Restriction map for
pFN21AA1343
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AflII AflIII AlwNI Aor51HI ApaI
AscI Asp718I AspI AvaI AviII
AvrII BanI BanII BbeI BbrPI
BbsI BbvCI BclI BfrI BglI
BglII BlnI BpuAI BsaAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CfoI ClaI CpoI DraII DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HhaI HinP1I HindIII HpaI
KasI KpnI KspI MluI MslI
MspA1I MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI TaqI Tth111I TthHB8I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccIII 255 TCCGGA T^CCGGA
AciI 906 CCGC CCGC(-3/-1)
AcyI 995 GGCGTC G[GA]^CG[CT]C
AfaI 60 GTAC GT^AC
AgeI 1407 ACCGGT A^CCGGT
AhdI 1167 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 791 GTGCAC G^TGCAC
ApaLI 791 GTGCAC G^TGCAC
AseI 856 ATTAAT AT^TAAT
AsnI 856 ATTAAT AT^TAAT
Asp700I 972 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1167 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 963 TGGCCA TGG^CCA
BamHI 29 GGATCC G^GATCC
BcgI 1308 GCAGCAGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 110 GTATCC GTATCC(6/5)
BlpI 775 GCTAAGC GC^T[ACGT]AGC
BpmI 1303 CTCCAG CTGGAG(16/14)
Bpu1102I 775 GCTAAGC GC^T[ACGT]AGC
BsaBI 514 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 995 GGCGTC G[GA]^CG[CT]C
BsaI 95 GGTCTC GGTCTC(1/5)
BseAI 255 TCCGGA T^CCGGA
BsgI 1480 CTGCAC GTGCAG(16/14)
BsmI 771 GAATGC GAATGC(1/-1)
BspEI 255 TCCGGA T^CCGGA
BsrDI 138 CATTGC GCAATG(2/0)
BstYI 29 GGATCC [GA]^GATC[CT]
CelII 775 GCTAAGC GC^T[ACGT]AGC
DraI 1341 TTTAAA TTT^AAA
DsaI 1418 CCACGG C^C[GA][CT]GG
Eam1105I 1167 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HgaI 996 GCGTC GACGC(5/10)
Hin1I 995 GGCGTC G[GA]^CG[CT]C
HincII 1430 GTCAAC GT[CT]^[GA]AC
HindII 1430 GTCAAC GT[CT]^[GA]AC
MaeII 759 ACGT A^CGT
MaeIII 461 GTGAC ^GT[ACGT]AC
MamI 514 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 29 GGATCC [GA]^GATC[CT]
MluNI 963 TGGCCA TGG^CCA
MroI 255 TCCGGA T^CCGGA
MscI 963 TGGCCA TGG^CCA
PflMI 493 CCAATGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1407 ACCGGT A^CCGGT
PshBI 856 ATTAAT AT^TAAT
RsaI 60 GTAC GT^AC
SspI 374 AATATT AAT^ATT
Tsp45I 461 GTGAC ^GT[GC]AC
Van91I 493 CCAATGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1050 CCAGGCTTCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 29 GGATCC [GA]^GATC[CT]
XmnI 972 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AocI 652 CCTAAGG CC^T[ACGT]AGG
723 CCTGAGG
AspHI 791 GTGCAC G[AT]GC[AT]^C
1122 GAGCAC
AvaII 869 GGACC G^G[AT]CC
1174 GGTCC
BcnI 1275 CCCGG CC^[GC]GG
1331 CCCGG
BmyI 791 GTGCAC G[AGT]GC[ACT]^C
1122 GAGCAC
BsaWI 255 TCCGGA [AT]^CCGG[AT]
1407 ACCGGT
BsiHKAI 791 GTGCAC G[AT]GC[AT]^C
1122 GAGCAC
BsmAI 1087 GAGAC GTCTC(1/5)
96 GTCTC
BsmFI 222 GTCCC GGGAC(10/14)
701 GTCCC
Bsp1286I 791 GTGCAC G[AGT]GC[ACT]^C
1122 GAGCAC
BsrFI 1286 ACCGGC [GA]^CCGG[CT]
1407 ACCGGT
BsrI 732 ACTGG ACTGG(1/-1)
1170 ACTGG
Bsu36I 652 CCTAAGG CC^T[ACGT]AGG
723 CCTGAGG
Cfr10I 1286 ACCGGC [GA]^CCGG[CT]
1407 ACCGGT
Cfr13I 869 GGACC G^G[ACGT]CC
1174 GGTCC
EaeI 963 TGGCCA [CT]^GGCC[GA]
1333 CGGCCA
Eco81I 652 CCTAAGG CC^T[ACGT]AGG
723 CCTGAGG
EcoRI 826 GAATTC G^AATTC
1454 GAATTC
HaeIII 964 GGCC GG^CC
1334 GGCC
MfeI 143 CAATTG C^AATTG
356 CAATTG
MunI 143 CAATTG C^AATTG
356 CAATTG
MwoI 1216 GCCCCTATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1270 GCTGCCCCGGC
NciI 1275 CCCGG CC^[GC]GG
1331 CCCGG
NspI 188 GCATGT [GA]CATG^[CT]
659 GCATGT
PleI 5 GAGTC GAGTC(4/5)
841 GAGTC
SapI 1119 GAAGAGC GCTCTTC(1/4)
1279 GCTCTTC
Sau96I 869 GGACC G^G[ACGT]CC
1174 GGTCC
SfcI 696 CTGTAG C^T[GA][CT]AG
990 CTACAG
TfiI 330 GATTC G^A[AT]TC
1465 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbvI
BfaI BsaJI BseRI BsiYI BslI
BssKI BstF5I BstNI Cac8I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MboI MboII MnlI
MseI MspI MvaI NdeII NlaIII
NlaIV Sau3AI ScrFI SfaNI Tru9I
TseI Tsp509I TspRI