Restriction map for
pFN21AA1700
(Linear) (Six-base) MAPSORT of: KIAA1700.seq Check: 2137 from: 1 to: 1995
KIAA1700.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 355 365 1995
Size: 355 10 1630
Fragments arranged by size:
1630 355 10
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 1412 1995
Size: 281 1131 583
Fragments arranged by size:
1131 583 281
AflII C'TTAA_G
Cuts at: 0 1606 1995
Size: 1606 389
AflIII A'CryG_T
Cuts at: 0 791 1995
Size: 791 1204
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1465 1774 1995
Size: 1465 309 221
Fragments arranged by size:
1465 309 221
AlwNI CAG_nnn'CTG
Cuts at: 0 1667 1880 1995
Size: 1667 213 115
Fragments arranged by size:
1667 213 115
ApaLI G'TGCA_C
Cuts at: 0 724 1995
Size: 724 1271
ApoI r'AATT_y
Cuts at: 0 482 653 1891 1995
Size: 482 171 1238 104
Fragments arranged by size:
1238 482 171 104
AseI AT'TA_AT
Cuts at: 0 137 1995
Size: 137 1858
AvaI C'yCGr_G
Cuts at: 0 604 1995
Size: 604 1391
BanI G'GyrC_C
Cuts at: 0 1477 1995
Size: 1477 518
BanII G_rGCy'C
Cuts at: 0 203 1852 1995
Size: 203 1649 143
Fragments arranged by size:
1649 203 143
BbsI GAAGACnn'nnnn_
Cuts at: 0 1178 1995
Size: 1178 817
BbvCI CC'TCA_GC
Cuts at: 0 1707 1995
Size: 1707 288
BglI GCCn_nnn'nGGC
Cuts at: 0 1237 1610 1995
Size: 1237 373 385
Fragments arranged by size:
1237 385 373
Bme1580I G_kGCm'C
Cuts at: 0 728 1082 1091 1995
Size: 728 354 9 904
Fragments arranged by size:
904 728 354 9
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 538 1995
Size: 538 1457
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 926 967 1995
Size: 352 574 41 1028
Fragments arranged by size:
1028 574 352 41
Bpu10I CC'TnA_GC
Cuts at: 0 1707 1995
Size: 1707 288
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 916 1171 1995
Size: 916 255 824
Fragments arranged by size:
916 824 255
BsaI GGTCTCn'nnnn_
Cuts at: 0 1766 1995
Size: 1766 229
BsaHI Gr'CG_yC
Cuts at: 0 1003 1995
Size: 1003 992
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 828 858 1763 1793 1995
Size: 828 30 905 30 202
Fragments arranged by size:
905 828 202 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1251 1995
Size: 1251 744
BseYI C'CCAG_C
Cuts at: 0 736 1081 1090 1935 1995
Size: 736 345 9 845 60
Fragments arranged by size:
845 736 345 60 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 925 1117 1132 1995
Size: 925 192 15 863
Fragments arranged by size:
925 863 192 15
BsiHKAI G_wGCw'C
Cuts at: 0 203 728 1995
Size: 203 525 1267
Fragments arranged by size:
1267 525 203
BsmI GAATG_Cn'
Cuts at: 0 210 1452 1995
Size: 210 1242 543
Fragments arranged by size:
1242 543 210
BsmBI CGTCTCn'nnnn_
Cuts at: 0 994 1995
Size: 994 1001
Bsp1286I G_dGCh'C
Cuts at: 0 203 728 1082 1091 1852 1995
Size: 203 525 354 9 761 143
Fragments arranged by size:
761 525 354 203 143 9
BspHI T'CATG_A
Cuts at: 0 776 1995
Size: 776 1219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 355 365 442 1995
Size: 355 10 77 1553
Fragments arranged by size:
1553 355 77 10
BsrBI CCG'CTC
Cuts at: 0 749 1995
Size: 749 1246
BsrFI r'CCGG_y
Cuts at: 0 92 1995
Size: 92 1903
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1880 1995
Size: 1880 115
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1305 1995
Size: 1305 690
BstYI r'GATC_y
Cuts at: 0 740 1995
Size: 740 1255
BtgI C'CryG_G
Cuts at: 0 661 1995
Size: 661 1334
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 744 1995
Size: 744 1251
BtsI GCAGTG_nn'
Cuts at: 0 1479 1504 1731 1995
Size: 1479 25 227 264
Fragments arranged by size:
1479 264 227 25
DraIII CAC_nnn'GTG
Cuts at: 0 1021 1995
Size: 1021 974
EaeI y'GGCC_r
Cuts at: 0 94 1995
Size: 94 1901
EarI CTCTTCn'nnn_
Cuts at: 0 300 331 775 1838 1925 1995
Size: 300 31 444 1063 87 70
Fragments arranged by size:
1063 444 300 87 70 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 741 1845 1995
Size: 741 1104 150
Fragments arranged by size:
1104 741 150
EcoICRI GAG'CTC
Cuts at: 0 201 1995
Size: 201 1794
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 352 926 967 1412 1995
Size: 281 71 574 41 445 583
Fragments arranged by size:
583 574 445 281 71 41
EcoNI CCTnn'n_nnAGG
Cuts at: 0 526 1995
Size: 526 1469
EcoO109I rG'GnC_Cy
Cuts at: 0 1490 1995
Size: 1490 505
EcoRI G'AATT_C
Cuts at: 0 482 1995
Size: 482 1513
EcoRV GAT'ATC
Cuts at: 0 1209 1626 1995
Size: 1209 417 369
Fragments arranged by size:
1209 417 369
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 612 1212 1995
Size: 612 600 783
Fragments arranged by size:
783 612 600
FspI TGC'GCA
Cuts at: 0 1792 1995
Size: 1792 203
HaeII r_GCGC'y
Cuts at: 0 1138 1995
Size: 1138 857
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 6 38 256 271 288 303 1198 1230
Size: 6 32 218 15 17 15 895 32
Cuts at: 1230 1763 1795 1889 1906 1921 1938 1995
Size: 533 32 94 17 15 17 57
Fragments arranged by size:
895 533 218 94 57 32 32 32
17 17 17 15 15 15 6
HincII GTy'rAC
Cuts at: 0 231 1995
Size: 231 1764
HindIII A'AGCT_T
Cuts at: 0 154 806 1995
Size: 154 652 1189
Fragments arranged by size:
1189 652 154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 647 1995
Size: 647 1348
MslI CAynn'nnrTG
Cuts at: 0 1514 1995
Size: 1514 481
MspA1I CmG'CkG
Cuts at: 0 1125 1667 1748 1758 1995
Size: 1125 542 81 10 237
Fragments arranged by size:
1125 542 237 81 10
NaeI GCC'GGC
Cuts at: 0 94 1995
Size: 94 1901
NgoMIV G'CCGG_C
Cuts at: 0 92 1995
Size: 92 1903
NspI r_CATG'y
Cuts at: 0 795 1995
Size: 795 1200
PciI A'CATG_T
Cuts at: 0 791 1995
Size: 791 1204
PflMI CCAn_nnn'nTGG
Cuts at: 0 126 873 1667 1995
Size: 126 747 794 328
Fragments arranged by size:
794 747 328 126
PfoI T'CCnGG_A
Cuts at: 0 872 1995
Size: 872 1123
PpuMI rG'GwC_Cy
Cuts at: 0 1490 1995
Size: 1490 505
PstI C_TGCA'G
Cuts at: 0 1415 1753 1995
Size: 1415 338 242
Fragments arranged by size:
1415 338 242
PvuII CAG'CTG
Cuts at: 0 1667 1748 1758 1995
Size: 1667 81 10 237
Fragments arranged by size:
1667 237 81 10
SacI G_AGCT'C
Cuts at: 0 203 1995
Size: 203 1792
SapI GCTCTTCn'nnn_
Cuts at: 0 331 1925 1995
Size: 331 1594 70
Fragments arranged by size:
1594 331 70
SfcI C'TryA_G
Cuts at: 0 1411 1743 1749 1995
Size: 1411 332 6 246
Fragments arranged by size:
1411 332 246 6
SmlI C'TyrA_G
Cuts at: 0 931 1186 1606 1995
Size: 931 255 420 389
Fragments arranged by size:
931 420 389 255
StuI AGG'CCT
Cuts at: 0 1425 1995
Size: 1425 570
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1446 1995
Size: 1446 549
TatI w'GTAC_w
Cuts at: 0 315 1995
Size: 315 1680
Tth111I GACn'n_nGTC
Cuts at: 0 1777 1995
Size: 1777 218
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 13 1891 1995
Size: 13 1878 104
Fragments arranged by size:
1878 104 13
XmnI GAAnn'nnTTC
Cuts at: 0 340 1995
Size: 340 1655
Enzymes that do cut:
AarI AcuI AflII AflIII AlfI AlwNI ApaLI ApoI
AseI AvaI BanI BanII BbsI BbvCI BglI Bme1580I
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrFI BstAPI BstXI BstYI BtgI BtgZI BtsI
DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
MmeI MslI MspA1I NaeI NgoMIV NspI PciI PflMI
PfoI PpuMI PstI PvuII SacI SapI SfcI SmlI
StuI TaqII TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AhdI AleI
AloI ApaI AscI AsiSI AvrII BaeI BamHI BbeI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI
BmtI BsaAI BsaBI BsaWI BsiEI BsiWI BspEI BsrDI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI
CspCI DraI DrdI EagI FseI FspAI HpaI KasI
KpnI MfeI MluI MscI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StyI
SwaI TstI XbaI XhoI XmaI ZraI