(Circular) (Six-base) MAPSORT of: pFN21AA1381.seq Check: 5196 from: 1 to: 7645 pFN21AA1381 7645 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2530 2982 2530 Size: 452 7193 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5455 278 Size: 53 83 186 936 3919 2468 Fragments arranged by size: 3919 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4988 1937 Size: 3051 4594 Acc65I G'GTAC_C Cuts at: 2169 4966 2169 Size: 2797 4848 AclI AA'CG_TT Cuts at: 1563 5772 6145 1563 Size: 4209 373 3063 Fragments arranged by size: 4209 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2054 2822 3599 3908 5708 7092 7360 1632 Size: 422 768 777 309 1800 1384 268 1917 Fragments arranged by size: 1917 1800 1384 777 768 422 309 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7448 AflIII A'CryG_T Cuts at: 1203 3072 3414 4893 6544 7521 1203 Size: 1869 342 1479 1651 977 1327 Fragments arranged by size: 1869 1651 1479 1327 977 342 AgeI A'CCGG_T Cuts at: 6476 6476 Size: 7645 AhdI GACnn_n'nnGTC Cuts at: 4874 4894 6374 4874 Size: 20 1480 6145 Fragments arranged by size: 6145 1480 20 AleI CACnn'nnGTG Cuts at: 1166 2745 1166 Size: 1579 6066 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2609 2829 3822 1184 Size: 1425 220 993 5007 Fragments arranged by size: 5007 1425 993 220 AlwNI CAG_nnn'CTG Cuts at: 3265 3289 3855 3865 4184 4853 5388 6960 3265 Size: 24 566 10 319 669 535 1572 3950 Fragments arranged by size: 3950 1572 669 566 535 319 24 10 ApaI G_GGCC'C Cuts at: 2314 2314 Size: 7645 ApaLI G'TGCA_C Cuts at: 5702 6858 5702 Size: 1156 6489 ApoI r'AATT_y Cuts at: 1493 2382 4164 4954 5180 5332 7441 1493 Size: 889 1782 790 226 152 2109 1697 Fragments arranged by size: 2109 1782 1697 889 790 226 152 AseI AT'TA_AT Cuts at: 160 1032 4272 6199 160 Size: 872 3240 1927 1606 Fragments arranged by size: 3240 1927 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7645 AvaI C'yCGr_G Cuts at: 1870 1943 4970 1870 Size: 73 3027 4545 Fragments arranged by size: 4545 3027 73 BamHI G'GATC_C Cuts at: 3066 4975 5342 3066 Size: 1909 367 5369 Fragments arranged by size: 5369 1909 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2169 4966 6421 618 Size: 325 217 627 382 2797 1455 1842 Fragments arranged by size: 2797 1842 1455 627 382 325 217 BanII G_rGCy'C Cuts at: 1391 2314 2405 3336 3920 3949 4964 1391 Size: 923 91 931 584 29 1015 4072 Fragments arranged by size: 4072 1015 931 923 584 91 29 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3090 4893 928 Size: 971 1191 1803 3680 Fragments arranged by size: 3680 1803 1191 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5836 5870 1903 Size: 34 3899 34 3678 Fragments arranged by size: 3899 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5537 6747 5537 Size: 1210 6435 BclI T'GATC_A Cuts at: 1548 1548 Size: 7645 BfrBI ATG'CAT Cuts at: 3735 3735 Size: 7645 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2015 2246 2283 Size: 107 122 71 631 605 343 231 37 Cuts at: 2283 2288 2708 6256 136 Size: 5 420 3548 1525 Fragments arranged by size: 3548 1525 631 605 420 343 231 122 107 71 37 5 BglII A'GATC_T Cuts at: 2394 7640 2394 Size: 5246 2399 BlpI GC'TnA_GC Cuts at: 5062 5062 Size: 7645 Bme1580I G_kGCm'C Cuts at: 1790 2314 2927 3881 5706 6862 1790 Size: 524 613 954 1825 1156 2573 Fragments arranged by size: 2573 1825 1156 954 613 524 BmgBI CAC'GTC Cuts at: 1318 4896 1318 Size: 3578 4067 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4093 5376 6334 Size: 416 53 83 399 24 2676 1283 958 Cuts at: 6334 442 Size: 1753 Fragments arranged by size: 2676 1753 1283 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7645 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2688 4381 2688 Size: 1693 5952 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2123 2419 2459 2909 3245 3301 4013 4078 Size: 884 296 40 450 336 56 712 65 Cuts at: 4078 4495 6305 1239 Size: 417 1810 2579 Fragments arranged by size: 2579 1810 884 712 450 417 336 296 65 56 40 Bpu10I CC'TnA_GC Cuts at: 4806 4806 Size: 7645 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5770 6635 6933 5770 Size: 865 298 6482 Fragments arranged by size: 6482 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2885 3143 6308 882 Size: 617 1386 258 3165 2219 Fragments arranged by size: 3165 2219 1386 617 258 BsaAI yAC'GTr Cuts at: 493 1204 3121 5436 493 Size: 711 1917 2315 2702 Fragments arranged by size: 2702 2315 1917 711 BsaBI GATnn'nnATC Cuts at: 7639 7639 Size: 7645 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2238 3156 5452 5834 Size: 53 83 186 936 705 918 2296 382 Cuts at: 5834 6469 275 Size: 635 1451 Fragments arranged by size: 2296 1451 936 918 705 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2386 2562 6078 6476 6750 6897 7308 2386 Size: 176 3516 398 274 147 411 2723 Fragments arranged by size: 3516 2723 411 398 274 176 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3299 3329 4166 4196 1178 Size: 30 165 30 1896 30 837 30 4627 Fragments arranged by size: 4627 1896 837 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2102 2156 2661 4016 4187 1186 Size: 916 54 505 1355 171 4644 Fragments arranged by size: 4644 1355 916 505 171 54 BseYI C'CCAG_C Cuts at: 2313 3198 4149 6848 7344 2313 Size: 885 951 2699 496 2614 Fragments arranged by size: 2699 2614 951 885 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3722 3812 4086 4203 4631 4884 1163 Size: 573 1986 90 274 117 428 253 3924 Fragments arranged by size: 3924 1986 573 428 274 253 117 90 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2193 2292 5095 5856 6884 1540 Size: 275 186 192 99 2803 761 1028 2301 Fragments arranged by size: 2803 2301 1028 761 275 192 186 99 BsiHKAI G_wGCw'C Cuts at: 2133 2405 2645 3336 3920 5706 5791 6862 2133 Size: 272 240 691 584 1786 85 1071 2916 Fragments arranged by size: 2916 1786 1071 691 584 272 240 85 BsmI GAATG_Cn' Cuts at: 5156 5249 5156 Size: 93 7552 BsmBI CGTCTCn'nnnn_ Cuts at: 2066 2066 Size: 7645 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2133 2314 2405 2645 2927 3336 3881 Size: 399 343 181 91 240 282 409 545 Cuts at: 3881 3920 3949 4964 5706 5791 6862 1391 Size: 39 29 1015 742 85 1071 2174 Fragments arranged by size: 2174 1071 1015 742 545 409 399 343 282 240 181 91 85 39 29 BspEI T'CCGG_A Cuts at: 2386 7308 2386 Size: 4922 2723 BspHI T'CATG_A Cuts at: 5534 7264 5534 Size: 1730 5915 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2454 2530 2982 3117 3459 5000 844 Size: 1610 76 452 135 342 1541 3489 Fragments arranged by size: 3489 1610 1541 452 342 135 76 BsrBI CCG'CTC Cuts at: 1394 1823 2084 3235 5532 1394 Size: 429 261 1151 2297 3507 Fragments arranged by size: 3507 2297 1151 429 261 BsrDI GCAATG_nn' Cuts at: 65 1248 6140 6314 65 Size: 1183 4892 174 1396 Fragments arranged by size: 4892 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2282 6289 6476 1700 Size: 110 472 4007 187 2869 Fragments arranged by size: 4007 2869 472 187 110 BsrGI T'GTAC_A Cuts at: 96 3544 4237 96 Size: 3448 693 3504 Fragments arranged by size: 3504 3448 693 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7645 BssSI C'ACGA_G Cuts at: 5705 6717 5705 Size: 1012 6633 BstAPI GCAn_nnn'nTGC Cuts at: 794 3709 794 Size: 2915 4730 BstBI TT'CG_AA Cuts at: 6449 6449 Size: 7645 BstEII G'GTnAC_C Cuts at: 1184 4838 1184 Size: 3654 3991 BstXI CCAn_nnnn'nTGG Cuts at: 2750 2750 Size: 7645 BstYI r'GATC_y Cuts at: 1972 2044 2091 2394 2851 3066 4206 4975 5342 Size: 72 47 303 457 215 1140 769 367 Cuts at: 5342 5727 5744 7196 7282 7294 7640 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1140 769 457 385 367 346 303 215 86 72 47 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2013 2095 2223 2306 2743 3726 Size: 552 939 9 82 128 83 437 983 Cuts at: 3726 513 Size: 4432 Fragments arranged by size: 4432 983 939 552 437 128 83 82 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4586 487 Size: 683 56 3360 3546 Fragments arranged by size: 3546 3360 683 56 BtsI GCAGTG_nn' Cuts at: 3629 3899 4069 4198 5161 5955 5975 3629 Size: 270 170 129 963 794 20 5299 Fragments arranged by size: 5299 963 794 270 170 129 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7645 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7610 DraI TTT'AAA Cuts at: 4949 4949 Size: 7645 DraIII CAC_nnn'GTG Cuts at: 3748 3748 Size: 7645 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6652 809 Size: 507 5336 1802 Fragments arranged by size: 5336 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2190 2203 Size: 54 1425 50 275 18 177 183 13 Cuts at: 2203 2289 2300 2628 2700 2790 3846 4134 4492 Size: 86 11 328 72 90 1056 288 358 Cuts at: 4492 5092 5981 7610 8 Size: 600 889 1629 43 Fragments arranged by size: 1629 1425 1056 889 600 358 328 288 275 183 177 90 86 72 54 50 43 18 13 11 EagI C'GGCC_G Cuts at: 1537 1812 2190 2289 5092 1537 Size: 275 378 99 2803 4090 Fragments arranged by size: 4090 2803 378 275 99 EarI CTCTTCn'nnn_ Cuts at: 1353 2655 2882 2937 3119 3428 3777 4481 5575 Size: 1302 227 55 182 309 349 704 1094 Cuts at: 5575 1353 Size: 3423 Fragments arranged by size: 3423 1302 1094 704 349 309 227 182 55 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2264 6232 6606 6752 387 Size: 1064 138 675 3968 374 146 1280 Fragments arranged by size: 3968 1280 1064 675 374 146 138 EcoICRI GAG'CTC Cuts at: 2403 3334 3918 2403 Size: 931 584 6130 Fragments arranged by size: 6130 931 584 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2123 2419 2459 2822 2909 3245 Size: 393 422 69 296 40 363 87 336 Cuts at: 3245 3301 3599 3908 4013 4078 4495 5708 6305 Size: 56 298 309 105 65 417 1213 597 Cuts at: 6305 7092 7360 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1213 787 597 422 417 393 363 336 309 298 296 268 105 87 69 65 56 40 EcoNI CCTnn'n_nnAGG Cuts at: 2183 2276 2183 Size: 93 7552 EcoO109I rG'GnC_Cy Cuts at: 2635 2635 Size: 7645 EcoRI G'AATT_C Cuts at: 4954 5332 4954 Size: 378 7267 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2392 3253 3352 2392 Size: 861 99 6685 Fragments arranged by size: 6685 861 99 FspI TGC'GCA Cuts at: 6151 6151 Size: 7645 HaeII r_GCGC'y Cuts at: 1678 2110 4424 6792 1678 Size: 432 2314 2368 2531 Fragments arranged by size: 2531 2368 2314 432 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3299 3331 3611 3643 4109 Size: 32 135 32 1727 32 280 32 466 Cuts at: 4109 4141 4166 4198 4750 4782 4860 4880 4892 Size: 32 25 32 552 32 78 20 12 Cuts at: 4892 4912 6286 6318 6360 6392 1373 Size: 20 1374 32 42 32 2626 Fragments arranged by size: 2626 1727 1374 552 466 280 135 78 42 32 32 32 32 32 32 32 32 32 25 20 20 12 HincII GTy'rAC Cuts at: 669 1657 1938 2568 4989 5235 669 Size: 988 281 630 2421 246 3079 Fragments arranged by size: 3079 2421 988 630 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7645 HpaI GTT'AAC Cuts at: 5235 5235 Size: 7645 KpnI G_GTAC'C Cuts at: 2173 4970 2173 Size: 2797 4848 MfeI C'AATT_G Cuts at: 5244 5244 Size: 7645 MluI A'CGCG_T Cuts at: 7521 7521 Size: 7645 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4234 4261 6759 6943 1305 Size: 2929 27 2498 184 2007 Fragments arranged by size: 2929 2498 2007 184 27 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2702 2792 3848 4136 Size: 54 1425 343 177 693 90 1056 288 Cuts at: 4136 10 Size: 3519 Fragments arranged by size: 3519 1425 1056 693 343 288 177 90 54 MslI CAynn'nnrTG Cuts at: 518 1166 2745 3119 5603 5962 6121 518 Size: 648 1579 374 2484 359 159 2042 Fragments arranged by size: 2484 2042 1579 648 374 359 159 MspA1I CmG'CkG Cuts at: 1606 2015 2097 2217 2308 2479 2961 3198 4149 Size: 409 82 120 91 171 482 237 951 Cuts at: 4149 4429 4835 5061 5738 6886 7131 1606 Size: 280 406 226 677 1148 245 2120 Fragments arranged by size: 2120 1148 951 677 482 409 406 280 245 237 226 171 120 91 82 NaeI GCC'GGC Cuts at: 2284 2284 Size: 7645 NcoI C'CATG_G Cuts at: 513 1065 2004 2743 513 Size: 552 939 739 5415 Fragments arranged by size: 5415 939 739 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7645 NgoMIV G'CCGG_C Cuts at: 2282 2282 Size: 7645 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7645 NotI GC'GGCC_GC Cuts at: 5092 5092 Size: 7645 NsiI A_TGCA'T Cuts at: 3737 3737 Size: 7645 NspI r_CATG'y Cuts at: 1132 1229 3076 3418 3735 5003 6548 1132 Size: 97 1847 342 317 1268 1545 2229 Fragments arranged by size: 2229 1847 1545 1268 342 317 97 PasI CC'CwG_GG Cuts at: 1348 3865 1348 Size: 2517 5128 PciI A'CATG_T Cuts at: 3072 3414 6544 3072 Size: 342 3130 4173 Fragments arranged by size: 4173 3130 342 PflMI CCAn_nnn'nTGG Cuts at: 1108 2626 2744 3865 4149 1108 Size: 1518 118 1121 284 4604 Fragments arranged by size: 4604 1518 1121 284 118 PfoI T'CCnGG_A Cuts at: 3150 3150 Size: 7645 PmeI GTTT'AAAC Cuts at: 4949 4949 Size: 7645 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5714 5746 7227 7259 5714 Size: 32 1481 32 6100 Fragments arranged by size: 6100 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7645 PsiI TTA'TAA Cuts at: 5215 5215 Size: 7645 PspOMI G'GGCC_C Cuts at: 2310 2310 Size: 7645 PstI C_TGCA'G Cuts at: 830 2940 2960 3060 4428 4997 830 Size: 2110 20 100 1368 569 3478 Fragments arranged by size: 3478 2110 1368 569 100 20 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7645 PvuII CAG'CTG Cuts at: 2479 2961 3198 4149 4429 4835 2479 Size: 482 237 951 280 406 5289 Fragments arranged by size: 5289 951 482 406 280 237 SacI G_AGCT'C Cuts at: 2405 3336 3920 2405 Size: 931 584 6130 Fragments arranged by size: 6130 931 584 SacII CC_GC'GG Cuts at: 2016 2098 2309 2016 Size: 82 211 7352 Fragments arranged by size: 7352 211 82 SalI G'TCGA_C Cuts at: 1936 4987 1936 Size: 3051 4594 SapI GCTCTTCn'nnn_ Cuts at: 2882 3119 3428 2882 Size: 237 309 7099 Fragments arranged by size: 7099 309 237 SbfI CC_TGCA'GG Cuts at: 2940 4997 2940 Size: 2057 5588 ScaI AGT'ACT Cuts at: 2261 4023 5893 7605 2261 Size: 1762 1870 1712 2301 Fragments arranged by size: 2301 1870 1762 1712 SexAI A'CCwGG_T Cuts at: 4050 4050 Size: 7645 SfcI C'TryA_G Cuts at: 826 1045 2347 2936 2956 3056 3770 4424 4993 Size: 219 1302 589 20 100 714 654 569 Cuts at: 4993 6128 6809 7000 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1302 1135 714 681 654 589 569 219 191 100 20 SfiI GGCCn_nnn'nGGCC Cuts at: 2288 2288 Size: 7645 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7645 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7645 SmaI CCC'GGG Cuts at: 1872 4972 1872 Size: 3100 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5749 6650 6912 820 Size: 197 926 3806 901 262 1553 Fragments arranged by size: 3806 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5436 493 Size: 4943 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7645 SphI G_CATG'C Cuts at: 1132 3735 5003 1132 Size: 2603 1268 3774 Fragments arranged by size: 3774 2603 1268 SspI AAT'ATT Cuts at: 5 52 5569 5 Size: 47 5517 2081 Fragments arranged by size: 5517 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7645 StyI C'CwwG_G Cuts at: 513 1065 2004 2605 2743 3327 3636 4470 5084 Size: 552 939 601 138 584 309 834 614 Cuts at: 5084 513 Size: 3074 Fragments arranged by size: 3074 939 834 614 601 584 552 309 138 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3291 4112 4424 5672 5689 5842 6027 Size: 1255 1711 821 312 1248 17 153 185 Cuts at: 6027 6472 325 Size: 445 1498 Fragments arranged by size: 1711 1498 1255 1248 821 445 312 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2259 3408 3544 Size: 274 80 33 51 1443 282 1149 136 Cuts at: 3544 4021 4237 5891 7603 96 Size: 477 216 1654 1712 138 Fragments arranged by size: 1712 1654 1443 1149 477 282 274 216 138 136 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3146 3178 7605 7637 3146 Size: 32 4427 32 3154 Fragments arranged by size: 4427 3154 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3721 1316 Size: 2405 5240 XbaI T'CTAG_A Cuts at: 4111 4981 4111 Size: 870 6775 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2741 3334 2741 Size: 593 7052 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7645 XmaI C'CCGG_G Cuts at: 1870 4970 1870 Size: 3100 4545 XmnI GAAnn'nnTTC Cuts at: 3984 5774 3984 Size: 1790 5855 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5453 276 Size: 53 83 186 936 3919 2468 Fragments arranged by size: 3919 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspXI PsrI RsrII SanDI SfoI SrfI SwaI