(Circular) (Six-base) MAPSORT of: pFN21AA1255.seq Check: 3870 from: 1 to: 8338 pFN21AA1255 8338 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4717 4717 Size: 8338 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5524 6148 278 Size: 53 83 186 936 3988 624 2468 Fragments arranged by size: 3988 2468 936 624 186 83 53 AccI GT'mk_AC Cuts at: 1937 5681 1937 Size: 3744 4594 Acc65I G'GTAC_C Cuts at: 5659 5659 Size: 8338 AclI AA'CG_TT Cuts at: 1563 6465 6838 1563 Size: 4902 373 3063 Fragments arranged by size: 4902 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2360 3859 4289 6401 7785 8053 1632 Size: 728 1499 430 2112 1384 268 1917 Fragments arranged by size: 2112 1917 1499 1384 728 430 268 AfeI AGC'GCT Cuts at: 2228 2749 2228 Size: 521 7817 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8141 AflIII A'CryG_T Cuts at: 1203 3244 4170 7237 8214 1203 Size: 2041 926 3067 977 1327 Fragments arranged by size: 3067 2041 1327 977 926 AgeI A'CCGG_T Cuts at: 7169 7169 Size: 8338 AhdI GACnn_n'nnGTC Cuts at: 3985 7067 3985 Size: 3082 5256 AleI CACnn'nnGTG Cuts at: 1166 2367 1166 Size: 1201 7137 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2385 3216 5285 5288 1184 Size: 1201 831 2069 3 4234 Fragments arranged by size: 4234 2069 1201 831 3 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2513 2545 2513 Size: 32 8306 AlwNI CAG_nnn'CTG Cuts at: 2628 3289 3955 4374 4981 5401 6081 7653 2628 Size: 661 666 419 607 420 680 1572 3313 Fragments arranged by size: 3313 1572 680 666 661 607 420 419 ApaI G_GGCC'C Cuts at: 2086 2086 Size: 8338 ApaLI G'TGCA_C Cuts at: 5113 5467 6395 7551 5113 Size: 354 928 1156 5900 Fragments arranged by size: 5900 1156 928 354 ApoI r'AATT_y Cuts at: 1493 4747 4822 5647 5873 6025 8134 1493 Size: 3254 75 825 226 152 2109 1697 Fragments arranged by size: 3254 2109 1697 825 226 152 75 AseI AT'TA_AT Cuts at: 160 1032 6892 160 Size: 872 5860 1606 Fragments arranged by size: 5860 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8338 AvaI C'yCGr_G Cuts at: 1870 1943 2086 3891 4158 5339 5663 1870 Size: 73 143 1805 267 1181 324 4545 Fragments arranged by size: 4545 1805 1181 324 267 143 73 BamHI G'GATC_C Cuts at: 5668 6035 5668 Size: 367 7971 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2121 3092 3200 3572 3671 Size: 325 217 627 334 971 108 372 99 Cuts at: 3671 4299 4403 4508 5659 7114 618 Size: 628 104 105 1151 1455 1842 Fragments arranged by size: 1842 1455 1151 971 628 627 372 334 325 217 108 105 104 99 BanII G_rGCy'C Cuts at: 1391 2086 3660 3962 4347 5335 5657 1391 Size: 695 1574 302 385 988 322 4072 Fragments arranged by size: 4072 1574 988 695 385 322 302 BbeI G_GCGC'C Cuts at: 3675 4407 3675 Size: 732 7606 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2418 2824 2829 3366 4487 5516 928 Size: 971 519 406 5 537 1121 1029 3750 Fragments arranged by size: 3750 1121 1029 971 537 519 406 5 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2461 2495 6529 6563 1903 Size: 34 50 34 440 34 4034 34 3678 Fragments arranged by size: 4034 3678 440 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5244 6230 7440 5244 Size: 986 1210 6142 Fragments arranged by size: 6142 1210 986 BclI T'GATC_A Cuts at: 1548 2769 4800 1548 Size: 1221 2031 5086 Fragments arranged by size: 5086 2031 1221 BfrBI ATG'CAT Cuts at: 5223 5223 Size: 8338 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2046 2090 2303 Size: 107 122 71 631 605 374 44 213 Cuts at: 2303 6949 136 Size: 4646 1525 Fragments arranged by size: 4646 1525 631 605 374 213 122 107 71 44 BglII A'GATC_T Cuts at: 2899 2911 3031 8333 2899 Size: 12 120 5302 2904 Fragments arranged by size: 5302 2904 120 12 BlpI GC'TnA_GC Cuts at: 4722 5755 4722 Size: 1033 7305 Bme1580I G_kGCm'C Cuts at: 1790 2086 3097 3577 5117 5471 6399 7555 1790 Size: 296 1011 480 1540 354 928 1156 2573 Fragments arranged by size: 2573 1540 1156 1011 928 480 354 296 BmgBI CAC'GTC Cuts at: 1318 2827 4381 1318 Size: 1509 1554 5275 Fragments arranged by size: 5275 1554 1509 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3098 6069 7027 Size: 416 53 83 399 24 1681 2971 958 Cuts at: 7027 442 Size: 1753 Fragments arranged by size: 2971 1753 1681 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8338 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3050 3050 Size: 8338 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2428 2528 2954 3019 3549 3839 3984 4076 Size: 1189 100 426 65 530 290 145 92 Cuts at: 4076 4091 4250 4520 6998 1239 Size: 15 159 270 2478 2579 Fragments arranged by size: 2579 2478 1189 530 426 290 270 159 145 100 92 65 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6463 7328 7626 6463 Size: 865 298 7175 Fragments arranged by size: 7175 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3888 7001 882 Size: 617 2389 3113 2219 Fragments arranged by size: 3113 2389 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4555 6129 493 Size: 711 3351 1574 2702 Fragments arranged by size: 3351 2702 1574 711 BsaBI GATnn'nnATC Cuts at: 2457 8332 2457 Size: 5875 2463 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2130 3672 3764 4404 Size: 53 83 186 936 597 1542 92 640 Cuts at: 4404 5124 5521 6145 6527 7162 275 Size: 720 397 624 382 635 1451 Fragments arranged by size: 1542 1451 936 720 640 635 624 597 397 382 186 92 83 53 BsaWI w'CCGG_w Cuts at: 3796 3868 5322 6771 7169 7443 7590 8001 3796 Size: 72 1454 1449 398 274 147 411 4133 Fragments arranged by size: 4133 1454 1449 411 398 274 147 72 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4908 4938 5423 5453 1178 Size: 30 165 30 3505 30 485 30 4063 Fragments arranged by size: 4063 3505 485 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2567 2669 3042 4176 1186 Size: 1381 102 373 1134 5348 Fragments arranged by size: 5348 1381 1134 373 102 BseYI C'CCAG_C Cuts at: 2859 3907 3948 3955 4278 4971 7541 8037 2859 Size: 1048 41 7 323 693 2570 496 3160 Fragments arranged by size: 3160 2570 1048 693 496 323 41 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2092 2709 3095 3377 3755 3992 4301 Size: 573 356 617 386 282 378 237 309 Cuts at: 4301 4844 5144 1163 Size: 543 300 4357 Fragments arranged by size: 4357 617 573 543 386 378 356 309 300 282 237 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2029 3942 5080 5327 5788 6549 Size: 275 186 28 1913 1138 247 461 761 Cuts at: 6549 7577 1540 Size: 1028 2301 Fragments arranged by size: 2301 1913 1138 1028 761 461 275 247 186 28 BsiHKAI G_wGCw'C Cuts at: 2374 3660 4062 5117 5471 6399 6484 7555 2374 Size: 1286 402 1055 354 928 85 1071 3157 Fragments arranged by size: 3157 1286 1071 1055 928 402 354 85 BsmI GAATG_Cn' Cuts at: 2668 5194 5225 5849 5942 2668 Size: 2526 31 624 93 5064 Fragments arranged by size: 5064 2526 624 93 31 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2086 2374 3097 3577 3660 3962 4062 Size: 399 296 288 723 480 83 302 100 Cuts at: 4062 4347 5117 5335 5471 5657 6399 6484 7555 Size: 285 770 218 136 186 742 85 1071 Cuts at: 7555 1391 Size: 2174 Fragments arranged by size: 2174 1071 770 742 723 480 399 302 296 288 285 218 186 136 100 85 83 BspEI T'CCGG_A Cuts at: 3796 3868 8001 3796 Size: 72 4133 4133 Fragments arranged by size: 4133 4133 72 BspHI T'CATG_A Cuts at: 6227 7957 6227 Size: 1730 6608 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2040 3693 4717 4989 5693 844 Size: 1196 1653 1024 272 704 3489 Fragments arranged by size: 3489 1653 1196 1024 704 272 BsrBI CCG'CTC Cuts at: 1394 1823 6225 1394 Size: 429 4402 3507 Fragments arranged by size: 4402 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6833 7007 65 Size: 1183 5585 174 1396 Fragments arranged by size: 5585 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2028 2060 2115 5079 6982 7169 1700 Size: 110 218 32 55 2964 1903 187 2869 Fragments arranged by size: 2964 2869 1903 218 187 110 55 32 BsrGI T'GTAC_A Cuts at: 96 3126 96 Size: 3030 5308 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8338 BssSI C'ACGA_G Cuts at: 3326 6398 7410 3326 Size: 3072 1012 4254 Fragments arranged by size: 4254 3072 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2170 4529 4981 794 Size: 1376 2359 452 4151 Fragments arranged by size: 4151 2359 1376 452 BstBI TT'CG_AA Cuts at: 3399 7142 3399 Size: 3743 4595 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8338 BstXI CCAn_nnnn'nTGG Cuts at: 2812 2867 2953 4555 2812 Size: 55 86 1602 6595 Fragments arranged by size: 6595 1602 86 55 BstYI r'GATC_y Cuts at: 1972 2490 2899 2911 3031 5668 6035 6420 6437 Size: 518 409 12 120 2637 367 385 17 Cuts at: 6437 7889 7975 7987 8333 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2637 1977 1452 518 409 385 367 346 120 86 17 12 12 Bsu36I CC'TnA_GG Cuts at: 2463 2579 2720 2463 Size: 116 141 8081 Fragments arranged by size: 8081 141 116 BtgI C'CryG_G Cuts at: 513 1065 2015 2077 3411 5031 5438 513 Size: 552 950 62 1334 1620 407 3413 Fragments arranged by size: 3413 1620 1334 950 552 407 62 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4222 4928 487 Size: 683 56 2996 706 3897 Fragments arranged by size: 3897 2996 706 683 56 BtsI GCAGTG_nn' Cuts at: 2404 3107 3299 3360 3767 4375 5156 5469 5512 Size: 703 192 61 407 608 781 313 43 Cuts at: 5512 5854 6648 6668 2404 Size: 342 794 20 4074 Fragments arranged by size: 4074 794 781 703 608 407 342 313 192 61 43 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8338 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8303 DraI TTT'AAA Cuts at: 5642 5642 Size: 8338 DraIII CAC_nnn'GTG Cuts at: 3092 3118 3624 3092 Size: 26 506 7806 Fragments arranged by size: 7806 506 26 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7345 809 Size: 507 6029 1802 Fragments arranged by size: 6029 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2026 2148 2295 Size: 54 1425 50 275 18 196 122 147 Cuts at: 2295 3258 3639 4014 4113 4557 5077 5196 5785 Size: 963 381 375 99 444 520 119 589 Cuts at: 5785 6674 8303 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 963 889 589 520 444 381 375 275 196 147 122 119 99 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2026 5077 5785 1537 Size: 275 214 3051 708 4090 Fragments arranged by size: 4090 3051 708 275 214 EarI CTCTTCn'nnn_ Cuts at: 1353 3843 4052 4419 4473 5061 5216 6268 1353 Size: 2490 209 367 54 588 155 1052 3423 Fragments arranged by size: 3423 2490 1052 588 367 209 155 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2228 3758 6925 7299 7445 387 Size: 1064 138 639 1530 3167 374 146 1280 Fragments arranged by size: 3167 1530 1280 1064 639 374 146 138 EcoICRI GAG'CTC Cuts at: 3658 3658 Size: 8338 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2360 2428 2528 2954 3019 3549 3839 Size: 393 728 68 100 426 65 530 290 Cuts at: 3839 3859 3984 4076 4091 4250 4289 4520 6401 Size: 20 125 92 15 159 39 231 1881 Cuts at: 6401 6998 7785 8053 1239 Size: 597 787 268 1524 Fragments arranged by size: 1881 1524 787 728 597 530 426 393 290 268 231 159 125 100 92 68 65 39 20 15 EcoNI CCTnn'n_nnAGG Cuts at: 2723 3191 2723 Size: 468 7870 EcoO109I rG'GnC_Cy Cuts at: 2052 2082 2083 3066 3184 4289 4978 2052 Size: 30 1 983 118 1105 689 5412 Fragments arranged by size: 5412 1105 983 689 118 30 1 EcoRI G'AATT_C Cuts at: 4822 5647 6025 4822 Size: 825 378 7135 Fragments arranged by size: 7135 825 378 EcoRV GAT'ATC Cuts at: 4612 4612 Size: 8338 FspI TGC'GCA Cuts at: 2247 6844 2247 Size: 4597 3741 HaeII r_GCGC'y Cuts at: 1678 2230 2751 3675 4407 7485 1678 Size: 552 521 924 732 3078 2531 Fragments arranged by size: 3078 2531 924 732 552 521 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3143 3175 3710 3742 3791 Size: 32 135 32 1571 32 535 32 49 Cuts at: 3791 3823 3957 3971 3989 4003 4226 4258 5352 Size: 32 134 14 18 14 223 32 1094 Cuts at: 5352 5384 5408 5440 6979 7011 7053 7085 1373 Size: 32 24 32 1539 32 42 32 2626 Fragments arranged by size: 2626 1571 1539 1094 535 223 135 134 49 42 32 32 32 32 32 32 32 32 32 32 24 18 14 14 HincII GTy'rAC Cuts at: 669 1657 1938 3076 3583 4851 5365 5682 5928 Size: 988 281 1138 507 1268 514 317 246 Cuts at: 5928 669 Size: 3079 Fragments arranged by size: 3079 1268 1138 988 514 507 317 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8338 HpaI GTT'AAC Cuts at: 5928 5928 Size: 8338 KasI G'GCGC_C Cuts at: 3671 4403 3671 Size: 732 7606 KpnI G_GTAC'C Cuts at: 5663 5663 Size: 8338 MfeI C'AATT_G Cuts at: 5937 5937 Size: 8338 MluI A'CGCG_T Cuts at: 8214 8214 Size: 8338 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2136 2421 3969 5431 7452 7636 1305 Size: 831 285 1548 1462 2021 184 2007 Fragments arranged by size: 2021 2007 1548 1462 831 285 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2150 2297 3641 4016 4115 Size: 54 1425 343 318 147 1344 375 99 Cuts at: 4115 4559 10 Size: 444 3789 Fragments arranged by size: 3789 1425 1344 444 375 343 318 147 99 54 MslI CAynn'nnrTG Cuts at: 518 1166 2367 2675 2810 3798 4175 4263 4553 Size: 648 1201 308 135 988 377 88 290 Cuts at: 4553 4620 4815 6296 6655 6814 518 Size: 67 195 1481 359 159 2042 Fragments arranged by size: 2042 1481 1201 988 648 377 359 308 290 195 159 135 88 67 MspA1I CmG'CkG Cuts at: 1606 2017 2079 2406 3286 3520 3527 3952 4024 Size: 411 62 327 880 234 7 425 72 Cuts at: 4024 4111 4204 4591 5195 5754 6431 7579 7824 Size: 87 93 387 604 559 677 1148 245 Cuts at: 7824 1606 Size: 2120 Fragments arranged by size: 2120 1148 880 677 604 559 425 411 387 327 245 234 93 87 72 62 7 NaeI GCC'GGC Cuts at: 2030 2117 5081 2030 Size: 87 2964 5287 Fragments arranged by size: 5287 2964 87 NarI GG'CG_CC Cuts at: 3672 4404 3672 Size: 732 7606 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7786 NdeI CA'TA_TG Cuts at: 387 4151 387 Size: 3764 4574 NgoMIV G'CCGG_C Cuts at: 2028 2115 5079 2028 Size: 87 2964 5287 Fragments arranged by size: 5287 2964 87 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8338 NotI GC'GGCC_GC Cuts at: 5785 5785 Size: 8338 NsiI A_TGCA'T Cuts at: 5225 5225 Size: 8338 NspI r_CATG'y Cuts at: 1132 1229 3217 3248 3934 4174 4278 5227 5696 Size: 97 1988 31 686 240 104 949 469 Cuts at: 5696 7241 1132 Size: 1545 2229 Fragments arranged by size: 2229 1988 1545 949 686 469 240 104 97 31 PasI CC'CwG_GG Cuts at: 1348 2867 1348 Size: 1519 6819 PciI A'CATG_T Cuts at: 3244 4170 7237 3244 Size: 926 3067 4345 Fragments arranged by size: 4345 3067 926 PflMI CCAn_nnn'nTGG Cuts at: 1108 3955 4278 1108 Size: 2847 323 5168 Fragments arranged by size: 5168 2847 323 PfoI T'CCnGG_A Cuts at: 3816 4285 4833 3816 Size: 469 548 7321 Fragments arranged by size: 7321 548 469 PmeI GTTT'AAAC Cuts at: 5642 5642 Size: 8338 PmlI CAC'GTG Cuts at: 4555 4555 Size: 8338 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2495 2527 6407 6439 7920 7952 2495 Size: 32 3880 32 1481 32 2881 Fragments arranged by size: 3880 2881 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2052 4289 4978 2052 Size: 2237 689 5412 Fragments arranged by size: 5412 2237 689 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8338 PsiI TTA'TAA Cuts at: 5564 5908 5564 Size: 344 7994 PspOMI G'GGCC_C Cuts at: 2082 2082 Size: 8338 PstI C_TGCA'G Cuts at: 830 2202 3195 3294 3666 3690 3762 5690 830 Size: 1372 993 99 372 24 72 1928 3478 Fragments arranged by size: 3478 1928 1372 993 372 99 72 24 PvuI CG_AT'CG Cuts at: 2001 3942 2001 Size: 1941 6397 PvuII CAG'CTG Cuts at: 2406 3286 3527 3952 4111 4591 2406 Size: 880 241 425 159 480 6153 Fragments arranged by size: 6153 880 480 425 241 159 SacI G_AGCT'C Cuts at: 3660 3660 Size: 8338 SacII CC_GC'GG Cuts at: 2018 2080 2018 Size: 62 8276 SalI G'TCGA_C Cuts at: 1936 5680 1936 Size: 3744 4594 SapI GCTCTTCn'nnn_ Cuts at: 3843 5061 3843 Size: 1218 7120 SbfI CC_TGCA'GG Cuts at: 5690 5690 Size: 8338 ScaI AGT'ACT Cuts at: 6586 8298 6586 Size: 1712 6626 SexAI A'CCwGG_T Cuts at: 4291 5379 4291 Size: 1088 7250 SfcI C'TryA_G Cuts at: 826 1045 2198 2570 3191 3290 3515 3662 3686 Size: 219 1153 372 621 99 225 147 24 Cuts at: 3686 3758 5686 6821 7502 7693 826 Size: 72 1928 1135 681 191 1471 Fragments arranged by size: 1928 1471 1153 1135 681 621 372 225 219 191 147 99 72 24 SfoI GGC'GCC Cuts at: 3673 4405 3673 Size: 732 7606 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8338 SgrAI Cr'CCGG_yG Cuts at: 1700 2060 1700 Size: 360 7978 SmaI CCC'GGG Cuts at: 1872 5665 1872 Size: 3793 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 6442 7343 7605 820 Size: 197 926 4499 901 262 1553 Fragments arranged by size: 4499 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6129 493 Size: 5636 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8338 SphI G_CATG'C Cuts at: 1132 3934 5227 5696 1132 Size: 2802 1293 469 3774 Fragments arranged by size: 3774 2802 1293 469 SspI AAT'ATT Cuts at: 5 52 6262 5 Size: 47 6210 2081 Fragments arranged by size: 6210 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8338 StyI C'CwwG_G Cuts at: 513 1065 5427 5547 5777 513 Size: 552 4362 120 230 3074 Fragments arranged by size: 4362 3074 552 230 120 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3080 4287 5615 6365 6382 6535 6720 Size: 1255 1500 1207 1328 750 17 153 185 Cuts at: 6720 7165 325 Size: 445 1498 Fragments arranged by size: 1500 1498 1328 1255 1207 750 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2328 3126 4486 Size: 274 80 33 51 1443 351 798 1360 Cuts at: 4486 5614 6584 8296 96 Size: 1128 970 1712 138 Fragments arranged by size: 1712 1443 1360 1128 970 798 351 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2130 2162 4908 4940 5423 5455 8298 8330 2130 Size: 32 2746 32 483 32 2843 32 2138 Fragments arranged by size: 2843 2746 2138 483 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2828 3749 1316 Size: 1512 921 5905 Fragments arranged by size: 5905 1512 921 XbaI T'CTAG_A Cuts at: 5674 5674 Size: 8338 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2437 2437 Size: 8338 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8338 XmaI C'CCGG_G Cuts at: 1870 5663 1870 Size: 3793 4545 XmnI GAAnn'nnTTC Cuts at: 3395 6467 3395 Size: 3072 5266 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5522 6146 276 Size: 53 83 186 936 3988 624 2468 Fragments arranged by size: 3988 2468 936 624 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbvCI Bpu10I BsiWI BsmBI BstZ17I FalI FseI FspAI NruI PacI PspXI PsrI RsrII SanDI SfiI SrfI SwaI