(Circular) (Six-base) MAPSORT of: pFN21AA1700.seq Check: 6549 from: 1 to: 6700 pFN21AA1700 6700 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2360 2370 2360 Size: 10 6690 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4510 278 Size: 53 83 186 936 2974 2468 Fragments arranged by size: 2974 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4043 1937 Size: 2106 4594 Acc65I G'GTAC_C Cuts at: 4021 4021 Size: 6700 AclI AA'CG_TT Cuts at: 1563 4827 5200 1563 Size: 3264 373 3063 Fragments arranged by size: 3264 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2286 3417 4763 6147 6415 1632 Size: 654 1131 1346 1384 268 1917 Fragments arranged by size: 1917 1384 1346 1131 654 268 AflII C'TTAA_G Cuts at: 820 1017 3611 820 Size: 197 2594 3909 Fragments arranged by size: 3909 2594 197 AflIII A'CryG_T Cuts at: 1203 2796 5599 6576 1203 Size: 1593 2803 977 1327 Fragments arranged by size: 2803 1593 1327 977 AgeI A'CCGG_T Cuts at: 5531 5531 Size: 6700 AhdI GACnn_n'nnGTC Cuts at: 5429 5429 Size: 6700 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6700 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3470 3779 1184 Size: 2286 309 4105 Fragments arranged by size: 4105 2286 309 AlwNI CAG_nnn'CTG Cuts at: 3672 3885 4443 6015 3672 Size: 213 558 1572 4357 Fragments arranged by size: 4357 1572 558 213 ApaLI G'TGCA_C Cuts at: 2729 4757 5913 2729 Size: 2028 1156 3516 Fragments arranged by size: 3516 2028 1156 ApoI r'AATT_y Cuts at: 1493 2487 2658 3896 4009 4235 4387 6496 1493 Size: 994 171 1238 113 226 152 2109 1697 Fragments arranged by size: 2109 1697 1238 994 226 171 152 113 AseI AT'TA_AT Cuts at: 160 1032 2142 5254 160 Size: 872 1110 3112 1606 Fragments arranged by size: 3112 1606 1110 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6700 AvaI C'yCGr_G Cuts at: 1870 1943 2609 4025 1870 Size: 73 666 1416 4545 Fragments arranged by size: 4545 1416 666 73 BamHI G'GATC_C Cuts at: 4030 4397 4030 Size: 367 6333 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3482 4021 5476 618 Size: 325 217 627 1695 539 1455 1842 Fragments arranged by size: 1842 1695 1455 627 539 325 217 BanII G_rGCy'C Cuts at: 1391 2208 3857 4019 1391 Size: 817 1649 162 4072 Fragments arranged by size: 4072 1649 817 162 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3183 928 Size: 971 1284 4445 Fragments arranged by size: 4445 1284 971 BbvCI CC'TCA_GC Cuts at: 3712 3712 Size: 6700 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4891 4925 1903 Size: 34 2954 34 3678 Fragments arranged by size: 3678 2954 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4592 5802 4592 Size: 1210 5490 BclI T'GATC_A Cuts at: 1548 1548 Size: 6700 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3242 3615 5311 Size: 107 122 71 631 605 1570 373 1696 Cuts at: 5311 136 Size: 1525 Fragments arranged by size: 1696 1570 1525 631 605 373 122 107 71 BglII A'GATC_T Cuts at: 6695 6695 Size: 6700 BlpI GC'TnA_GC Cuts at: 4117 4117 Size: 6700 Bme1580I G_kGCm'C Cuts at: 1790 2733 3087 3096 4761 5917 1790 Size: 943 354 9 1665 1156 2573 Fragments arranged by size: 2573 1665 1156 943 354 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6700 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4431 5389 442 Size: 416 53 83 399 24 3014 958 1753 Fragments arranged by size: 3014 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6700 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2543 2543 Size: 6700 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2357 2931 2972 5360 1239 Size: 1118 574 41 2388 2579 Fragments arranged by size: 2579 2388 1118 574 41 Bpu10I CC'TnA_GC Cuts at: 3712 3712 Size: 6700 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2921 3176 4825 5690 5988 2921 Size: 255 1649 865 298 3633 Fragments arranged by size: 3633 1649 865 298 255 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3771 4000 5363 882 Size: 617 2272 229 1363 2219 Fragments arranged by size: 2272 2219 1363 617 229 BsaAI yAC'GTr Cuts at: 493 1204 4491 493 Size: 711 3287 2702 Fragments arranged by size: 3287 2702 711 BsaBI GATnn'nnATC Cuts at: 6694 6694 Size: 6700 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3008 4507 4889 5524 Size: 53 83 186 936 1475 1499 382 635 Cuts at: 5524 275 Size: 1451 Fragments arranged by size: 1499 1475 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5133 5531 5805 5952 6363 5133 Size: 398 274 147 411 5470 Fragments arranged by size: 5470 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2833 2863 3768 3798 1178 Size: 30 165 30 1430 30 905 30 4080 Fragments arranged by size: 4080 1430 905 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3256 1186 Size: 2070 4630 BseYI C'CCAG_C Cuts at: 2741 3086 3095 3940 5903 6399 2741 Size: 345 9 845 1963 496 3042 Fragments arranged by size: 3042 1963 845 496 345 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2930 3122 3137 1163 Size: 573 1194 192 15 4726 Fragments arranged by size: 4726 1194 573 192 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4150 4911 5939 1540 Size: 275 186 2149 761 1028 2301 Fragments arranged by size: 2301 2149 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2208 2733 4761 4846 5917 2208 Size: 525 2028 85 1071 2991 Fragments arranged by size: 2991 2028 1071 525 85 BsmI GAATG_Cn' Cuts at: 2215 3457 4211 4304 2215 Size: 1242 754 93 4611 Fragments arranged by size: 4611 1242 754 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2999 2999 Size: 6700 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2208 2733 3087 3096 3857 4019 4761 Size: 399 418 525 354 9 761 162 742 Cuts at: 4761 4846 5917 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 761 742 525 418 399 354 162 85 9 BspEI T'CCGG_A Cuts at: 6363 6363 Size: 6700 BspHI T'CATG_A Cuts at: 2781 4589 6319 2781 Size: 1808 1730 3162 Fragments arranged by size: 3162 1808 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2360 2370 2447 4055 844 Size: 1516 10 77 1608 3489 Fragments arranged by size: 3489 1608 1516 77 10 BsrBI CCG'CTC Cuts at: 1394 1823 2754 4587 1394 Size: 429 931 1833 3507 Fragments arranged by size: 3507 1833 931 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5195 5369 65 Size: 1183 3947 174 1396 Fragments arranged by size: 3947 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2097 5344 5531 1700 Size: 110 287 3247 187 2869 Fragments arranged by size: 3247 2869 287 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6700 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6700 BssSI C'ACGA_G Cuts at: 4760 5772 4760 Size: 1012 5688 BstAPI GCAn_nnn'nTGC Cuts at: 794 3885 794 Size: 3091 3609 BstBI TT'CG_AA Cuts at: 5504 5504 Size: 6700 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6700 BstXI CCAn_nnnn'nTGG Cuts at: 3310 3310 Size: 6700 BstYI r'GATC_y Cuts at: 1972 2745 4030 4397 4782 4799 6251 6337 6349 Size: 773 1285 367 385 17 1452 86 12 Cuts at: 6349 6695 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1285 773 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2666 513 Size: 552 939 662 4547 Fragments arranged by size: 4547 939 662 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2749 487 Size: 683 56 1523 4438 Fragments arranged by size: 4438 1523 683 56 BtsI GCAGTG_nn' Cuts at: 3484 3509 3736 4216 5010 5030 3484 Size: 25 227 480 794 20 5154 Fragments arranged by size: 5154 794 480 227 25 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6700 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6665 DraI TTT'AAA Cuts at: 4004 4004 Size: 6700 DraIII CAC_nnn'GTG Cuts at: 3026 3026 Size: 6700 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5707 809 Size: 507 4391 1802 Fragments arranged by size: 4391 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2099 4147 5036 Size: 54 1425 50 275 18 269 2048 889 Cuts at: 5036 6665 8 Size: 1629 43 Fragments arranged by size: 2048 1629 1425 889 275 269 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4147 1537 Size: 275 2335 4090 Fragments arranged by size: 4090 2335 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2305 2336 2780 3843 3930 4630 1353 Size: 952 31 444 1063 87 700 3423 Fragments arranged by size: 3423 1063 952 700 444 87 31 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2746 3850 5287 5661 5807 387 Size: 1064 138 1157 1104 1437 374 146 1280 Fragments arranged by size: 1437 1280 1157 1104 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2206 2206 Size: 6700 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2286 2357 2931 2972 3417 4763 5360 Size: 393 654 71 574 41 445 1346 597 Cuts at: 5360 6147 6415 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1346 787 654 597 574 445 393 268 71 41 EcoNI CCTnn'n_nnAGG Cuts at: 2531 2531 Size: 6700 EcoO109I rG'GnC_Cy Cuts at: 3495 3495 Size: 6700 EcoRI G'AATT_C Cuts at: 2487 4009 4387 2487 Size: 1522 378 4800 Fragments arranged by size: 4800 1522 378 EcoRV GAT'ATC Cuts at: 3214 3631 3214 Size: 417 6283 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2617 3217 2617 Size: 600 6100 FspI TGC'GCA Cuts at: 3797 5206 3797 Size: 1409 5291 HaeII r_GCGC'y Cuts at: 1678 3143 5847 1678 Size: 1465 2704 2531 Fragments arranged by size: 2704 2531 1465 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2011 2043 2261 2276 2293 Size: 32 135 32 439 32 218 15 17 Cuts at: 2293 2308 3203 3235 3768 3800 3894 3911 3926 Size: 15 895 32 533 32 94 17 15 Cuts at: 3926 3943 5341 5373 5415 5447 1373 Size: 17 1398 32 42 32 2626 Fragments arranged by size: 2626 1398 895 533 439 218 135 94 42 32 32 32 32 32 32 32 17 17 17 15 15 15 HincII GTy'rAC Cuts at: 669 1657 1938 2236 4044 4290 669 Size: 988 281 298 1808 246 3079 Fragments arranged by size: 3079 1808 988 298 281 246 HindIII A'AGCT_T Cuts at: 748 2159 2811 748 Size: 1411 652 4637 Fragments arranged by size: 4637 1411 652 HpaI GTT'AAC Cuts at: 4290 4290 Size: 6700 KpnI G_GTAC'C Cuts at: 4025 4025 Size: 6700 MfeI C'AATT_G Cuts at: 4299 4299 Size: 6700 MluI A'CGCG_T Cuts at: 6576 6576 Size: 6700 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2652 5814 5998 1305 Size: 1347 3162 184 2007 Fragments arranged by size: 3162 2007 1347 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4878 Fragments arranged by size: 4878 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3519 4658 5017 5176 518 Size: 648 2353 1139 359 159 2042 Fragments arranged by size: 2353 2042 1139 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3130 3672 3753 3763 4116 4793 5941 6186 Size: 1524 542 81 10 353 677 1148 245 Cuts at: 6186 1606 Size: 2120 Fragments arranged by size: 2120 1524 1148 677 542 353 245 81 10 NaeI GCC'GGC Cuts at: 2099 2099 Size: 6700 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5209 Fragments arranged by size: 5209 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6700 NgoMIV G'CCGG_C Cuts at: 2097 2097 Size: 6700 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6700 NotI GC'GGCC_GC Cuts at: 4147 4147 Size: 6700 NspI r_CATG'y Cuts at: 1132 1229 2800 4058 5603 1132 Size: 97 1571 1258 1545 2229 Fragments arranged by size: 2229 1571 1545 1258 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6700 PciI A'CATG_T Cuts at: 2796 5599 2796 Size: 2803 3897 PflMI CCAn_nnn'nTGG Cuts at: 1108 2131 2878 3672 1108 Size: 1023 747 794 4136 Fragments arranged by size: 4136 1023 794 747 PfoI T'CCnGG_A Cuts at: 2877 2877 Size: 6700 PmeI GTTT'AAAC Cuts at: 4004 4004 Size: 6700 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4769 4801 6282 6314 4769 Size: 32 1481 32 5155 Fragments arranged by size: 5155 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3495 3495 Size: 6700 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6700 PsiI TTA'TAA Cuts at: 4270 4270 Size: 6700 PstI C_TGCA'G Cuts at: 830 3420 3758 4052 830 Size: 2590 338 294 3478 Fragments arranged by size: 3478 2590 338 294 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6700 PvuII CAG'CTG Cuts at: 3672 3753 3763 3672 Size: 81 10 6609 Fragments arranged by size: 6609 81 10 SacI G_AGCT'C Cuts at: 2208 2208 Size: 6700 SalI G'TCGA_C Cuts at: 1936 4042 1936 Size: 2106 4594 SapI GCTCTTCn'nnn_ Cuts at: 2336 3930 2336 Size: 1594 5106 SbfI CC_TGCA'GG Cuts at: 4052 4052 Size: 6700 ScaI AGT'ACT Cuts at: 4948 6660 4948 Size: 1712 4988 SfcI C'TryA_G Cuts at: 826 1045 3416 3748 3754 4048 5183 5864 6055 Size: 219 2371 332 6 294 1135 681 191 Cuts at: 6055 826 Size: 1471 Fragments arranged by size: 2371 1471 1135 681 332 294 219 191 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6700 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6700 SmaI CCC'GGG Cuts at: 1872 4027 1872 Size: 2155 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2936 3191 3611 4804 5705 5967 Size: 197 926 993 255 420 1193 901 262 Cuts at: 5967 820 Size: 1553 Fragments arranged by size: 1553 1193 993 926 901 420 262 255 197 SnaBI TAC'GTA Cuts at: 493 4491 493 Size: 3998 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6700 SphI G_CATG'C Cuts at: 1132 4058 1132 Size: 2926 3774 SspI AAT'ATT Cuts at: 5 52 4624 5 Size: 47 4572 2081 Fragments arranged by size: 4572 2081 47 StuI AGG'CCT Cuts at: 1517 3430 1517 Size: 1913 4787 StyI C'CwwG_G Cuts at: 513 1065 2004 4139 513 Size: 552 939 2135 3074 Fragments arranged by size: 3074 2135 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3451 4727 4744 4897 5082 5527 325 Size: 1255 1871 1276 17 153 185 445 1498 Fragments arranged by size: 1871 1498 1276 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2320 4946 6658 Size: 274 80 33 51 1443 343 2626 1712 Cuts at: 6658 96 Size: 138 Fragments arranged by size: 2626 1712 1443 343 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6660 6692 6660 Size: 32 6668 Tth111I GACn'n_nGTC Cuts at: 1316 3782 1316 Size: 2466 4234 XbaI T'CTAG_A Cuts at: 4036 4036 Size: 6700 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2018 3896 2018 Size: 1878 4822 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6700 XmaI C'CCGG_G Cuts at: 1870 4025 1870 Size: 2155 4545 XmnI GAAnn'nnTTC Cuts at: 2345 4829 2345 Size: 2484 4216 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4508 276 Size: 53 83 186 936 2974 2468 Fragments arranged by size: 2974 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI