(Circular) (Six-base) MAPSORT of: pFN21AA1485.seq Check: 2165 from: 1 to: 8434 pFN21AA1485 8434 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2964 3014 4008 2964 Size: 50 994 7390 Fragments arranged by size: 7390 994 50 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3102 6244 278 Size: 53 83 186 936 1566 3142 2468 Fragments arranged by size: 3142 2468 1566 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5634 5670 5777 1937 Size: 3697 36 107 4594 Fragments arranged by size: 4594 3697 107 36 Acc65I G'GTAC_C Cuts at: 4260 5755 4260 Size: 1495 6939 AclI AA'CG_TT Cuts at: 1563 6561 6934 1563 Size: 4998 373 3063 Fragments arranged by size: 4998 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2691 4418 4676 5051 6497 7881 8149 1632 Size: 1059 1727 258 375 1446 1384 268 1917 Fragments arranged by size: 1917 1727 1446 1384 1059 375 268 258 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8237 AflIII A'CryG_T Cuts at: 1203 4824 7333 8310 1203 Size: 3621 2509 977 1327 Fragments arranged by size: 3621 2509 1327 977 AgeI A'CCGG_T Cuts at: 4128 7265 4128 Size: 3137 5297 AhdI GACnn_n'nnGTC Cuts at: 7163 7163 Size: 8434 AleI CACnn'nnGTG Cuts at: 1166 3228 4818 5387 1166 Size: 2062 1590 569 4213 Fragments arranged by size: 4213 2062 1590 569 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2435 3468 3734 1184 Size: 1251 1033 266 5884 Fragments arranged by size: 5884 1251 1033 266 AlwNI CAG_nnn'CTG Cuts at: 3343 4070 4948 5344 6177 7749 3343 Size: 727 878 396 833 1572 4028 Fragments arranged by size: 4028 1572 878 833 727 396 ApaI G_GGCC'C Cuts at: 2673 2673 Size: 8434 ApaLI G'TGCA_C Cuts at: 2951 3032 3047 3443 3683 4906 6491 7647 2951 Size: 81 15 396 240 1223 1585 1156 3738 Fragments arranged by size: 3738 1585 1223 1156 396 240 81 15 ApoI r'AATT_y Cuts at: 1493 2425 2692 4838 5743 5969 6121 8230 1493 Size: 932 267 2146 905 226 152 2109 1697 Fragments arranged by size: 2146 2109 1697 932 905 267 226 152 AseI AT'TA_AT Cuts at: 160 1032 6988 160 Size: 872 5956 1606 Fragments arranged by size: 5956 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8434 AvaI C'yCGr_G Cuts at: 1870 1943 4299 4598 5759 1870 Size: 73 2356 299 1161 4545 Fragments arranged by size: 4545 2356 1161 299 73 AvrII C'CTAG_G Cuts at: 3964 3964 Size: 8434 BamHI G'GATC_C Cuts at: 2877 4206 5764 6131 2877 Size: 1329 1558 367 5180 Fragments arranged by size: 5180 1558 1329 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2719 3950 4260 5081 5156 Size: 325 217 627 932 1231 310 821 75 Cuts at: 5156 5420 5467 5755 7210 618 Size: 264 47 288 1455 1842 Fragments arranged by size: 1842 1455 1231 932 821 627 325 310 288 264 217 75 47 BanII G_rGCy'C Cuts at: 1391 2673 3177 3312 3962 4711 5265 5514 5753 Size: 1282 504 135 650 749 554 249 239 Cuts at: 5753 1391 Size: 4072 Fragments arranged by size: 4072 1282 749 650 554 504 249 239 135 BbeI G_GCGC'C Cuts at: 3954 5424 3954 Size: 1470 6964 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2833 3463 5307 928 Size: 971 934 630 1844 4055 Fragments arranged by size: 4055 1844 971 934 630 BbvCI CC'TCA_GC Cuts at: 4041 4041 Size: 8434 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3385 3419 6625 6659 1903 Size: 34 1448 34 3206 34 3678 Fragments arranged by size: 3678 3206 1448 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6326 7536 6326 Size: 1210 7224 BclI T'GATC_A Cuts at: 1548 3894 1548 Size: 2346 6088 BfrBI ATG'CAT Cuts at: 4282 4282 Size: 8434 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2936 3461 7045 Size: 107 122 71 631 605 1264 525 3584 Cuts at: 7045 136 Size: 1525 Fragments arranged by size: 3584 1525 1264 631 605 525 122 107 71 BglII A'GATC_T Cuts at: 2553 8429 2553 Size: 5876 2558 BlpI GC'TnA_GC Cuts at: 5851 5851 Size: 8434 Bme1580I G_kGCm'C Cuts at: 1790 2673 2909 2955 3036 3051 3447 3687 4910 Size: 883 236 46 81 15 396 240 1223 Cuts at: 4910 4996 5084 6495 7651 1790 Size: 86 88 1411 1156 2573 Fragments arranged by size: 2573 1411 1223 1156 883 396 240 236 88 86 81 46 15 BmgBI CAC'GTC Cuts at: 1318 5608 1318 Size: 4290 4144 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2905 5266 6165 Size: 416 53 83 399 24 1488 2361 899 Cuts at: 6165 7123 442 Size: 958 1753 Fragments arranged by size: 2361 1753 1488 958 899 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8434 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2323 5281 5533 2323 Size: 2958 252 5224 Fragments arranged by size: 5224 2958 252 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2991 3563 3650 4047 4250 4416 5321 7094 Size: 1752 572 87 397 203 166 905 1773 Cuts at: 7094 1239 Size: 2579 Fragments arranged by size: 2579 1773 1752 905 572 397 203 166 87 Bpu10I CC'TnA_GC Cuts at: 3011 4041 4122 3011 Size: 1030 81 7323 Fragments arranged by size: 7323 1030 81 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2167 3011 3581 3756 4760 6559 7424 7722 2167 Size: 844 570 175 1004 1799 865 298 2879 Fragments arranged by size: 2879 1799 1004 865 844 570 298 175 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2693 3754 4218 4576 5217 5420 7097 Size: 617 1194 1061 464 358 641 203 1677 Cuts at: 7097 882 Size: 2219 Fragments arranged by size: 2219 1677 1194 1061 641 617 464 358 203 BsaAI yAC'GTr Cuts at: 493 1204 4369 5674 6225 493 Size: 711 3165 1305 551 2702 Fragments arranged by size: 3165 2702 1305 711 551 BsaBI GATnn'nnATC Cuts at: 8428 8428 Size: 8434 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3099 3951 5421 5436 Size: 53 83 186 936 1566 852 1470 15 Cuts at: 5436 6241 6623 7258 275 Size: 805 382 635 1451 Fragments arranged by size: 1566 1470 1451 936 852 805 635 382 186 83 53 15 BsaWI w'CCGG_w Cuts at: 2192 2317 4128 4209 6867 7265 7539 7686 8097 Size: 125 1811 81 2658 398 274 147 411 Cuts at: 8097 2192 Size: 2529 Fragments arranged by size: 2658 2529 1811 411 398 274 147 125 81 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3677 3707 5236 5266 1178 Size: 30 165 30 2274 30 1529 30 4346 Fragments arranged by size: 4346 2274 1529 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 2282 2450 3581 3647 3742 3745 3805 Size: 889 207 168 1131 66 95 3 60 Cuts at: 3805 4046 4618 5078 5273 5519 5522 5714 1186 Size: 241 572 460 195 246 3 192 3906 Fragments arranged by size: 3906 1131 889 572 460 246 241 207 195 192 168 95 66 60 3 3 BseYI C'CCAG_C Cuts at: 2666 3676 3926 4013 4036 5416 5513 7637 8133 Size: 1010 250 87 23 1380 97 2124 496 Cuts at: 8133 2666 Size: 2967 Fragments arranged by size: 2967 2124 1380 1010 496 250 97 87 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2604 2935 5696 1163 Size: 573 868 331 2761 3901 Fragments arranged by size: 3901 2761 868 573 331 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5371 5884 6645 7673 1540 Size: 275 186 3370 513 761 1028 2301 Fragments arranged by size: 3370 2301 1028 761 513 275 186 BsiHKAI G_wGCw'C Cuts at: 2955 3036 3051 3177 3312 3447 3687 4128 4583 Size: 81 15 126 135 135 240 441 455 Cuts at: 4583 4910 5109 6495 6580 7651 2955 Size: 327 199 1386 85 1071 3738 Fragments arranged by size: 3738 1386 1071 455 441 327 240 199 135 135 126 85 81 15 BsmI GAATG_Cn' Cuts at: 3595 3766 5945 6038 3595 Size: 171 2179 93 5991 Fragments arranged by size: 5991 2179 171 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2003 3120 5537 5615 2003 Size: 1117 2417 78 4822 Fragments arranged by size: 4822 2417 1117 78 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2673 2909 2955 3036 3051 3177 3312 Size: 399 883 236 46 81 15 126 135 Cuts at: 3312 3447 3687 3962 4128 4583 4711 4910 4996 Size: 135 240 275 166 455 128 199 86 Cuts at: 4996 5084 5109 5265 5514 5753 6495 6580 7651 Size: 88 25 156 249 239 742 85 1071 Cuts at: 7651 1391 Size: 2174 Fragments arranged by size: 2174 1071 883 742 455 399 275 249 240 239 236 199 166 156 135 135 128 126 88 86 85 81 46 25 15 BspEI T'CCGG_A Cuts at: 2317 4209 8097 2317 Size: 1892 3888 2654 Fragments arranged by size: 3888 2654 1892 BspHI T'CATG_A Cuts at: 2211 4149 4671 4711 5532 6323 8053 2211 Size: 1938 522 40 821 791 1730 2592 Fragments arranged by size: 2592 1938 1730 821 791 522 40 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2441 2964 3014 3771 4008 5485 5789 844 Size: 1597 523 50 757 237 1477 304 3489 Fragments arranged by size: 3489 1597 1477 757 523 304 237 50 BsrBI CCG'CTC Cuts at: 1394 1823 4204 6321 1394 Size: 429 2381 2117 3507 Fragments arranged by size: 3507 2381 2117 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4996 6929 7103 65 Size: 1183 3748 1933 174 1396 Fragments arranged by size: 3748 1933 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4128 5719 7078 7265 1700 Size: 110 2318 1591 1359 187 2869 Fragments arranged by size: 2869 2318 1591 1359 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8434 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8434 BssSI C'ACGA_G Cuts at: 6494 7506 6494 Size: 1012 7422 BstAPI GCAn_nnn'nTGC Cuts at: 794 2860 794 Size: 2066 6368 BstBI TT'CG_AA Cuts at: 7238 7238 Size: 8434 BstEII G'GTnAC_C Cuts at: 1184 4001 5497 1184 Size: 2817 1496 4121 Fragments arranged by size: 4121 2817 1496 BstXI CCAn_nnnn'nTGG Cuts at: 3926 5120 3926 Size: 1194 7240 BstYI r'GATC_y Cuts at: 1972 2553 2877 4009 4206 5764 6131 6516 6533 Size: 581 324 1132 197 1558 367 385 17 Cuts at: 6533 7985 8071 8083 8429 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1558 1452 1132 581 385 367 346 324 197 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2150 2235 2873 4566 5256 2150 Size: 85 638 1693 690 5328 Fragments arranged by size: 5328 1693 690 638 85 BtgI C'CryG_G Cuts at: 513 1065 2004 2522 3280 3319 3744 3946 5382 Size: 552 939 518 758 39 425 202 1436 Cuts at: 5382 5463 5562 513 Size: 81 99 3385 Fragments arranged by size: 3385 1436 939 758 552 518 425 202 99 81 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7695 Fragments arranged by size: 7695 683 56 BtsI GCAGTG_nn' Cuts at: 3067 4881 5439 5950 6744 6764 3067 Size: 1814 558 511 794 20 4737 Fragments arranged by size: 4737 1814 794 558 511 20 ClaI AT'CG_AT Cuts at: 1554 4531 1554 Size: 2977 5457 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5428 5463 529 Size: 35 4864 35 3500 Fragments arranged by size: 4864 3500 35 35 DraI TTT'AAA Cuts at: 5738 5738 Size: 8434 DraIII CAC_nnn'GTG Cuts at: 3728 4914 5590 3728 Size: 1186 676 6572 Fragments arranged by size: 6572 1186 676 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3152 7441 809 Size: 507 1836 4289 1802 Fragments arranged by size: 4289 1836 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 3316 5328 Size: 54 1425 50 275 18 201 1285 2012 Cuts at: 5328 5881 6770 8399 8 Size: 553 889 1629 43 Fragments arranged by size: 2012 1629 1425 1285 889 553 275 201 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5881 1537 Size: 275 4069 4090 Fragments arranged by size: 4090 4069 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2066 2210 2216 2259 3558 3597 6364 1353 Size: 713 144 6 43 1299 39 2767 3423 Fragments arranged by size: 3423 2767 1299 713 144 43 39 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7021 7395 7541 387 Size: 1064 138 5432 374 146 1280 Fragments arranged by size: 5432 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3175 3310 3175 Size: 135 8299 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2691 2991 3563 3650 4047 4250 4416 Size: 393 1059 300 572 87 397 203 166 Cuts at: 4416 4418 4676 5051 5321 6497 7094 7881 8149 Size: 2 258 375 270 1176 597 787 268 Cuts at: 8149 1239 Size: 1524 Fragments arranged by size: 1524 1176 1059 787 597 572 397 393 375 300 270 268 258 203 166 87 2 EcoNI CCTnn'n_nnAGG Cuts at: 2679 2727 3014 2679 Size: 48 287 8099 Fragments arranged by size: 8099 287 48 EcoO109I rG'GnC_Cy Cuts at: 2670 2795 3655 4156 5341 2670 Size: 125 860 501 1185 5763 Fragments arranged by size: 5763 1185 860 501 125 EcoRI G'AATT_C Cuts at: 5743 6121 5743 Size: 378 8056 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3385 3385 Size: 8434 FspI TGC'GCA Cuts at: 2960 3425 6940 2960 Size: 465 3515 4454 Fragments arranged by size: 4454 3515 465 HaeII r_GCGC'y Cuts at: 1678 3344 3954 5424 7581 1678 Size: 1666 610 1470 2157 2531 Fragments arranged by size: 2531 2157 1666 1470 610 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2981 3013 3800 3832 4115 Size: 32 135 32 1409 32 787 32 283 Cuts at: 4115 4147 4546 4578 7075 7107 7149 7181 1373 Size: 32 399 32 2497 32 42 32 2626 Fragments arranged by size: 2626 2497 1409 787 399 283 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5778 6024 669 Size: 988 281 3840 246 3079 Fragments arranged by size: 3840 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8434 HpaI GTT'AAC Cuts at: 6024 6024 Size: 8434 KasI G'GCGC_C Cuts at: 3950 5420 3950 Size: 1470 6964 KpnI G_GTAC'C Cuts at: 4264 5759 4264 Size: 1495 6939 MfeI C'AATT_G Cuts at: 6033 6033 Size: 8434 MluI A'CGCG_T Cuts at: 8310 8310 Size: 8434 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2058 2318 2351 2406 5544 5625 7548 7732 Size: 753 260 33 55 3138 81 1923 184 Cuts at: 7732 1305 Size: 2007 Fragments arranged by size: 3138 2007 1923 753 260 184 81 55 33 MscI TGG'CCA Cuts at: 10 64 1489 1832 3318 10 Size: 54 1425 343 1486 5126 Fragments arranged by size: 5126 1486 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2039 2417 2606 2886 2902 3228 3986 Size: 648 873 378 189 280 16 326 758 Cuts at: 3986 4716 4818 5058 5387 5531 6392 6751 6910 Size: 730 102 240 329 144 861 359 159 Cuts at: 6910 518 Size: 2042 Fragments arranged by size: 2042 873 861 758 730 648 378 359 329 326 280 240 189 159 144 102 16 MspA1I CmG'CkG Cuts at: 1606 2395 3208 3556 3628 3680 3998 4040 4615 Size: 789 813 348 72 52 318 42 575 Cuts at: 4615 4928 4945 4989 5099 5324 5850 6527 7675 Size: 313 17 44 110 225 526 677 1148 Cuts at: 7675 7920 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 813 789 677 575 526 348 318 313 245 225 110 72 52 44 42 17 NaeI GCC'GGC Cuts at: 5721 5721 Size: 8434 NarI GG'CG_CC Cuts at: 3951 5421 3951 Size: 1470 6964 NcoI C'CATG_G Cuts at: 513 1065 2004 3319 3946 5463 513 Size: 552 939 1315 627 1517 3484 Fragments arranged by size: 3484 1517 1315 939 627 552 NdeI CA'TA_TG Cuts at: 387 3270 4785 387 Size: 2883 1515 4036 Fragments arranged by size: 4036 2883 1515 NgoMIV G'CCGG_C Cuts at: 5719 5719 Size: 8434 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8434 NotI GC'GGCC_GC Cuts at: 5881 5881 Size: 8434 NsiI A_TGCA'T Cuts at: 4284 4284 Size: 8434 NspI r_CATG'y Cuts at: 1132 1229 3356 3859 4286 4828 4970 5792 7337 Size: 97 2127 503 427 542 142 822 1545 Cuts at: 7337 1132 Size: 2229 Fragments arranged by size: 2229 2127 1545 822 542 503 427 142 97 PasI CC'CwG_GG Cuts at: 1348 3659 1348 Size: 2311 6123 PciI A'CATG_T Cuts at: 4824 7333 4824 Size: 2509 5925 PflMI CCAn_nnn'nTGG Cuts at: 1108 3274 3343 4070 5590 1108 Size: 2166 69 727 1520 3952 Fragments arranged by size: 3952 2166 1520 727 69 PfoI T'CCnGG_A Cuts at: 3504 5337 5538 3504 Size: 1833 201 6400 Fragments arranged by size: 6400 1833 201 PmeI GTTT'AAAC Cuts at: 5738 5738 Size: 8434 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3224 3256 6503 6535 8016 8048 3224 Size: 32 3247 32 1481 32 3610 Fragments arranged by size: 3610 3247 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2795 4156 5341 2795 Size: 1361 1185 5888 Fragments arranged by size: 5888 1361 1185 PshAI GACnn'nnGTC Cuts at: 1613 2524 1613 Size: 911 7523 PsiI TTA'TAA Cuts at: 6004 6004 Size: 8434 PspOMI G'GGCC_C Cuts at: 2669 2669 Size: 8434 PstI C_TGCA'G Cuts at: 830 2865 4888 5403 5786 830 Size: 2035 2023 515 383 3478 Fragments arranged by size: 3478 2035 2023 515 383 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8434 PvuII CAG'CTG Cuts at: 2395 3208 3556 3628 3680 3998 4040 4615 4945 Size: 813 348 72 52 318 42 575 330 Cuts at: 4945 2395 Size: 5884 Fragments arranged by size: 5884 813 575 348 330 318 72 52 42 SacI G_AGCT'C Cuts at: 3177 3312 3177 Size: 135 8299 SalI G'TCGA_C Cuts at: 1936 5776 1936 Size: 3840 4594 SbfI CC_TGCA'GG Cuts at: 5786 5786 Size: 8434 ScaI AGT'ACT Cuts at: 3092 6682 8394 3092 Size: 3590 1712 3132 Fragments arranged by size: 3590 3132 1712 SexAI A'CCwGG_T Cuts at: 2715 3511 5586 2715 Size: 796 2075 5563 Fragments arranged by size: 5563 2075 796 SfcI C'TryA_G Cuts at: 826 1045 2861 3551 4448 4884 5399 5782 6917 Size: 219 1816 690 897 436 515 383 1135 Cuts at: 6917 7598 7789 826 Size: 681 191 1471 Fragments arranged by size: 1816 1471 1135 897 690 681 515 436 383 219 191 SfoI GGC'GCC Cuts at: 3952 5422 3952 Size: 1470 6964 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8434 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8434 SmaI CCC'GGG Cuts at: 1872 4301 5761 1872 Size: 2429 1460 4545 Fragments arranged by size: 4545 2429 1460 SmlI C'TyrA_G Cuts at: 820 1017 1943 2182 3026 3596 3771 4775 6538 Size: 197 926 239 844 570 175 1004 1763 Cuts at: 6538 7439 7701 820 Size: 901 262 1553 Fragments arranged by size: 1763 1553 1004 926 901 844 570 262 239 197 175 SnaBI TAC'GTA Cuts at: 493 6225 493 Size: 5732 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8434 SphI G_CATG'C Cuts at: 1132 5792 1132 Size: 4660 3774 SspI AAT'ATT Cuts at: 5 52 6358 5 Size: 47 6306 2081 Fragments arranged by size: 6306 2081 47 StuI AGG'CCT Cuts at: 1517 2155 2233 3164 3464 3608 4046 5654 1517 Size: 638 78 931 300 144 438 1608 4297 Fragments arranged by size: 4297 1608 931 638 438 300 144 78 StyI C'CwwG_G Cuts at: 513 1065 2004 3165 3319 3465 3912 3946 3964 Size: 552 939 1161 154 146 447 34 18 Cuts at: 3964 5463 5873 513 Size: 1499 410 3074 Fragments arranged by size: 3074 1499 1161 939 552 447 410 154 146 34 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2420 3443 4307 5285 5385 6461 6478 Size: 1255 840 1023 864 978 100 1076 17 Cuts at: 6478 6631 6816 7261 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1076 1023 978 864 840 445 185 153 100 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2162 3090 6680 Size: 274 80 33 51 1443 185 928 3590 Cuts at: 6680 8392 96 Size: 1712 138 Fragments arranged by size: 3590 1712 1443 928 274 185 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3677 3709 8394 8426 3677 Size: 32 4685 32 3685 Fragments arranged by size: 4685 3685 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8434 XbaI T'CTAG_A Cuts at: 5770 5770 Size: 8434 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2605 5692 2605 Size: 3087 5347 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8434 XmaI C'CCGG_G Cuts at: 1870 4299 5759 1870 Size: 2429 1460 4545 Fragments arranged by size: 4545 2429 1460 XmnI GAAnn'nnTTC Cuts at: 2631 4727 5304 6563 2631 Size: 2096 577 1259 4502 Fragments arranged by size: 4502 2096 1259 577 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3100 6242 276 Size: 53 83 186 936 1566 3142 2468 Fragments arranged by size: 3142 2468 1566 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BsiWI BstZ17I EcoRV FseI FspAI NruI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI