Restriction map for
pFN21AA1271
##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BciVI BclI BfrI
BglI BglII BlnI BsaBI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MseI
MunI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI SacI
SacII SalI SapI SbfI SexAI
SfiI SfuI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tth111I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccII 252 CGCG CG^CG
AcsI 465 GAATTC [GA]^AATT[CT]
ApoI 465 GAATTC [GA]^AATT[CT]
AspHI 1102 GTGCTC G[AT]GC[AT]^C
AvaI 1296 CTCGGG C^[CT]CG[GA]G
BamHI 1400 GGATCC G^GATCC
BbrPI 1405 CACGTG CAC^GTG
BbsI 13 GAAGAC GAAGAC(2/6)
BbvCI 558 CCTCAGC CCTCAGC(-5/-2)
BcgI 1308 CGAGGATCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 13 GAAGAC GAAGAC(2/6)
BsaAI 1405 CACGTG [CT]AC^GT[GA]
BsaWI 1493 ACCGGA [AT]^CCGG[AT]
BsiEI 1491 CGACCG CG[GA][CT]^CG
BsiHKAI 1102 GTGCTC G[AT]GC[AT]^C
BsoBI 1296 CTCGGG C^[CT]CG[GA]G
BstUI 252 CGCG CG^CG
CpoI 299 CGGACCG CG^G[AT]CCG
EarI 172 CTCTTC CTCTTC(1/4)
EcoRI 465 GAATTC G^AATTC
Ksp632I 172 CTCTTC CTCTTC(1/4)
MaeII 1406 ACGT A^CGT
MvnI 252 CGCG CG^CG
NaeI 1183 GCCGGC GCC^GGC
NcoI 1356 CCATGG C^CATGG
NgoMIV 1183 GCCGGC G^CCGGC
PmaCI 1405 CACGTG CAC^GTG
PmlI 1405 CACGTG CAC^GTG
PvuII 393 CAGCTG CAG^CTG
RsrII 299 CGGACCG CG^G[AT]CCG
ScaI 209 AGTACT AGT^ACT
SgrAI 1182 CGCCGGCG C[GA]^CCGG[CT]G
XcmI 1328 CCAGCACCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 271 GTCTAC GT^[AC][GT]AC
1282 GTAGAC
ApaI 352 GGGCCC GGGCC^C
1348 GGGCCC
BbeI 444 GGCGCC GGCGC^C
1186 GGCGCC
BlpI 489 GCTCAGC GC^T[ACGT]AGC
1202 GCTCAGC
Bpu1102I 489 GCTCAGC GC^T[ACGT]AGC
1202 GCTCAGC
BsrDI 216 CATTGC GCAATG(2/0)
59 GCAATG
BsrFI 1183 GCCGGC [GA]^CCGG[CT]
1604 ACCGGC
BstYI 1311 GGATCT [GA]^GATC[CT]
1400 GGATCC
CelII 489 GCTCAGC GC^T[ACGT]AGC
1202 GCTCAGC
Cfr10I 1183 GCCGGC [GA]^CCGG[CT]
1604 ACCGGC
DsaI 651 CCGTGG C^C[GA][CT]GG
1356 CCATGG
HaeII 444 GGCGCC [GA]GCGC^[CT]
1186 GGCGCC
HgaI 486 GACGC GACGC(5/10)
1611 GCGTC
KasI 444 GGCGCC G^GCGCC
1186 GGCGCC
MboII 13 GAAGA GAAGA(8/7)
173 TCTTC
MflI 1311 GGATCT [GA]^GATC[CT]
1400 GGATCC
MslI 900 CACCTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1107 CACCAAGGTG
NarI 444 GGCGCC GG^CGCC
1186 GGCGCC
PleI 451 GAGTC GAGTC(4/5)
535 GAGTC
SfoI 444 GGCGCC GGC^GCC
1186 GGCGCC
TaqI 120 TCGA T^CGA
1307 TCGA
TfiI 78 GATTC G^A[AT]TC
513 GAATC
TthHB8I 120 TCGA T^CGA
1307 TCGA
XhoII 1311 GGATCT [GA]^GATC[CT]
1400 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeIII MboI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TseI Tsp45I Tsp509I
TspRI Van91I