(Circular) (Six-base) MAPSORT of: pFN21AA1343.seq Check: 305 from: 1 to: 6190 pFN21AA1343 6190 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4000 278 Size: 53 83 186 936 2464 2468 Fragments arranged by size: 2468 2464 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3533 1937 Size: 1596 4594 Acc65I G'GTAC_C Cuts at: 3511 3511 Size: 6190 AclI AA'CG_TT Cuts at: 1563 4317 4690 1563 Size: 2754 373 3063 Fragments arranged by size: 3063 2754 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4253 5637 5905 1632 Size: 2621 1384 268 1917 Fragments arranged by size: 2621 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5993 AflIII A'CryG_T Cuts at: 1203 5089 6066 1203 Size: 3886 977 1327 Fragments arranged by size: 3886 1327 977 AgeI A'CCGG_T Cuts at: 3412 5021 3412 Size: 1609 4581 AhdI GACnn_n'nnGTC Cuts at: 3177 4919 3177 Size: 1742 4448 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6190 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6190 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2553 2585 2553 Size: 32 6158 AlwNI CAG_nnn'CTG Cuts at: 3933 5505 3933 Size: 1572 4618 ApaLI G'TGCA_C Cuts at: 2796 4247 5403 2796 Size: 1451 1156 3583 Fragments arranged by size: 3583 1451 1156 ApoI r'AATT_y Cuts at: 1493 2042 2831 3459 3499 3725 3877 5986 1493 Size: 549 789 628 40 226 152 2109 1697 Fragments arranged by size: 2109 1697 789 628 549 226 152 40 AseI AT'TA_AT Cuts at: 160 1032 2862 4744 160 Size: 872 1830 1882 1606 Fragments arranged by size: 1882 1830 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6190 AvaI C'yCGr_G Cuts at: 1870 1943 3515 1870 Size: 73 1572 4545 Fragments arranged by size: 4545 1572 73 BamHI G'GATC_C Cuts at: 2034 3520 3887 2034 Size: 1486 367 4337 Fragments arranged by size: 4337 1486 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3511 4966 618 Size: 325 217 627 1724 1455 1842 Fragments arranged by size: 1842 1724 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3509 1391 Size: 2118 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5219 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3302 3336 4381 4415 1903 Size: 34 1365 34 1045 34 3678 Fragments arranged by size: 3678 1365 1045 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2126 4082 5292 2126 Size: 1956 1210 3024 Fragments arranged by size: 3024 1956 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6190 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4801 136 Size: 107 122 71 631 605 3129 1525 Fragments arranged by size: 3129 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6185 6185 Size: 6190 BlpI GC'TnA_GC Cuts at: 2781 3607 2781 Size: 826 5364 Bme1580I G_kGCm'C Cuts at: 1790 2800 4251 5407 1790 Size: 1010 1451 1156 2573 Fragments arranged by size: 2573 1451 1156 1010 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6190 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3185 3921 4879 Size: 416 53 83 399 24 1768 736 958 Cuts at: 4879 442 Size: 1753 Fragments arranged by size: 1768 1753 958 736 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6190 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2770 2770 Size: 6190 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3293 4850 1239 Size: 2054 1557 2579 Fragments arranged by size: 2579 2054 1557 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4315 5180 5478 4315 Size: 865 298 5027 Fragments arranged by size: 5027 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2106 4853 882 Size: 617 607 2747 2219 Fragments arranged by size: 2747 2219 617 607 BsaAI yAC'GTr Cuts at: 493 1204 3981 493 Size: 711 2777 2702 Fragments arranged by size: 2777 2702 711 BsaBI GATnn'nnATC Cuts at: 2523 6184 2523 Size: 3661 2529 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3001 3997 4379 5014 Size: 53 83 186 936 1468 996 382 635 Cuts at: 5014 275 Size: 1451 Fragments arranged by size: 1468 1451 996 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2260 3412 4623 5021 5295 5442 5853 2260 Size: 1152 1211 398 274 147 411 2597 Fragments arranged by size: 2597 1211 1152 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5965 Fragments arranged by size: 5965 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2897 3032 3104 3156 3158 3291 1186 Size: 1711 135 72 52 2 133 4085 Fragments arranged by size: 4085 1711 135 133 72 52 2 BseYI C'CCAG_C Cuts at: 5393 5889 5393 Size: 496 5694 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3470 1163 Size: 573 1734 3883 Fragments arranged by size: 3883 1734 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3640 4401 5429 1540 Size: 275 186 1639 761 1028 2301 Fragments arranged by size: 2301 1639 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2800 3131 4251 4336 5407 2800 Size: 331 1120 85 1071 3583 Fragments arranged by size: 3583 1120 1071 331 85 BsmI GAATG_Cn' Cuts at: 2782 3701 3794 2782 Size: 919 93 5178 Fragments arranged by size: 5178 919 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2800 3131 3509 4251 4336 5407 1391 Size: 399 1010 331 378 742 85 1071 2174 Fragments arranged by size: 2174 1071 1010 742 399 378 331 85 BspEI T'CCGG_A Cuts at: 2260 5853 2260 Size: 3593 2597 BspHI T'CATG_A Cuts at: 4079 5809 4079 Size: 1730 4460 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3545 844 Size: 2701 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4077 1394 Size: 429 2254 3507 Fragments arranged by size: 3507 2254 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2142 4685 4859 65 Size: 1183 894 2543 174 1396 Fragments arranged by size: 2543 1396 1183 894 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3291 3412 4834 5021 1700 Size: 110 1481 121 1422 187 2869 Fragments arranged by size: 2869 1481 1422 187 121 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6190 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6190 BssSI C'ACGA_G Cuts at: 4250 5262 4250 Size: 1012 5178 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6190 BstBI TT'CG_AA Cuts at: 4994 4994 Size: 6190 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6190 BstYI r'GATC_y Cuts at: 1972 2034 3520 3887 4272 4289 5741 5827 5839 Size: 62 1486 367 385 17 1452 86 12 Cuts at: 5839 6185 1972 Size: 346 1977 Fragments arranged by size: 1977 1486 1452 385 367 346 86 62 17 12 Bsu36I CC'TnA_GG Cuts at: 2658 2729 2658 Size: 71 6119 BtgI C'CryG_G Cuts at: 513 1065 3423 513 Size: 552 2358 3280 Fragments arranged by size: 3280 2358 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2606 487 Size: 683 56 1380 4071 Fragments arranged by size: 4071 1380 683 56 BtsI GCAGTG_nn' Cuts at: 2904 3266 3482 3706 4500 4520 2904 Size: 362 216 224 794 20 4574 Fragments arranged by size: 4574 794 362 224 216 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6190 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6155 DraI TTT'AAA Cuts at: 3348 3494 3348 Size: 146 6044 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5197 809 Size: 507 3881 1802 Fragments arranged by size: 3881 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2968 3338 3637 Size: 54 1425 50 275 18 1138 370 299 Cuts at: 3637 4526 6155 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1138 889 370 299 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3637 1537 Size: 275 1825 4090 Fragments arranged by size: 4090 1825 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2593 3119 3135 3291 4120 1353 Size: 1240 526 16 156 829 3423 Fragments arranged by size: 3423 1240 829 526 156 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4777 5151 5297 387 Size: 1064 138 3188 374 146 1280 Fragments arranged by size: 3188 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3293 4253 4850 5637 5905 1239 Size: 393 1661 960 597 787 268 1524 Fragments arranged by size: 1661 1524 960 787 597 393 268 EcoRI G'AATT_C Cuts at: 2831 3459 3499 3877 2831 Size: 628 40 378 5144 Fragments arranged by size: 5144 628 378 40 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3001 3001 Size: 6190 FspI TGC'GCA Cuts at: 4696 4696 Size: 6190 HaeII r_GCGC'y Cuts at: 1678 5337 1678 Size: 3659 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3163 3195 3461 3493 4831 Size: 32 135 32 1591 32 266 32 1338 Cuts at: 4831 4863 4905 4937 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1591 1338 266 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3437 3534 3780 669 Size: 988 281 1499 97 246 3079 Fragments arranged by size: 3079 1499 988 281 246 97 HindIII A'AGCT_T Cuts at: 748 748 Size: 6190 HpaI GTT'AAC Cuts at: 3780 3780 Size: 6190 KpnI G_GTAC'C Cuts at: 3515 3515 Size: 6190 MfeI C'AATT_G Cuts at: 2148 2361 3789 2148 Size: 213 1428 4549 Fragments arranged by size: 4549 1428 213 MluI A'CGCG_T Cuts at: 6066 6066 Size: 6190 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1993 2852 3233 3287 5304 5488 1305 Size: 688 859 381 54 2017 184 2007 Fragments arranged by size: 2017 2007 859 688 381 184 54 MscI TGG'CCA Cuts at: 10 64 1489 1832 2970 10 Size: 54 1425 343 1138 3230 Fragments arranged by size: 3230 1425 1138 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4148 4507 4666 518 Size: 648 2982 359 159 2042 Fragments arranged by size: 2982 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3606 4283 5431 5676 1606 Size: 2000 677 1148 245 2120 Fragments arranged by size: 2120 2000 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5638 NdeI CA'TA_TG Cuts at: 387 387 Size: 6190 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6190 NotI GC'GGCC_GC Cuts at: 3637 3637 Size: 6190 NspI r_CATG'y Cuts at: 1132 1229 2197 2668 3548 5093 1132 Size: 97 968 471 880 1545 2229 Fragments arranged by size: 2229 1545 968 880 471 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6190 PciI A'CATG_T Cuts at: 5089 5089 Size: 6190 PflMI CCAn_nnn'nTGG Cuts at: 1108 2504 1108 Size: 1396 4794 PmeI GTTT'AAAC Cuts at: 3494 3494 Size: 6190 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4259 4291 5772 5804 4259 Size: 32 1481 32 4645 Fragments arranged by size: 4645 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6190 PsiI TTA'TAA Cuts at: 3760 3760 Size: 6190 PstI C_TGCA'G Cuts at: 830 3542 830 Size: 2712 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6190 SalI G'TCGA_C Cuts at: 1936 3532 1936 Size: 1596 4594 SapI GCTCTTCn'nnn_ Cuts at: 3119 3291 3119 Size: 172 6018 SbfI CC_TGCA'GG Cuts at: 3542 3542 Size: 6190 ScaI AGT'ACT Cuts at: 4438 6150 4438 Size: 1712 4478 SfcI C'TryA_G Cuts at: 826 1045 2701 2995 3538 4673 5354 5545 826 Size: 219 1656 294 543 1135 681 191 1471 Fragments arranged by size: 1656 1471 1135 681 543 294 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6190 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6190 SmaI CCC'GGG Cuts at: 1872 3517 1872 Size: 1645 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4294 5195 5457 820 Size: 197 926 2351 901 262 1553 Fragments arranged by size: 2351 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3981 493 Size: 3488 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6190 SphI G_CATG'C Cuts at: 1132 3548 1132 Size: 2416 3774 SspI AAT'ATT Cuts at: 5 52 2381 4114 5 Size: 47 2329 1733 2081 Fragments arranged by size: 2329 2081 1733 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6190 StyI C'CwwG_G Cuts at: 513 1065 3629 513 Size: 552 2564 3074 Fragments arranged by size: 3074 2564 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4217 4234 4387 4572 5017 325 Size: 1255 2637 17 153 185 445 1498 Fragments arranged by size: 2637 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2064 4436 6148 Size: 274 80 33 51 1443 87 2372 1712 Cuts at: 6148 96 Size: 138 Fragments arranged by size: 2372 1712 1443 274 138 87 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2242 2274 2500 2532 6150 6182 2242 Size: 32 226 32 3618 32 2250 Fragments arranged by size: 3618 2250 226 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6190 XbaI T'CTAG_A Cuts at: 3526 3526 Size: 6190 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3062 3062 Size: 6190 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6190 XmaI C'CCGG_G Cuts at: 1870 3515 1870 Size: 1645 4545 XmnI GAAnn'nnTTC Cuts at: 2981 4319 2981 Size: 1338 4852 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3998 276 Size: 53 83 186 936 2464 2468 Fragments arranged by size: 2468 2464 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI