4568 Size: 7216 ScaI AGT'ACT Cuts at: 5464 7176 5464 Size: 1712 5504 SexAI A'CCwGG_T Cuts at: 3813 3813 Size: 7216 SfcI C'TryA_G Cuts at: 826 1045 2241 2599 2915 3227 3519 3594 4400 Size: 219 1196 358 316 312 292 75 806 Cuts at: 4400 4511 4564 5699 6380 6571 826 Size: 111 53 1135 681 191 1471 Fragments arranged by size: 1471 1196 1135 806 681 358 316 312 292 219 191 111 75 53 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7216 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7216 SmaI CCC'GGG Cuts at: 1872 2104 4543 1872 Size: 232 2439 4545 Fragments arranged by size: 4545 2439 232 SmlI C'TyrA_G Cuts at: 820 1017 1943 2429 2439 2460 3138 3314 3761 Size: 197 926 486 10 21 678 176 447 Cuts at: 3761 4154 5320 6221 6483 820 Size: 393 1166 901 262 1553 Fragments arranged by size: 1553 1166 926 901 678 486 447 393 262 197 176 21 10 SnaBI TAC'GTA Cuts at: 493 5007 493 Size: 4514 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7216 SphI G_CATG'C Cuts at: 1132 4574 1132 Size: 3442 3774 SspI AAT'ATT Cuts at: 5 52 5140 5 Size: 47 5088 2081 Fragments arranged by size: 5088 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7216 StyI C'CwwG_G Cuts at: 513 1065 2004 3282 3535 4286 4655 513 Size: 552 939 1278 253 751 369 3074 Fragments arranged by size: 3074 1278 939 751 552 369 253 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3249 3616 5243 5260 5413 5598 6043 Size: 1255 1669 367 1627 17 153 185 445 Cuts at: 6043 325 Size: 1498 Fragments arranged by size: 1669 1627 1498 1255 445 367 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2772 3426 5462 Size: 274 80 33 51 1443 795 654 2036 Cuts at: 5462 7174 96 Size: 1712 138 Fragments arranged by size: 2036 1712 1443 795 654 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7176 7208 7176 Size: 32 7184 Tth111I GACn'n_nGTC Cuts at: 1316 4210 1316 Size: 2894 4322 XbaI T'CTAG_A Cuts at: 4552 4552 Size: 7216 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2389 2389 Size: 7216 XhoI C'TCGA_G Cuts at: 1943 2439 3138 1943 Size: 496 699 6021 Fragments arranged by size: 6021 699 496 XmaI C'CCGG_G Cuts at: 1870 2102 4541 1870 Size: 232 2439 4545 Fragments arranged by size: 4545 2439 232 XmnI GAAnn'nnTTC Cuts at: 5345 5345 Size: 7216 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3646 5024 276 Size: 53 83 186 936 2112 1378 2468 Fragments arranged by size: 2468 2112 1378 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PvuII SanDI SfiI SfoI SrfI SwaI