(Circular) (Six-base) MAPSORT of: pFN21AA1405.seq Check: 9514 from: 1 to: 7792 pFN21AA1405 7792 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3133 3154 3388 3777 4213 4582 3133 Size: 21 234 389 436 369 6343 Fragments arranged by size: 6343 436 389 369 234 21 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3345 3924 5602 278 Size: 53 83 186 936 1809 579 1678 2468 Fragments arranged by size: 2468 1809 1678 936 579 186 83 53 AccI GT'mk_AC Cuts at: 1937 4404 5135 1937 Size: 2467 731 4594 Fragments arranged by size: 4594 2467 731 Acc65I G'GTAC_C Cuts at: 5113 5113 Size: 7792 AclI AA'CG_TT Cuts at: 1563 5919 6292 1563 Size: 4356 373 3063 Fragments arranged by size: 4356 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2507 4313 5855 7239 7507 1632 Size: 875 1806 1542 1384 268 1917 Fragments arranged by size: 1917 1806 1542 1384 875 268 AfeI AGC'GCT Cuts at: 3454 4319 3454 Size: 865 6927 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7595 AflIII A'CryG_T Cuts at: 1203 2364 6691 7668 1203 Size: 1161 4327 977 1327 Fragments arranged by size: 4327 1327 1161 977 AgeI A'CCGG_T Cuts at: 6623 6623 Size: 7792 AhdI GACnn_n'nnGTC Cuts at: 6521 6521 Size: 7792 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7792 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3963 1184 Size: 2779 5013 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2575 2606 2607 2638 2575 Size: 31 1 31 7729 Fragments arranged by size: 7729 31 31 1 AlwNI CAG_nnn'CTG Cuts at: 2314 2479 3158 3389 4076 4228 4585 4603 4617 Size: 165 679 231 687 152 357 18 14 Cuts at: 4617 5535 7107 2314 Size: 918 1572 2999 Fragments arranged by size: 2999 1572 918 687 679 357 231 165 152 18 14 ApaLI G'TGCA_C Cuts at: 2540 5849 7005 2540 Size: 3309 1156 3327 Fragments arranged by size: 3327 3309 1156 ApoI r'AATT_y Cuts at: 1493 5101 5327 5479 7588 1493 Size: 3608 226 152 2109 1697 Fragments arranged by size: 3608 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6346 160 Size: 872 5314 1606 Fragments arranged by size: 5314 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7792 AvaI C'yCGr_G Cuts at: 1870 1943 2128 3294 3746 5018 5117 1870 Size: 73 185 1166 452 1272 99 4545 Fragments arranged by size: 4545 1272 1166 452 185 99 73 BamHI G'GATC_C Cuts at: 2320 5122 5489 2320 Size: 2802 367 4623 Fragments arranged by size: 4623 2802 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2132 2171 2258 3145 3765 Size: 325 217 627 345 39 87 887 620 Cuts at: 3765 3814 5113 6568 618 Size: 49 1299 1455 1842 Fragments arranged by size: 1842 1455 1299 887 627 620 345 325 217 87 49 39 BanII G_rGCy'C Cuts at: 1391 3113 3617 3681 3972 4131 5111 1391 Size: 1722 504 64 291 159 980 4072 Fragments arranged by size: 4072 1722 980 504 291 159 64 BbeI G_GCGC'C Cuts at: 2136 2175 2136 Size: 39 7753 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3318 3496 928 Size: 971 1419 178 5224 Fragments arranged by size: 5224 1419 971 178 BbvCI CC'TCA_GC Cuts at: 3420 4889 4940 3420 Size: 1469 51 6272 Fragments arranged by size: 6272 1469 51 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2648 2682 3275 3309 3943 3977 5983 Size: 34 711 34 593 34 634 34 2006 Cuts at: 5983 6017 1903 Size: 34 3678 Fragments arranged by size: 3678 2006 711 634 593 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5684 6894 5684 Size: 1210 6582 BclI T'GATC_A Cuts at: 1548 1548 Size: 7792 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2015 2283 2996 Size: 107 122 71 631 605 343 268 713 Cuts at: 2996 3872 3998 4106 4115 6403 136 Size: 876 126 108 9 2288 1525 Fragments arranged by size: 2288 1525 876 713 631 605 343 268 126 122 108 107 71 9 BglII A'GATC_T Cuts at: 2202 2427 4030 4495 7787 2202 Size: 225 1603 465 3292 2207 Fragments arranged by size: 3292 2207 1603 465 225 BlpI GC'TnA_GC Cuts at: 4119 5209 4119 Size: 1090 6702 Bme1580I G_kGCm'C Cuts at: 1790 2544 3150 3768 3819 5853 7009 1790 Size: 754 606 618 51 2034 1156 2573 Fragments arranged by size: 2573 2034 1156 754 618 606 51 BmgBI CAC'GTC Cuts at: 1318 2191 2857 1318 Size: 873 666 6253 Fragments arranged by size: 6253 873 666 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2104 2548 2822 Size: 416 53 83 399 24 687 444 274 Cuts at: 2822 2912 3522 3966 4396 4715 4746 5523 6481 Size: 90 610 444 430 319 31 777 958 Cuts at: 6481 442 Size: 1753 Fragments arranged by size: 1753 958 777 687 610 444 444 430 416 399 319 274 90 83 53 31 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7792 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3646 3697 4579 3646 Size: 51 882 6859 Fragments arranged by size: 6859 882 51 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2444 2501 3326 3380 3544 3601 3986 4469 Size: 1205 57 825 54 164 57 385 483 Cuts at: 4469 4538 4607 4655 6452 1239 Size: 69 69 48 1797 2579 Fragments arranged by size: 2579 1797 1205 825 483 385 164 69 69 57 57 54 48 Bpu10I CC'TnA_GC Cuts at: 3420 4095 4125 4889 4940 3420 Size: 675 30 764 51 6272 Fragments arranged by size: 6272 764 675 51 30 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2759 3068 5917 6782 7080 2759 Size: 309 2849 865 298 3471 Fragments arranged by size: 3471 2849 865 309 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3700 6455 882 Size: 617 2201 2755 2219 Fragments arranged by size: 2755 2219 2201 617 BsaAI yAC'GTr Cuts at: 493 1204 2182 2365 2515 5583 493 Size: 711 978 183 150 3068 2702 Fragments arranged by size: 3068 2702 978 711 183 150 BsaBI GATnn'nnATC Cuts at: 7786 7786 Size: 7792 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2133 2172 2238 3342 Size: 53 83 186 936 600 39 66 1104 Cuts at: 3342 3921 4899 5599 5981 6616 275 Size: 579 978 700 382 635 1451 Fragments arranged by size: 1451 1104 978 936 700 635 600 579 382 186 83 66 53 39 BsaWI w'CCGG_w Cuts at: 6225 6623 6897 7044 7455 6225 Size: 398 274 147 411 6562 Fragments arranged by size: 6562 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3164 3194 3524 3554 4822 Size: 30 165 30 1761 30 330 30 1268 Cuts at: 4822 4852 1178 Size: 30 4118 Fragments arranged by size: 4118 1761 1268 330 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3083 3185 3251 3377 3689 3725 3815 3874 Size: 1897 102 66 126 312 36 90 59 Cuts at: 3874 4814 1186 Size: 940 4164 Fragments arranged by size: 4164 1897 940 312 126 102 90 66 59 36 BseYI C'CCAG_C Cuts at: 3113 3761 3772 4659 4998 6995 7491 3113 Size: 648 11 887 339 1997 496 3414 Fragments arranged by size: 3414 1997 887 648 496 339 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2406 3182 3959 4616 1163 Size: 573 670 776 777 657 4339 Fragments arranged by size: 4339 777 776 670 657 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2598 5242 6003 7031 1540 Size: 275 186 597 2644 761 1028 2301 Fragments arranged by size: 2644 2301 1028 761 597 275 186 BsiHKAI G_wGCw'C Cuts at: 2364 2496 2544 2565 5853 5938 7009 2364 Size: 132 48 21 3288 85 1071 3147 Fragments arranged by size: 3288 3147 1071 132 85 48 21 BsmI GAATG_Cn' Cuts at: 5303 5396 5303 Size: 93 7699 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2364 2496 2544 2565 3113 3150 3617 Size: 399 574 132 48 21 548 37 467 Cuts at: 3617 3681 3768 3819 3972 4131 5111 5853 5938 Size: 64 87 51 153 159 980 742 85 Cuts at: 5938 7009 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 980 742 574 548 467 399 159 153 132 87 85 64 51 48 37 21 BspEI T'CCGG_A Cuts at: 7455 7455 Size: 7792 BspHI T'CATG_A Cuts at: 5681 7411 5681 Size: 1730 6062 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3133 3154 3378 3388 3777 3931 3969 4213 Size: 2289 21 224 10 389 154 38 244 Cuts at: 4213 4246 4582 5147 844 Size: 33 336 565 3489 Fragments arranged by size: 3489 2289 565 389 336 244 224 154 38 33 21 10 BsrBI CCG'CTC Cuts at: 1394 1823 2680 2740 2770 5679 1394 Size: 429 857 60 30 2909 3507 Fragments arranged by size: 3507 2909 857 429 60 30 BsrDI GCAATG_nn' Cuts at: 65 1248 2824 4845 6287 6461 65 Size: 1183 1576 2021 1442 174 1396 Fragments arranged by size: 2021 1576 1442 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4135 4548 6436 6623 1700 Size: 110 2325 413 1888 187 2869 Fragments arranged by size: 2869 2325 1888 413 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7792 BssHII G'CGCG_C Cuts at: 1925 2100 2102 2104 1925 Size: 175 2 2 7613 Fragments arranged by size: 7613 175 2 2 BssSI C'ACGA_G Cuts at: 5852 6864 5852 Size: 1012 6780 BstAPI GCAn_nnn'nTGC Cuts at: 794 2314 2539 3814 4225 794 Size: 1520 225 1275 411 4361 Fragments arranged by size: 4361 1520 1275 411 225 BstBI TT'CG_AA Cuts at: 6596 6596 Size: 7792 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7792 BstYI r'GATC_y Cuts at: 1972 2202 2320 2427 4030 4495 4686 4884 5122 Size: 230 118 107 1603 465 191 198 238 Cuts at: 5122 5489 5874 5891 7343 7429 7441 7787 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1603 1452 465 385 367 346 238 230 198 191 118 107 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3672 4023 3672 Size: 351 7441 BtgI C'CryG_G Cuts at: 513 1065 2004 2082 2670 2760 3891 513 Size: 552 939 78 588 90 1131 4414 Fragments arranged by size: 4414 1131 939 588 552 90 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7053 Fragments arranged by size: 7053 683 56 BtsI GCAGTG_nn' Cuts at: 3318 4188 5086 5308 6102 6122 3318 Size: 870 898 222 794 20 4988 Fragments arranged by size: 4988 898 870 794 222 20 ClaI AT'CG_AT Cuts at: 1554 4527 1554 Size: 2973 4819 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7757 DraI TTT'AAA Cuts at: 5096 5096 Size: 7792 DraIII CAC_nnn'GTG Cuts at: 2539 2548 2539 Size: 9 7783 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6799 809 Size: 507 5483 1802 Fragments arranged by size: 5483 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2275 3873 3894 Size: 54 1425 50 275 18 445 1598 21 Cuts at: 3894 4137 4536 5239 6128 7757 8 Size: 243 399 703 889 1629 43 Fragments arranged by size: 1629 1598 1425 889 703 445 399 275 243 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 5239 1537 Size: 275 3427 4090 Fragments arranged by size: 4090 3427 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3240 4148 4901 5722 1353 Size: 1887 908 753 821 3423 Fragments arranged by size: 3423 1887 908 821 753 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2313 4535 5003 6379 6753 6899 Size: 1064 138 724 2222 468 1376 374 146 Cuts at: 6899 387 Size: 1280 Fragments arranged by size: 2222 1376 1280 1064 724 468 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2444 2501 2507 3326 3380 3544 3601 Size: 393 812 57 6 819 54 164 57 Cuts at: 3601 3986 4313 4469 4538 4607 4655 5855 6452 Size: 385 327 156 69 69 48 1200 597 Cuts at: 6452 7239 7507 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1200 819 812 787 597 393 385 327 268 164 156 69 69 57 57 54 48 6 EcoNI CCTnn'n_nnAGG Cuts at: 3374 3413 3749 4589 3374 Size: 39 336 840 6577 Fragments arranged by size: 6577 840 336 39 EcoO109I rG'GnC_Cy Cuts at: 2311 2352 2449 3034 3844 3931 3943 4099 4288 Size: 41 97 585 810 87 12 156 189 Cuts at: 4288 4984 2311 Size: 696 5119 Fragments arranged by size: 5119 810 696 585 189 156 97 87 41 12 EcoRI G'AATT_C Cuts at: 5101 5479 5101 Size: 378 7414 FspI TGC'GCA Cuts at: 3329 6298 3329 Size: 2969 4823 HaeII r_GCGC'y Cuts at: 1678 2136 2175 2834 2848 3253 3456 4321 6939 Size: 458 39 659 14 405 203 865 2618 Cuts at: 6939 1678 Size: 2531 Fragments arranged by size: 2618 2531 865 659 458 405 203 39 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2206 2238 2312 2328 2344 Size: 32 135 32 634 32 74 16 16 Cuts at: 2344 2360 2729 2761 3275 3307 3413 3445 3505 Size: 16 369 32 514 32 106 32 60 Cuts at: 3505 3524 3537 3556 4022 4054 4480 4487 4512 Size: 19 13 19 466 32 426 7 25 Cuts at: 4512 4519 4678 4710 6433 6465 6507 6539 1373 Size: 7 159 32 1723 32 42 32 2626 Fragments arranged by size: 2626 1723 634 514 466 426 369 159 135 106 74 60 42 32 32 32 32 32 32 32 32 32 32 25 19 19 16 16 16 13 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 5136 5382 669 Size: 988 281 3198 246 3079 Fragments arranged by size: 3198 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 4466 748 Size: 3718 4074 HpaI GTT'AAC Cuts at: 5382 5382 Size: 7792 KasI G'GCGC_C Cuts at: 2132 2171 2132 Size: 39 7753 KpnI G_GTAC'C Cuts at: 5117 5117 Size: 7792 MfeI C'AATT_G Cuts at: 5391 5391 Size: 7792 MluI A'CGCG_T Cuts at: 7668 7668 Size: 7792 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3708 4068 4227 4808 6906 7090 1305 Size: 2403 360 159 581 2098 184 2007 Fragments arranged by size: 2403 2098 2007 581 360 184 159 MscI TGG'CCA Cuts at: 10 64 1489 1832 3875 3896 4538 10 Size: 54 1425 343 2043 21 642 3264 Fragments arranged by size: 3264 2043 1425 642 343 54 21 MslI CAynn'nnrTG Cuts at: 518 1166 2609 2660 3323 4175 5750 6109 6268 Size: 648 1443 51 663 852 1575 359 159 Cuts at: 6268 518 Size: 2042 Fragments arranged by size: 2042 1575 1443 852 663 648 359 159 51 MspA1I CmG'CkG Cuts at: 1606 2056 2227 2289 3226 3761 4145 4201 4243 Size: 450 171 62 937 535 384 56 42 Cuts at: 4243 4345 4380 4516 4944 5208 5885 7033 7278 Size: 102 35 136 428 264 677 1148 245 Cuts at: 7278 1606 Size: 2120 Fragments arranged by size: 2120 1148 937 677 535 450 428 384 264 245 171 136 102 62 56 42 35 NaeI GCC'GGC Cuts at: 4137 4550 4137 Size: 413 7379 NarI GG'CG_CC Cuts at: 2133 2172 2133 Size: 39 7753 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6301 Fragments arranged by size: 6301 939 552 NdeI CA'TA_TG Cuts at: 387 3337 387 Size: 2950 4842 NgoMIV G'CCGG_C Cuts at: 4135 4548 4135 Size: 413 7379 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7792 NotI GC'GGCC_GC Cuts at: 5239 5239 Size: 7792 NruI TCG'CGA Cuts at: 3057 3057 Size: 7792 NspI r_CATG'y Cuts at: 1132 1229 5150 6695 1132 Size: 97 3921 1545 2229 Fragments arranged by size: 3921 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2349 3665 1348 Size: 1001 1316 5475 Fragments arranged by size: 5475 1316 1001 PciI A'CATG_T Cuts at: 6691 6691 Size: 7792 PflMI CCAn_nnn'nTGG Cuts at: 1108 2083 1108 Size: 975 6817 PfoI T'CCnGG_A Cuts at: 2445 3231 3716 3732 4416 4584 2445 Size: 786 485 16 684 168 5653 Fragments arranged by size: 5653 786 684 485 168 16 PmeI GTTT'AAAC Cuts at: 5096 5096 Size: 7792 PmlI CAC'GTG Cuts at: 2182 2365 2182 Size: 183 7609 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2351 2383 5861 5893 7374 7406 2351 Size: 32 3478 32 1481 32 2737 Fragments arranged by size: 3478 2737 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2449 3034 3844 3931 3943 4288 2449 Size: 585 810 87 12 345 5953 Fragments arranged by size: 5953 810 585 345 87 12 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7792 PsiI TTA'TAA Cuts at: 5362 5362 Size: 7792 PspXI vC'TCGA_Gb Cuts at: 5018 5018 Size: 7792 PstI C_TGCA'G Cuts at: 830 2895 3399 3825 3843 4242 4362 4503 4774 Size: 2065 504 426 18 399 120 141 271 Cuts at: 4774 5144 830 Size: 370 3478 Fragments arranged by size: 3478 2065 504 426 399 370 271 141 120 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7792 PvuII CAG'CTG Cuts at: 3226 4145 4243 4345 4516 4944 3226 Size: 919 98 102 171 428 6074 Fragments arranged by size: 6074 919 428 171 102 98 SalI G'TCGA_C Cuts at: 1936 5134 1936 Size: 3198 4594 SapI GCTCTTCn'nnn_ Cuts at: 3240 4901 3240 Size: 1661 6131 SbfI CC_TGCA'GG Cuts at: 3399 3825 3843 5144 3399 Size: 426 18 1301 6047 Fragments arranged by size: 6047 1301 426 18 ScaI AGT'ACT Cuts at: 6040 7752 6040 Size: 1712 6080 SexAI A'CCwGG_T Cuts at: 2715 2715 Size: 7792 SfcI C'TryA_G Cuts at: 826 1045 2891 3395 3821 3839 4238 4358 4499 Size: 219 1846 504 426 18 399 120 141 Cuts at: 4499 4624 4770 4810 5140 6275 6956 7147 826 Size: 125 146 40 330 1135 681 191 1471 Fragments arranged by size: 1846 1471 1135 681 504 426 399 330 219 191 146 141 125 120 40 18 SfiI GGCCn_nnn'nGGCC Cuts at: 4106 4106 Size: 7792 SfoI GGC'GCC Cuts at: 2134 2173 2134 Size: 39 7753 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7792 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7792 SmaI CCC'GGG Cuts at: 1872 2130 5119 1872 Size: 258 2989 4545 Fragments arranged by size: 4545 2989 258 SmlI C'TyrA_G Cuts at: 820 1017 1943 2774 3083 5018 5896 6797 7059 Size: 197 926 831 309 1935 878 901 262 Cuts at: 7059 820 Size: 1553 Fragments arranged by size: 1935 1553 926 901 878 831 309 262 197 SnaBI TAC'GTA Cuts at: 493 5583 493 Size: 5090 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7792 SphI G_CATG'C Cuts at: 1132 5150 1132 Size: 4018 3774 SspI AAT'ATT Cuts at: 5 52 5716 5 Size: 47 5664 2081 Fragments arranged by size: 5664 2081 47 StuI AGG'CCT Cuts at: 1517 3837 4021 4938 1517 Size: 2320 184 917 4371 Fragments arranged by size: 4371 2320 917 184 StyI C'CwwG_G Cuts at: 513 1065 2004 2455 3039 3498 3579 3927 5043 Size: 552 939 451 584 459 81 348 1116 Cuts at: 5043 5231 513 Size: 188 3074 Fragments arranged by size: 3074 1116 939 584 552 459 451 348 188 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2511 2584 4253 4378 4990 4994 5819 Size: 1255 931 73 1669 125 612 4 825 Cuts at: 5819 5836 5989 6174 6619 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1669 1498 1255 931 825 612 445 185 153 125 73 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6038 7750 96 Size: 274 80 33 51 1443 4061 1712 138 Fragments arranged by size: 4061 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4822 4854 7752 7784 4822 Size: 32 2898 32 4830 Fragments arranged by size: 4830 2898 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2189 3536 1316 Size: 873 1347 5572 Fragments arranged by size: 5572 1347 873 XbaI T'CTAG_A Cuts at: 5128 5128 Size: 7792 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2476 2923 2476 Size: 447 7345 XhoI C'TCGA_G Cuts at: 1943 5018 1943 Size: 3075 4717 XmaI C'CCGG_G Cuts at: 1870 2128 5117 1870 Size: 258 2989 4545 Fragments arranged by size: 4545 2989 258 XmnI GAAnn'nnTTC Cuts at: 2297 5921 2297 Size: 3624 4168 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3343 3922 5600 276 Size: 53 83 186 936 1809 579 1678 2468 Fragments arranged by size: 2468 1809 1678 936 579 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI NsiI PacI PspOMI PsrI RsrII SacI SacII SanDI SrfI SwaI