(Circular) (Six-base) MAPSORT of: pFN21AA1270.seq Check: 6848 from: 1 to: 7660 pFN21AA1270 7660 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3921 3921 Size: 7660 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5470 278 Size: 53 83 186 936 3934 2468 Fragments arranged by size: 3934 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3620 5003 1937 Size: 1683 1383 4594 Fragments arranged by size: 4594 1683 1383 Acc65I G'GTAC_C Cuts at: 3130 4981 3130 Size: 1851 5809 AclI AA'CG_TT Cuts at: 1563 5787 6160 1563 Size: 4224 373 3063 Fragments arranged by size: 4224 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3273 3327 3705 5723 7107 7375 1632 Size: 1641 54 378 2018 1384 268 1917 Fragments arranged by size: 2018 1917 1641 1384 378 268 54 AfeI AGC'GCT Cuts at: 3665 3665 Size: 7660 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7463 AflIII A'CryG_T Cuts at: 1203 2305 6559 7536 1203 Size: 1102 4254 977 1327 Fragments arranged by size: 4254 1327 1102 977 AgeI A'CCGG_T Cuts at: 6491 6491 Size: 7660 AhdI GACnn_n'nnGTC Cuts at: 6389 6389 Size: 7660 AleI CACnn'nnGTG Cuts at: 1166 4736 1166 Size: 3570 4090 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4581 4754 1184 Size: 3397 173 4090 Fragments arranged by size: 4090 3397 173 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3749 3781 3749 Size: 32 7628 AlwNI CAG_nnn'CTG Cuts at: 2779 3003 3940 4163 4390 4624 4670 5403 6975 Size: 224 937 223 227 234 46 733 1572 Cuts at: 6975 2779 Size: 3464 Fragments arranged by size: 3464 1572 937 733 234 227 224 223 46 ApaI G_GGCC'C Cuts at: 2664 3784 3947 2664 Size: 1120 163 6377 Fragments arranged by size: 6377 1120 163 ApaLI G'TGCA_C Cuts at: 5717 6873 5717 Size: 1156 6504 ApoI r'AATT_y Cuts at: 1493 4969 5195 5347 7456 1493 Size: 3476 226 152 2109 1697 Fragments arranged by size: 3476 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6214 160 Size: 872 5182 1606 Fragments arranged by size: 5182 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7660 AvaI C'yCGr_G Cuts at: 1870 1943 3777 4371 4985 1870 Size: 73 1834 594 614 4545 Fragments arranged by size: 4545 1834 614 594 73 AvrII C'CTAG_G Cuts at: 3118 3118 Size: 7660 BamHI G'GATC_C Cuts at: 2275 2487 3060 4990 5357 2275 Size: 212 573 1930 367 4578 Fragments arranged by size: 4578 1930 573 367 212 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2179 2267 2587 2908 3103 Size: 325 217 627 392 88 320 321 195 Cuts at: 3103 3130 3590 4099 4186 4799 4892 4981 6436 Size: 27 460 509 87 613 93 89 1455 Cuts at: 6436 618 Size: 1842 Fragments arranged by size: 1842 1455 627 613 509 460 392 325 321 320 217 195 93 89 88 87 27 BanII G_rGCy'C Cuts at: 1391 2106 2664 2707 3784 3947 3968 4874 4979 Size: 715 558 43 1077 163 21 906 105 Cuts at: 4979 1391 Size: 4072 Fragments arranged by size: 4072 1077 906 715 558 163 105 43 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2728 3013 928 Size: 971 829 285 5575 Fragments arranged by size: 5575 971 829 285 BbvCI CC'TCA_GC Cuts at: 2078 4949 2078 Size: 2871 4789 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5851 5885 1903 Size: 34 3914 34 3678 Fragments arranged by size: 3914 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2638 5552 6762 2638 Size: 2914 1210 3536 Fragments arranged by size: 3536 2914 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7660 BfrBI ATG'CAT Cuts at: 4202 4202 Size: 7660 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2068 2132 2788 Size: 107 122 71 631 605 396 64 656 Cuts at: 2788 3230 4367 4519 6271 136 Size: 442 1137 152 1752 1525 Fragments arranged by size: 1752 1525 1137 656 631 605 442 396 152 122 107 71 64 BglII A'GATC_T Cuts at: 3093 7655 3093 Size: 4562 3098 BlpI GC'TnA_GC Cuts at: 2576 3456 5077 2576 Size: 880 1621 5159 Fragments arranged by size: 5159 1621 880 Bme1580I G_kGCm'C Cuts at: 1790 2184 2270 2664 2911 3448 3528 3784 3928 Size: 394 86 394 247 537 80 256 144 Cuts at: 3928 3947 4104 4191 4328 4834 5721 6877 1790 Size: 19 157 87 137 506 887 1156 2573 Fragments arranged by size: 2573 1156 887 537 506 394 394 256 247 157 144 137 87 86 80 19 BmgBI CAC'GTC Cuts at: 1318 2306 3760 4734 1318 Size: 988 1454 974 4244 Fragments arranged by size: 4244 1454 988 974 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2208 3161 3764 Size: 416 53 83 399 24 791 953 603 Cuts at: 3764 3931 4284 4359 4510 5391 6349 442 Size: 167 353 75 151 881 958 1753 Fragments arranged by size: 1753 958 953 881 791 603 416 399 353 167 151 83 75 53 24 BmtI G_CTAG'C Cuts at: 1056 2598 1056 Size: 1542 6118 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4654 4699 4654 Size: 45 7615 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2358 2576 3260 3395 3431 4649 6320 1239 Size: 1119 218 684 135 36 1218 1671 2579 Fragments arranged by size: 2579 1671 1218 1119 684 218 135 36 Bpu10I CC'TnA_GC Cuts at: 2078 2581 3981 4949 2078 Size: 503 1400 968 4789 Fragments arranged by size: 4789 1400 968 503 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5785 6650 6948 5785 Size: 865 298 6497 Fragments arranged by size: 6497 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2367 2552 3330 6323 882 Size: 617 868 185 778 2993 2219 Fragments arranged by size: 2993 2219 868 778 617 185 BsaAI yAC'GTr Cuts at: 493 1204 4271 5451 493 Size: 711 3067 1180 2702 Fragments arranged by size: 3067 2702 1180 711 BsaBI GATnn'nnATC Cuts at: 3162 7654 3162 Size: 4492 3168 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3018 3877 5467 5849 Size: 53 83 186 936 1485 859 1590 382 Cuts at: 5849 6484 275 Size: 635 1451 Fragments arranged by size: 1590 1485 1451 936 859 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4895 6093 6491 6765 6912 7323 4895 Size: 1198 398 274 147 411 5232 Fragments arranged by size: 5232 1198 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3542 3572 4199 4229 1178 Size: 30 165 30 2139 30 627 30 4609 Fragments arranged by size: 4609 2139 627 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2456 3392 3443 3665 4109 1186 Size: 1270 936 51 222 444 4737 Fragments arranged by size: 4737 1270 936 444 222 51 BseYI C'CCAG_C Cuts at: 2709 2784 3435 3569 4210 4362 4727 4755 6863 Size: 75 651 134 641 152 365 28 2108 Cuts at: 6863 7359 2709 Size: 496 3010 Fragments arranged by size: 3010 2108 651 641 496 365 152 134 75 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3872 4076 4864 1163 Size: 573 2136 204 788 3959 Fragments arranged by size: 3959 2136 788 573 204 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2090 2127 5110 5871 6899 1540 Size: 275 186 89 37 2983 761 1028 2301 Fragments arranged by size: 2983 2301 1028 761 275 186 89 37 BsiHKAI G_wGCw'C Cuts at: 4743 5721 5806 6877 4743 Size: 978 85 1071 5526 Fragments arranged by size: 5526 1071 978 85 BsmI GAATG_Cn' Cuts at: 5171 5264 5171 Size: 93 7567 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2184 2270 2664 2707 2911 3448 Size: 399 316 78 86 394 43 204 537 Cuts at: 3448 3528 3784 3928 3947 3968 4104 4191 4328 Size: 80 256 144 19 21 136 87 137 Cuts at: 4328 4743 4834 4874 4979 5721 5806 6877 1391 Size: 415 91 40 105 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 537 415 399 394 316 256 204 144 137 136 105 91 87 86 85 80 78 43 40 21 19 BspEI T'CCGG_A Cuts at: 7323 7323 Size: 7660 BspHI T'CATG_A Cuts at: 5549 7279 5549 Size: 1730 5930 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3921 5015 844 Size: 3077 1094 3489 Fragments arranged by size: 3489 3077 1094 BsrBI CCG'CTC Cuts at: 1394 1823 2092 3244 3415 4420 4633 5547 1394 Size: 429 269 1152 171 1005 213 914 3507 Fragments arranged by size: 3507 1152 1005 914 429 269 213 171 BsrDI GCAATG_nn' Cuts at: 65 1248 6155 6329 65 Size: 1183 4907 174 1396 Fragments arranged by size: 4907 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2172 6304 6491 1700 Size: 110 362 4132 187 2869 Fragments arranged by size: 4132 2869 362 187 110 BsrGI T'GTAC_A Cuts at: 96 4086 96 Size: 3990 3670 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7660 BssSI C'ACGA_G Cuts at: 5720 6732 5720 Size: 1012 6648 BstAPI GCAn_nnn'nTGC Cuts at: 794 2779 794 Size: 1985 5675 BstBI TT'CG_AA Cuts at: 2053 6464 2053 Size: 4411 3249 BstEII G'GTnAC_C Cuts at: 1184 2525 1184 Size: 1341 6319 BstXI CCAn_nnnn'nTGG Cuts at: 3500 3668 3791 4127 3500 Size: 168 123 336 7033 Fragments arranged by size: 7033 336 168 123 BstYI r'GATC_y Cuts at: 1972 2275 2487 2508 2673 3060 3093 4990 5357 Size: 303 212 21 165 387 33 1897 367 Cuts at: 5357 5742 5759 7211 7297 7309 7655 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1897 1452 387 385 367 346 303 212 165 86 33 21 17 12 BstZ17I GTA'TAC Cuts at: 3621 3621 Size: 7660 Bsu36I CC'TnA_GG Cuts at: 2493 2493 Size: 7660 BtgI C'CryG_G Cuts at: 513 1065 2004 2164 2203 2271 3087 3648 3727 Size: 552 939 160 39 68 816 561 79 Cuts at: 3727 4065 4191 4761 513 Size: 338 126 570 3412 Fragments arranged by size: 3412 939 816 570 561 552 338 160 126 79 68 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6921 Fragments arranged by size: 6921 683 56 BtsI GCAGTG_nn' Cuts at: 3645 4047 4113 4226 4572 4845 5176 5970 5990 Size: 402 66 113 346 273 331 794 20 Cuts at: 5990 3645 Size: 5315 Fragments arranged by size: 5315 794 402 346 331 273 113 66 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7660 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7625 DraI TTT'AAA Cuts at: 4964 4964 Size: 7660 DraIII CAC_nnn'GTG Cuts at: 2999 3332 2999 Size: 333 7327 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6667 809 Size: 507 5351 1802 Fragments arranged by size: 5351 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2087 2124 2167 Size: 54 1425 50 275 18 257 37 43 Cuts at: 2167 2310 3903 4341 4410 4539 5107 5996 7625 Size: 143 1593 438 69 129 568 889 1629 Cuts at: 7625 8 Size: 43 Fragments arranged by size: 1629 1593 1425 889 568 438 275 257 143 129 69 54 50 43 43 37 18 EagI C'GGCC_G Cuts at: 1537 1812 2087 2124 5107 1537 Size: 275 275 37 2983 4090 Fragments arranged by size: 4090 2983 275 275 37 EarI CTCTTCn'nnn_ Cuts at: 1353 5590 1353 Size: 4237 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3268 4463 6247 6621 6767 387 Size: 1064 138 1679 1195 1784 374 146 1280 Fragments arranged by size: 1784 1679 1280 1195 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2358 2576 3260 3273 3327 3395 3431 Size: 393 726 218 684 13 54 68 36 Cuts at: 3431 3705 4649 5723 6320 7107 7375 1239 Size: 274 944 1074 597 787 268 1524 Fragments arranged by size: 1524 1074 944 787 726 684 597 393 274 268 218 68 54 36 13 EcoNI CCTnn'n_nnAGG Cuts at: 3809 4394 3809 Size: 585 7075 EcoO109I rG'GnC_Cy Cuts at: 2074 2661 3038 3276 3284 3944 4123 4288 4646 Size: 587 377 238 8 660 179 165 358 Cuts at: 4646 4723 2074 Size: 77 5011 Fragments arranged by size: 5011 660 587 377 358 238 179 165 77 8 EcoRI G'AATT_C Cuts at: 4969 5347 4969 Size: 378 7282 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2275 2275 Size: 7660 FspI TGC'GCA Cuts at: 6166 6166 Size: 7660 HaeII r_GCGC'y Cuts at: 1678 3517 3667 4112 6807 1678 Size: 1839 150 445 2695 2531 Fragments arranged by size: 2695 2531 1839 445 150 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3022 3052 3054 3084 3247 Size: 32 135 32 1450 30 2 30 163 Cuts at: 3247 3279 3786 3818 4199 4231 4652 4684 6301 Size: 32 507 32 381 32 421 32 1617 Cuts at: 6301 6333 6375 6407 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1617 1450 507 421 381 163 135 42 32 32 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 5004 5250 669 Size: 988 281 3066 246 3079 Fragments arranged by size: 3079 3066 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7660 HpaI GTT'AAC Cuts at: 5250 5250 Size: 7660 KpnI G_GTAC'C Cuts at: 3134 4985 3134 Size: 1851 5809 MfeI C'AATT_G Cuts at: 2410 5259 2410 Size: 2849 4811 MluI A'CGCG_T Cuts at: 7536 7536 Size: 7660 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2326 3038 6774 6958 1305 Size: 1021 712 3736 184 2007 Fragments arranged by size: 3736 2007 1021 712 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2169 2312 4541 10 Size: 54 1425 343 337 143 2229 3129 Fragments arranged by size: 3129 2229 1425 343 337 143 54 MslI CAynn'nnrTG Cuts at: 518 1166 2804 3864 4736 5618 5977 6136 518 Size: 648 1638 1060 872 882 359 159 2042 Fragments arranged by size: 2042 1638 1060 882 872 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2024 2205 2713 2788 2878 3046 3404 3573 Size: 418 181 508 75 90 168 358 169 Cuts at: 3573 3856 3988 4525 4561 4711 5076 5753 6901 Size: 283 132 537 36 150 365 677 1148 Cuts at: 6901 7146 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 537 508 418 365 358 283 245 181 169 168 150 132 90 75 36 NcoI C'CATG_G Cuts at: 513 1065 2004 2164 3087 4761 513 Size: 552 939 160 923 1674 3412 Fragments arranged by size: 3412 1674 939 923 552 160 NdeI CA'TA_TG Cuts at: 387 387 Size: 7660 NheI G'CTAG_C Cuts at: 1052 2594 1052 Size: 1542 6118 NotI GC'GGCC_GC Cuts at: 5107 5107 Size: 7660 NruI TCG'CGA Cuts at: 4876 4876 Size: 7660 NsiI A_TGCA'T Cuts at: 4204 4204 Size: 7660 NspI r_CATG'y Cuts at: 1132 1229 2795 3863 5018 6563 1132 Size: 97 1566 1068 1155 1545 2229 Fragments arranged by size: 2229 1566 1545 1155 1068 97 PasI CC'CwG_GG Cuts at: 1348 3026 3280 3940 4795 1348 Size: 1678 254 660 855 4213 Fragments arranged by size: 4213 1678 855 660 254 PciI A'CATG_T Cuts at: 6559 6559 Size: 7660 PflMI CCAn_nnn'nTGG Cuts at: 1108 2176 4204 4390 4762 1108 Size: 1068 2028 186 372 4006 Fragments arranged by size: 4006 2028 1068 372 186 PmeI GTTT'AAAC Cuts at: 4964 4964 Size: 7660 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5729 5761 7242 7274 5729 Size: 32 1481 32 6115 Fragments arranged by size: 6115 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3038 3276 4123 4288 3038 Size: 238 847 165 6410 Fragments arranged by size: 6410 847 238 165 PshAI GACnn'nnGTC Cuts at: 1613 2477 1613 Size: 864 6796 PsiI TTA'TAA Cuts at: 5230 5230 Size: 7660 PspOMI G'GGCC_C Cuts at: 2660 3780 3943 2660 Size: 1120 163 6377 Fragments arranged by size: 6377 1120 163 PstI C_TGCA'G Cuts at: 830 2029 2113 2775 2906 3513 4233 4560 5012 Size: 1199 84 662 131 607 720 327 452 Cuts at: 5012 830 Size: 3478 Fragments arranged by size: 3478 1199 720 662 607 452 327 131 84 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7660 PvuII CAG'CTG Cuts at: 2024 2713 3856 3988 4711 2024 Size: 689 1143 132 723 4973 Fragments arranged by size: 4973 1143 723 689 132 SacII CC_GC'GG Cuts at: 2206 2206 Size: 7660 SalI G'TCGA_C Cuts at: 1936 5002 1936 Size: 3066 4594 SbfI CC_TGCA'GG Cuts at: 5012 5012 Size: 7660 ScaI AGT'ACT Cuts at: 5908 7620 5908 Size: 1712 5948 SexAI A'CCwGG_T Cuts at: 2730 3201 3735 3846 4290 2730 Size: 471 534 111 444 6100 Fragments arranged by size: 6100 534 471 444 111 SfcI C'TryA_G Cuts at: 826 1045 2025 2109 2771 2902 3135 3509 4229 Size: 219 980 84 662 131 233 374 720 Cuts at: 4229 4281 4556 5008 6143 6824 7015 826 Size: 52 275 452 1135 681 191 1471 Fragments arranged by size: 1471 1135 980 720 681 662 452 374 275 233 219 191 131 84 52 SfiI GGCCn_nnn'nGGCC Cuts at: 2132 4367 2132 Size: 2235 5425 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7660 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7660 SmaI CCC'GGG Cuts at: 1872 3779 4987 1872 Size: 1907 1208 4545 Fragments arranged by size: 4545 1907 1208 SmlI C'TyrA_G Cuts at: 820 1017 1943 5764 6665 6927 820 Size: 197 926 3821 901 262 1553 Fragments arranged by size: 3821 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4271 5451 493 Size: 3778 1180 2702 Fragments arranged by size: 3778 2702 1180 SpeI A'CTAG_T Cuts at: 152 152 Size: 7660 SphI G_CATG'C Cuts at: 1132 5018 1132 Size: 3886 3774 SrfI GCCC'GGGC Cuts at: 3779 3779 Size: 7660 SspI AAT'ATT Cuts at: 5 52 2432 5584 5 Size: 47 2380 3152 2081 Fragments arranged by size: 3152 2380 2081 47 StuI AGG'CCT Cuts at: 1517 2120 3872 4826 1517 Size: 603 1752 954 4351 Fragments arranged by size: 4351 1752 954 603 StyI C'CwwG_G Cuts at: 513 1065 2004 2115 2164 2532 3087 3118 3351 Size: 552 939 111 49 368 555 31 233 Cuts at: 3351 3517 4320 4467 4761 4888 5099 513 Size: 166 803 147 294 127 211 3074 Fragments arranged by size: 3074 939 803 555 552 368 294 233 211 166 147 127 111 49 31 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3923 4022 5687 5704 5857 6042 6487 Size: 1255 2343 99 1665 17 153 185 445 Cuts at: 6487 325 Size: 1498 Fragments arranged by size: 2343 1665 1498 1255 445 185 153 99 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2666 3549 4086 Size: 274 80 33 51 1443 689 883 537 Cuts at: 4086 5906 7618 96 Size: 1820 1712 138 Fragments arranged by size: 1820 1712 1443 883 689 537 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2260 2292 3099 3131 7620 7652 2260 Size: 32 807 32 4489 32 2268 Fragments arranged by size: 4489 2268 807 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4664 1316 Size: 3348 4312 XbaI T'CTAG_A Cuts at: 4996 4996 Size: 7660 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2539 2539 Size: 7660 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7660 XmaI C'CCGG_G Cuts at: 1870 3777 4985 1870 Size: 1907 1208 4545 Fragments arranged by size: 4545 1907 1208 XmnI GAAnn'nnTTC Cuts at: 2246 5789 2246 Size: 3543 4117 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5468 276 Size: 53 83 186 936 3934 2468 Fragments arranged by size: 3934 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BbeI BsiWI BsmBI EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV PacI PfoI PmlI PspXI PsrI RsrII SacI SanDI SapI SfoI SwaI