(Circular) (Six-base) MAPSORT of: pFN21AA1401.seq Check: 2589 from: 1 to: 7117 pFN21AA1401 7117 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4927 278 Size: 53 83 186 936 3391 2468 Fragments arranged by size: 3391 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2157 3055 4460 1937 Size: 220 898 1405 4594 Fragments arranged by size: 4594 1405 898 220 Acc65I G'GTAC_C Cuts at: 4241 4438 4241 Size: 197 6920 AclI AA'CG_TT Cuts at: 1563 5244 5617 1563 Size: 3681 373 3063 Fragments arranged by size: 3681 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2615 3706 3714 5180 6564 6832 1632 Size: 983 1091 8 1466 1384 268 1917 Fragments arranged by size: 1917 1466 1384 1091 983 268 8 AfeI AGC'GCT Cuts at: 2645 2645 Size: 7117 AflII C'TTAA_G Cuts at: 820 1017 3521 820 Size: 197 2504 4416 Fragments arranged by size: 4416 2504 197 AflIII A'CryG_T Cuts at: 1203 4149 6016 6993 1203 Size: 2946 1867 977 1327 Fragments arranged by size: 2946 1867 1327 977 AgeI A'CCGG_T Cuts at: 2510 5948 2510 Size: 3438 3679 AhdI GACnn_n'nnGTC Cuts at: 5846 5846 Size: 7117 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7117 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4043 1184 Size: 2859 4258 AlwNI CAG_nnn'CTG Cuts at: 2260 2851 3050 4860 6432 2260 Size: 591 199 1810 1572 2945 Fragments arranged by size: 2945 1810 1572 591 199 ApaLI G'TGCA_C Cuts at: 5174 6330 5174 Size: 1156 5961 ApoI r'AATT_y Cuts at: 1493 2283 2823 3495 4119 4426 4652 4804 6913 Size: 790 540 672 624 307 226 152 2109 Cuts at: 6913 1493 Size: 1697 Fragments arranged by size: 2109 1697 790 672 624 540 307 226 152 AseI AT'TA_AT Cuts at: 160 1032 5671 160 Size: 872 4639 1606 Fragments arranged by size: 4639 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7117 AvaI C'yCGr_G Cuts at: 1870 1943 4442 1870 Size: 73 2499 4545 Fragments arranged by size: 4545 2499 73 BamHI G'GATC_C Cuts at: 3547 4447 4814 3547 Size: 900 367 5850 Fragments arranged by size: 5850 900 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2266 2813 4241 4438 5893 Size: 325 217 627 479 547 1428 197 1455 Cuts at: 5893 618 Size: 1842 Fragments arranged by size: 1842 1455 1428 627 547 479 325 217 197 BanII G_rGCy'C Cuts at: 1391 3846 4436 1391 Size: 2455 590 4072 Fragments arranged by size: 4072 2455 590 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4315 928 Size: 971 2416 3730 Fragments arranged by size: 3730 2416 971 BbvCI CC'TCA_GC Cuts at: 2413 3405 3660 2413 Size: 992 255 5870 Fragments arranged by size: 5870 992 255 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2054 2088 5308 5342 1903 Size: 34 117 34 3220 34 3678 Fragments arranged by size: 3678 3220 117 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5009 6219 5009 Size: 1210 5907 BclI T'GATC_A Cuts at: 1548 1548 Size: 7117 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3956 5728 Size: 107 122 71 631 605 337 1947 1772 Cuts at: 5728 136 Size: 1525 Fragments arranged by size: 1947 1772 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 7112 7112 Size: 7117 BlpI GC'TnA_GC Cuts at: 3131 4534 3131 Size: 1403 5714 Bme1580I G_kGCm'C Cuts at: 1790 2271 2384 2816 5178 6334 1790 Size: 481 113 432 2362 1156 2573 Fragments arranged by size: 2573 2362 1156 481 432 113 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7117 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4848 5806 442 Size: 416 53 83 399 24 3431 958 1753 Fragments arranged by size: 3431 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7117 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4234 5777 1239 Size: 2995 1543 2579 Fragments arranged by size: 2995 2579 1543 Bpu10I CC'TnA_GC Cuts at: 2123 2413 2541 2702 3405 3660 2123 Size: 290 128 161 703 255 5580 Fragments arranged by size: 5580 703 290 255 161 128 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2015 3552 4272 4386 5242 6107 6405 2015 Size: 1537 720 114 856 865 298 2727 Fragments arranged by size: 2727 1537 865 856 720 298 114 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2916 5780 882 Size: 617 1417 2864 2219 Fragments arranged by size: 2864 2219 1417 617 BsaAI yAC'GTr Cuts at: 493 1204 4908 493 Size: 711 3704 2702 Fragments arranged by size: 3704 2702 711 BsaBI GATnn'nnATC Cuts at: 7111 7111 Size: 7117 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4924 5306 5941 275 Size: 53 83 186 936 3391 382 635 1451 Fragments arranged by size: 3391 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2510 3531 5550 5948 6222 6369 6780 2510 Size: 1021 2019 398 274 147 411 2847 Fragments arranged by size: 2847 2019 1021 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3648 3678 3970 4000 1178 Size: 30 165 30 2245 30 292 30 4295 Fragments arranged by size: 4295 2245 292 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2238 2654 3137 3140 3311 1186 Size: 1052 416 483 3 171 4992 Fragments arranged by size: 4992 1052 483 416 171 3 BseYI C'CCAG_C Cuts at: 2227 2499 6320 6816 2227 Size: 272 3821 496 2528 Fragments arranged by size: 3821 2528 496 272 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2067 2258 2417 1163 Size: 573 331 191 159 5863 Fragments arranged by size: 5863 573 331 191 159 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4567 5328 6356 1540 Size: 275 186 2566 761 1028 2301 Fragments arranged by size: 2566 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3846 5178 5263 6334 3846 Size: 1332 85 1071 4629 Fragments arranged by size: 4629 1332 1071 85 BsmI GAATG_Cn' Cuts at: 4032 4628 4721 4032 Size: 596 93 6428 Fragments arranged by size: 6428 596 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3709 3709 Size: 7117 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2271 2384 2816 3846 4436 5178 5263 Size: 399 481 113 432 1030 590 742 85 Cuts at: 5263 6334 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1030 742 590 481 432 399 113 85 BspEI T'CCGG_A Cuts at: 3531 6780 3531 Size: 3249 3868 BspHI T'CATG_A Cuts at: 3763 5006 6736 3763 Size: 1243 1730 4144 Fragments arranged by size: 4144 1730 1243 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4472 844 Size: 3628 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2029 5004 1394 Size: 429 206 2975 3507 Fragments arranged by size: 3507 2975 429 206 BsrDI GCAATG_nn' Cuts at: 65 1248 2985 4027 4038 5612 5786 65 Size: 1183 1737 1042 11 1574 174 1396 Fragments arranged by size: 1737 1574 1396 1183 1042 174 11 BsrFI r'CCGG_y Cuts at: 1700 1810 2510 5761 5948 1700 Size: 110 700 3251 187 2869 Fragments arranged by size: 3251 2869 700 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7117 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7117 BssSI C'ACGA_G Cuts at: 5177 6189 5177 Size: 1012 6105 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7117 BstBI TT'CG_AA Cuts at: 5921 5921 Size: 7117 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7117 BstXI CCAn_nnnn'nTGG Cuts at: 3242 4368 3242 Size: 1126 5991 BstYI r'GATC_y Cuts at: 1972 2078 2428 3088 3547 4066 4447 4814 5199 Size: 106 350 660 459 519 381 367 385 Cuts at: 5199 5216 6668 6754 6766 7112 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 660 519 459 385 381 367 350 346 106 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3552 3552 Size: 7117 BtgI C'CryG_G Cuts at: 513 1065 2004 3542 4246 513 Size: 552 939 1538 704 3384 Fragments arranged by size: 3384 1538 939 704 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2153 487 Size: 683 56 927 5451 Fragments arranged by size: 5451 927 683 56 BtsI GCAGTG_nn' Cuts at: 2640 3900 4633 5427 5447 2640 Size: 1260 733 794 20 4310 Fragments arranged by size: 4310 1260 794 733 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7117 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7082 DraI TTT'AAA Cuts at: 2937 3628 4421 2937 Size: 691 793 5633 Fragments arranged by size: 5633 793 691 DraIII CAC_nnn'GTG Cuts at: 2440 2440 Size: 7117 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4079 6124 809 Size: 507 2763 2045 1802 Fragments arranged by size: 2763 2045 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4249 4564 5453 Size: 54 1425 50 275 18 2419 315 889 Cuts at: 5453 7082 8 Size: 1629 43 Fragments arranged by size: 2419 1629 1425 889 315 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4564 1537 Size: 275 2752 4090 Fragments arranged by size: 4090 2752 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2213 2790 3321 3441 3834 5047 1353 Size: 860 577 531 120 393 1213 3423 Fragments arranged by size: 3423 1213 860 577 531 393 120 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2073 5704 6078 6224 387 Size: 1064 138 484 3631 374 146 1280 Fragments arranged by size: 3631 1280 1064 484 374 146 138 EcoICRI GAG'CTC Cuts at: 3844 3844 Size: 7117 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2615 3706 3714 4234 5180 5777 6564 Size: 393 983 1091 8 520 946 597 787 Cuts at: 6564 6832 1239 Size: 268 1524 Fragments arranged by size: 1524 1091 983 946 787 597 520 393 268 8 EcoNI CCTnn'n_nnAGG Cuts at: 2294 2416 3038 2294 Size: 122 622 6373 Fragments arranged by size: 6373 622 122 EcoO109I rG'GnC_Cy Cuts at: 2545 2965 3181 2545 Size: 420 216 6481 Fragments arranged by size: 6481 420 216 EcoRI G'AATT_C Cuts at: 3495 4426 4804 3495 Size: 931 378 5808 Fragments arranged by size: 5808 931 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3041 3166 3041 Size: 125 6992 FspI TGC'GCA Cuts at: 5623 5623 Size: 7117 HaeII r_GCGC'y Cuts at: 1678 2647 3871 6264 1678 Size: 969 1224 2393 2531 Fragments arranged by size: 2531 2393 1224 969 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3287 3319 4093 4125 4209 Size: 32 135 32 1715 32 774 32 84 Cuts at: 4209 4241 4327 4359 5758 5790 5832 5864 1373 Size: 32 86 32 1399 32 42 32 2626 Fragments arranged by size: 2626 1715 1399 774 135 86 84 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2158 4461 4707 669 Size: 988 281 220 2303 246 3079 Fragments arranged by size: 3079 2303 988 281 246 220 HindIII A'AGCT_T Cuts at: 748 748 Size: 7117 HpaI GTT'AAC Cuts at: 4707 4707 Size: 7117 KpnI G_GTAC'C Cuts at: 4245 4442 4245 Size: 197 6920 MfeI C'AATT_G Cuts at: 4716 4716 Size: 7117 MluI A'CGCG_T Cuts at: 6993 6993 Size: 7117 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2576 2860 3399 6231 6415 1305 Size: 1271 284 539 2832 184 2007 Fragments arranged by size: 2832 2007 1271 539 284 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4251 10 Size: 54 1425 343 2419 2876 Fragments arranged by size: 2876 2419 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2343 2978 3541 5075 5434 5593 518 Size: 648 1177 635 563 1534 359 159 2042 Fragments arranged by size: 2042 1534 1177 648 635 563 359 159 MspA1I CmG'CkG Cuts at: 1606 2089 2227 2308 3908 4533 5210 6358 6603 Size: 483 138 81 1600 625 677 1148 245 Cuts at: 6603 1606 Size: 2120 Fragments arranged by size: 2120 1600 1148 677 625 483 245 138 81 NcoI C'CATG_G Cuts at: 513 1065 2004 3542 4246 513 Size: 552 939 1538 704 3384 Fragments arranged by size: 3384 1538 939 704 552 NdeI CA'TA_TG Cuts at: 387 4348 387 Size: 3961 3156 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7117 NotI GC'GGCC_GC Cuts at: 4564 4564 Size: 7117 NspI r_CATG'y Cuts at: 1132 1229 4153 4475 6020 1132 Size: 97 2924 322 1545 2229 Fragments arranged by size: 2924 2229 1545 322 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7117 PciI A'CATG_T Cuts at: 4149 6016 4149 Size: 1867 5250 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7117 PmeI GTTT'AAAC Cuts at: 4421 4421 Size: 7117 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5186 5218 6699 6731 5186 Size: 32 1481 32 5572 Fragments arranged by size: 5572 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2965 3181 2965 Size: 216 6901 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7117 PsiI TTA'TAA Cuts at: 4687 4687 Size: 7117 PstI C_TGCA'G Cuts at: 830 3907 4469 830 Size: 3077 562 3478 Fragments arranged by size: 3478 3077 562 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7117 PvuII CAG'CTG Cuts at: 2227 2308 2227 Size: 81 7036 SacI G_AGCT'C Cuts at: 3846 3846 Size: 7117 SalI G'TCGA_C Cuts at: 1936 2156 4459 1936 Size: 220 2303 4594 Fragments arranged by size: 4594 2303 220 SapI GCTCTTCn'nnn_ Cuts at: 3834 3834 Size: 7117 SbfI CC_TGCA'GG Cuts at: 4469 4469 Size: 7117 ScaI AGT'ACT Cuts at: 4299 5365 7077 4299 Size: 1066 1712 4339 Fragments arranged by size: 4339 1712 1066 SfcI C'TryA_G Cuts at: 826 1045 3003 3903 4044 4465 5600 6281 6472 Size: 219 1958 900 141 421 1135 681 191 Cuts at: 6472 826 Size: 1471 Fragments arranged by size: 1958 1471 1135 900 681 421 219 191 141 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7117 SgrAI Cr'CCGG_yG Cuts at: 1700 2510 1700 Size: 810 6307 SmaI CCC'GGG Cuts at: 1872 4444 1872 Size: 2572 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2030 3521 3567 4287 4401 5221 Size: 197 926 87 1491 46 720 114 820 Cuts at: 5221 6122 6384 820 Size: 901 262 1553 Fragments arranged by size: 1553 1491 926 901 820 720 262 197 114 87 46 SnaBI TAC'GTA Cuts at: 493 4908 493 Size: 4415 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7117 SphI G_CATG'C Cuts at: 1132 4475 1132 Size: 3343 3774 SspI AAT'ATT Cuts at: 5 52 3585 5041 5 Size: 47 3533 1456 2081 Fragments arranged by size: 3533 2081 1456 47 StuI AGG'CCT Cuts at: 1517 3519 1517 Size: 2002 5115 StyI C'CwwG_G Cuts at: 513 1065 2004 3542 3834 4246 4556 513 Size: 552 939 1538 292 412 310 3074 Fragments arranged by size: 3074 1538 939 552 412 310 292 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2219 2375 5144 5161 5314 5499 5944 Size: 1255 639 156 2769 17 153 185 445 Cuts at: 5944 325 Size: 1498 Fragments arranged by size: 2769 1498 1255 639 445 185 156 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2335 4297 5363 Size: 274 80 33 51 1443 358 1962 1066 Cuts at: 5363 7075 96 Size: 1712 138 Fragments arranged by size: 1962 1712 1443 1066 358 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2553 2585 3970 4002 4160 4192 7077 7109 2553 Size: 32 1385 32 158 32 2885 32 2561 Fragments arranged by size: 2885 2561 1385 158 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7117 XbaI T'CTAG_A Cuts at: 4453 4453 Size: 7117 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2496 2579 2496 Size: 83 7034 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7117 XmaI C'CCGG_G Cuts at: 1870 4442 1870 Size: 2572 4545 XmnI GAAnn'nnTTC Cuts at: 3561 5246 3561 Size: 1685 5432 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4925 276 Size: 53 83 186 936 3391 2468 Fragments arranged by size: 3391 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI