(Circular) (Six-base) MAPSORT of: pFN21AA1579.seq Check: 7736 from: 1 to: 6739 pFN21AA1579 6739 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4549 278 Size: 53 83 186 936 3013 2468 Fragments arranged by size: 3013 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2620 4035 4082 1937 Size: 683 1415 47 4594 Fragments arranged by size: 4594 1415 683 47 Acc65I G'GTAC_C Cuts at: 4060 4060 Size: 6739 AclI AA'CG_TT Cuts at: 1563 4866 5239 1563 Size: 3303 373 3063 Fragments arranged by size: 3303 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2399 2661 2880 3284 3536 4802 6186 6454 Size: 767 262 219 404 252 1266 1384 268 Cuts at: 6454 1632 Size: 1917 Fragments arranged by size: 1917 1384 1266 767 404 268 262 252 219 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6542 AflIII A'CryG_T Cuts at: 1203 2977 5638 6615 1203 Size: 1774 2661 977 1327 Fragments arranged by size: 2661 1774 1327 977 AgeI A'CCGG_T Cuts at: 5570 5570 Size: 6739 AhdI GACnn_n'nnGTC Cuts at: 5468 5468 Size: 6739 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6739 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6739 AlwNI CAG_nnn'CTG Cuts at: 2247 3647 4482 6054 2247 Size: 1400 835 1572 2932 Fragments arranged by size: 2932 1572 1400 835 ApaI G_GGCC'C Cuts at: 2102 2102 Size: 6739 ApaLI G'TGCA_C Cuts at: 4796 5952 4796 Size: 1156 5583 ApoI r'AATT_y Cuts at: 1493 2754 3526 3693 3721 4048 4274 4426 6535 Size: 1261 772 167 28 327 226 152 2109 Cuts at: 6535 1493 Size: 1697 Fragments arranged by size: 2109 1697 1261 772 327 226 167 152 28 AseI AT'TA_AT Cuts at: 160 1032 3230 5293 160 Size: 872 2198 2063 1606 Fragments arranged by size: 2198 2063 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6739 AvaI C'yCGr_G Cuts at: 1870 1943 4064 1870 Size: 73 2121 4545 Fragments arranged by size: 4545 2121 73 BamHI G'GATC_C Cuts at: 4069 4436 4069 Size: 367 6372 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2123 4060 5515 618 Size: 325 217 627 336 1937 1455 1842 Fragments arranged by size: 1937 1842 1455 627 336 325 217 BanII G_rGCy'C Cuts at: 1391 2102 2685 3285 4058 1391 Size: 711 583 600 773 4072 Fragments arranged by size: 4072 773 711 600 583 BbeI G_GCGC'C Cuts at: 2127 2127 Size: 6739 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5768 BbvCI CC'TCA_GC Cuts at: 2104 2104 Size: 6739 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4930 4964 1903 Size: 34 2993 34 3678 Fragments arranged by size: 3678 2993 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4631 5841 4631 Size: 1210 5529 BclI T'GATC_A Cuts at: 1548 1548 Size: 6739 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5350 136 Size: 107 122 71 631 605 337 3341 1525 Fragments arranged by size: 3341 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6734 6734 Size: 6739 BlpI GC'TnA_GC Cuts at: 2198 4156 2198 Size: 1958 4781 Bme1580I G_kGCm'C Cuts at: 1790 2102 2454 3477 3992 4800 5956 1790 Size: 312 352 1023 515 808 1156 2573 Fragments arranged by size: 2573 1156 1023 808 515 352 312 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6739 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2715 3076 3735 Size: 416 53 83 399 24 1298 361 659 Cuts at: 3735 4470 5428 442 Size: 735 958 1753 Fragments arranged by size: 1753 1298 958 735 659 416 399 361 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6739 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2627 2952 5399 1239 Size: 1388 325 2447 2579 Fragments arranged by size: 2579 2447 1388 325 Bpu10I CC'TnA_GC Cuts at: 2104 2104 Size: 6739 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4864 5729 6027 4864 Size: 865 298 5576 Fragments arranged by size: 5576 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5402 882 Size: 617 3903 2219 Fragments arranged by size: 3903 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4530 493 Size: 711 3326 2702 Fragments arranged by size: 3326 2702 711 BsaBI GATnn'nnATC Cuts at: 2664 6733 2664 Size: 4069 2670 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2124 4546 4928 5563 Size: 53 83 186 936 591 2422 382 635 Cuts at: 5563 275 Size: 1451 Fragments arranged by size: 2422 1451 936 635 591 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5172 5570 5844 5991 6402 5172 Size: 398 274 147 411 5509 Fragments arranged by size: 5509 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6514 Fragments arranged by size: 6514 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2174 1186 Size: 988 5751 BseYI C'CCAG_C Cuts at: 2068 3286 3464 5942 6438 2068 Size: 1218 178 2478 496 2369 Fragments arranged by size: 2478 2369 1218 496 178 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2135 3291 1163 Size: 573 399 1156 4611 Fragments arranged by size: 4611 1156 573 399 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4189 4950 5978 1540 Size: 275 186 2188 761 1028 2301 Fragments arranged by size: 2301 2188 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2685 2929 4800 4885 5956 2685 Size: 244 1871 85 1071 3468 Fragments arranged by size: 3468 1871 1071 244 85 BsmI GAATG_Cn' Cuts at: 3804 3979 4250 4343 3804 Size: 175 271 93 6200 Fragments arranged by size: 6200 271 175 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2016 2016 Size: 6739 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2102 2454 2685 2929 3285 3477 3992 Size: 399 312 352 231 244 356 192 515 Cuts at: 3992 4058 4800 4885 5956 1391 Size: 66 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 515 399 356 352 312 244 231 192 85 66 BspEI T'CCGG_A Cuts at: 6402 6402 Size: 6739 BspHI T'CATG_A Cuts at: 2260 4628 6358 2260 Size: 2368 1730 2641 Fragments arranged by size: 2641 2368 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2238 4094 844 Size: 1394 1856 3489 Fragments arranged by size: 3489 1856 1394 BsrBI CCG'CTC Cuts at: 1394 1823 4626 1394 Size: 429 2803 3507 Fragments arranged by size: 3507 2803 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3450 3858 5234 5408 65 Size: 1183 2202 408 1376 174 1396 Fragments arranged by size: 2202 1396 1376 1183 408 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5383 5570 1700 Size: 110 3573 187 2869 Fragments arranged by size: 3573 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6739 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6739 BssSI C'ACGA_G Cuts at: 2117 4799 5811 2117 Size: 2682 1012 3045 Fragments arranged by size: 3045 2682 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2185 2645 794 Size: 1391 460 4888 Fragments arranged by size: 4888 1391 460 BstBI TT'CG_AA Cuts at: 5543 5543 Size: 6739 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6739 BstXI CCAn_nnnn'nTGG Cuts at: 3089 3089 Size: 6739 BstYI r'GATC_y Cuts at: 1972 4069 4436 4821 4838 6290 6376 6388 6734 Size: 2097 367 385 17 1452 86 12 346 Cuts at: 6734 1972 Size: 1977 Fragments arranged by size: 2097 1977 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 4036 4036 Size: 6739 Bsu36I CC'TnA_GG Cuts at: 2157 2157 Size: 6739 BtgI C'CryG_G Cuts at: 513 1065 2004 3504 513 Size: 552 939 1500 3748 Fragments arranged by size: 3748 1500 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6000 Fragments arranged by size: 6000 683 56 BtsI GCAGTG_nn' Cuts at: 2835 2844 4255 5049 5069 2835 Size: 9 1411 794 20 4505 Fragments arranged by size: 4505 1411 794 20 9 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6739 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6704 DraI TTT'AAA Cuts at: 4043 4043 Size: 6739 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2405 5746 809 Size: 507 1089 3341 1802 Fragments arranged by size: 3341 1802 1089 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2542 4186 5075 Size: 54 1425 50 275 18 712 1644 889 Cuts at: 5075 6704 8 Size: 1629 43 Fragments arranged by size: 1644 1629 1425 889 712 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4186 1537 Size: 275 2374 4090 Fragments arranged by size: 4090 2374 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2466 2736 2856 3783 4669 1353 Size: 1113 270 120 927 886 3423 Fragments arranged by size: 3423 1113 927 886 270 120 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5326 5700 5846 387 Size: 1064 138 3737 374 146 1280 Fragments arranged by size: 3737 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2683 2683 Size: 6739 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2399 2627 2661 2880 2952 3284 3536 Size: 393 767 228 34 219 72 332 252 Cuts at: 3536 4802 5399 6186 6454 1239 Size: 1266 597 787 268 1524 Fragments arranged by size: 1524 1266 787 767 597 393 332 268 252 228 219 72 34 EcoNI CCTnn'n_nnAGG Cuts at: 2234 2234 Size: 6739 EcoO109I rG'GnC_Cy Cuts at: 2098 2099 2098 Size: 1 6738 EcoRI G'AATT_C Cuts at: 4048 4426 4048 Size: 378 6361 FspI TGC'GCA Cuts at: 5245 5245 Size: 6739 HaeII r_GCGC'y Cuts at: 1678 2127 2939 5886 1678 Size: 449 812 2947 2531 Fragments arranged by size: 2947 2531 812 449 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2359 2391 2956 2988 3379 Size: 32 135 32 787 32 565 32 391 Cuts at: 3379 3411 3803 3835 5380 5412 5454 5486 1373 Size: 32 392 32 1545 32 42 32 2626 Fragments arranged by size: 2626 1545 787 565 392 391 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3973 4083 4329 669 Size: 988 281 2035 110 246 3079 Fragments arranged by size: 3079 2035 988 281 246 110 HindIII A'AGCT_T Cuts at: 748 3580 748 Size: 2832 3907 HpaI GTT'AAC Cuts at: 4329 4329 Size: 6739 KasI G'GCGC_C Cuts at: 2123 2123 Size: 6739 KpnI G_GTAC'C Cuts at: 4064 4064 Size: 6739 MfeI C'AATT_G Cuts at: 4338 4338 Size: 6739 MluI A'CGCG_T Cuts at: 6615 6615 Size: 6739 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3416 5853 6037 1305 Size: 2111 2437 184 2007 Fragments arranged by size: 2437 2111 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2544 10 Size: 54 1425 343 712 4205 Fragments arranged by size: 4205 1425 712 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3357 4697 5056 5215 518 Size: 648 2191 1340 359 159 2042 Fragments arranged by size: 2191 2042 1340 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2182 2191 3290 3413 4155 4832 5980 6225 Size: 576 9 1099 123 742 677 1148 245 Cuts at: 6225 1606 Size: 2120 Fragments arranged by size: 2120 1148 1099 742 677 576 245 123 9 NarI GG'CG_CC Cuts at: 2124 2124 Size: 6739 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5248 Fragments arranged by size: 5248 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6739 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6739 NotI GC'GGCC_GC Cuts at: 4186 4186 Size: 6739 NspI r_CATG'y Cuts at: 1132 1229 3427 4097 5642 1132 Size: 97 2198 670 1545 2229 Fragments arranged by size: 2229 2198 1545 670 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6739 PciI A'CATG_T Cuts at: 5638 5638 Size: 6739 PflMI CCAn_nnn'nTGG Cuts at: 1108 3878 3953 1108 Size: 2770 75 3894 Fragments arranged by size: 3894 2770 75 PfoI T'CCnGG_A Cuts at: 2929 2929 Size: 6739 PmeI GTTT'AAAC Cuts at: 4043 4043 Size: 6739 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4808 4840 6321 6353 4808 Size: 32 1481 32 5194 Fragments arranged by size: 5194 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6739 PsiI TTA'TAA Cuts at: 4309 4309 Size: 6739 PspOMI G'GGCC_C Cuts at: 2098 2098 Size: 6739 PstI C_TGCA'G Cuts at: 830 2181 4091 830 Size: 1351 1910 3478 Fragments arranged by size: 3478 1910 1351 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6739 PvuII CAG'CTG Cuts at: 3290 3413 3290 Size: 123 6616 SacI G_AGCT'C Cuts at: 2685 2685 Size: 6739 SalI G'TCGA_C Cuts at: 1936 4081 1936 Size: 2145 4594 SapI GCTCTTCn'nnn_ Cuts at: 2736 2736 Size: 6739 SbfI CC_TGCA'GG Cuts at: 4091 4091 Size: 6739 ScaI AGT'ACT Cuts at: 4987 6699 4987 Size: 1712 5027 SfcI C'TryA_G Cuts at: 826 1045 2177 2527 2728 3074 4087 5222 5903 Size: 219 1132 350 201 346 1013 1135 681 Cuts at: 5903 6094 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1132 1013 681 350 346 219 201 191 SfoI GGC'GCC Cuts at: 2125 2125 Size: 6739 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6739 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6739 SmaI CCC'GGG Cuts at: 1872 4066 1872 Size: 2194 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4843 5744 6006 820 Size: 197 926 2900 901 262 1553 Fragments arranged by size: 2900 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4530 493 Size: 4037 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6739 SphI G_CATG'C Cuts at: 1132 4097 1132 Size: 2965 3774 SspI AAT'ATT Cuts at: 5 52 2291 2381 2506 3247 4663 5 Size: 47 2239 90 125 741 1416 2081 Fragments arranged by size: 2239 2081 1416 741 125 90 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6739 StyI C'CwwG_G Cuts at: 513 1065 2004 4178 513 Size: 552 939 2174 3074 Fragments arranged by size: 3074 2174 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4766 4783 4936 5121 5566 325 Size: 1255 3186 17 153 185 445 1498 Fragments arranged by size: 3186 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2948 3025 4985 Size: 274 80 33 51 1443 971 77 1960 Cuts at: 4985 6697 96 Size: 1712 138 Fragments arranged by size: 1960 1712 1443 971 274 138 80 77 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6699 6731 6699 Size: 32 6707 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6739 XbaI T'CTAG_A Cuts at: 4075 4075 Size: 6739 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3511 3511 Size: 6739 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6739 XmaI C'CCGG_G Cuts at: 1870 4064 1870 Size: 2194 4545 XmnI GAAnn'nnTTC Cuts at: 2487 4868 2487 Size: 2381 4358 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4547 276 Size: 53 83 186 936 3013 2468 Fragments arranged by size: 3013 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI