Restriction map for
pFN21AA1534
(Linear) (Six-base) MAPSORT of: KIAA1534.seq Check: 3766 from: 1 to: 2433
KIAA1534.orf
With 182 enzymes: SgfI *
February 20, 2009 16:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 559 1209 2433
Size: 559 650 1224
Fragments arranged by size:
1224 650 559
AccI GT'mk_AC
Cuts at: 0 68 2433
Size: 68 2365
Acc65I G'GTAC_C
Cuts at: 0 1091 2433
Size: 1091 1342
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 447 940 1969 2433
Size: 418 29 493 1029 464
Fragments arranged by size:
1029 493 464 418 29
AfeI AGC'GCT
Cuts at: 0 357 2433
Size: 357 2076
AflIII A'CryG_T
Cuts at: 0 1613 2433
Size: 1613 820
AleI CACnn'nnGTG
Cuts at: 0 208 823 2433
Size: 208 615 1610
Fragments arranged by size:
1610 615 208
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 658 690 988 1020 2433
Size: 658 32 298 32 1413
Fragments arranged by size:
1413 658 298 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 441 1689 1937 2247 2289 2433
Size: 441 1248 248 310 42 144
Fragments arranged by size:
1248 441 310 248 144 42
ApaI G_GGCC'C
Cuts at: 0 185 406 801 1839 2045 2433
Size: 185 221 395 1038 206 388
Fragments arranged by size:
1038 395 388 221 206 185
ApoI r'AATT_y
Cuts at: 0 1501 2433
Size: 1501 932
AvaI C'yCGr_G
Cuts at: 0 1551 1662 2433
Size: 1551 111 771
Fragments arranged by size:
1551 771 111
BamHI G'GATC_C
Cuts at: 0 1979 2131 2433
Size: 1979 152 302
Fragments arranged by size:
1979 302 152
BanI G'GyrC_C
Cuts at: 0 23 210 547 1091 1704 2297 2340 2433
Size: 23 187 337 544 613 593 43 93
Fragments arranged by size:
613 593 544 337 187 93 43 23
BanII G_rGCy'C
Cuts at: 0 119 152 185 406 427 801 1839 2045
Size: 119 33 33 221 21 374 1038 206
Cuts at: 2045 2159 2206 2320 2368 2433
Size: 114 47 114 48 65
Fragments arranged by size:
1038 374 221 206 119 114 114 65
48 47 33 33 21
BbeI G_GCGC'C
Cuts at: 0 27 2433
Size: 27 2406
BbsI GAAGACnn'nnnn_
Cuts at: 0 665 766 2433
Size: 665 101 1667
Fragments arranged by size:
1667 665 101
BbvCI CC'TCA_GC
Cuts at: 0 100 627 1690 2433
Size: 100 527 1063 743
Fragments arranged by size:
1063 743 527 100
BfrBI ATG'CAT
Cuts at: 0 691 2433
Size: 691 1742
BglI GCCn_nnn'nGGC
Cuts at: 0 401 1595 2433
Size: 401 1194 838
Fragments arranged by size:
1194 838 401
BglII A'GATC_T
Cuts at: 0 1547 2433
Size: 1547 886
BlpI GC'TnA_GC
Cuts at: 0 352 2314 2433
Size: 352 1962 119
Fragments arranged by size:
1962 352 119
Bme1580I G_kGCm'C
Cuts at: 0 185 406 550 801 1839 2045 2433
Size: 185 221 144 251 1038 206 388
Fragments arranged by size:
1038 388 251 221 206 185 144
BmgBI CAC'GTC
Cuts at: 0 1254 1565 1614 1755 2433
Size: 1254 311 49 141 678
Fragments arranged by size:
1254 678 311 141 49
BmrI ACTGGGnnnn_n'
Cuts at: 0 553 1611 1956 2433
Size: 553 1058 345 477
Fragments arranged by size:
1058 553 477 345
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1002 2433
Size: 1002 1431
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 700 997 1020 1462 1732 1750 1834
Size: 529 171 297 23 442 270 18 84
Cuts at: 1834 2433
Size: 599
Fragments arranged by size:
599 529 442 297 270 171 84 23
18
Bpu10I CC'TnA_GC
Cuts at: 0 100 627 1690 2433
Size: 100 527 1063 743
Fragments arranged by size:
1063 743 527 100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 1495 2433
Size: 280 1215 938
Fragments arranged by size:
1215 938 280
BsaI GGTCTCn'nnnn_
Cuts at: 0 733 781 1150 2433
Size: 733 48 369 1283
Fragments arranged by size:
1283 733 369 48
BsaHI Gr'CG_yC
Cuts at: 0 24 503 865 2433
Size: 24 479 362 1568
Fragments arranged by size:
1568 479 362 24
BsaWI w'CCGG_w
Cuts at: 0 1262 2433
Size: 1262 1171
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 868 898 988 1018 1048 1562 1592 1717
Size: 868 30 90 30 30 514 30 125
Cuts at: 1717 1747 2083 2113 2433
Size: 30 336 30 320
Fragments arranged by size:
868 514 336 320 125 90 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 88 739 856 904 1066 1729 1831
Size: 22 66 651 117 48 162 663 102
Cuts at: 1831 2182 2433
Size: 351 251
Fragments arranged by size:
663 651 351 251 162 117 102 66
48 22
BseYI C'CCAG_C
Cuts at: 0 370 445 846 855 1446 1493 2279 2433
Size: 370 75 401 9 591 47 786 154
Fragments arranged by size:
786 591 401 370 154 75 47 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2233 2238 2275 2433
Size: 2233 5 37 158
Fragments arranged by size:
2233 158 37 5
BsiHKAI G_wGCw'C
Cuts at: 0 119 2159 2320 2433
Size: 119 2040 161 113
Fragments arranged by size:
2040 161 119 113
BsmI GAATG_Cn'
Cuts at: 0 691 2433
Size: 691 1742
BsmBI CGTCTCn'nnnn_
Cuts at: 0 560 2433
Size: 560 1873
Bsp1286I G_dGCh'C
Cuts at: 0 119 152 185 406 427 550 801 1839
Size: 119 33 33 221 21 123 251 1038
Cuts at: 1839 2045 2159 2206 2320 2368 2433
Size: 206 114 47 114 48 65
Fragments arranged by size:
1038 251 221 206 123 119 114 114
65 48 47 33 33 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 559 822 1037 1209 2433
Size: 559 263 215 172 1224
Fragments arranged by size:
1224 559 263 215 172
BsrBI CCG'CTC
Cuts at: 0 1637 1667 1845 2433
Size: 1637 30 178 588
Fragments arranged by size:
1637 588 178 30
BsrFI r'CCGG_y
Cuts at: 0 216 317 2433
Size: 216 101 2116
Fragments arranged by size:
2116 216 101
BssHII G'CGCG_C
Cuts at: 0 333 2433
Size: 333 2100
BssSI C'ACGA_G
Cuts at: 0 2050 2251 2433
Size: 2050 201 182
Fragments arranged by size:
2050 201 182
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1704 2247 2289 2433
Size: 1704 543 42 144
Fragments arranged by size:
1704 543 144 42
BstEII G'GTnAC_C
Cuts at: 0 276 1458 2433
Size: 276 1182 975
Fragments arranged by size:
1182 975 276
BstXI CCAn_nnnn'nTGG
Cuts at: 0 825 2028 2433
Size: 825 1203 405
Fragments arranged by size:
1203 825 405
BstYI r'GATC_y
Cuts at: 0 1547 1979 2131 2433
Size: 1547 432 152 302
Fragments arranged by size:
1547 432 302 152
Bsu36I CC'TnA_GG
Cuts at: 0 722 2433
Size: 722 1711
BtgI C'CryG_G
Cuts at: 0 2006 2117 2321 2433
Size: 2006 111 204 112
Fragments arranged by size:
2006 204 112 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 590 1941 2433
Size: 590 1351 492
Fragments arranged by size:
1351 590 492
BtsI GCAGTG_nn'
Cuts at: 0 1407 1650 2433
Size: 1407 243 783
Fragments arranged by size:
1407 783 243
DraIII CAC_nnn'GTG
Cuts at: 0 1704 2433
Size: 1704 729
EaeI y'GGCC_r
Cuts at: 0 931 1596 2103 2306 2433
Size: 931 665 507 203 127
Fragments arranged by size:
931 665 507 203 127
EcoICRI GAG'CTC
Cuts at: 0 117 2157 2318 2433
Size: 117 2040 161 115
Fragments arranged by size:
2040 161 117 115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 447 529 700 940 997 1020 1462
Size: 418 29 82 171 240 57 23 442
Cuts at: 1462 1732 1750 1834 1969 2433
Size: 270 18 84 135 464
Fragments arranged by size:
464 442 418 270 240 171 135 84
82 57 29 23 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2251 2433
Size: 2251 182
EcoO109I rG'GnC_Cy
Cuts at: 0 181 182 402 403 581 797 798 1941
Size: 181 1 220 1 178 216 1 1143
Cuts at: 1941 2041 2244 2433
Size: 100 203 189
Fragments arranged by size:
1143 220 216 203 189 181 178 100
1 1 1
EcoRI G'AATT_C
Cuts at: 0 1501 2433
Size: 1501 932
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1290 1500 1536 2433
Size: 1290 210 36 897
Fragments arranged by size:
1290 897 210 36
HaeII r_GCGC'y
Cuts at: 0 27 359 1336 2433
Size: 27 332 977 1097
Fragments arranged by size:
1097 977 332 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 679 711 868 900 1018 1050 1207 1239
Size: 679 32 157 32 118 32 157 32
Cuts at: 1239 1562 1594 1717 1749 2083 2115 2433
Size: 323 32 123 32 334 32 318
Fragments arranged by size:
679 334 323 318 157 157 123 118
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 69 2433
Size: 69 2364
KasI G'GCGC_C
Cuts at: 0 23 2433
Size: 23 2410
KpnI G_GTAC'C
Cuts at: 0 1095 2433
Size: 1095 1338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 1031 2433
Size: 686 345 1402
Fragments arranged by size:
1402 686 345
MscI TGG'CCA
Cuts at: 0 1598 2433
Size: 1598 835
MslI CAynn'nnrTG
Cuts at: 0 208 823 2433
Size: 208 615 1610
Fragments arranged by size:
1610 615 208
MspA1I CmG'CkG
Cuts at: 0 104 348 1166 1497 1710 1827 1833 1878
Size: 104 244 818 331 213 117 6 45
Cuts at: 1878 2073 2129 2409 2433
Size: 195 56 280 24
Fragments arranged by size:
818 331 280 244 213 195 117 104
56 45 24 6
NarI GG'CG_CC
Cuts at: 0 24 2433
Size: 24 2409
NsiI A_TGCA'T
Cuts at: 0 693 2433
Size: 693 1740
PasI CC'CwG_GG
Cuts at: 0 133 170 793 1937 2038 2433
Size: 133 37 623 1144 101 395
Fragments arranged by size:
1144 623 395 133 101 37
PfoI T'CCnGG_A
Cuts at: 0 836 2433
Size: 836 1597
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 988 1020 2433
Size: 988 32 1413
Fragments arranged by size:
1413 988 32
PpuMI rG'GwC_Cy
Cuts at: 0 581 1941 2433
Size: 581 1360 492
Fragments arranged by size:
1360 581 492
PspOMI G'GGCC_C
Cuts at: 0 181 402 797 1835 2041 2433
Size: 181 221 395 1038 206 392
Fragments arranged by size:
1038 395 392 221 206 181
PstI C_TGCA'G
Cuts at: 0 2243 2363 2433
Size: 2243 120 70
Fragments arranged by size:
2243 120 70
PvuII CAG'CTG
Cuts at: 0 348 1497 1878 2409 2433
Size: 348 1149 381 531 24
Fragments arranged by size:
1149 531 381 348 24
SacI G_AGCT'C
Cuts at: 0 119 2159 2320 2433
Size: 119 2040 161 113
Fragments arranged by size:
2040 161 119 113
SalI G'TCGA_C
Cuts at: 0 67 2433
Size: 67 2366
SanDI GG'GwC_CC
Cuts at: 0 1941 2433
Size: 1941 492
SfcI C'TryA_G
Cuts at: 0 1062 2239 2359 2433
Size: 1062 1177 120 74
Fragments arranged by size:
1177 1062 120 74
SfoI GGC'GCC
Cuts at: 0 25 2433
Size: 25 2408
SmlI C'TyrA_G
Cuts at: 0 295 1510 2433
Size: 295 1215 923
Fragments arranged by size:
1215 923 295
StuI AGG'CCT
Cuts at: 0 13 2354 2433
Size: 13 2341 79
Fragments arranged by size:
2341 79 13
StyI C'CwwG_G
Cuts at: 0 1118 2433
Size: 1118 1315
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 656 1438 1915 2433
Size: 656 782 477 518
Fragments arranged by size:
782 656 518 477
TatI w'GTAC_w
Cuts at: 0 224 2433
Size: 224 2209
Tth111I GACn'n_nGTC
Cuts at: 0 882 2185 2433
Size: 882 1303 248
Fragments arranged by size:
1303 882 248
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 972 1044 2433
Size: 972 72 1389
Fragments arranged by size:
1389 972 72
XmnI GAAnn'nnTTC
Cuts at: 0 1483 2433
Size: 1483 950
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AleI AloI
AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrFI BssHII BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
KasI KpnI MmeI MscI MslI MspA1I NarI NsiI
PasI PfoI PpiI PpuMI PspOMI PstI PvuII SacI
SalI SanDI SfcI SfoI SmlI StuI StyI TaqII
TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AhdI AlfI ApaLI AscI
AseI AsiSI AvrII BaeI BcgI BciVI BclI BmtI
BsaAI BsaBI BsiEI BsiWI BspEI BspHI BsrDI BsrGI
BstBI BstZ17I ClaI CspCI DraI DrdI EagI EarI
EciI EcoRV FseI FspI FspAI HindIII HpaI MfeI
MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NspI PacI PciI PflMI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TstI XbaI XhoI XmaI ZraI