Restriction map for
pFN21AA1539
(Circular) (Six-base) MAPSORT of: pFN21AA1539.seq Check: 9499 from: 1 to: 6319
pFN21AA1539 6319 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2877 2877
Size: 6319
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4129 278
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2145 3662 1937
Size: 208 1517 4594
Fragments arranged by size:
4594 1517 208
Acc65I G'GTAC_C
Cuts at: 2177 3640 2177
Size: 1463 4856
AclI AA'CG_TT
Cuts at: 1563 4446 4819 1563
Size: 2883 373 3063
Fragments arranged by size:
3063 2883 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2261 2304 2400 2972 4382 5766 6034 1632
Size: 629 43 96 572 1410 1384 268 1917
Fragments arranged by size:
1917 1410 1384 629 572 268 96 43
AflII C'TTAA_G
Cuts at: 820 1017 3493 820
Size: 197 2476 3646
Fragments arranged by size:
3646 2476 197
AflIII A'CryG_T
Cuts at: 1203 5218 6195 1203
Size: 4015 977 1327
Fragments arranged by size:
4015 1327 977
AgeI A'CCGG_T
Cuts at: 5150 5150
Size: 6319
AhdI GACnn_n'nnGTC
Cuts at: 5048 5048
Size: 6319
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6319
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2483 2598 1184
Size: 1299 115 4905
Fragments arranged by size:
4905 1299 115
AlwNI CAG_nnn'CTG
Cuts at: 2481 2612 4062 5634 2481
Size: 131 1450 1572 3166
Fragments arranged by size:
3166 1572 1450 131
ApaI G_GGCC'C
Cuts at: 2086 2200 2883 2086
Size: 114 683 5522
Fragments arranged by size:
5522 683 114
ApaLI G'TGCA_C
Cuts at: 4376 5532 4376
Size: 1156 5163
ApoI r'AATT_y
Cuts at: 1493 3628 3854 4006 6115 1493
Size: 2135 226 152 2109 1697
Fragments arranged by size:
2135 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4873 160
Size: 872 3841 1606
Fragments arranged by size:
3841 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
AvaI C'yCGr_G
Cuts at: 1870 1943 2138 2232 2678 3644 1870
Size: 73 195 94 446 966 4545
Fragments arranged by size:
4545 966 446 195 94 73
AvrII C'CTAG_G
Cuts at: 2642 2642
Size: 6319
BamHI G'GATC_C
Cuts at: 2804 3649 4016 2804
Size: 845 367 5107
Fragments arranged by size:
5107 845 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2177 2796 3278 3400 3640
Size: 325 217 627 390 619 482 122 240
Cuts at: 3640 5095 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 619 482 390 325 240
217 122
BanII G_rGCy'C
Cuts at: 1391 2086 2200 2307 2606 2883 2944 2963 3002
Size: 695 114 107 299 277 61 19 39
Cuts at: 3002 3479 3558 3638 1391
Size: 477 79 80 4072
Fragments arranged by size:
4072 695 477 299 277 114 107 80
79 61 39 19
BbeI G_GCGC'C
Cuts at: 2800 2800
Size: 6319
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5348
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4510 4544 1903
Size: 34 50 34 2489 34 3678
Fragments arranged by size:
3678 2489 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2164 4211 5421 2164
Size: 2047 1210 3062
Fragments arranged by size:
3062 2047 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6319
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4930 136
Size: 107 122 71 631 605 3258 1525
Fragments arranged by size:
3258 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6314 6314
Size: 6319
BlpI GC'TnA_GC
Cuts at: 2957 2996 3736 2957
Size: 39 740 5540
Fragments arranged by size:
5540 740 39
Bme1580I G_kGCm'C
Cuts at: 1790 2086 2200 2673 2883 3270 3281 4380 5536
Size: 296 114 473 210 387 11 1099 1156
Cuts at: 5536 1790
Size: 2573
Fragments arranged by size:
2573 1156 1099 473 387 296 210 114
11
BmgBI CAC'GTC
Cuts at: 1318 3157 1318
Size: 1839 4480
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2491 2600 3000
Size: 416 53 83 399 24 1074 109 400
Cuts at: 3000 4050 5008 442
Size: 1050 958 1753
Fragments arranged by size:
1753 1074 1050 958 416 400 399 109
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2052 2072 2446 2526 2766 2881 3560 4979
Size: 813 20 374 80 240 115 679 1419
Cuts at: 4979 1239
Size: 2579
Fragments arranged by size:
2579 1419 813 679 374 240 115 80
20
Bpu10I CC'TnA_GC
Cuts at: 3484 3484
Size: 6319
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4444 5309 5607 4444
Size: 865 298 5156
Fragments arranged by size:
5156 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4982 882
Size: 617 3483 2219
Fragments arranged by size:
3483 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2014 4110 493
Size: 711 810 2096 2702
Fragments arranged by size:
2702 2096 810 711
BsaBI GATnn'nnATC
Cuts at: 6313 6313
Size: 6319
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2797 4126 4508 5143
Size: 53 83 186 936 1264 1329 382 635
Cuts at: 5143 275
Size: 1451
Fragments arranged by size:
1451 1329 1264 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2976 3471 4752 5150 5424 5571 5982 2976
Size: 495 1281 398 274 147 411 3313
Fragments arranged by size:
3313 1281 495 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2471 2501 3241 3271 1178
Size: 30 165 30 1068 30 740 30 4226
Fragments arranged by size:
4226 1068 740 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3428 3434 1186
Size: 2242 6 4071
Fragments arranged by size:
4071 2242 6
BseYI C'CCAG_C
Cuts at: 2116 2257 2329 2612 3049 3150 5522 6018 2116
Size: 141 72 283 437 101 2372 496 2417
Fragments arranged by size:
2417 2372 496 437 283 141 101 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2036 2537 3443 1163
Size: 573 300 501 906 4039
Fragments arranged by size:
4039 906 573 501 300
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3769 4530 5558 1540
Size: 275 186 1768 761 1028 2301
Fragments arranged by size:
2301 1768 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2685 3479 4380 4465 5536 2685
Size: 794 901 85 1071 3468
Fragments arranged by size:
3468 1071 901 794 85
BsmI GAATG_Cn'
Cuts at: 3830 3923 3830
Size: 93 6226
BsmBI CGTCTCn'nnnn_
Cuts at: 2034 2034
Size: 6319
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2086 2200 2307 2606 2673 2685 2883
Size: 399 296 114 107 299 67 12 198
Cuts at: 2883 2944 2963 3002 3270 3281 3479 3558 3638
Size: 61 19 39 268 11 198 79 80
Cuts at: 3638 4380 4465 5536 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 299 296 268 198
198 114 107 85 80 79 67 61
39 19 12 11
BspEI T'CCGG_A
Cuts at: 2976 3471 5982 2976
Size: 495 2511 3313
Fragments arranged by size:
3313 2511 495
BspHI T'CATG_A
Cuts at: 4208 5938 4208
Size: 1730 4589
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2877 3674 844
Size: 2033 797 3489
Fragments arranged by size:
3489 2033 797
BsrBI CCG'CTC
Cuts at: 1394 1823 3483 4206 1394
Size: 429 1660 723 3507
Fragments arranged by size:
3507 1660 723 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2629 3068 4814 4988 65
Size: 1183 1381 439 1746 174 1396
Fragments arranged by size:
1746 1396 1381 1183 439 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4963 5150 1700
Size: 110 3153 187 2869
Fragments arranged by size:
3153 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6319
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6319
BssSI C'ACGA_G
Cuts at: 4379 5391 4379
Size: 1012 5307
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6319
BstBI TT'CG_AA
Cuts at: 2968 5123 2968
Size: 2155 4164
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6319
BstYI r'GATC_y
Cuts at: 1972 2494 2804 3232 3649 4016 4401 4418 5870
Size: 522 310 428 417 367 385 17 1452
Cuts at: 5870 5956 5968 6314 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 522 428 417 385 367 346
310 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3464 3464
Size: 6319
BtgI C'CryG_G
Cuts at: 513 1065 2365 2919 513
Size: 552 1300 554 3913
Fragments arranged by size:
3913 1300 554 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3371 487
Size: 683 56 2145 3435
Fragments arranged by size:
3435 2145 683 56
BtsI GCAGTG_nn'
Cuts at: 2658 3835 4629 4649 2658
Size: 1177 794 20 4328
Fragments arranged by size:
4328 1177 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6319
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6284
DraI TTT'AAA
Cuts at: 3623 3623
Size: 6319
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5326 809
Size: 507 4010 1802
Fragments arranged by size:
4010 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2343 3097 3766
Size: 54 1425 50 275 18 513 754 669
Cuts at: 3766 4655 6284 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 754 669 513 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3766 1537
Size: 275 1954 4090
Fragments arranged by size:
4090 1954 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2237 2769 4249 1353
Size: 884 532 1480 3423
Fragments arranged by size:
3423 1480 884 532
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2036 2941 3503 4906 5280 5426
Size: 1064 138 447 905 562 1403 374 146
Cuts at: 5426 387
Size: 1280
Fragments arranged by size:
1403 1280 1064 905 562 447 374 146
138
EcoICRI GAG'CTC
Cuts at: 3477 3477
Size: 6319
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2052 2072 2261 2304 2400 2446 2526
Size: 393 420 20 189 43 96 46 80
Cuts at: 2526 2766 2881 2972 3560 4382 4979 5766 6034
Size: 240 115 91 588 822 597 787 268
Cuts at: 6034 1239
Size: 1524
Fragments arranged by size:
1524 822 787 597 588 420 393 268
240 189 115 96 91 80 46 43
20
EcoNI CCTnn'n_nnAGG
Cuts at: 2978 3247 2978
Size: 269 6050
EcoO109I rG'GnC_Cy
Cuts at: 2082 2083 2196 2197 2228 2249 2421 2511 2711
Size: 1 113 1 31 21 172 90 200
Cuts at: 2711 3586 2082
Size: 875 4815
Fragments arranged by size:
4815 875 200 172 113 90 31 21
1 1
EcoRI G'AATT_C
Cuts at: 3628 4006 3628
Size: 378 5941
EcoRV GAT'ATC
Cuts at: 3511 3511
Size: 6319
FspI TGC'GCA
Cuts at: 4825 4825
Size: 6319
HaeII r_GCGC'y
Cuts at: 1678 2800 5466 1678
Size: 1122 2666 2531
Fragments arranged by size:
2666 2531 1122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2471 2503 2750 2782 4960
Size: 32 135 32 899 32 247 32 2178
Cuts at: 4960 4992 5034 5066 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2178 899 247 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3663 3909 669
Size: 988 281 1725 246 3079
Fragments arranged by size:
3079 1725 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6319
HpaI GTT'AAC
Cuts at: 3909 3909
Size: 6319
KasI G'GCGC_C
Cuts at: 2796 2796
Size: 6319
KpnI G_GTAC'C
Cuts at: 2181 3644 2181
Size: 1463 4856
MfeI C'AATT_G
Cuts at: 3918 3918
Size: 6319
MluI A'CGCG_T
Cuts at: 6195 6195
Size: 6319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5433 5617 1305
Size: 4128 184 2007
Fragments arranged by size:
4128 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2345 3099 10
Size: 54 1425 343 513 754 3230
Fragments arranged by size:
3230 1425 754 513 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4277 4636 4795 518
Size: 648 3111 359 159 2042
Fragments arranged by size:
3111 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2551 3735 4412 5560 5805 1606
Size: 945 1184 677 1148 245 2120
Fragments arranged by size:
2120 1184 1148 945 677 245
NarI GG'CG_CC
Cuts at: 2797 2797
Size: 6319
NcoI C'CATG_G
Cuts at: 513 1065 2919 513
Size: 552 1854 3913
Fragments arranged by size:
3913 1854 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6319
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6319
NotI GC'GGCC_GC
Cuts at: 3766 3766
Size: 6319
NspI r_CATG'y
Cuts at: 1132 1229 3358 3677 5222 1132
Size: 97 2129 319 1545 2229
Fragments arranged by size:
2229 2129 1545 319 97
PasI CC'CwG_GG
Cuts at: 1348 2252 2515 2528 2809 1348
Size: 904 263 13 281 4858
Fragments arranged by size:
4858 904 281 263 13
PciI A'CATG_T
Cuts at: 5218 5218
Size: 6319
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2481 2612 3316 1108
Size: 1373 131 704 4111
Fragments arranged by size:
4111 1373 704 131
PmeI GTTT'AAAC
Cuts at: 3623 3623
Size: 6319
PmlI CAC'GTG
Cuts at: 2014 2014
Size: 6319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4388 4420 5901 5933 4388
Size: 32 1481 32 4774
Fragments arranged by size:
4774 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2228 2511 2228
Size: 283 6036
PshAI GACnn'nnGTC
Cuts at: 1613 2509 2913 1613
Size: 896 404 5019
Fragments arranged by size:
5019 896 404
PsiI TTA'TAA
Cuts at: 3889 3889
Size: 6319
PspOMI G'GGCC_C
Cuts at: 2082 2196 2879 2082
Size: 114 683 5522
Fragments arranged by size:
5522 683 114
PstI C_TGCA'G
Cuts at: 830 3573 3671 830
Size: 2743 98 3478
Fragments arranged by size:
3478 2743 98
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6319
PvuII CAG'CTG
Cuts at: 2551 2551
Size: 6319
SacI G_AGCT'C
Cuts at: 3479 3479
Size: 6319
SalI G'TCGA_C
Cuts at: 1936 3661 1936
Size: 1725 4594
SanDI GG'GwC_CC
Cuts at: 2228 2511 2228
Size: 283 6036
SbfI CC_TGCA'GG
Cuts at: 3671 3671
Size: 6319
ScaI AGT'ACT
Cuts at: 4567 6279 4567
Size: 1712 4607
SexAI A'CCwGG_T
Cuts at: 2148 2148
Size: 6319
SfcI C'TryA_G
Cuts at: 826 1045 2110 3569 3667 4802 5483 5674 826
Size: 219 1065 1459 98 1135 681 191 1471
Fragments arranged by size:
1471 1459 1135 1065 681 219 191 98
SfoI GGC'GCC
Cuts at: 2798 2798
Size: 6319
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6319
SmaI CCC'GGG
Cuts at: 1872 2140 2234 3646 1872
Size: 268 94 1412 4545
Fragments arranged by size:
4545 1412 268 94
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3493 4423 5324 5586 820
Size: 197 926 1550 930 901 262 1553
Fragments arranged by size:
1553 1550 930 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4110 493
Size: 3617 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6319
SphI G_CATG'C
Cuts at: 1132 3677 1132
Size: 2545 3774
SspI AAT'ATT
Cuts at: 5 52 4243 5
Size: 47 4191 2081
Fragments arranged by size:
4191 2081 47
StuI AGG'CCT
Cuts at: 1517 2991 1517
Size: 1474 4845
StyI C'CwwG_G
Cuts at: 513 1065 2642 2919 3243 3270 3758 513
Size: 552 1577 277 324 27 488 3074
Fragments arranged by size:
3074 1577 552 488 324 277 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3398 3449 3596 4346 4363 4516 4701
Size: 1255 1818 51 147 750 17 153 185
Cuts at: 4701 5146 325
Size: 445 1498
Fragments arranged by size:
1818 1498 1255 750 445 185 153 147
51 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4565 6277 96
Size: 274 80 33 51 1443 2588 1712 138
Fragments arranged by size:
2588 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2212 2244 2325 2357 6279 6311 2212
Size: 32 81 32 3922 32 2220
Fragments arranged by size:
3922 2220 81 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6319
XbaI T'CTAG_A
Cuts at: 3655 3655
Size: 6319
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2873 3313 2873
Size: 440 5879
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6319
XmaI C'CCGG_G
Cuts at: 1870 2138 2232 3644 1870
Size: 268 94 1412 4545
Fragments arranged by size:
4545 1412 268 94
XmnI GAAnn'nnTTC
Cuts at: 4448 4448
Size: 6319
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4127 276
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbvCI BfrBI BplI BsiWI
BstXI BstZ17I DraIII FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PspXI PsrI RsrII SacII
SapI SfiI SrfI SwaI