(Circular) (Six-base) MAPSORT of: pFN21AA1667.seq Check: 4862 from: 1 to: 6829 pFN21AA1667 6829 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3853 4036 3853 Size: 183 6646 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4639 278 Size: 53 83 186 936 3103 2468 Fragments arranged by size: 3103 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4172 1937 Size: 2235 4594 Acc65I G'GTAC_C Cuts at: 4150 4150 Size: 6829 AclI AA'CG_TT Cuts at: 1563 4956 5329 1563 Size: 3393 373 3063 Fragments arranged by size: 3393 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2264 2546 4057 4127 4892 6276 6544 1632 Size: 632 282 1511 70 765 1384 268 1917 Fragments arranged by size: 1917 1511 1384 765 632 282 268 70 AfeI AGC'GCT Cuts at: 2558 2558 Size: 6829 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6632 AflIII A'CryG_T Cuts at: 1203 3175 5728 6705 1203 Size: 1972 2553 977 1327 Fragments arranged by size: 2553 1972 1327 977 AgeI A'CCGG_T Cuts at: 5660 5660 Size: 6829 AhdI GACnn_n'nnGTC Cuts at: 2603 5558 2603 Size: 2955 3874 AleI CACnn'nnGTG Cuts at: 1166 3974 1166 Size: 2808 4021 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3051 3622 1184 Size: 1867 571 4391 Fragments arranged by size: 4391 1867 571 AlwNI CAG_nnn'CTG Cuts at: 2140 3515 4021 4102 4572 6144 2140 Size: 1375 506 81 470 1572 2825 Fragments arranged by size: 2825 1572 1375 506 470 81 ApaLI G'TGCA_C Cuts at: 4886 6042 4886 Size: 1156 5673 ApoI r'AATT_y Cuts at: 1493 2439 3920 4138 4364 4516 6625 1493 Size: 946 1481 218 226 152 2109 1697 Fragments arranged by size: 2109 1697 1481 946 226 218 152 AseI AT'TA_AT Cuts at: 160 1032 5383 160 Size: 872 4351 1606 Fragments arranged by size: 4351 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6829 AvaI C'yCGr_G Cuts at: 1870 1943 4154 1870 Size: 73 2211 4545 Fragments arranged by size: 4545 2211 73 BamHI G'GATC_C Cuts at: 4159 4526 4159 Size: 367 6462 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4024 4067 4150 5605 618 Size: 325 217 627 2237 43 83 1455 1842 Fragments arranged by size: 2237 1842 1455 627 325 217 83 43 BanII G_rGCy'C Cuts at: 1391 2304 2514 3202 3322 3618 4043 4148 1391 Size: 913 210 688 120 296 425 105 4072 Fragments arranged by size: 4072 913 688 425 296 210 120 105 BbeI G_GCGC'C Cuts at: 4071 4071 Size: 6829 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3342 928 Size: 971 1443 4415 Fragments arranged by size: 4415 1443 971 BbvCI CC'TCA_GC Cuts at: 3059 3675 3059 Size: 616 6213 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3598 3632 5020 5054 1903 Size: 34 1661 34 1388 34 3678 Fragments arranged by size: 3678 1661 1388 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4721 5931 4721 Size: 1210 5619 BclI T'GATC_A Cuts at: 1548 1548 Size: 6829 BfrBI ATG'CAT Cuts at: 3913 3913 Size: 6829 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3971 4083 5440 Size: 107 122 71 631 605 2299 112 1357 Cuts at: 5440 136 Size: 1525 Fragments arranged by size: 2299 1525 1357 631 605 122 112 107 71 BglII A'GATC_T Cuts at: 6824 6824 Size: 6829 BlpI GC'TnA_GC Cuts at: 3538 4246 3538 Size: 708 6121 Bme1580I G_kGCm'C Cuts at: 1790 4890 6046 1790 Size: 3100 1156 2573 Fragments arranged by size: 3100 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6829 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4560 5518 442 Size: 416 53 83 399 24 3143 958 1753 Fragments arranged by size: 3143 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6829 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3808 3808 Size: 6829 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2199 2805 2816 3023 3214 3568 3878 5489 Size: 960 606 11 207 191 354 310 1611 Cuts at: 5489 1239 Size: 2579 Fragments arranged by size: 2579 1611 960 606 354 310 207 191 11 Bpu10I CC'TnA_GC Cuts at: 3059 3135 3675 3059 Size: 76 540 6213 Fragments arranged by size: 6213 540 76 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4954 5819 6117 4954 Size: 865 298 5666 Fragments arranged by size: 5666 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2814 5492 882 Size: 617 1315 2678 2219 Fragments arranged by size: 2678 2219 1315 617 BsaAI yAC'GTr Cuts at: 493 1204 4620 493 Size: 711 3416 2702 Fragments arranged by size: 3416 2702 711 BsaBI GATnn'nnATC Cuts at: 6823 6823 Size: 6829 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4068 4636 5018 5653 Size: 53 83 186 936 2535 568 382 635 Cuts at: 5653 275 Size: 1451 Fragments arranged by size: 2535 1451 936 635 568 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5262 5660 5934 6081 6492 5262 Size: 398 274 147 411 5599 Fragments arranged by size: 5599 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2501 2531 1178 Size: 30 165 30 1098 30 5476 Fragments arranged by size: 5476 1098 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2205 3055 3692 1186 Size: 1019 850 637 4323 Fragments arranged by size: 4323 1019 850 637 BseYI C'CCAG_C Cuts at: 2284 2847 3309 3688 3927 6032 6528 2284 Size: 563 462 379 239 2105 496 2585 Fragments arranged by size: 2585 2105 563 496 462 379 239 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3011 1163 Size: 573 1275 4981 Fragments arranged by size: 4981 1275 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3829 4279 5040 6068 1540 Size: 275 186 1828 450 761 1028 2301 Fragments arranged by size: 2301 1828 1028 761 450 275 186 BsiHKAI G_wGCw'C Cuts at: 2304 2414 2873 4043 4890 4975 6046 2304 Size: 110 459 1170 847 85 1071 3087 Fragments arranged by size: 3087 1170 1071 847 459 110 85 BsmI GAATG_Cn' Cuts at: 2538 3530 3831 4340 4433 2538 Size: 992 301 509 93 4934 Fragments arranged by size: 4934 992 509 301 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3761 3761 Size: 6829 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2304 2414 2514 2873 3202 3322 3618 Size: 399 514 110 100 359 329 120 296 Cuts at: 3618 4043 4148 4890 4975 6046 1391 Size: 425 105 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 514 425 399 359 329 296 120 110 105 100 85 BspEI T'CCGG_A Cuts at: 6492 6492 Size: 6829 BspHI T'CATG_A Cuts at: 4718 6448 4718 Size: 1730 5099 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2558 3319 3853 3864 4036 4184 844 Size: 1714 761 534 11 172 148 3489 Fragments arranged by size: 3489 1714 761 534 172 148 11 BsrBI CCG'CTC Cuts at: 1394 1823 2714 4716 1394 Size: 429 891 2002 3507 Fragments arranged by size: 3507 2002 891 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2717 5324 5498 65 Size: 1183 1469 2607 174 1396 Fragments arranged by size: 2607 1469 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5473 5660 1700 Size: 110 3663 187 2869 Fragments arranged by size: 3663 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6829 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6829 BssSI C'ACGA_G Cuts at: 2041 2780 3617 3796 4889 5901 2041 Size: 739 837 179 1093 1012 2969 Fragments arranged by size: 2969 1093 1012 837 739 179 BstAPI GCAn_nnn'nTGC Cuts at: 794 4102 794 Size: 3308 3521 BstBI TT'CG_AA Cuts at: 5633 5633 Size: 6829 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6829 BstXI CCAn_nnnn'nTGG Cuts at: 3238 3238 Size: 6829 BstYI r'GATC_y Cuts at: 1972 2932 3427 4159 4526 4911 4928 6380 6466 Size: 960 495 732 367 385 17 1452 86 Cuts at: 6466 6478 6824 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 960 732 495 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2664 3154 3255 4059 2664 Size: 490 101 804 5434 Fragments arranged by size: 5434 804 490 101 BtgI C'CryG_G Cuts at: 513 1065 2004 2913 3456 3969 513 Size: 552 939 909 543 513 3373 Fragments arranged by size: 3373 939 909 552 543 513 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6090 Fragments arranged by size: 6090 683 56 BtsI GCAGTG_nn' Cuts at: 3640 4345 5139 5159 3640 Size: 705 794 20 5310 Fragments arranged by size: 5310 794 705 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6829 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6794 DraI TTT'AAA Cuts at: 4133 4133 Size: 6829 DraIII CAC_nnn'GTG Cuts at: 2418 4117 2418 Size: 1699 5130 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5836 809 Size: 507 4520 1802 Fragments arranged by size: 4520 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2899 2992 Size: 54 1425 50 275 18 177 892 93 Cuts at: 2992 3346 3867 4276 5165 6794 8 Size: 354 521 409 889 1629 43 Fragments arranged by size: 1629 1425 892 889 521 409 354 275 177 93 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4276 1537 Size: 275 2464 4090 Fragments arranged by size: 4090 2464 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4759 1353 Size: 3406 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5416 5790 5936 387 Size: 1064 138 3827 374 146 1280 Fragments arranged by size: 3827 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2302 4041 2302 Size: 1739 5090 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2199 2264 2546 2805 2816 3023 3214 Size: 393 567 65 282 259 11 207 191 Cuts at: 3214 3568 3878 4057 4127 4892 5489 6276 6544 Size: 354 310 179 70 765 597 787 268 Cuts at: 6544 1239 Size: 1524 Fragments arranged by size: 1524 787 765 597 567 393 354 310 282 268 259 207 191 179 70 65 11 EcoNI CCTnn'n_nnAGG Cuts at: 2685 3074 3476 3860 2685 Size: 389 402 384 5654 Fragments arranged by size: 5654 402 389 384 EcoO109I rG'GnC_Cy Cuts at: 2641 3396 3405 2641 Size: 755 9 6065 Fragments arranged by size: 6065 755 9 EcoRI G'AATT_C Cuts at: 4138 4516 4138 Size: 378 6451 FspI TGC'GCA Cuts at: 5335 5335 Size: 6829 HaeII r_GCGC'y Cuts at: 1678 2560 3228 4071 5976 1678 Size: 882 668 843 1905 2531 Fragments arranged by size: 2531 1905 882 843 668 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2018 2050 2589 2621 2794 Size: 32 135 32 446 32 539 32 173 Cuts at: 2794 2826 2924 2956 3613 3645 5470 5502 5544 Size: 32 98 32 657 32 1825 32 42 Cuts at: 5544 5576 1373 Size: 32 2626 Fragments arranged by size: 2626 1825 657 539 446 173 135 98 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4173 4419 669 Size: 988 281 2235 246 3079 Fragments arranged by size: 3079 2235 988 281 246 HindIII A'AGCT_T Cuts at: 748 2952 3449 748 Size: 2204 497 4128 Fragments arranged by size: 4128 2204 497 HpaI GTT'AAC Cuts at: 4419 4419 Size: 6829 KasI G'GCGC_C Cuts at: 4067 4067 Size: 6829 KpnI G_GTAC'C Cuts at: 4154 4154 Size: 6829 MfeI C'AATT_G Cuts at: 4428 4428 Size: 6829 MluI A'CGCG_T Cuts at: 6705 6705 Size: 6829 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2123 2323 5943 6127 1305 Size: 818 200 3620 184 2007 Fragments arranged by size: 3620 2007 818 200 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2901 2994 3348 3869 Size: 54 1425 343 177 892 93 354 521 Cuts at: 3869 10 Size: 2970 Fragments arranged by size: 2970 1425 892 521 354 343 177 93 54 MslI CAynn'nnrTG Cuts at: 518 1166 3974 4787 5146 5305 518 Size: 648 2808 813 359 159 2042 Fragments arranged by size: 2808 2042 813 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2190 2316 3685 3931 4021 4245 4922 6070 Size: 584 126 1369 246 90 224 677 1148 Cuts at: 6070 6315 1606 Size: 245 2120 Fragments arranged by size: 2120 1369 1148 677 584 246 245 224 126 90 NarI GG'CG_CC Cuts at: 4068 4068 Size: 6829 NcoI C'CATG_G Cuts at: 513 1065 2004 2913 513 Size: 552 939 909 4429 Fragments arranged by size: 4429 939 909 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6829 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6829 NotI GC'GGCC_GC Cuts at: 4276 4276 Size: 6829 NsiI A_TGCA'T Cuts at: 3915 3915 Size: 6829 NspI r_CATG'y Cuts at: 1132 1229 3179 4187 5732 1132 Size: 97 1950 1008 1545 2229 Fragments arranged by size: 2229 1950 1545 1008 97 PasI CC'CwG_GG Cuts at: 1348 3070 3777 1348 Size: 1722 707 4400 Fragments arranged by size: 4400 1722 707 PciI A'CATG_T Cuts at: 3175 5728 3175 Size: 2553 4276 PflMI CCAn_nnn'nTGG Cuts at: 1108 2864 2919 3001 4021 1108 Size: 1756 55 82 1020 3916 Fragments arranged by size: 3916 1756 1020 82 55 PfoI T'CCnGG_A Cuts at: 2832 3430 3999 2832 Size: 598 569 5662 Fragments arranged by size: 5662 598 569 PmeI GTTT'AAAC Cuts at: 4133 4133 Size: 6829 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2647 2679 4898 4930 6411 6443 2647 Size: 32 2219 32 1481 32 3033 Fragments arranged by size: 3033 2219 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2641 3396 2641 Size: 755 6074 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6829 PsiI TTA'TAA Cuts at: 4399 4399 Size: 6829 PstI C_TGCA'G Cuts at: 830 2547 3571 4033 4181 830 Size: 1717 1024 462 148 3478 Fragments arranged by size: 3478 1717 1024 462 148 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6829 PvuII CAG'CTG Cuts at: 2316 3931 4021 2316 Size: 1615 90 5124 Fragments arranged by size: 5124 1615 90 SacI G_AGCT'C Cuts at: 2304 4043 2304 Size: 1739 5090 SalI G'TCGA_C Cuts at: 1936 4171 1936 Size: 2235 4594 SbfI CC_TGCA'GG Cuts at: 4181 4181 Size: 6829 ScaI AGT'ACT Cuts at: 5077 6789 5077 Size: 1712 5117 SfcI C'TryA_G Cuts at: 826 1045 2543 3567 4029 4177 5312 5993 6184 Size: 219 1498 1024 462 148 1135 681 191 Cuts at: 6184 826 Size: 1471 Fragments arranged by size: 1498 1471 1135 1024 681 462 219 191 148 SfoI GGC'GCC Cuts at: 4069 4069 Size: 6829 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6829 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6829 SmaI CCC'GGG Cuts at: 1872 4156 1872 Size: 2284 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4933 5834 6096 820 Size: 197 926 2990 901 262 1553 Fragments arranged by size: 2990 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4620 493 Size: 4127 2702 SpeI A'CTAG_T Cuts at: 152 3082 152 Size: 2930 3899 SphI G_CATG'C Cuts at: 1132 4187 1132 Size: 3055 3774 SspI AAT'ATT Cuts at: 5 52 4753 5 Size: 47 4701 2081 Fragments arranged by size: 4701 2081 47 StuI AGG'CCT Cuts at: 1517 3471 1517 Size: 1954 4875 StyI C'CwwG_G Cuts at: 513 1065 2004 2076 2637 2913 4268 513 Size: 552 939 72 561 276 1355 3074 Fragments arranged by size: 3074 1355 939 561 552 276 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2269 2627 4856 4873 5026 5211 5656 Size: 1255 689 358 2229 17 153 185 445 Cuts at: 5656 325 Size: 1498 Fragments arranged by size: 2229 1498 1255 689 445 358 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5075 6787 96 Size: 274 80 33 51 1443 3098 1712 138 Fragments arranged by size: 3098 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2917 2949 6789 6821 2917 Size: 32 3840 32 2925 Fragments arranged by size: 3840 2925 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2806 1316 Size: 1490 5339 XbaI T'CTAG_A Cuts at: 4165 4165 Size: 6829 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2861 2902 3512 2861 Size: 41 610 6178 Fragments arranged by size: 6178 610 41 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6829 XmaI C'CCGG_G Cuts at: 1870 4154 1870 Size: 2284 4545 XmnI GAAnn'nnTTC Cuts at: 4958 4958 Size: 6829 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4637 276 Size: 53 83 186 936 3103 2468 Fragments arranged by size: 3103 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BsiWI BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI