(Circular) (Six-base) MAPSORT of: pFN21AA1646.seq Check: 5379 from: 1 to: 6316 pFN21AA1646 6316 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2296 2296 Size: 6316 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2387 4126 278 Size: 53 83 186 936 851 1739 2468 Fragments arranged by size: 2468 1739 936 851 186 83 53 AccI GT'mk_AC Cuts at: 1937 2655 3659 1937 Size: 718 1004 4594 Fragments arranged by size: 4594 1004 718 Acc65I G'GTAC_C Cuts at: 3637 3637 Size: 6316 AclI AA'CG_TT Cuts at: 1563 4443 4816 1563 Size: 2880 373 3063 Fragments arranged by size: 3063 2880 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4379 5763 6031 1632 Size: 2747 1384 268 1917 Fragments arranged by size: 2747 1917 1384 268 AfeI AGC'GCT Cuts at: 2063 3440 2063 Size: 1377 4939 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6119 AflIII A'CryG_T Cuts at: 1203 3189 5215 6192 1203 Size: 1986 2026 977 1327 Fragments arranged by size: 2026 1986 1327 977 AgeI A'CCGG_T Cuts at: 5147 5147 Size: 6316 AhdI GACnn_n'nnGTC Cuts at: 5045 5045 Size: 6316 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6316 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3546 1184 Size: 2362 3954 AlwNI CAG_nnn'CTG Cuts at: 2359 3085 3498 4059 5631 2359 Size: 726 413 561 1572 3044 Fragments arranged by size: 3044 1572 726 561 413 ApaI G_GGCC'C Cuts at: 2124 2124 Size: 6316 ApaLI G'TGCA_C Cuts at: 4373 5529 4373 Size: 1156 5160 ApoI r'AATT_y Cuts at: 1493 3374 3625 3851 4003 6112 1493 Size: 1881 251 226 152 2109 1697 Fragments arranged by size: 2109 1881 1697 251 226 152 AseI AT'TA_AT Cuts at: 160 1032 4870 160 Size: 872 3838 1606 Fragments arranged by size: 3838 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6316 AvaI C'yCGr_G Cuts at: 1870 1943 2116 2670 3641 1870 Size: 73 173 554 971 4545 Fragments arranged by size: 4545 971 554 173 73 BamHI G'GATC_C Cuts at: 3646 4013 3646 Size: 367 5949 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2132 2286 2467 2753 3637 Size: 325 217 627 345 154 181 286 884 Cuts at: 3637 5092 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 884 627 345 325 286 217 181 154 BanII G_rGCy'C Cuts at: 1391 2085 2095 2117 2124 3215 3635 1391 Size: 694 10 22 7 1091 420 4072 Fragments arranged by size: 4072 1091 694 420 22 10 7 BbeI G_GCGC'C Cuts at: 2136 2136 Size: 6316 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3246 928 Size: 971 1347 3998 Fragments arranged by size: 3998 1347 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2754 2788 4507 4541 1903 Size: 34 817 34 1719 34 3678 Fragments arranged by size: 3678 1719 817 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4208 5418 4208 Size: 1210 5106 BclI T'GATC_A Cuts at: 1548 1548 Size: 6316 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3219 4927 Size: 107 122 71 631 605 337 1210 1708 Cuts at: 4927 136 Size: 1525 Fragments arranged by size: 1708 1525 1210 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6311 6311 Size: 6316 BlpI GC'TnA_GC Cuts at: 3733 3733 Size: 6316 Bme1580I G_kGCm'C Cuts at: 1790 2124 2756 3450 4377 5533 1790 Size: 334 632 694 927 1156 2573 Fragments arranged by size: 2573 1156 927 694 632 334 BmgBI CAC'GTC Cuts at: 1318 3136 1318 Size: 1818 4498 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2682 4047 5005 Size: 416 53 83 399 24 1265 1365 958 Cuts at: 5005 442 Size: 1753 Fragments arranged by size: 1753 1365 1265 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6316 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2099 2393 3107 3268 4976 1239 Size: 860 294 714 161 1708 2579 Fragments arranged by size: 2579 1708 860 714 294 161 Bpu10I CC'TnA_GC Cuts at: 2482 2482 Size: 6316 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3404 4441 5306 5604 3404 Size: 1037 865 298 4116 Fragments arranged by size: 4116 1037 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4979 882 Size: 617 3480 2219 Fragments arranged by size: 3480 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4107 493 Size: 711 2903 2702 Fragments arranged by size: 2903 2702 711 BsaBI GATnn'nnATC Cuts at: 2705 6310 2705 Size: 3605 2711 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2133 2384 4123 4505 Size: 53 83 186 936 600 251 1739 382 Cuts at: 4505 5140 275 Size: 635 1451 Fragments arranged by size: 1739 1451 936 635 600 382 251 186 83 53 BsaWI w'CCGG_w Cuts at: 2697 3273 4749 5147 5421 5568 5979 2697 Size: 576 1476 398 274 147 411 3034 Fragments arranged by size: 3034 1476 576 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2321 2351 2528 2558 1178 Size: 30 165 30 918 30 177 30 4936 Fragments arranged by size: 4936 918 177 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2342 2656 3104 3107 3155 1186 Size: 1156 314 448 3 48 4347 Fragments arranged by size: 4347 1156 448 314 48 3 BseYI C'CCAG_C Cuts at: 2866 5519 6015 2866 Size: 2653 496 3167 Fragments arranged by size: 3167 2653 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2600 2988 3463 3506 1163 Size: 573 864 388 475 43 3973 Fragments arranged by size: 3973 864 573 475 388 43 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3766 4527 5555 1540 Size: 275 186 1765 761 1028 2301 Fragments arranged by size: 2301 1765 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2281 4377 4462 5533 2281 Size: 2096 85 1071 3064 Fragments arranged by size: 3064 2096 1071 85 BsmI GAATG_Cn' Cuts at: 3827 3920 3827 Size: 93 6223 BsmBI CGTCTCn'nnnn_ Cuts at: 3240 3490 3240 Size: 250 6066 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2085 2095 2117 2124 2281 2756 3215 Size: 399 295 10 22 7 157 475 459 Cuts at: 3215 3450 3635 4377 4462 5533 1391 Size: 235 185 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 475 459 399 295 235 185 157 85 22 10 7 BspEI T'CCGG_A Cuts at: 2697 3273 5979 2697 Size: 576 2706 3034 Fragments arranged by size: 3034 2706 576 BspHI T'CATG_A Cuts at: 4205 5935 4205 Size: 1730 4586 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2296 3671 844 Size: 1452 1375 3489 Fragments arranged by size: 3489 1452 1375 BsrBI CCG'CTC Cuts at: 1394 1823 4203 1394 Size: 429 2380 3507 Fragments arranged by size: 3507 2380 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4811 4985 65 Size: 1183 3563 174 1396 Fragments arranged by size: 3563 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2129 4960 5147 1700 Size: 110 319 2831 187 2869 Fragments arranged by size: 2869 2831 319 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6316 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6316 BssSI C'ACGA_G Cuts at: 3576 4376 5388 3576 Size: 800 1012 4504 Fragments arranged by size: 4504 1012 800 BstAPI GCAn_nnn'nTGC Cuts at: 794 2041 2359 794 Size: 1247 318 4751 Fragments arranged by size: 4751 1247 318 BstBI TT'CG_AA Cuts at: 5120 5120 Size: 6316 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6316 BstYI r'GATC_y Cuts at: 1972 3020 3646 4013 4398 4415 5867 5953 5965 Size: 1048 626 367 385 17 1452 86 12 Cuts at: 5965 6311 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1048 626 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2748 2808 513 Size: 552 939 744 60 4021 Fragments arranged by size: 4021 939 744 552 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2161 487 Size: 683 56 935 4642 Fragments arranged by size: 4642 935 683 56 BtsI GCAGTG_nn' Cuts at: 2224 2971 3550 3832 4626 4646 2224 Size: 747 579 282 794 20 3894 Fragments arranged by size: 3894 794 747 579 282 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6316 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6281 DraI TTT'AAA Cuts at: 2952 3620 2952 Size: 668 5648 DraIII CAC_nnn'GTG Cuts at: 3016 3016 Size: 6316 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2576 3515 5323 809 Size: 507 1260 939 1808 1802 Fragments arranged by size: 1808 1802 1260 939 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2805 3171 3763 Size: 54 1425 50 275 18 975 366 592 Cuts at: 3763 4652 6281 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 975 889 592 366 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3763 1537 Size: 275 1951 4090 Fragments arranged by size: 4090 1951 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3582 4246 1353 Size: 2229 664 3423 Fragments arranged by size: 3423 2229 664 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2039 2125 2604 4903 5277 5423 Size: 1064 138 450 86 479 2299 374 146 Cuts at: 5423 387 Size: 1280 Fragments arranged by size: 2299 1280 1064 479 450 374 146 138 86 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2099 2393 3107 3268 4379 4976 5763 Size: 393 467 294 714 161 1111 597 787 Cuts at: 5763 6031 1239 Size: 268 1524 Fragments arranged by size: 1524 1111 787 714 597 467 393 294 268 161 EcoNI CCTnn'n_nnAGG Cuts at: 3422 3422 Size: 6316 EcoO109I rG'GnC_Cy Cuts at: 2120 3220 3517 2120 Size: 1100 297 4919 Fragments arranged by size: 4919 1100 297 EcoRI G'AATT_C Cuts at: 3625 4003 3625 Size: 378 5938 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3457 3457 Size: 6316 FspI TGC'GCA Cuts at: 4822 4822 Size: 6316 HaeII r_GCGC'y Cuts at: 1678 2065 2136 2649 2780 3442 5463 1678 Size: 387 71 513 131 662 2021 2531 Fragments arranged by size: 2531 2021 662 513 387 131 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2138 2170 2804 2836 3294 Size: 32 135 32 566 32 634 32 458 Cuts at: 3294 3326 4957 4989 5031 5063 1373 Size: 32 1631 32 42 32 2626 Fragments arranged by size: 2626 1631 634 566 458 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2656 2724 3660 3906 669 Size: 988 281 718 68 936 246 3079 Fragments arranged by size: 3079 988 936 718 281 246 68 HindIII A'AGCT_T Cuts at: 748 748 Size: 6316 HpaI GTT'AAC Cuts at: 3906 3906 Size: 6316 KasI G'GCGC_C Cuts at: 2132 2132 Size: 6316 KpnI G_GTAC'C Cuts at: 3641 3641 Size: 6316 MfeI C'AATT_G Cuts at: 3915 3915 Size: 6316 MluI A'CGCG_T Cuts at: 6192 6192 Size: 6316 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3513 5430 5614 1305 Size: 2208 1917 184 2007 Fragments arranged by size: 2208 2007 1917 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2807 3173 10 Size: 54 1425 343 975 366 3153 Fragments arranged by size: 3153 1425 975 366 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2813 2976 3188 4274 4633 4792 518 Size: 648 1647 163 212 1086 359 159 2042 Fragments arranged by size: 2042 1647 1086 648 359 212 163 159 MspA1I CmG'CkG Cuts at: 1606 2032 2292 2335 3085 3559 3732 4409 5557 Size: 426 260 43 750 474 173 677 1148 Cuts at: 5557 5802 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 750 677 474 426 260 245 173 43 NaeI GCC'GGC Cuts at: 2131 2131 Size: 6316 NarI GG'CG_CC Cuts at: 2133 2133 Size: 6316 NcoI C'CATG_G Cuts at: 513 1065 2004 2808 513 Size: 552 939 804 4021 Fragments arranged by size: 4021 939 804 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6316 NgoMIV G'CCGG_C Cuts at: 2129 2129 Size: 6316 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6316 NotI GC'GGCC_GC Cuts at: 3763 3763 Size: 6316 NspI r_CATG'y Cuts at: 1132 1229 2522 3193 3674 5219 1132 Size: 97 1293 671 481 1545 2229 Fragments arranged by size: 2229 1545 1293 671 481 97 PasI CC'CwG_GG Cuts at: 1348 3216 1348 Size: 1868 4448 PciI A'CATG_T Cuts at: 3189 5215 3189 Size: 2026 4290 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6316 PfoI T'CCnGG_A Cuts at: 3497 3497 Size: 6316 PmeI GTTT'AAAC Cuts at: 3620 3620 Size: 6316 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4385 4417 5898 5930 4385 Size: 32 1481 32 4771 Fragments arranged by size: 4771 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3517 3517 Size: 6316 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6316 PsiI TTA'TAA Cuts at: 3886 3886 Size: 6316 PspOMI G'GGCC_C Cuts at: 2120 2120 Size: 6316 PstI C_TGCA'G Cuts at: 830 2355 3668 830 Size: 1525 1313 3478 Fragments arranged by size: 3478 1525 1313 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6316 PvuII CAG'CTG Cuts at: 2335 3085 3559 2335 Size: 750 474 5092 Fragments arranged by size: 5092 750 474 SalI G'TCGA_C Cuts at: 1936 2654 3658 1936 Size: 718 1004 4594 Fragments arranged by size: 4594 1004 718 SbfI CC_TGCA'GG Cuts at: 3668 3668 Size: 6316 ScaI AGT'ACT Cuts at: 4564 6276 4564 Size: 1712 4604 SfcI C'TryA_G Cuts at: 826 1045 2351 3664 4799 5480 5671 826 Size: 219 1306 1313 1135 681 191 1471 Fragments arranged by size: 1471 1313 1306 1135 681 219 191 SfoI GGC'GCC Cuts at: 2134 2134 Size: 6316 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6316 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6316 SmaI CCC'GGG Cuts at: 1872 2118 2672 3643 1872 Size: 246 554 971 4545 Fragments arranged by size: 4545 971 554 246 SmlI C'TyrA_G Cuts at: 820 1017 1943 3419 4420 5321 5583 820 Size: 197 926 1476 1001 901 262 1553 Fragments arranged by size: 1553 1476 1001 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4107 493 Size: 3614 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6316 SphI G_CATG'C Cuts at: 1132 3674 1132 Size: 2542 3774 SspI AAT'ATT Cuts at: 5 52 4240 5 Size: 47 4188 2081 Fragments arranged by size: 4188 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6316 StyI C'CwwG_G Cuts at: 513 1065 2004 2532 2808 3026 3755 513 Size: 552 939 528 276 218 729 3074 Fragments arranged by size: 3074 939 729 552 528 276 218 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2038 4343 4360 4513 4698 5143 325 Size: 1255 458 2305 17 153 185 445 1498 Fragments arranged by size: 2305 1498 1255 458 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4562 6274 96 Size: 274 80 33 51 1443 2585 1712 138 Fragments arranged by size: 2585 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2321 2353 2720 2752 6276 6308 2321 Size: 32 367 32 3524 32 2329 Fragments arranged by size: 3524 2329 367 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2989 1316 Size: 1673 4643 XbaI T'CTAG_A Cuts at: 3652 3652 Size: 6316 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6316 XmaI C'CCGG_G Cuts at: 1870 2116 2670 3641 1870 Size: 246 554 971 4545 Fragments arranged by size: 4545 971 554 246 XmnI GAAnn'nnTTC Cuts at: 4445 4445 Size: 6316 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2385 4124 276 Size: 53 83 186 936 851 1739 2468 Fragments arranged by size: 2468 1739 936 851 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI