(Circular) (Six-base) MAPSORT of: pFN21AA1611.seq Check: 9227 from: 1 to: 7159 pFN21AA1611 7159 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4969 278 Size: 53 83 186 936 3433 2468 Fragments arranged by size: 3433 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2633 4502 1937 Size: 696 1869 4594 Fragments arranged by size: 4594 1869 696 Acc65I G'GTAC_C Cuts at: 4480 4480 Size: 7159 AclI AA'CG_TT Cuts at: 1563 5286 5659 1563 Size: 3723 373 3063 Fragments arranged by size: 3723 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2953 3373 3541 4129 4381 5222 6606 6874 Size: 1321 420 168 588 252 841 1384 268 Cuts at: 6874 1632 Size: 1917 Fragments arranged by size: 1917 1384 1321 841 588 420 268 252 168 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6962 AflIII A'CryG_T Cuts at: 1203 6058 7035 1203 Size: 4855 977 1327 Fragments arranged by size: 4855 1327 977 AgeI A'CCGG_T Cuts at: 5990 5990 Size: 7159 AhdI GACnn_n'nnGTC Cuts at: 5888 5888 Size: 7159 AleI CACnn'nnGTG Cuts at: 1166 2351 1166 Size: 1185 5974 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7159 AlwNI CAG_nnn'CTG Cuts at: 4902 6474 4902 Size: 1572 5587 ApaLI G'TGCA_C Cuts at: 5216 6372 5216 Size: 1156 6003 ApoI r'AATT_y Cuts at: 1493 2051 2670 2809 2835 3003 3145 3171 3481 Size: 558 619 139 26 168 142 26 310 Cuts at: 3481 3507 3675 3927 4263 4468 4694 4846 6955 Size: 26 168 252 336 205 226 152 2109 Cuts at: 6955 1493 Size: 1697 Fragments arranged by size: 2109 1697 619 558 336 310 252 226 205 168 168 152 142 139 26 26 26 AseI AT'TA_AT Cuts at: 160 1032 5713 160 Size: 872 4681 1606 Fragments arranged by size: 4681 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7159 AvaI C'yCGr_G Cuts at: 1870 1943 4484 1870 Size: 73 2541 4545 Fragments arranged by size: 4545 2541 73 BamHI G'GATC_C Cuts at: 4489 4856 4489 Size: 367 6792 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4480 5935 618 Size: 325 217 627 2693 1455 1842 Fragments arranged by size: 2693 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2202 4478 1391 Size: 811 2276 4072 Fragments arranged by size: 4072 2276 811 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2391 2959 3715 4051 4387 928 Size: 971 492 568 756 336 336 3700 Fragments arranged by size: 3700 971 756 568 492 336 336 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5350 5384 1903 Size: 34 3413 34 3678 Fragments arranged by size: 3678 3413 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5051 6261 5051 Size: 1210 5949 BclI T'GATC_A Cuts at: 1548 1548 Size: 7159 BfrBI ATG'CAT Cuts at: 3397 3985 4435 3397 Size: 588 450 6121 Fragments arranged by size: 6121 588 450 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5770 136 Size: 107 122 71 631 605 4098 1525 Fragments arranged by size: 4098 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7154 7154 Size: 7159 BlpI GC'TnA_GC Cuts at: 4576 4576 Size: 7159 Bme1580I G_kGCm'C Cuts at: 1790 5220 6376 1790 Size: 3430 1156 2573 Fragments arranged by size: 3430 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2110 1318 Size: 792 6367 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4197 4281 4890 Size: 416 53 83 399 24 2780 84 609 Cuts at: 4890 5848 442 Size: 958 1753 Fragments arranged by size: 2780 1753 958 609 416 399 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7159 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3660 3660 Size: 7159 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2949 3033 3117 3201 3285 3537 3621 3705 Size: 1710 84 84 84 84 252 84 84 Cuts at: 3705 3789 3873 3957 4041 4377 5819 1239 Size: 84 84 84 84 336 1442 2579 Fragments arranged by size: 2579 1710 1442 336 252 84 84 84 84 84 84 84 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5284 6149 6447 5284 Size: 865 298 5996 Fragments arranged by size: 5996 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5822 882 Size: 617 4323 2219 Fragments arranged by size: 4323 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4950 493 Size: 711 3746 2702 Fragments arranged by size: 3746 2702 711 BsaBI GATnn'nnATC Cuts at: 7153 7153 Size: 7159 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4966 5348 5983 275 Size: 53 83 186 936 3433 382 635 1451 Fragments arranged by size: 3433 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4103 4439 5592 5990 6264 6411 6822 4103 Size: 336 1153 398 274 147 411 4440 Fragments arranged by size: 4440 1153 411 398 336 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6934 Fragments arranged by size: 6934 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2078 1186 Size: 892 6267 BseYI C'CCAG_C Cuts at: 6362 6858 6362 Size: 496 6663 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6586 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4609 5370 6398 1540 Size: 275 186 2608 761 1028 2301 Fragments arranged by size: 2608 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5220 5305 6376 5220 Size: 85 1071 6003 Fragments arranged by size: 6003 1071 85 BsmI GAATG_Cn' Cuts at: 3397 3464 3884 3985 4437 4670 4763 3397 Size: 67 420 101 452 233 93 5793 Fragments arranged by size: 5793 452 420 233 101 93 67 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2202 4478 5220 5305 6376 1391 Size: 399 412 2276 742 85 1071 2174 Fragments arranged by size: 2276 2174 1071 742 412 399 85 BspEI T'CCGG_A Cuts at: 6822 6822 Size: 7159 BspHI T'CATG_A Cuts at: 2710 2950 3874 5048 6778 2710 Size: 240 924 1174 1730 3091 Fragments arranged by size: 3091 1730 1174 924 240 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4514 844 Size: 3670 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5046 1394 Size: 429 3223 3507 Fragments arranged by size: 3507 3223 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3208 3460 4132 5654 5828 65 Size: 1183 1960 252 672 1522 174 1396 Fragments arranged by size: 1960 1522 1396 1183 672 252 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5803 5990 1700 Size: 110 3993 187 2869 Fragments arranged by size: 3993 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2291 4292 96 Size: 2195 2001 2963 Fragments arranged by size: 2963 2195 2001 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7159 BssSI C'ACGA_G Cuts at: 5219 6231 5219 Size: 1012 6147 BstAPI GCAn_nnn'nTGC Cuts at: 794 2871 3207 3459 794 Size: 2077 336 252 4494 Fragments arranged by size: 4494 2077 336 252 BstBI TT'CG_AA Cuts at: 2813 5963 2813 Size: 3150 4009 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7159 BstYI r'GATC_y Cuts at: 1972 2094 4489 4856 5241 5258 6710 6796 6808 Size: 122 2395 367 385 17 1452 86 12 Cuts at: 6808 7154 1972 Size: 346 1977 Fragments arranged by size: 2395 1977 1452 385 367 346 122 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5668 Fragments arranged by size: 5668 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6420 Fragments arranged by size: 6420 683 56 BtsI GCAGTG_nn' Cuts at: 2872 4675 5469 5489 2872 Size: 1803 794 20 4542 Fragments arranged by size: 4542 1803 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7159 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4114 4149 529 Size: 35 3550 35 3539 Fragments arranged by size: 3550 3539 35 35 DraI TTT'AAA Cuts at: 4463 4463 Size: 7159 DraIII CAC_nnn'GTG Cuts at: 2350 2350 Size: 7159 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6166 809 Size: 507 4850 1802 Fragments arranged by size: 4850 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 4606 5495 Size: 54 1425 50 275 18 213 2563 889 Cuts at: 5495 7124 8 Size: 1629 43 Fragments arranged by size: 2563 1629 1425 889 275 213 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4606 1537 Size: 275 2794 4090 Fragments arranged by size: 4090 2794 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2207 2315 2506 3140 5089 1353 Size: 854 108 191 634 1949 3423 Fragments arranged by size: 3423 1949 854 634 191 108 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5746 6120 6266 387 Size: 1064 138 4157 374 146 1280 Fragments arranged by size: 4157 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2949 2953 3033 3117 3201 3285 3373 Size: 393 1317 4 80 84 84 84 88 Cuts at: 3373 3537 3541 3621 3705 3789 3873 3957 4041 Size: 164 4 80 84 84 84 84 84 Cuts at: 4041 4129 4377 4381 5222 5819 6606 6874 1239 Size: 88 248 4 841 597 787 268 1524 Fragments arranged by size: 1524 1317 841 787 597 393 268 248 164 88 88 84 84 84 84 84 84 84 84 80 80 4 4 4 EcoNI CCTnn'n_nnAGG Cuts at: 2015 2015 Size: 7159 EcoRI G'AATT_C Cuts at: 2051 2809 2835 3003 3171 3507 3675 3927 4263 Size: 758 26 168 168 336 168 252 336 Cuts at: 4263 4468 4846 2051 Size: 205 378 4364 Fragments arranged by size: 4364 758 378 336 336 252 205 168 168 168 26 EcoRV GAT'ATC Cuts at: 2278 2278 Size: 7159 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3846 3846 Size: 7159 FspI TGC'GCA Cuts at: 5665 5665 Size: 7159 HaeII r_GCGC'y Cuts at: 1678 6306 1678 Size: 4628 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3568 3600 4396 4428 5800 Size: 32 135 32 1996 32 796 32 1372 Cuts at: 5800 5832 5874 5906 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1996 1372 796 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2028 4503 4749 669 Size: 988 281 90 2475 246 3079 Fragments arranged by size: 3079 2475 988 281 246 90 HindIII A'AGCT_T Cuts at: 748 2528 2554 748 Size: 1780 26 5353 Fragments arranged by size: 5353 1780 26 HpaI GTT'AAC Cuts at: 4749 4749 Size: 7159 KpnI G_GTAC'C Cuts at: 4484 4484 Size: 7159 MfeI C'AATT_G Cuts at: 4758 4758 Size: 7159 MluI A'CGCG_T Cuts at: 7035 7035 Size: 7159 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2097 2163 2337 6273 6457 1305 Size: 792 66 174 3936 184 2007 Fragments arranged by size: 3936 2007 792 184 174 66 MscI TGG'CCA Cuts at: 10 64 1489 1832 2045 10 Size: 54 1425 343 213 5124 Fragments arranged by size: 5124 1425 343 213 54 MslI CAynn'nnrTG Cuts at: 518 1166 2351 2955 3348 5117 5476 5635 518 Size: 648 1185 604 393 1769 359 159 2042 Fragments arranged by size: 2042 1769 1185 648 604 393 359 159 MspA1I CmG'CkG Cuts at: 1606 4575 5252 6400 6645 1606 Size: 2969 677 1148 245 2120 Fragments arranged by size: 2969 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5668 Fragments arranged by size: 5668 939 552 NdeI CA'TA_TG Cuts at: 387 4341 387 Size: 3954 3205 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7159 NotI GC'GGCC_GC Cuts at: 4606 4606 Size: 7159 NsiI A_TGCA'T Cuts at: 3399 3987 4437 3399 Size: 588 450 6121 Fragments arranged by size: 6121 588 450 NspI r_CATG'y Cuts at: 1132 1229 4517 6062 1132 Size: 97 3288 1545 2229 Fragments arranged by size: 3288 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7159 PciI A'CATG_T Cuts at: 6058 6058 Size: 7159 PflMI CCAn_nnn'nTGG Cuts at: 1108 2350 1108 Size: 1242 5917 PmeI GTTT'AAAC Cuts at: 4463 4463 Size: 7159 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5228 5260 6741 6773 5228 Size: 32 1481 32 5614 Fragments arranged by size: 5614 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7159 PsiI TTA'TAA Cuts at: 2775 3158 4729 2775 Size: 383 1571 5205 Fragments arranged by size: 5205 1571 383 PstI C_TGCA'G Cuts at: 830 2583 4511 830 Size: 1753 1928 3478 Fragments arranged by size: 3478 1928 1753 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7159 SalI G'TCGA_C Cuts at: 1936 4501 1936 Size: 2565 4594 SapI GCTCTTCn'nnn_ Cuts at: 2207 2315 3140 2207 Size: 108 825 6226 Fragments arranged by size: 6226 825 108 SbfI CC_TGCA'GG Cuts at: 4511 4511 Size: 7159 ScaI AGT'ACT Cuts at: 5407 7119 5407 Size: 1712 5447 SfcI C'TryA_G Cuts at: 826 1045 2579 4507 5642 6323 6514 826 Size: 219 1534 1928 1135 681 191 1471 Fragments arranged by size: 1928 1534 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7159 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7159 SmaI CCC'GGG Cuts at: 1872 4486 1872 Size: 2614 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5263 6164 6426 820 Size: 197 926 3320 901 262 1553 Fragments arranged by size: 3320 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4950 493 Size: 4457 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7159 SphI G_CATG'C Cuts at: 1132 4517 1132 Size: 3385 3774 SspI AAT'ATT Cuts at: 5 52 2170 5083 5 Size: 47 2118 2913 2081 Fragments arranged by size: 2913 2118 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7159 StyI C'CwwG_G Cuts at: 513 1065 2004 4598 513 Size: 552 939 2594 3074 Fragments arranged by size: 3074 2594 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4184 5186 5203 5356 5541 5986 325 Size: 1255 2604 1002 17 153 185 445 1498 Fragments arranged by size: 2604 1498 1255 1002 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2291 2327 4292 Size: 274 80 33 51 1443 314 36 1965 Cuts at: 4292 5405 7117 96 Size: 1113 1712 138 Fragments arranged by size: 1965 1712 1443 1113 314 274 138 80 51 36 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7119 7151 7119 Size: 32 7127 Tth111I GACn'n_nGTC Cuts at: 1316 2151 1316 Size: 835 6324 XbaI T'CTAG_A Cuts at: 4495 4495 Size: 7159 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7159 XmaI C'CCGG_G Cuts at: 1870 4484 1870 Size: 2614 4545 XmnI GAAnn'nnTTC Cuts at: 3554 3679 4142 5288 3554 Size: 125 463 1146 5425 Fragments arranged by size: 5425 1146 463 125 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4967 276 Size: 53 83 186 936 3433 2468 Fragments arranged by size: 3433 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI