Restriction map for
pFN21AA1761
(Linear) (Six-base) MAPSORT of: KIAA1761.seq Check: 3070 from: 1 to: 2511
KIAA1761.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1951 2511
Size: 1951 560
AatII G_ACGT'C
Cuts at: 0 1643 2511
Size: 1643 868
AccI GT'mk_AC
Cuts at: 0 1781 2511
Size: 1781 730
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 726 1004 1594 2161 2511
Size: 726 278 590 567 350
Fragments arranged by size:
726 590 567 350 278
AflIII A'CryG_T
Cuts at: 0 1424 2511
Size: 1424 1087
AleI CACnn'nnGTG
Cuts at: 0 1366 1666 2511
Size: 1366 300 845
Fragments arranged by size:
1366 845 300
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1529 2511
Size: 1529 982
AlwNI CAG_nnn'CTG
Cuts at: 0 1015 1809 2511
Size: 1015 794 702
Fragments arranged by size:
1015 794 702
ApaI G_GGCC'C
Cuts at: 0 28 2103 2358 2511
Size: 28 2075 255 153
Fragments arranged by size:
2075 255 153 28
ApaLI G'TGCA_C
Cuts at: 0 885 2511
Size: 885 1626
ApoI r'AATT_y
Cuts at: 0 277 814 1327 2511
Size: 277 537 513 1184
Fragments arranged by size:
1184 537 513 277
AvaI C'yCGr_G
Cuts at: 0 97 434 1133 2511
Size: 97 337 699 1378
Fragments arranged by size:
1378 699 337 97
BamHI G'GATC_C
Cuts at: 0 317 1110 2511
Size: 317 793 1401
Fragments arranged by size:
1401 793 317
BanI G'GyrC_C
Cuts at: 0 102 2511
Size: 102 2409
BanII G_rGCy'C
Cuts at: 0 28 245 760 1265 1275 2103 2358 2511
Size: 28 217 515 505 10 828 255 153
Fragments arranged by size:
828 515 505 255 217 153 28 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 880 2206 2466 2511
Size: 880 1326 260 45
Fragments arranged by size:
1326 880 260 45
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 227 261 2511
Size: 227 34 2250
Fragments arranged by size:
2250 227 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 345 2511
Size: 345 2166
Bme1580I G_kGCm'C
Cuts at: 0 28 105 541 889 2103 2310 2358 2511
Size: 28 77 436 348 1214 207 48 153
Fragments arranged by size:
1214 436 348 207 153 77 48 28
BmgBI CAC'GTC
Cuts at: 0 1427 2511
Size: 1427 1084
BmrI ACTGGGnnnn_n'
Cuts at: 0 2259 2511
Size: 2259 252
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 460 495 774 2493 2511
Size: 460 35 279 1719 18
Fragments arranged by size:
1719 460 279 35 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 550 1192 2004 2511
Size: 223 327 642 812 507
Fragments arranged by size:
812 642 507 327 223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 440 1330 1741 2134 2511
Size: 409 31 890 411 393 377
Fragments arranged by size:
890 411 409 393 377 31
BsaI GGTCTCn'nnnn_
Cuts at: 0 676 2511
Size: 676 1835
BsaHI Gr'CG_yC
Cuts at: 0 1640 2511
Size: 1640 871
BsaWI w'CCGG_w
Cuts at: 0 314 365 2511
Size: 314 51 2146
Fragments arranged by size:
2146 314 51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 196 226 1456 1486 2511
Size: 196 30 1230 30 1025
Fragments arranged by size:
1230 1025 196 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 319 514 919 1162 1471 1666 2314 2511
Size: 319 195 405 243 309 195 648 197
Fragments arranged by size:
648 405 319 309 243 197 195 195
BseYI C'CCAG_C
Cuts at: 0 53 753 1543 1686 1802 2511
Size: 53 700 790 143 116 709
Fragments arranged by size:
790 709 700 143 116 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 495 514 2511
Size: 495 19 1997
Fragments arranged by size:
1997 495 19
BsiHKAI G_wGCw'C
Cuts at: 0 245 889 1064 2511
Size: 245 644 175 1447
Fragments arranged by size:
1447 644 245 175
BsmI GAATG_Cn'
Cuts at: 0 1826 2046 2511
Size: 1826 220 465
Fragments arranged by size:
1826 465 220
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1174 2511
Size: 1174 1337
Bsp1286I G_dGCh'C
Cuts at: 0 28 105 245 541 760 889 1064 1265
Size: 28 77 140 296 219 129 175 201
Cuts at: 1265 1275 2103 2310 2358 2511
Size: 10 828 207 48 153
Fragments arranged by size:
828 296 219 207 201 175 153 140
129 77 48 28 10
BspEI T'CCGG_A
Cuts at: 0 314 2511
Size: 314 2197
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1951 2511
Size: 1951 560
BsrBI CCG'CTC
Cuts at: 0 1107 1472 2511
Size: 1107 365 1039
Fragments arranged by size:
1107 1039 365
BsrDI GCAATG_nn'
Cuts at: 0 2268 2511
Size: 2268 243
BsrFI r'CCGG_y
Cuts at: 0 6 75 2511
Size: 6 69 2436
Fragments arranged by size:
2436 69 6
BsrGI T'GTAC_A
Cuts at: 0 767 2511
Size: 767 1744
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1929 2511
Size: 1929 582
BstEII G'GTnAC_C
Cuts at: 0 807 2511
Size: 807 1704
BstXI CCAn_nnnn'nTGG
Cuts at: 0 627 2169 2511
Size: 627 1542 342
Fragments arranged by size:
1542 627 342
BstYI r'GATC_y
Cuts at: 0 317 1110 1577 2018 2511
Size: 317 793 467 441 493
Fragments arranged by size:
793 493 467 441 317
Bsu36I CC'TnA_GG
Cuts at: 0 646 962 1649 2429 2511
Size: 646 316 687 780 82
Fragments arranged by size:
780 687 646 316 82
BtgI C'CryG_G
Cuts at: 0 106 116 1267 2511
Size: 106 10 1151 1244
Fragments arranged by size:
1244 1151 106 10
BtsI GCAGTG_nn'
Cuts at: 0 404 606 828 1356 1874 1911 2185 2511
Size: 404 202 222 528 518 37 274 326
Fragments arranged by size:
528 518 404 326 274 222 202 37
DraIII CAC_nnn'GTG
Cuts at: 0 1812 1917 2511
Size: 1812 105 594
Fragments arranged by size:
1812 594 105
DrdI GACnn_nn'nnGTC
Cuts at: 0 649 2511
Size: 649 1862
EarI CTCTTCn'nnn_
Cuts at: 0 412 1133 1139 2492 2511
Size: 412 721 6 1353 19
Fragments arranged by size:
1353 721 412 19 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 59 1960 2511
Size: 59 1901 551
Fragments arranged by size:
1901 551 59
EcoICRI GAG'CTC
Cuts at: 0 243 2511
Size: 243 2268
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 550 726 1004 1192 1594 2004 2161
Size: 223 327 176 278 188 402 410 157
Cuts at: 2161 2511
Size: 350
Fragments arranged by size:
410 402 350 327 278 223 188 176
157
EcoNI CCTnn'n_nnAGG
Cuts at: 0 529 1481 2428 2511
Size: 529 952 947 83
Fragments arranged by size:
952 947 529 83
EcoO109I rG'GnC_Cy
Cuts at: 0 651 2100 2511
Size: 651 1449 411
Fragments arranged by size:
1449 651 411
EcoRI G'AATT_C
Cuts at: 0 277 2511
Size: 277 2234
HaeII r_GCGC'y
Cuts at: 0 2346 2511
Size: 2346 165
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 481 513 675 707 2511
Size: 481 32 162 32 1804
Fragments arranged by size:
1804 481 162 32 32
MslI CAynn'nnrTG
Cuts at: 0 976 1300 1366 1666 2511
Size: 976 324 66 300 845
Fragments arranged by size:
976 845 324 300 66
MspA1I CmG'CkG
Cuts at: 0 108 118 765 2511
Size: 108 10 647 1746
Fragments arranged by size:
1746 647 108 10
NaeI GCC'GGC
Cuts at: 0 8 77 2511
Size: 8 69 2434
Fragments arranged by size:
2434 69 8
NgoMIV G'CCGG_C
Cuts at: 0 6 75 2511
Size: 6 69 2436
Fragments arranged by size:
2436 69 6
PflMI CCAn_nnn'nTGG
Cuts at: 0 1809 2418 2492 2511
Size: 1809 609 74 19
Fragments arranged by size:
1809 609 74 19
PfoI T'CCnGG_A
Cuts at: 0 386 499 527 1898 2511
Size: 386 113 28 1371 613
Fragments arranged by size:
1371 613 386 113 28
PpuMI rG'GwC_Cy
Cuts at: 0 651 2511
Size: 651 1860
PshAI GACnn'nnGTC
Cuts at: 0 1166 2511
Size: 1166 1345
PspOMI G'GGCC_C
Cuts at: 0 24 2099 2354 2511
Size: 24 2075 255 157
Fragments arranged by size:
2075 255 157 24
PspXI vC'TCGA_Gb
Cuts at: 0 434 2511
Size: 434 2077
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1765 1797 2511
Size: 1765 32 714
Fragments arranged by size:
1765 714 32
PstI C_TGCA'G
Cuts at: 0 240 598 914 1518 1593 2399 2510 2511
Size: 240 358 316 604 75 806 111 1
Fragments arranged by size:
806 604 358 316 240 111 75 1
RsrII CG'GwC_CG
Cuts at: 0 19 2511
Size: 19 2492
SacI G_AGCT'C
Cuts at: 0 245 2511
Size: 245 2266
SacII CC_GC'GG
Cuts at: 0 109 119 2511
Size: 109 10 2392
Fragments arranged by size:
2392 109 10
SapI GCTCTTCn'nnn_
Cuts at: 0 2492 2511
Size: 2492 19
SexAI A'CCwGG_T
Cuts at: 0 1808 2511
Size: 1808 703
SfcI C'TryA_G
Cuts at: 0 236 594 910 1222 1514 1589 2395 2506
Size: 236 358 316 312 292 75 806 111
Cuts at: 2506 2511
Size: 5
Fragments arranged by size:
806 358 316 312 292 236 111 75
5
SmaI CCC'GGG
Cuts at: 0 99 2511
Size: 99 2412
SmlI C'TyrA_G
Cuts at: 0 424 434 455 1133 1309 1756 2149 2511
Size: 424 10 21 678 176 447 393 362
Fragments arranged by size:
678 447 424 393 362 176 21 10
StyI C'CwwG_G
Cuts at: 0 1277 1530 2281 2511
Size: 1277 253 751 230
Fragments arranged by size:
1277 751 253 230
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1244 1611 2511
Size: 1244 367 900
Fragments arranged by size:
1244 900 367
TatI w'GTAC_w
Cuts at: 0 767 1421 2511
Size: 767 654 1090
Fragments arranged by size:
1090 767 654
Tth111I GACn'n_nGTC
Cuts at: 0 2205 2511
Size: 2205 306
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 384 2511
Size: 384 2127
XhoI C'TCGA_G
Cuts at: 0 434 1133 2511
Size: 434 699 1378
Fragments arranged by size:
1378 699 434
XmaI C'CCGG_G
Cuts at: 0 97 2511
Size: 97 2414
ZraI GAC'GTC
Cuts at: 0 1641 2511
Size: 1641 870
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI
BcgI BciVI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI
DraIII DrdI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI HaeII Hin4I MslI MspA1I NaeI NgoMIV PflMI
PfoI PpuMI PshAI PspOMI PspXI PsrI PstI RsrII
SacI SacII SapI SexAI SfcI SmaI SmlI StyI
TaqII TatI Tth111I XcmI XhoI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AloI AscI
AseI AsiSI AvrII BaeI BbeI BbvCI BclI BfrBI
BglI BglII BlpI BmtI Bpu10I BsaAI BsaBI BsiEI
BsiWI BspHI BssHII BssSI BstBI BstZ17I BtgZI ClaI
CspCI DraI EaeI EagI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NarI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PsiI PvuI PvuII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI XbaI XmnI