Restriction map for
pFN21AA1694
(Linear) (Six-base) MAPSORT of: KIAA1694.seq Check: 989 from: 1 to: 2319
KIAA1694.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AatII G_ACGT'C
Cuts at: 0 2300 2319
Size: 2300 19
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1075 1507 1906 2284 2319
Size: 1075 432 399 378 35
Fragments arranged by size:
1075 432 399 378 35
AflIII A'CryG_T
Cuts at: 0 77 2319
Size: 77 2242
AhdI GACnn_n'nnGTC
Cuts at: 0 1140 2319
Size: 1140 1179
AleI CACnn'nnGTG
Cuts at: 0 793 1837 2319
Size: 793 1044 482
Fragments arranged by size:
1044 793 482
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2065 2097 2319
Size: 2065 32 222
Fragments arranged by size:
2065 222 32
AlwNI CAG_nnn'CTG
Cuts at: 0 357 1948 2030 2145 2176 2319
Size: 357 1591 82 115 31 143
Fragments arranged by size:
1591 357 143 115 82 31
ApaI G_GGCC'C
Cuts at: 0 1339 1913 2319
Size: 1339 574 406
Fragments arranged by size:
1339 574 406
ApaLI G'TGCA_C
Cuts at: 0 1639 2319
Size: 1639 680
ApoI r'AATT_y
Cuts at: 0 235 730 1675 2319
Size: 235 495 945 644
Fragments arranged by size:
945 644 495 235
AvaI C'yCGr_G
Cuts at: 0 18 962 2319
Size: 18 944 1357
Fragments arranged by size:
1357 944 18
BanI G'GyrC_C
Cuts at: 0 106 197 2319
Size: 106 91 2122
Fragments arranged by size:
2122 106 91
BanII G_rGCy'C
Cuts at: 0 129 890 1277 1339 1657 1913 1972 2197
Size: 129 761 387 62 318 256 59 225
Cuts at: 2197 2319
Size: 122
Fragments arranged by size:
761 387 318 256 225 129 122 62
59
BbeI G_GCGC'C
Cuts at: 0 110 2319
Size: 110 2209
BbsI GAAGACnn'nnnn_
Cuts at: 0 350 1520 2319
Size: 350 1170 799
Fragments arranged by size:
1170 799 350
BglI GCCn_nnn'nGGC
Cuts at: 0 1240 1917 2319
Size: 1240 677 402
Fragments arranged by size:
1240 677 402
BglII A'GATC_T
Cuts at: 0 720 2259 2319
Size: 720 1539 60
Fragments arranged by size:
1539 720 60
BlpI GC'TnA_GC
Cuts at: 0 1546 1966 2319
Size: 1546 420 353
Fragments arranged by size:
1546 420 353
Bme1580I G_kGCm'C
Cuts at: 0 116 841 950 1339 1643 1913 2319
Size: 116 725 109 389 304 270 406
Fragments arranged by size:
725 406 389 304 270 116 109
BmgBI CAC'GTC
Cuts at: 0 78 1839 2319
Size: 78 1761 480
Fragments arranged by size:
1761 480 78
BmrI ACTGGGnnnn_n'
Cuts at: 0 314 2319
Size: 314 2005
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1430 1551 2319
Size: 1430 121 768
Fragments arranged by size:
1430 768 121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1179 1425 1846 2011 2122 2319
Size: 1179 246 421 165 111 197
Fragments arranged by size:
1179 421 246 197 165 111
Bpu10I CC'TnA_GC
Cuts at: 0 2191 2319
Size: 2191 128
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 1159 1363 2047 2319
Size: 796 363 204 684 272
Fragments arranged by size:
796 684 363 272 204
BsaI GGTCTCn'nnnn_
Cuts at: 0 46 440 1401 2152 2319
Size: 46 394 961 751 167
Fragments arranged by size:
961 751 394 167 46
BsaAI yAC'GTr
Cuts at: 0 1209 1368 1494 2319
Size: 1209 159 126 825
Fragments arranged by size:
1209 825 159 126
BsaHI Gr'CG_yC
Cuts at: 0 107 289 2297 2319
Size: 107 182 2008 22
Fragments arranged by size:
2008 182 107 22
BsaWI w'CCGG_w
Cuts at: 0 545 1064 1091 2319
Size: 545 519 27 1228
Fragments arranged by size:
1228 545 519 27
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 310 340 1744 1774 2319
Size: 310 30 1404 30 545
Fragments arranged by size:
1404 545 310 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 2319
Size: 64 2255
BseYI C'CCAG_C
Cuts at: 0 66 248 521 1126 1332 1704 2319
Size: 66 182 273 605 206 372 615
Fragments arranged by size:
615 605 372 273 206 182 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 1105 2319
Size: 820 285 1214
Fragments arranged by size:
1214 820 285
BsiEI CG_ry'CG
Cuts at: 0 1092 1350 2319
Size: 1092 258 969
Fragments arranged by size:
1092 969 258
BsiHKAI G_wGCw'C
Cuts at: 0 890 1643 1834 1972 2042 2197 2319
Size: 890 753 191 138 70 155 122
Fragments arranged by size:
890 753 191 155 138 122 70
BsmI GAATG_Cn'
Cuts at: 0 375 2319
Size: 375 1944
BsmBI CGTCTCn'nnnn_
Cuts at: 0 624 1418 2090 2319
Size: 624 794 672 229
Fragments arranged by size:
794 672 624 229
Bsp1286I G_dGCh'C
Cuts at: 0 116 129 841 890 950 1277 1339 1643
Size: 116 13 712 49 60 327 62 304
Cuts at: 1643 1657 1834 1913 1972 2042 2197 2319
Size: 14 177 79 59 70 155 122
Fragments arranged by size:
712 327 304 177 155 122 116 79
70 62 60 59 49 14 13
BspEI T'CCGG_A
Cuts at: 0 545 1064 2319
Size: 545 519 1255
Fragments arranged by size:
1255 545 519
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 449 2319
Size: 449 1870
BsrBI CCG'CTC
Cuts at: 0 756 2319
Size: 756 1563
BsrDI GCAATG_nn'
Cuts at: 0 475 2319
Size: 475 1844
BsrFI r'CCGG_y
Cuts at: 0 1239 1558 1871 2319
Size: 1239 319 313 448
Fragments arranged by size:
1239 448 319 313
BstAPI GCAn_nnn'nTGC
Cuts at: 0 746 2319
Size: 746 1573
BstXI CCAn_nnnn'nTGG
Cuts at: 0 521 981 2319
Size: 521 460 1338
Fragments arranged by size:
1338 521 460
BstYI r'GATC_y
Cuts at: 0 221 542 720 1202 1754 2259 2319
Size: 221 321 178 482 552 505 60
Fragments arranged by size:
552 505 482 321 221 178 60
Bsu36I CC'TnA_GG
Cuts at: 0 241 2319
Size: 241 2078
BtgI C'CryG_G
Cuts at: 0 202 767 794 1745 2319
Size: 202 565 27 951 574
Fragments arranged by size:
951 574 565 202 27
BtsI GCAGTG_nn'
Cuts at: 0 744 948 1229 1924 2319
Size: 744 204 281 695 395
Fragments arranged by size:
744 695 395 281 204
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2073 2108 2319
Size: 2073 35 211
Fragments arranged by size:
2073 211 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 181 2319
Size: 181 2138
EaeI y'GGCC_r
Cuts at: 0 272 983 1250 1418 2319
Size: 272 711 267 168 901
Fragments arranged by size:
901 711 272 267 168
EarI CTCTTCn'nnn_
Cuts at: 0 134 1013 2197 2319
Size: 134 879 1184 122
Fragments arranged by size:
1184 879 134 122
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 282 2319
Size: 282 2037
EcoICRI GAG'CTC
Cuts at: 0 888 1970 2195 2319
Size: 888 1082 225 124
Fragments arranged by size:
1082 888 225 124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1075 1179 1425 1507 1846 1906 2011 2122
Size: 1075 104 246 82 339 60 105 111
Cuts at: 2122 2284 2319
Size: 162 35
Fragments arranged by size:
1075 339 246 162 111 105 104 82
60 35
EcoO109I rG'GnC_Cy
Cuts at: 0 603 966 1336 1708 2319
Size: 603 363 370 372 611
Fragments arranged by size:
611 603 372 370 363
EcoRI G'AATT_C
Cuts at: 0 730 2319
Size: 730 1589
HaeII r_GCGC'y
Cuts at: 0 110 249 1246 2319
Size: 110 139 997 1073
Fragments arranged by size:
1073 997 139 110
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1126 1158 2319
Size: 1126 32 1161
Fragments arranged by size:
1161 1126 32
HindIII A'AGCT_T
Cuts at: 0 2272 2319
Size: 2272 47
KasI G'GCGC_C
Cuts at: 0 106 2319
Size: 106 2213
MfeI C'AATT_G
Cuts at: 0 338 2319
Size: 338 1981
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2099 2319
Size: 2099 220
MscI TGG'CCA
Cuts at: 0 1420 2319
Size: 1420 899
MslI CAynn'nnrTG
Cuts at: 0 100 676 781 793 1648 1837 2319
Size: 100 576 105 12 855 189 482
Fragments arranged by size:
855 576 482 189 105 100 12
MspA1I CmG'CkG
Cuts at: 0 63 204 354 521 1863 1878 2070 2245
Size: 63 141 150 167 1342 15 192 175
Cuts at: 2245 2319
Size: 74
Fragments arranged by size:
1342 192 175 167 150 141 74 63
15
NaeI GCC'GGC
Cuts at: 0 1241 1560 2319
Size: 1241 319 759
Fragments arranged by size:
1241 759 319
NarI GG'CG_CC
Cuts at: 0 107 2319
Size: 107 2212
NcoI C'CATG_G
Cuts at: 0 767 1745 2319
Size: 767 978 574
Fragments arranged by size:
978 767 574
NgoMIV G'CCGG_C
Cuts at: 0 1239 1558 2319
Size: 1239 319 761
Fragments arranged by size:
1239 761 319
NspI r_CATG'y
Cuts at: 0 933 1719 1854 2319
Size: 933 786 135 465
Fragments arranged by size:
933 786 465 135
PflMI CCAn_nnn'nTGG
Cuts at: 0 1746 2319
Size: 1746 573
PmlI CAC'GTG
Cuts at: 0 1209 2319
Size: 1209 1110
PpuMI rG'GwC_Cy
Cuts at: 0 966 1708 2319
Size: 966 742 611
Fragments arranged by size:
966 742 611
PshAI GACnn'nnGTC
Cuts at: 0 2164 2319
Size: 2164 155
PspOMI G'GGCC_C
Cuts at: 0 1335 1909 2319
Size: 1335 574 410
Fragments arranged by size:
1335 574 410
PstI C_TGCA'G
Cuts at: 0 167 425 584 751 1315 1811 2215 2319
Size: 167 258 159 167 564 496 404 104
Fragments arranged by size:
564 496 404 258 167 167 159 104
PvuII CAG'CTG
Cuts at: 0 354 1863 2070 2245 2319
Size: 354 1509 207 175 74
Fragments arranged by size:
1509 354 207 175 74
SacI G_AGCT'C
Cuts at: 0 890 1972 2197 2319
Size: 890 1082 225 122
Fragments arranged by size:
1082 890 225 122
SacII CC_GC'GG
Cuts at: 0 205 2319
Size: 205 2114
SanDI GG'GwC_CC
Cuts at: 0 1708 2319
Size: 1708 611
SapI GCTCTTCn'nnn_
Cuts at: 0 134 2319
Size: 134 2185
SbfI CC_TGCA'GG
Cuts at: 0 584 2319
Size: 584 1735
ScaI AGT'ACT
Cuts at: 0 382 2319
Size: 382 1937
SexAI A'CCwGG_T
Cuts at: 0 977 1136 2319
Size: 977 159 1183
Fragments arranged by size:
1183 977 159
SfcI C'TryA_G
Cuts at: 0 163 330 421 580 747 1311 1584 1807
Size: 163 167 91 159 167 564 273 223
Cuts at: 1807 2211 2319
Size: 404 108
Fragments arranged by size:
564 404 273 223 167 167 163 159
108 91
SfoI GGC'GCC
Cuts at: 0 108 2319
Size: 108 2211
SmaI CCC'GGG
Cuts at: 0 964 2319
Size: 964 1355
SmlI C'TyrA_G
Cuts at: 0 811 1174 1378 2062 2319
Size: 811 363 204 684 257
Fragments arranged by size:
811 684 363 257 204
SphI G_CATG'C
Cuts at: 0 933 1719 2319
Size: 933 786 600
Fragments arranged by size:
933 786 600
StyI C'CwwG_G
Cuts at: 0 767 1478 1745 2319
Size: 767 711 267 574
Fragments arranged by size:
767 711 574 267
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 670 725 1816 2319
Size: 670 55 1091 503
Fragments arranged by size:
1091 670 503 55
TatI w'GTAC_w
Cuts at: 0 380 866 2319
Size: 380 486 1453
Fragments arranged by size:
1453 486 380
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1744 1776 2319
Size: 1744 32 543
Fragments arranged by size:
1744 543 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 63 1134 2319
Size: 63 1071 1185
Fragments arranged by size:
1185 1071 63
XmaI C'CCGG_G
Cuts at: 0 962 2319
Size: 962 1357
XmnI GAAnn'nnTTC
Cuts at: 0 347 916 2319
Size: 347 569 1403
Fragments arranged by size:
1403 569 347
ZraI GAC'GTC
Cuts at: 0 2298 2319
Size: 2298 21
Enzymes that do cut:
AatII AcuI AflIII AhdI AleI AloI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbeI BbsI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI
BtsI CspCI DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI HaeII Hin4I HindIII KasI MfeI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PflMI PmlI PpuMI PshAI PspOMI PstI PvuII SacI
SacII SanDI SapI SbfI ScaI SexAI SfcI SfoI
SmaI SmlI SphI StyI TaqII TatI TstI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AlfI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI
BciVI BclI BfrBI BmtI BsaBI BsiWI BspHI BsrGI
BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI DraI
DraIII EagI EcoNI EcoRV FalI FseI FspI FspAI
HincII HpaI KpnI MluI NdeI NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PpiI PsiI
PspXI PsrI PvuI RsrII SalI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI
XhoI