(Circular) (Six-base) MAPSORT of: pFN21AA1261.seq Check: 4042 from: 1 to: 7024 pFN21AA1261 7024 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2350 2350 Size: 7024 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4834 278 Size: 53 83 186 936 3298 2468 Fragments arranged by size: 3298 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4367 1937 Size: 2430 4594 Acc65I G'GTAC_C Cuts at: 4345 4345 Size: 7024 AclI AA'CG_TT Cuts at: 1563 5151 5524 1563 Size: 3588 373 3063 Fragments arranged by size: 3588 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2171 2445 2604 2727 5087 6471 6739 1632 Size: 539 274 159 123 2360 1384 268 1917 Fragments arranged by size: 2360 1917 1384 539 274 268 159 123 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6827 AflIII A'CryG_T Cuts at: 1203 3505 3507 5923 6900 1203 Size: 2302 2 2416 977 1327 Fragments arranged by size: 2416 2302 1327 977 2 AgeI A'CCGG_T Cuts at: 5855 5855 Size: 7024 AhdI GACnn_n'nnGTC Cuts at: 5753 5753 Size: 7024 AleI CACnn'nnGTG Cuts at: 1166 3294 1166 Size: 2128 4896 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2079 1184 Size: 895 6129 AlwNI CAG_nnn'CTG Cuts at: 3224 3939 4767 6339 3224 Size: 715 828 1572 3909 Fragments arranged by size: 3909 1572 828 715 ApaLI G'TGCA_C Cuts at: 2289 5081 6237 2289 Size: 2792 1156 3076 Fragments arranged by size: 3076 2792 1156 ApoI r'AATT_y Cuts at: 1493 2078 4333 4559 4711 6820 1493 Size: 585 2255 226 152 2109 1697 Fragments arranged by size: 2255 2109 1697 585 226 152 AseI AT'TA_AT Cuts at: 160 1032 5578 160 Size: 872 4546 1606 Fragments arranged by size: 4546 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7024 AvaI C'yCGr_G Cuts at: 1870 1943 2802 4349 1870 Size: 73 859 1547 4545 Fragments arranged by size: 4545 1547 859 73 BamHI G'GATC_C Cuts at: 2788 4354 4721 2788 Size: 1566 367 5091 Fragments arranged by size: 5091 1566 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2050 3896 4345 5800 618 Size: 325 217 627 263 1846 449 1455 1842 Fragments arranged by size: 1846 1842 1455 627 449 325 263 217 BanII G_rGCy'C Cuts at: 1391 2469 2618 2813 3192 4343 1391 Size: 1078 149 195 379 1151 4072 Fragments arranged by size: 4072 1151 1078 379 195 149 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2189 928 Size: 971 290 5763 Fragments arranged by size: 5763 971 290 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5215 5249 1903 Size: 34 3278 34 3678 Fragments arranged by size: 3678 3278 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2635 4916 6126 2635 Size: 2281 1210 3533 Fragments arranged by size: 3533 2281 1210 BclI T'GATC_A Cuts at: 1548 2584 1548 Size: 1036 5988 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3558 5635 136 Size: 107 122 71 631 605 1886 2077 1525 Fragments arranged by size: 2077 1886 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3990 7019 3990 Size: 3029 3995 BlpI GC'TnA_GC Cuts at: 4241 4441 4241 Size: 200 6824 Bme1580I G_kGCm'C Cuts at: 1790 2293 3138 5085 6241 1790 Size: 503 845 1947 1156 2573 Fragments arranged by size: 2573 1947 1156 845 503 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7024 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4755 5713 442 Size: 416 53 83 399 24 3338 958 1753 Fragments arranged by size: 3338 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7024 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2387 2453 2506 4038 4207 5684 1239 Size: 1148 66 53 1532 169 1477 2579 Fragments arranged by size: 2579 1532 1477 1148 169 66 53 Bpu10I CC'TnA_GC Cuts at: 2018 2018 Size: 7024 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2843 3061 4109 5149 6014 6312 2843 Size: 218 1048 1040 865 298 3555 Fragments arranged by size: 3555 1048 1040 865 298 218 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2438 5687 882 Size: 617 939 3249 2219 Fragments arranged by size: 3249 2219 939 617 BsaAI yAC'GTr Cuts at: 493 1204 3508 4815 493 Size: 711 2304 1307 2702 Fragments arranged by size: 2702 2304 1307 711 BsaBI GATnn'nnATC Cuts at: 7018 7018 Size: 7024 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3405 4831 5213 5848 Size: 53 83 186 936 1872 1426 382 635 Cuts at: 5848 275 Size: 1451 Fragments arranged by size: 1872 1451 1426 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5457 5855 6129 6276 6687 5457 Size: 398 274 147 411 5794 Fragments arranged by size: 5794 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2418 2448 2588 2618 2651 Size: 30 165 30 1015 30 140 30 33 Cuts at: 2651 2681 2968 2998 3185 3215 3458 3488 3968 Size: 30 287 30 187 30 243 30 480 Cuts at: 3968 3998 1178 Size: 30 4204 Fragments arranged by size: 4204 1015 480 287 243 187 165 140 33 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2671 2905 1186 Size: 1485 234 5305 Fragments arranged by size: 5305 1485 234 BseYI C'CCAG_C Cuts at: 2409 2812 3185 3579 6227 6723 2409 Size: 403 373 394 2648 496 2710 Fragments arranged by size: 2710 2648 496 403 394 373 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2357 3806 1163 Size: 573 621 1449 4381 Fragments arranged by size: 4381 1449 621 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4474 5235 6263 1540 Size: 275 186 2473 761 1028 2301 Fragments arranged by size: 2473 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2293 2322 2530 2618 5085 5170 6241 2293 Size: 29 208 88 2467 85 1071 3076 Fragments arranged by size: 3076 2467 1071 208 88 85 29 BsmI GAATG_Cn' Cuts at: 2284 3327 4186 4535 4628 2284 Size: 1043 859 349 93 4680 Fragments arranged by size: 4680 1043 859 349 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2968 2968 Size: 7024 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2293 2322 2469 2530 2618 2813 3138 Size: 399 503 29 147 61 88 195 325 Cuts at: 3138 3192 4343 5085 5170 6241 1391 Size: 54 1151 742 85 1071 2174 Fragments arranged by size: 2174 1151 1071 742 503 399 325 195 147 88 85 61 54 29 BspEI T'CCGG_A Cuts at: 6687 6687 Size: 7024 BspHI T'CATG_A Cuts at: 4102 4913 6643 4102 Size: 811 1730 4483 Fragments arranged by size: 4483 1730 811 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2350 3954 4379 844 Size: 1506 1604 425 3489 Fragments arranged by size: 3489 1604 1506 425 BsrBI CCG'CTC Cuts at: 1394 1823 3176 4911 1394 Size: 429 1353 1735 3507 Fragments arranged by size: 3507 1735 1353 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4057 5519 5693 65 Size: 1183 2809 1462 174 1396 Fragments arranged by size: 2809 1462 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5668 5855 1700 Size: 110 3858 187 2869 Fragments arranged by size: 3858 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3510 96 Size: 3414 3610 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7024 BssSI C'ACGA_G Cuts at: 3499 5084 6096 3499 Size: 1585 1012 4427 Fragments arranged by size: 4427 1585 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3224 794 Size: 2430 4594 BstBI TT'CG_AA Cuts at: 5828 5828 Size: 7024 BstEII G'GTnAC_C Cuts at: 1184 2362 3481 1184 Size: 1178 1119 4727 Fragments arranged by size: 4727 1178 1119 BstXI CCAn_nnnn'nTGG Cuts at: 3863 3863 Size: 7024 BstYI r'GATC_y Cuts at: 1972 2788 3817 3990 4354 4721 5106 5123 6575 Size: 816 1029 173 364 367 385 17 1452 Cuts at: 6575 6661 6673 7019 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1029 816 385 367 364 346 173 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3083 3083 Size: 7024 BtgI C'CryG_G Cuts at: 513 1065 2367 3460 4257 513 Size: 552 1302 1093 797 3280 Fragments arranged by size: 3280 1302 1093 797 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2754 3790 487 Size: 683 56 1528 1036 3721 Fragments arranged by size: 3721 1528 1036 683 56 BtsI GCAGTG_nn' Cuts at: 2506 4038 4540 5334 5354 2506 Size: 1532 502 794 20 4176 Fragments arranged by size: 4176 1532 794 502 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7024 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6989 DraI TTT'AAA Cuts at: 2077 4328 2077 Size: 2251 4773 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3929 6031 809 Size: 507 2613 2102 1802 Fragments arranged by size: 2613 2102 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3753 4471 5360 Size: 54 1425 50 275 18 1923 718 889 Cuts at: 5360 6989 8 Size: 1629 43 Fragments arranged by size: 1923 1629 1425 889 718 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4471 1537 Size: 275 2659 4090 Fragments arranged by size: 4090 2659 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2109 2623 4954 1353 Size: 756 514 2331 3423 Fragments arranged by size: 3423 2331 756 514 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5611 5985 6131 387 Size: 1064 138 4022 374 146 1280 Fragments arranged by size: 4022 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2616 2616 Size: 7024 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2171 2387 2445 2453 2506 2604 2727 Size: 393 539 216 58 8 53 98 123 Cuts at: 2727 4038 4207 5087 5684 6471 6739 1239 Size: 1311 169 880 597 787 268 1524 Fragments arranged by size: 1524 1311 880 787 597 539 393 268 216 169 123 98 58 53 8 EcoNI CCTnn'n_nnAGG Cuts at: 3838 3950 3838 Size: 112 6912 EcoO109I rG'GnC_Cy Cuts at: 3087 3685 3936 3943 3087 Size: 598 251 7 6168 Fragments arranged by size: 6168 598 251 7 EcoRI G'AATT_C Cuts at: 4333 4711 4333 Size: 378 6646 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3796 3796 Size: 7024 FspI TGC'GCA Cuts at: 5530 5530 Size: 7024 HaeII r_GCGC'y Cuts at: 1678 6171 1678 Size: 4493 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2418 2450 2588 2592 2620 Size: 32 135 32 846 32 138 4 28 Cuts at: 2620 2624 2666 2698 2777 2809 3408 3440 3710 Size: 4 42 32 79 32 599 32 270 Cuts at: 3710 3742 3968 3982 4000 4014 5665 5697 5739 Size: 32 226 14 18 14 1651 32 42 Cuts at: 5739 5771 1373 Size: 32 2626 Fragments arranged by size: 2626 1651 846 599 270 226 138 135 79 42 42 32 32 32 32 32 32 32 32 32 28 18 14 14 4 4 HincII GTy'rAC Cuts at: 669 1657 1938 2773 3064 4368 4614 669 Size: 988 281 835 291 1304 246 3079 Fragments arranged by size: 3079 1304 988 835 291 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7024 HpaI GTT'AAC Cuts at: 4614 4614 Size: 7024 KpnI G_GTAC'C Cuts at: 4349 4349 Size: 7024 MfeI C'AATT_G Cuts at: 4623 4623 Size: 7024 MluI A'CGCG_T Cuts at: 6900 6900 Size: 7024 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3140 3634 3722 6138 6322 1305 Size: 1835 494 88 2416 184 2007 Fragments arranged by size: 2416 2007 1835 494 184 88 MscI TGG'CCA Cuts at: 10 64 1489 1832 3755 10 Size: 54 1425 343 1923 3279 Fragments arranged by size: 3279 1923 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2427 2580 3294 3296 3809 3861 4101 Size: 648 1261 153 714 2 513 52 240 Cuts at: 4101 4256 4982 5341 5500 518 Size: 155 726 359 159 2042 Fragments arranged by size: 2042 1261 726 714 648 513 359 240 159 155 153 52 2 MspA1I CmG'CkG Cuts at: 1606 2197 3221 3236 3873 3964 4440 5117 6265 Size: 591 1024 15 637 91 476 677 1148 Cuts at: 6265 6510 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1024 677 637 591 476 245 91 15 NcoI C'CATG_G Cuts at: 513 1065 2367 513 Size: 552 1302 5170 Fragments arranged by size: 5170 1302 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7024 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7024 NotI GC'GGCC_GC Cuts at: 4471 4471 Size: 7024 NruI TCG'CGA Cuts at: 2013 2013 Size: 7024 NspI r_CATG'y Cuts at: 1132 1229 3295 3437 4070 4382 5927 1132 Size: 97 2066 142 633 312 1545 2229 Fragments arranged by size: 2229 2066 1545 633 312 142 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7024 PciI A'CATG_T Cuts at: 5923 5923 Size: 7024 PflMI CCAn_nnn'nTGG Cuts at: 1108 3835 3906 1108 Size: 2727 71 4226 Fragments arranged by size: 4226 2727 71 PfoI T'CCnGG_A Cuts at: 3025 3331 3025 Size: 306 6718 PmeI GTTT'AAAC Cuts at: 4328 4328 Size: 7024 PmlI CAC'GTG Cuts at: 3508 3508 Size: 7024 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5093 5125 6606 6638 5093 Size: 32 1481 32 5479 Fragments arranged by size: 5479 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3087 3685 3936 3943 3087 Size: 598 251 7 6168 Fragments arranged by size: 6168 598 251 7 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7024 PsiI TTA'TAA Cuts at: 4594 4594 Size: 7024 PspXI vC'TCGA_Gb Cuts at: 2802 2802 Size: 7024 PstI C_TGCA'G Cuts at: 830 3623 3794 3969 4376 830 Size: 2793 171 175 407 3478 Fragments arranged by size: 3478 2793 407 175 171 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7024 PvuII CAG'CTG Cuts at: 3221 3873 3964 3221 Size: 652 91 6281 Fragments arranged by size: 6281 652 91 SacI G_AGCT'C Cuts at: 2618 2618 Size: 7024 SalI G'TCGA_C Cuts at: 1936 4366 1936 Size: 2430 4594 SapI GCTCTTCn'nnn_ Cuts at: 2623 2623 Size: 7024 SbfI CC_TGCA'GG Cuts at: 4376 4376 Size: 7024 ScaI AGT'ACT Cuts at: 2908 3670 5272 6984 2908 Size: 762 1602 1712 2948 Fragments arranged by size: 2948 1712 1602 762 SfcI C'TryA_G Cuts at: 826 1045 3619 3790 3965 4372 5507 6188 6379 Size: 219 2574 171 175 407 1135 681 191 Cuts at: 6379 826 Size: 1471 Fragments arranged by size: 2574 1471 1135 681 407 219 191 175 171 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7024 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7024 SmaI CCC'GGG Cuts at: 1872 4351 1872 Size: 2479 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2802 2858 3076 4124 5128 6029 Size: 197 926 859 56 218 1048 1004 901 Cuts at: 6029 6291 820 Size: 262 1553 Fragments arranged by size: 1553 1048 1004 926 901 859 262 218 197 56 SnaBI TAC'GTA Cuts at: 493 4815 493 Size: 4322 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7024 SphI G_CATG'C Cuts at: 1132 3437 4382 1132 Size: 2305 945 3774 Fragments arranged by size: 3774 2305 945 SspI AAT'ATT Cuts at: 5 52 4948 5 Size: 47 4896 2081 Fragments arranged by size: 4896 2081 47 StuI AGG'CCT Cuts at: 1517 3037 1517 Size: 1520 5504 StyI C'CwwG_G Cuts at: 513 1065 2367 3771 3834 4463 513 Size: 552 1302 1404 63 629 3074 Fragments arranged by size: 3074 1404 1302 629 552 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2503 3570 5051 5068 5221 5406 5851 Size: 1255 923 1067 1481 17 153 185 445 Cuts at: 5851 325 Size: 1498 Fragments arranged by size: 1498 1481 1255 1067 923 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2906 3510 3668 Size: 274 80 33 51 1443 929 604 158 Cuts at: 3668 5270 6982 96 Size: 1602 1712 138 Fragments arranged by size: 1712 1602 1443 929 604 274 158 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2651 2683 3185 3217 3458 3490 6984 7016 2651 Size: 32 502 32 241 32 3494 32 2659 Fragments arranged by size: 3494 2659 502 241 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2430 1316 Size: 1114 5910 XbaI T'CTAG_A Cuts at: 4360 4360 Size: 7024 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3685 4020 3685 Size: 335 6689 XhoI C'TCGA_G Cuts at: 1943 2802 1943 Size: 859 6165 XmaI C'CCGG_G Cuts at: 1870 4349 1870 Size: 2479 4545 XmnI GAAnn'nnTTC Cuts at: 2118 5153 2118 Size: 3035 3989 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4832 276 Size: 53 83 186 936 3298 2468 Fragments arranged by size: 3298 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI