Restriction map for
pFN21AA1694
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# NO CUT ENZYMES ( 88 enzymes) #
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Acc65I AccI AclI AflII AgeI
Aor51HI AscI AseI AsnI Asp718I
AspI AviII AvrII BamHI BbvCI
BcgI BciVI BclI BfaI BfrI
BlnI BsaBI BsiWI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HpaI KpnI MaeI
MamI MluI MseI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SalI
SfiI SfuI SgrAI SnaBI SpeI
SspBI SspI StuI SwaI Tru9I
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 2296 GACGTC GACGT^C
AflIII 77 ACGTGT A^C[GA][CT]GT
AhdI 1135 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1639 GTGCAC G^TGCAC
AocI 240 CCTGAGG CC^T[ACGT]AGG
ApaLI 1639 GTGCAC G^TGCAC
AspEI 1135 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1418 TGGCCA TGG^CCA
BbeI 106 GGCGCC GGCGC^C
BbrPI 1207 CACGTG CAC^GTG
BseRI 49 GAGGAG GAGGAG(10/8)
BsmFI 1709 GTCCC GGGAC(10/14)
BsmI 369 GAATGC GAATGC(1/-1)
BspMI 440 ACCTGC ACCTGC(4/8)
BsrBI 754 GAGCGG CCGCTC(-3/-3)
BsrDI 468 GCAATG GCAATG(2/0)
BstAPI 740 GCAAGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 240 CCTGAGG CC^T[ACGT]AGG
DrdI 175 GACATTCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1135 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 240 CCTGAGG CC^T[ACGT]AGG
EcoRI 730 GAATTC G^AATTC
HindIII 2272 AAGCTT A^AGCTT
KasI 106 GGCGCC G^GCGCC
KspI 202 CCGCGG CCGC^GG
MfeI 338 CAATTG C^AATTG
MluNI 1418 TGGCCA TGG^CCA
MscI 1418 TGGCCA TGG^CCA
MunI 338 CAATTG C^AATTG
NarI 106 GGCGCC GG^CGCC
PflMI 1740 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1207 CACGTG CAC^GTG
PmlI 1207 CACGTG CAC^GTG
PshAI 2160 GACCCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 202 CCGCGG CCGC^GG
SapI 127 GCTCTTC GCTCTTC(1/4)
SbfI 579 CCTGCAGG CCTGCA^GG
ScaI 380 AGTACT AGT^ACT
SfoI 106 GGCGCC GGC^GCC
SmaI 962 CCCGGG CCC^GGG
Sse8387I 579 CCTGCAGG CCTGCA^GG
Van91I 1740 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 962 CCCGGG C^CCGGG
XmaI 962 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccIII 545 TCCGGA T^CCGGA
1064 TCCGGA
ApaI 1335 GGGCCC GGGCC^C
1909 GGGCCC
Asp700I 343 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
912 GAAGAAGTTC
AvaI 18 CTCGGG C^[CT]CG[GA]G
962 CCCGGG
BanI 106 GGCGCC G^G[CT][GA]CC
197 GGCACC
BbsI 343 GAAGAC GAAGAC(2/6)
1513 GAAGAC
BglI 1234 GCCAAGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1911 GCCCACCAGGC
BglII 720 AGATCT A^GATCT
2259 AGATCT
BlpI 1545 GCTGAGC GC^T[ACGT]AGC
1965 GCTGAGC
Bpu1102I 1545 GCTGAGC GC^T[ACGT]AGC
1965 GCTGAGC
BpuAI 343 GAAGAC GAAGAC(2/6)
1513 GAAGAC
BseAI 545 TCCGGA T^CCGGA
1064 TCCGGA
BsgI 1120 CTGCAC GTGCAG(16/14)
799 GTGCAG
BsiEI 1089 CGACCG CG[GA][CT]^CG
1347 CGACCG
BsoBI 18 CTCGGG C^[CT]CG[GA]G
962 CCCGGG
BspEI 545 TCCGGA T^CCGGA
1064 TCCGGA
BstXI 514 CCAAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
974 CCAACCTGGTGG
CelII 1545 GCTGAGC GC^T[ACGT]AGC
1965 GCTGAGC
MroI 545 TCCGGA T^CCGGA
1064 TCCGGA
NaeI 1239 GCCGGC GCC^GGC
1558 GCCGGC
NcoI 767 CCATGG C^CATGG
1745 CCATGG
NgoMIV 1239 GCCGGC G^CCGGC
1558 GCCGGC
PleI 589 GACTC GAGTC(4/5)
2205 GAGTC
PpuMI 965 GGGTCCT [GA]G^G[AT]CC[CT]
1707 GGGTCCC
SexAI 977 ACCTGGT A^CC[AT]GGT
1136 ACCTGGT
SfaNI 1767 GATGC GCATC(5/9)
1325 GCATC
SphI 929 GCATGC GCATG^C
1715 GCATGC
XcmI 56 CCAAGCCGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1127 CCAGCCCGGACCTGG
XmnI 343 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
912 GAAGAAGTTC
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# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BanII BbvI BcnI BmyI
BpmI BsaAI BsaHI BsaI BsaJI
BsaWI BsiHKAI BsiYI BslI BsmAI
BsmBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfcI SmlI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII