Restriction map for
pFN21AA1646
##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
Acc65I AclI AfaI AflII AgeI
AhdI Alw44I AocI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BbrPI BbvCI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspHI BsrBI BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
KspI MaeI MfeI MluI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacI SacII SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Van91I XbaI XcmI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 378 GACGTC GACGT^C
AccI 649 GTCGAC GT^[AC][GT]AC
AcsI 1369 AAATTC [GA]^AATT[CT]
AflIII 1184 ACATGT A^C[GA][CT]GT
AlwI 1015 GGATC GGATC(4/5)
ApaI 115 GGGCCC GGGCC^C
ApoI 1369 AAATTC [GA]^AATT[CT]
AspHI 272 GAGCAC G[AT]GC[AT]^C
AspI 981 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 127 GGCGCC GGCGC^C
BbsI 1248 GTCTTC GAAGAC(2/6)
BcgI 760 GCAGAAACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1208 GCCCCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1248 GTCTTC GAAGAC(2/6)
BsaBI 696 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 272 GAGCAC G[AT]GC[AT]^C
BspLU11I 1184 ACATGT A^CATGT
BspMI 300 GCAGGT ACCTGC(4/8)
BsrFI 124 GCCGGC [GA]^CCGG[CT]
BssSI 1571 CACGAG CACGAG(-5/-1)
BstYI 1015 GGATCT [GA]^GATC[CT]
Cfr10I 124 GCCGGC [GA]^CCGG[CT]
DraI 945 TTTAAA TTT^AAA
DraIII 1006 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1582 GAAGAG CTCTTC(1/4)
EcoNI 1413 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI 127 GGCGCC G^GCGCC
Ksp632I 1582 GAAGAG CTCTTC(1/4)
MamI 696 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1015 GGATCT [GA]^GATC[CT]
NaeI 124 GCCGGC GCC^GGC
NarI 127 GGCGCC GG^CGCC
NcoI 803 CCATGG C^CATGG
NgoMIV 124 GCCGGC G^CCGGC
PflFI 981 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1511 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 346 CTGCAG CTGCA^G
SalI 649 GTCGAC G^TCGAC
SfcI 346 CTGCAG C^T[GA][CT]AG
SfoI 127 GGCGCC GGC^GCC
SmlI 1414 CTCAAG C^T[CT][GA]AG
Tth111I 981 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1015 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 692 TCCGGA T^CCGGA
1268 TCCGGA
AcyI 127 GGCGCC G[GA]^CG[CT]C
378 GACGTC
Aor51HI 56 AGCGCT AGC^GCT
1433 AGCGCT
AvaI 111 CCCGGG C^[CT]CG[GA]G
665 CCCGGG
BalI 800 TGGCCA TGG^CCA
1166 TGGCCA
BsaHI 127 GGCGCC G[GA]^CG[CT]C
378 GACGTC
BsaWI 692 TCCGGA [AT]^CCGG[AT]
1268 TCCGGA
BseAI 692 TCCGGA T^CCGGA
1268 TCCGGA
BsmBI 1479 CGTCTC CGTCTC(1/5)
1241 GAGACG
BsoBI 111 CCCGGG C^[CT]CG[GA]G
665 CCCGGG
BspEI 692 TCCGGA T^CCGGA
1268 TCCGGA
BstAPI 30 GCAATCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
348 GCAGACCCTGC
DpnII 168 GATC ^GATC
1016 GATC
DrdI 565 GACGGCATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1504 GACGGGGAGGTC
DsaI 743 CCGTGG C^C[GA][CT]GG
803 CCATGG
EaeI 800 TGGCCA [CT]^GGCC[GA]
1166 TGGCCA
Eco47III 56 AGCGCT AGC^GCT
1433 AGCGCT
Hin1I 127 GGCGCC G[GA]^CG[CT]C
378 GACGTC
HincII 649 GTCGAC GT[CT]^[GA]AC
717 GTCAAC
HindII 649 GTCGAC GT[CT]^[GA]AC
717 GTCAAC
MboI 168 GATC ^GATC
1016 GATC
MluNI 800 TGGCCA TGG^CCA
1166 TGGCCA
MroI 692 TCCGGA T^CCGGA
1268 TCCGGA
MscI 800 TGGCCA TGG^CCA
1166 TGGCCA
MseI 548 TTAA T^TAA
946 TTAA
NdeII 168 GATC ^GATC
1016 GATC
NspI 513 ACATGC [GA]CATG^[CT]
1184 ACATGT
Sau3AI 168 GATC ^GATC
1016 GATC
SmaI 111 CCCGGG CCC^GGG
665 CCCGGG
TfiI 701 GAATC G^A[AT]TC
1587 GAATC
Tru9I 548 TTAA T^TAA
946 TTAA
Tsp45I 304 GTGAC ^GT[GC]AC
335 GTCAC
XmaCI 111 CCCGGG C^CCGGG
665 CCCGGG
XmaI 111 CCCGGG C^CCGGG
665 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AluI AlwNI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PleI PvuII
Sau96I ScrFI SfaNI StyI TaqI
TseI Tsp509I TspRI TthHB8I