(Circular) (Six-base) MAPSORT of: pFN21AA1760.seq Check: 7608 from: 1 to: 11335 pFN21AA1760 11335 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4245 5816 6937 8281 4245 Size: 1571 1121 1344 7299 Fragments arranged by size: 7299 1571 1344 1121 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3099 3537 4734 5067 Size: 53 83 186 936 1563 438 1197 333 Cuts at: 5067 6260 7281 9145 278 Size: 1193 1021 1864 2468 Fragments arranged by size: 2468 1864 1563 1197 1193 1021 936 438 333 186 83 53 AccI GT'mk_AC Cuts at: 1937 2589 7281 8678 1937 Size: 652 4692 1397 4594 Fragments arranged by size: 4692 4594 1397 652 Acc65I G'GTAC_C Cuts at: 8318 8656 8318 Size: 338 10997 AclI AA'CG_TT Cuts at: 1563 5696 9462 9835 1563 Size: 4133 3766 373 3063 Fragments arranged by size: 4133 3766 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2831 2843 2900 3440 4307 7697 7844 8207 Size: 1199 12 57 540 867 3390 147 363 Cuts at: 8207 8408 8429 9398 10782 11050 1632 Size: 201 21 969 1384 268 1917 Fragments arranged by size: 3390 1917 1384 1199 969 867 540 363 268 201 147 57 21 12 AfeI AGC'GCT Cuts at: 2081 2262 2081 Size: 181 11154 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 11138 AflIII A'CryG_T Cuts at: 1203 5486 5541 7015 10234 11211 1203 Size: 4283 55 1474 3219 977 1327 Fragments arranged by size: 4283 3219 1474 1327 977 55 AgeI A'CCGG_T Cuts at: 10166 10166 Size: 11335 AhdI GACnn_n'nnGTC Cuts at: 6019 10064 6019 Size: 4045 7290 AleI CACnn'nnGTG Cuts at: 1166 3335 3528 1166 Size: 2169 193 8973 Fragments arranged by size: 8973 2169 193 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4127 4341 7766 1184 Size: 2943 214 3425 4753 Fragments arranged by size: 4753 3425 2943 214 AlwNI CAG_nnn'CTG Cuts at: 2173 2357 2878 3958 4192 5032 5808 6064 6200 Size: 184 521 1080 234 840 776 256 136 Cuts at: 6200 6238 6347 7660 8252 8425 9078 10650 2173 Size: 38 109 1313 592 173 653 1572 2858 Fragments arranged by size: 2858 1572 1313 1080 840 776 653 592 521 256 234 184 173 136 109 38 ApaI G_GGCC'C Cuts at: 2031 2076 2156 3942 4124 4276 5268 6080 6525 Size: 45 80 1786 182 152 992 812 445 Cuts at: 6525 7403 7526 2031 Size: 878 123 5840 Fragments arranged by size: 5840 1786 992 878 812 445 182 152 123 80 45 ApaLI G'TGCA_C Cuts at: 3224 9392 10548 3224 Size: 6168 1156 4011 Fragments arranged by size: 6168 4011 1156 ApoI r'AATT_y Cuts at: 1493 8644 8870 9022 11131 1493 Size: 7151 226 152 2109 1697 Fragments arranged by size: 7151 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 9889 160 Size: 872 8857 1606 Fragments arranged by size: 8857 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11335 AvaI C'yCGr_G Cuts at: 1870 1943 2012 2049 2309 2456 3044 3059 3713 Size: 73 69 37 260 147 588 15 654 Cuts at: 3713 3728 5267 5933 6251 6855 7131 7404 7506 Size: 15 1539 666 318 604 276 273 102 Cuts at: 7506 7526 7625 8490 8660 1870 Size: 20 99 865 170 4545 Fragments arranged by size: 4545 1539 865 666 654 604 588 318 276 273 260 170 147 102 99 73 69 37 20 15 15 AvrII C'CTAG_G Cuts at: 8200 8200 Size: 11335 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4679 4712 4679 Size: 33 11302 BamHI G'GATC_C Cuts at: 4063 8665 9032 4063 Size: 4602 367 6366 Fragments arranged by size: 6366 4602 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2146 2865 3055 3667 4309 Size: 325 217 627 359 719 190 612 642 Cuts at: 4309 4561 4720 5426 5819 6097 7780 8318 8656 Size: 252 159 706 393 278 1683 538 338 Cuts at: 8656 10111 618 Size: 1455 1842 Fragments arranged by size: 1842 1683 1455 719 706 642 627 612 538 393 359 338 325 278 252 217 190 159 BanII G_rGCy'C Cuts at: 1391 2013 2031 2076 2156 2284 2293 2316 2334 Size: 622 18 45 80 128 9 23 18 Cuts at: 2334 2457 2481 3351 3729 3827 3942 4016 4124 Size: 123 24 870 378 98 115 74 108 Cuts at: 4124 4276 4974 5157 5268 6080 6252 6384 6408 Size: 152 698 183 111 812 172 132 24 Cuts at: 6408 6525 6723 7026 7071 7374 7403 7526 7626 Size: 117 198 303 45 303 29 123 100 Cuts at: 7626 7950 8654 1391 Size: 324 704 4072 Fragments arranged by size: 4072 870 812 704 698 622 378 324 303 303 198 183 172 152 132 128 123 123 117 115 111 108 100 98 80 74 45 45 29 24 24 23 18 18 9 BbeI G_GCGC'C Cuts at: 2150 3059 4313 4565 4724 5430 2150 Size: 909 1254 252 159 706 8055 Fragments arranged by size: 8055 1254 909 706 252 159 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2819 3411 8016 928 Size: 971 920 592 4605 4247 Fragments arranged by size: 4605 4247 971 920 592 BbvCI CC'TCA_GC Cuts at: 5801 6667 6673 5801 Size: 866 6 10463 Fragments arranged by size: 10463 866 6 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4162 4196 5627 5661 9526 9560 1903 Size: 34 2225 34 1431 34 3865 34 3678 Fragments arranged by size: 3865 3678 2225 1431 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 9227 10437 9227 Size: 1210 10125 BclI T'GATC_A Cuts at: 1548 1548 Size: 11335 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5263 5936 6075 Size: 107 122 71 631 605 3591 673 139 Cuts at: 6075 6113 6209 7740 9946 136 Size: 38 96 1531 2206 1525 Fragments arranged by size: 3591 2206 1531 1525 673 631 605 139 122 107 96 71 38 BglII A'GATC_T Cuts at: 3174 7683 11330 3174 Size: 4509 3647 3179 Fragments arranged by size: 4509 3647 3179 BlpI GC'TnA_GC Cuts at: 3305 4281 5744 5919 8044 8752 3305 Size: 976 1463 175 2125 708 5888 Fragments arranged by size: 5888 2125 1463 976 708 175 Bme1580I G_kGCm'C Cuts at: 1790 2031 2076 2156 2324 3228 3672 3942 4124 Size: 241 45 80 168 904 444 270 182 Cuts at: 4124 4276 4368 5214 5268 5822 6080 6100 6373 Size: 152 92 846 54 554 258 20 273 Cuts at: 6373 6525 6660 6881 7091 7403 7526 8583 9396 Size: 152 135 221 210 312 123 1057 813 Cuts at: 9396 10552 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1057 904 846 813 554 444 312 273 270 258 241 221 210 182 168 152 152 135 123 92 80 54 45 20 BmgBI CAC'GTC Cuts at: 1318 6882 7312 8281 8513 1318 Size: 5564 430 969 232 4140 Fragments arranged by size: 5564 4140 969 430 232 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2976 3901 5562 Size: 416 53 83 399 24 1559 925 1661 Cuts at: 5562 6773 7582 9066 10024 442 Size: 1211 809 1484 958 1753 Fragments arranged by size: 1753 1661 1559 1484 1211 958 925 809 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 7110 1056 Size: 6054 5281 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5347 6367 6814 7315 7521 5347 Size: 1020 447 501 206 9161 Fragments arranged by size: 9161 1020 501 447 206 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2684 3143 3488 4031 4202 4239 4280 4452 Size: 1445 459 345 543 171 37 41 172 Cuts at: 4452 4961 4989 5468 5600 6147 6318 6324 6575 Size: 509 28 479 132 547 171 6 251 Cuts at: 6575 6737 7071 7285 7861 7885 9995 1239 Size: 162 334 214 576 24 2110 2579 Fragments arranged by size: 2579 2110 1445 576 547 543 509 479 459 345 334 251 214 172 171 171 162 132 41 37 28 24 6 Bpu10I CC'TnA_GC Cuts at: 2451 4968 5073 5151 5801 6065 6402 6667 6673 Size: 2517 105 78 650 264 337 265 6 Cuts at: 6673 7920 7970 8253 2451 Size: 1247 50 283 5533 Fragments arranged by size: 5533 2517 1247 650 337 283 265 264 105 78 50 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2495 2531 4314 7847 9460 10325 10623 2495 Size: 36 1783 3533 1613 865 298 3207 Fragments arranged by size: 3533 3207 1783 1613 865 298 36 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2603 2922 6802 7000 7405 9998 882 Size: 617 1104 319 3880 198 405 2593 2219 Fragments arranged by size: 3880 2593 2219 1104 617 405 319 198 BsaAI yAC'GTr Cuts at: 493 1204 5652 6655 7016 9126 493 Size: 711 4448 1003 361 2110 2702 Fragments arranged by size: 4448 2702 2110 1003 711 361 BsaBI GATnn'nnATC Cuts at: 11329 11329 Size: 11335 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2147 2236 2301 2909 Size: 53 83 186 936 614 89 65 608 Cuts at: 2909 3056 3096 3479 3534 4310 4562 4721 4731 Size: 147 40 383 55 776 252 159 10 Cuts at: 4731 5064 5427 5760 6257 6363 7278 7433 9142 Size: 333 363 333 497 106 915 155 1709 Cuts at: 9142 9524 10159 275 Size: 382 635 1451 Fragments arranged by size: 1709 1451 936 915 776 635 614 608 497 383 382 363 333 333 252 186 159 155 147 106 89 83 65 55 53 40 10 BsaWI w'CCGG_w Cuts at: 2646 2957 5911 9768 10166 10440 10587 10998 2646 Size: 311 2954 3857 398 274 147 411 2983 Fragments arranged by size: 3857 2983 2954 411 398 311 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3269 3299 4423 4453 5756 Size: 30 165 30 1866 30 1124 30 1303 Cuts at: 5756 5786 6322 6352 8021 8051 1178 Size: 30 536 30 1669 30 4462 Fragments arranged by size: 4462 1866 1669 1303 1124 536 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2132 2411 2501 2504 3086 3371 3746 4146 Size: 946 279 90 3 582 285 375 400 Cuts at: 4146 5336 5843 6112 6655 7336 1186 Size: 1190 507 269 543 681 5185 Fragments arranged by size: 5185 1190 946 681 582 543 507 400 375 285 279 269 90 3 BseYI C'CCAG_C Cuts at: 2076 2563 3704 3967 4100 4167 4516 4674 4699 Size: 487 1141 263 133 67 349 158 25 Cuts at: 4699 5044 5171 6038 6080 6103 6407 6498 6677 Size: 345 127 867 42 23 304 91 179 Cuts at: 6677 6819 6974 7519 7535 7992 8265 8451 8521 Size: 142 155 545 16 457 273 186 70 Cuts at: 8521 10538 11034 2076 Size: 2017 496 2377 Fragments arranged by size: 2377 2017 1141 867 545 496 487 457 349 345 304 273 263 186 179 158 155 142 133 127 91 70 67 42 25 23 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2882 3937 3978 4778 5222 5864 6320 Size: 573 1146 1055 41 800 444 642 456 Cuts at: 6320 6381 6996 7442 8006 8261 8321 1163 Size: 61 615 446 564 255 60 4177 Fragments arranged by size: 4177 1146 1055 800 642 615 573 564 456 446 444 255 61 60 41 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2069 2412 2439 2539 4428 5767 Size: 275 186 68 343 27 100 1889 1339 Cuts at: 5767 8785 9546 10574 1540 Size: 3018 761 1028 2301 Fragments arranged by size: 3018 2301 1889 1339 1028 761 343 275 186 100 68 27 BsiHKAI G_wGCw'C Cuts at: 3078 3228 3351 5157 5925 6252 6654 7626 8143 Size: 150 123 1806 768 327 402 972 517 Cuts at: 8143 9396 9481 10552 3078 Size: 1253 85 1071 3861 Fragments arranged by size: 3861 1806 1253 1071 972 768 517 402 327 150 123 85 BsmI GAATG_Cn' Cuts at: 3169 8846 8939 3169 Size: 5677 93 5565 Fragments arranged by size: 5677 5565 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3470 6248 6264 6912 8198 8617 3470 Size: 2778 16 648 1286 419 6188 Fragments arranged by size: 6188 2778 1286 648 419 16 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2031 2076 2156 2284 2293 2316 Size: 399 223 18 45 80 128 9 23 Cuts at: 2316 2324 2334 2457 2481 3078 3228 3351 3672 Size: 8 10 123 24 597 150 123 321 Cuts at: 3672 3729 3827 3942 4016 4124 4276 4368 4974 Size: 57 98 115 74 108 152 92 606 Cuts at: 4974 5157 5214 5268 5822 5925 6080 6100 6252 Size: 183 57 54 554 103 155 20 152 Cuts at: 6252 6373 6384 6408 6525 6654 6660 6723 6881 Size: 121 11 24 117 129 6 63 158 Cuts at: 6881 7026 7071 7091 7374 7403 7526 7626 7950 Size: 145 45 20 283 29 123 100 324 Cuts at: 7950 8143 8583 8654 9396 9481 10552 1391 Size: 193 440 71 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 606 597 554 440 399 324 321 283 223 193 183 158 155 152 152 150 145 129 128 123 123 123 121 117 115 108 103 100 98 92 85 80 74 71 63 57 57 54 45 45 29 24 24 23 20 20 18 11 10 9 8 6 BspEI T'CCGG_A Cuts at: 10998 10998 Size: 11335 BspHI T'CATG_A Cuts at: 9224 10954 9224 Size: 1730 9605 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3309 3667 4177 4245 4428 5469 5816 6937 Size: 2465 358 510 68 183 1041 347 1121 Cuts at: 6937 7003 7018 8281 8343 8690 844 Size: 66 15 1263 62 347 3489 Fragments arranged by size: 3489 2465 1263 1121 1041 510 358 347 347 183 68 66 62 15 BsrBI CCG'CTC Cuts at: 1394 1823 2668 4280 5072 5689 6744 7974 9222 Size: 429 845 1612 792 617 1055 1230 1248 Cuts at: 9222 1394 Size: 3507 Fragments arranged by size: 3507 1612 1248 1230 1055 845 792 617 429 BsrDI GCAATG_nn' Cuts at: 65 1248 9830 10004 65 Size: 1183 8582 174 1396 Fragments arranged by size: 8582 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2303 2868 3205 3664 4006 4222 4603 Size: 110 493 565 337 459 342 216 381 Cuts at: 4603 4786 4948 5098 5517 7511 9979 10166 1700 Size: 183 162 150 419 1994 2468 187 2869 Fragments arranged by size: 2869 2468 1994 565 493 459 419 381 342 337 216 187 183 162 150 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 11335 BssHII G'CGCG_C Cuts at: 1925 2224 5492 1925 Size: 299 3268 7768 Fragments arranged by size: 7768 3268 299 BssSI C'ACGA_G Cuts at: 3524 6281 6308 8136 9395 10407 3524 Size: 2757 27 1828 1259 1012 4452 Fragments arranged by size: 4452 2757 1828 1259 1012 27 BstAPI GCAn_nnn'nTGC Cuts at: 794 2173 2769 2865 3757 3958 4915 5209 7660 Size: 1379 596 96 892 201 957 294 2451 Cuts at: 7660 794 Size: 4469 Fragments arranged by size: 4469 2451 1379 957 892 596 294 201 96 BstBI TT'CG_AA Cuts at: 10139 10139 Size: 11335 BstEII G'GTnAC_C Cuts at: 1184 3187 3685 5602 1184 Size: 2003 498 1917 6917 Fragments arranged by size: 6917 2003 1917 498 BstXI CCAn_nnnn'nTGG Cuts at: 2530 3790 4556 5179 5656 7195 7543 8239 2530 Size: 1260 766 623 477 1539 348 696 5626 Fragments arranged by size: 5626 1539 1260 766 696 623 477 348 BstYI r'GATC_y Cuts at: 1972 2874 3174 3597 4063 7683 8665 9032 9417 Size: 902 300 423 466 3620 982 367 385 Cuts at: 9417 9434 10886 10972 10984 11330 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 3620 1977 1452 982 902 466 423 385 367 346 300 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2800 3389 6231 6786 2800 Size: 589 2842 555 7349 Fragments arranged by size: 7349 2842 589 555 BtgI C'CryG_G Cuts at: 513 1065 2004 2031 2295 2316 2517 2569 3090 Size: 552 939 27 264 21 201 52 521 Cuts at: 3090 3364 3987 4116 4206 4668 5184 5767 8544 Size: 274 623 129 90 462 516 583 2777 Cuts at: 8544 513 Size: 3304 Fragments arranged by size: 3304 2777 939 623 583 552 521 516 462 274 264 201 129 90 52 27 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2200 2332 3367 487 Size: 683 56 974 132 1035 8455 Fragments arranged by size: 8455 1035 974 683 132 56 BtsI GCAGTG_nn' Cuts at: 4790 4808 4952 6477 7329 7748 7950 7989 8851 Size: 18 144 1525 852 419 202 39 862 Cuts at: 8851 9645 9665 4790 Size: 794 20 6460 Fragments arranged by size: 6460 1525 862 852 794 419 202 144 39 20 18 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 11335 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3750 3785 529 Size: 35 3186 35 8079 Fragments arranged by size: 8079 3186 35 35 DraI TTT'AAA Cuts at: 8639 8639 Size: 11335 DraIII CAC_nnn'GTG Cuts at: 4756 4756 Size: 11335 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6901 8119 10342 809 Size: 507 5585 1218 2223 1802 Fragments arranged by size: 5585 2223 1802 1218 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2066 2436 2520 Size: 54 1425 50 275 18 236 370 84 Cuts at: 2520 2536 2997 3129 3207 3546 3817 4068 4144 Size: 16 461 132 78 339 271 251 76 Cuts at: 4144 4569 4605 4623 5038 5181 6489 7792 8071 Size: 425 36 18 415 143 1308 1303 279 Cuts at: 8071 8782 9671 11300 8 Size: 711 889 1629 43 Fragments arranged by size: 1629 1425 1308 1303 889 711 461 425 415 370 339 279 275 271 251 236 143 132 84 78 76 54 50 43 36 18 18 16 EagI C'GGCC_G Cuts at: 1537 1812 2066 2436 2536 8782 1537 Size: 275 254 370 100 6246 4090 Fragments arranged by size: 6246 4090 370 275 254 100 EarI CTCTTCn'nnn_ Cuts at: 1353 2478 2679 5885 9265 1353 Size: 1125 201 3206 3380 3423 Fragments arranged by size: 3423 3380 3206 1125 201 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2057 3651 8076 9922 10296 10442 Size: 1064 138 468 1594 4425 1846 374 146 Cuts at: 10442 387 Size: 1280 Fragments arranged by size: 4425 1846 1594 1280 1064 468 374 146 138 EcoICRI GAG'CTC Cuts at: 3349 5155 6250 7624 3349 Size: 1806 1095 1374 7060 Fragments arranged by size: 7060 1806 1374 1095 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2684 2831 2843 2900 3143 3440 3488 Size: 393 1052 147 12 57 243 297 48 Cuts at: 3488 4031 4202 4239 4280 4307 4452 4961 4989 Size: 543 171 37 41 27 145 509 28 Cuts at: 4989 5468 5600 6147 6318 6324 6575 6737 7071 Size: 479 132 547 171 6 251 162 334 Cuts at: 7071 7285 7697 7844 7861 7885 8207 8408 8429 Size: 214 412 147 17 24 322 201 21 Cuts at: 8429 9398 9995 10782 11050 1239 Size: 969 597 787 268 1524 Fragments arranged by size: 1524 1052 969 787 597 547 543 509 479 412 393 334 322 297 268 251 243 214 201 171 171 162 147 147 145 132 57 48 41 37 28 27 24 21 17 12 6 EcoNI CCTnn'n_nnAGG Cuts at: 3470 4184 6068 6355 7289 8256 8493 3470 Size: 714 1884 287 934 967 237 6312 Fragments arranged by size: 6312 1884 967 934 714 287 237 EcoO109I rG'GnC_Cy Cuts at: 2027 2072 2073 2884 3938 3972 4189 4272 4273 Size: 45 1 811 1054 34 217 83 1 Cuts at: 4273 5077 5938 6077 6286 6423 6521 6681 6763 Size: 804 861 139 209 137 98 160 82 Cuts at: 6763 7143 7294 7399 7400 7523 7555 7910 7924 Size: 380 151 105 1 123 32 355 14 Cuts at: 7924 8223 8249 8261 2027 Size: 299 26 12 5101 Fragments arranged by size: 5101 1054 861 811 804 380 355 299 217 209 160 151 139 137 123 105 98 83 82 45 34 32 26 14 12 1 1 1 EcoRI G'AATT_C Cuts at: 8644 9022 8644 Size: 378 10957 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5635 8338 5635 Size: 2703 8632 FspI TGC'GCA Cuts at: 9841 9841 Size: 11335 HaeII r_GCGC'y Cuts at: 1678 2083 2150 2182 2264 2757 3059 3783 3896 Size: 405 67 32 82 493 302 724 113 Cuts at: 3896 4313 4565 4724 5430 6005 7131 8528 10482 Size: 417 252 159 706 575 1126 1397 1954 Cuts at: 10482 1678 Size: 2531 Fragments arranged by size: 2531 1954 1397 1126 724 706 575 493 417 405 302 252 159 113 82 67 32 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3025 3057 4423 4455 5756 Size: 32 135 32 1453 32 1366 32 1301 Cuts at: 5756 5788 6005 6037 6176 6208 7163 7195 7361 Size: 32 217 32 139 32 955 32 166 Cuts at: 7361 7393 7708 7740 8021 8053 8473 8497 8505 Size: 32 315 32 281 32 420 24 8 Cuts at: 8505 8529 9976 10008 10050 10082 1373 Size: 24 1447 32 42 32 2626 Fragments arranged by size: 2626 1453 1447 1366 1301 955 420 315 281 217 166 139 135 42 32 32 32 32 32 32 32 32 32 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 8679 8925 669 Size: 988 281 6741 246 3079 Fragments arranged by size: 6741 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 11335 HpaI GTT'AAC Cuts at: 8925 8925 Size: 11335 KasI G'GCGC_C Cuts at: 2146 3055 4309 4561 4720 5426 2146 Size: 909 1254 252 159 706 8055 Fragments arranged by size: 8055 1254 909 706 252 159 KpnI G_GTAC'C Cuts at: 8322 8660 8322 Size: 338 10997 MfeI C'AATT_G Cuts at: 8934 8934 Size: 11335 MluI A'CGCG_T Cuts at: 5486 11211 5486 Size: 5725 5610 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2088 5799 6540 10449 10633 1305 Size: 783 3711 741 3909 184 2007 Fragments arranged by size: 3909 3711 2007 783 741 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2522 2999 3131 3548 4571 Size: 54 1425 343 690 477 132 417 1023 Cuts at: 4571 5040 5183 6491 8073 10 Size: 469 143 1308 1582 3272 Fragments arranged by size: 3272 1582 1425 1308 1023 690 477 469 417 343 143 132 54 MslI CAynn'nnrTG Cuts at: 518 1166 3081 3335 3528 4109 5654 9293 9652 Size: 648 1915 254 193 581 1545 3639 359 Cuts at: 9652 9811 518 Size: 159 2042 Fragments arranged by size: 3639 2042 1915 1545 648 581 359 254 193 159 MspA1I CmG'CkG Cuts at: 1606 2033 2297 2357 2571 2674 2862 3816 4162 Size: 427 264 60 214 103 188 954 346 Cuts at: 4162 4285 4703 5009 5254 5299 5418 5578 6019 Size: 123 418 306 245 45 119 160 441 Cuts at: 6019 6420 6677 6814 8386 8407 8751 9428 10576 Size: 401 257 137 1572 21 344 677 1148 Cuts at: 10576 10821 1606 Size: 245 2120 Fragments arranged by size: 2120 1572 1148 954 677 441 427 418 401 346 344 306 264 257 245 245 214 188 160 137 123 119 103 60 45 21 NaeI GCC'GGC Cuts at: 2305 2870 3207 4788 4950 5519 7513 2305 Size: 565 337 1581 162 569 1994 6127 Fragments arranged by size: 6127 1994 1581 569 565 337 162 NarI GG'CG_CC Cuts at: 2147 3056 4310 4562 4721 5427 2147 Size: 909 1254 252 159 706 8055 Fragments arranged by size: 8055 1254 909 706 252 159 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 9844 Fragments arranged by size: 9844 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 11335 NgoMIV G'CCGG_C Cuts at: 2303 2868 3205 4786 4948 5517 7511 2303 Size: 565 337 1581 162 569 1994 6127 Fragments arranged by size: 6127 1994 1581 569 565 337 162 NheI G'CTAG_C Cuts at: 1052 7106 1052 Size: 6054 5281 NotI GC'GGCC_GC Cuts at: 8782 8782 Size: 11335 NspI r_CATG'y Cuts at: 1132 1229 3121 5743 8693 10238 1132 Size: 97 1892 2622 2950 1545 2229 Fragments arranged by size: 2950 2622 2229 1892 1545 97 PasI CC'CwG_GG Cuts at: 1348 2391 4556 5780 6517 6582 1348 Size: 1043 2165 1224 737 65 6101 Fragments arranged by size: 6101 2165 1224 1043 737 65 PciI A'CATG_T Cuts at: 10234 10234 Size: 11335 PflMI CCAn_nnn'nTGG Cuts at: 1108 5599 6826 1108 Size: 4491 1227 5617 Fragments arranged by size: 5617 4491 1227 PfoI T'CCnGG_A Cuts at: 4059 8554 4059 Size: 4495 6840 PmeI GTTT'AAAC Cuts at: 8639 8639 Size: 11335 PmlI CAC'GTG Cuts at: 6655 7016 6655 Size: 361 10974 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 9404 9436 10917 10949 9404 Size: 32 1481 32 9790 Fragments arranged by size: 9790 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4189 5077 6423 6681 6763 7143 7555 7924 8223 Size: 888 1346 258 82 380 412 369 299 Cuts at: 8223 4189 Size: 7301 Fragments arranged by size: 7301 1346 888 412 380 369 299 258 82 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 11335 PsiI TTA'TAA Cuts at: 8905 8905 Size: 11335 PspOMI G'GGCC_C Cuts at: 2027 2072 2152 3938 4120 4272 5264 6076 6521 Size: 45 80 1786 182 152 992 812 445 Cuts at: 6521 7399 7522 2027 Size: 878 123 5840 Fragments arranged by size: 5840 1786 992 878 812 445 182 152 123 80 45 PspXI vC'TCGA_Gb Cuts at: 6855 7131 7625 8490 6855 Size: 276 494 865 9700 Fragments arranged by size: 9700 865 494 276 PstI C_TGCA'G Cuts at: 830 2178 2643 2712 3615 3678 4320 4533 5682 Size: 1348 465 69 903 63 642 213 1149 Cuts at: 5682 7497 7656 7698 7957 8687 830 Size: 1815 159 42 259 730 3478 Fragments arranged by size: 3478 1815 1348 1149 903 730 642 465 259 213 159 69 63 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11335 PvuII CAG'CTG Cuts at: 2862 4285 5009 5299 5418 5578 6019 6677 6814 Size: 1423 724 290 119 160 441 658 137 Cuts at: 6814 8386 8407 2862 Size: 1572 21 5790 Fragments arranged by size: 5790 1572 1423 724 658 441 290 160 137 119 21 SacI G_AGCT'C Cuts at: 3351 5157 6252 7626 3351 Size: 1806 1095 1374 7060 Fragments arranged by size: 7060 1806 1374 1095 SacII CC_GC'GG Cuts at: 2034 2298 2572 2034 Size: 264 274 10797 Fragments arranged by size: 10797 274 264 SalI G'TCGA_C Cuts at: 1936 8677 1936 Size: 6741 4594 SanDI GG'GwC_CC Cuts at: 6423 6681 7143 6423 Size: 258 462 10615 Fragments arranged by size: 10615 462 258 SbfI CC_TGCA'GG Cuts at: 3678 5682 8687 3678 Size: 2004 3005 6326 Fragments arranged by size: 6326 3005 2004 ScaI AGT'ACT Cuts at: 9583 11295 9583 Size: 1712 9623 SexAI A'CCwGG_T Cuts at: 8587 8587 Size: 11335 SfcI C'TryA_G Cuts at: 826 1045 2174 2639 2708 3611 3674 4316 4529 Size: 219 1129 465 69 903 63 642 213 Cuts at: 4529 5678 7493 7652 7694 7953 8683 9818 10499 Size: 1149 1815 159 42 259 730 1135 681 Cuts at: 10499 10690 826 Size: 191 1471 Fragments arranged by size: 1815 1471 1149 1135 1129 903 730 681 642 465 259 219 213 191 159 69 63 42 SfoI GGC'GCC Cuts at: 2148 3057 4311 4563 4722 5428 2148 Size: 909 1254 252 159 706 8055 Fragments arranged by size: 8055 1254 909 706 252 159 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11335 SgrAI Cr'CCGG_yG Cuts at: 1700 3664 1700 Size: 1964 9371 SmaI CCC'GGG Cuts at: 1872 2014 2458 3715 5269 8662 1872 Size: 142 444 1257 1554 3393 4545 Fragments arranged by size: 4545 3393 1554 1257 444 142 SmlI C'TyrA_G Cuts at: 820 1017 1943 2510 2546 4329 6251 6855 7131 Size: 197 926 567 36 1783 1922 604 276 Cuts at: 7131 7625 7862 8490 9439 10340 10602 820 Size: 494 237 628 949 901 262 1553 Fragments arranged by size: 1922 1783 1553 949 926 901 628 604 567 494 276 262 237 197 36 SnaBI TAC'GTA Cuts at: 493 9126 493 Size: 8633 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 11335 SphI G_CATG'C Cuts at: 1132 3121 8693 1132 Size: 1989 5572 3774 Fragments arranged by size: 5572 3774 1989 SrfI GCCC'GGGC Cuts at: 2014 2458 3715 2014 Size: 444 1257 9634 Fragments arranged by size: 9634 1257 444 SspI AAT'ATT Cuts at: 5 52 2947 9259 5 Size: 47 2895 6312 2081 Fragments arranged by size: 6312 2895 2081 47 StuI AGG'CCT Cuts at: 1517 2706 5330 7501 1517 Size: 1189 2624 2171 5351 Fragments arranged by size: 5351 2624 2171 1189 StyI C'CwwG_G Cuts at: 513 1065 2004 2757 2847 3432 3618 6374 6438 Size: 552 939 753 90 585 186 2756 64 Cuts at: 6438 6825 8074 8200 8774 513 Size: 387 1249 126 574 3074 Fragments arranged by size: 3074 2756 1249 939 753 585 574 552 387 186 126 90 64 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2605 2929 3388 5058 5231 7549 8109 Size: 1255 1025 324 459 1670 173 2318 560 Cuts at: 8109 8635 9362 9379 9532 9717 10162 325 Size: 526 727 17 153 185 445 1498 Fragments arranged by size: 2318 1670 1498 1255 1025 727 560 526 459 445 324 185 173 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3834 9581 11293 Size: 274 80 33 51 1443 1857 5747 1712 Cuts at: 11293 96 Size: 138 Fragments arranged by size: 5747 1857 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2904 2936 5465 5497 6322 6354 6933 6965 8536 Size: 32 2529 32 825 32 579 32 1571 Cuts at: 8536 8568 11295 11327 2904 Size: 32 2727 32 2912 Fragments arranged by size: 2912 2727 2529 1571 825 579 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 8572 1316 Size: 7256 4079 XbaI T'CTAG_A Cuts at: 8671 8671 Size: 11335 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3701 4378 5033 5656 6613 8236 3701 Size: 677 655 623 957 1623 6800 Fragments arranged by size: 6800 1623 957 677 655 623 XhoI C'TCGA_G Cuts at: 1943 6251 6855 7131 7625 8490 1943 Size: 4308 604 276 494 865 4788 Fragments arranged by size: 4788 4308 865 604 494 276 XmaI C'CCGG_G Cuts at: 1870 2012 2456 3713 5267 8660 1870 Size: 142 444 1257 1554 3393 4545 Fragments arranged by size: 4545 3393 1554 1257 444 142 XmnI GAAnn'nnTTC Cuts at: 2828 9464 2828 Size: 6636 4699 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3097 3535 4732 5065 Size: 53 83 186 936 1563 438 1197 333 Cuts at: 5065 6258 7279 9143 276 Size: 1193 1021 1864 2468 Fragments arranged by size: 2468 1864 1563 1197 1193 1021 936 438 333 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BfrBI BsiWI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PsrI RsrII SapI SfiI SwaI